A 28 days growth and development experiment and a 90 days intoxication experiment with —hydrochloro lysine at various dosage levels on young and adult rats were reported.The results were as follows. There were definitely an accelerating effect on growth and development in young rats when adding 0.5% L-lysine to a cereal diet, but no obviously similar effect in adult rats; moreover, a massive dose of L—lysine could induce hepatic and renal damage. The hepatic lesions were focal necrosis and increase of fat—storing cells with collagen fiber formation. In the kidney there were cloudy swelling, microvilli losing, intracellular edema, mitochondrial swelling, endopl asmic reticulum dilatation and residual body formation in the proximal convoluted tubule epithelium.

Objective To investigate the neuroprotective effect of soluble epoxide hydrolase (sEH) inhibitor 12-(3-adamantan-l-yl-ureido) dodecanoic acid (AUDA) on focal cerebral ischemia/reperfusion in rats and its mechanisms.Methods Sixty male Sprague-Dawley rats were randomly divided into sham operation and saline control groups,as well as low-dose (0.157 ml/kg),medium-dose (0.235 ml/kg) and high-dose (0.314 ml/kg) AUDA groups (n =12 in each group).Four rats in each group were selected for infarct volume,cell apoptosis and p-Akt immunohistochemistry detection.A model of middle cerebral artery ischemia/ reperfusion was induced by the suture method.The corresponding dose AUDA or equal volume of saline was injected intraperitoneally before reperfusion in each AUDA group and the saline control group.Neurological deficit scores were performed at 24 h of reperfusion.2,3,5 triphenyltetrazolium chloride (TTC) staining was used to detect infarct volume.TdT-mediated dUTP nick end labeling (TUNEL) was used to detect apoptotic cells of brain tissue in the periinfarction area.Immunohistochemical method was used to detect p-Akt expression of brain tissue in the peri-infarction area.Results TTC staining showed no infarction was observed in the sham operation group.The infarction volumes in the saline control group as well as the low-dose,medlum-dose and high-dose AUDA groups were 254.146 ± 25.481,212.679 ± 7.514,150.188 ± 33.997,and 99.563 ± 3.415 mm3,respectively.There were significant differences (F =39.637,P =0.000).The each dose AUDA group was significant less than the control group (all P=0.000).The medium-dose AUDA group was significantly less than the low-dose AUDA group (P=0.002),and the high-dose AUDA group was also significantly less than the low-dose AUDA group (P =0.000) and medium-dose AUDA group (P =0.006).TUNEL staining showed that a small number of apoptotic cells (6.400 ± 1.477/high-power field) were observed in the sham operation group.The numbers of apoptotic cells in the saline control group as well as in the low-dose,medium-dose and high-dose AUDA groups were 57.550 ± 13.067,47.030 ± 8.423,34.530 ± 4.393 and 26.400 ± 2.683/high power field,respectively.Each dose AUDA group was significantly less than the saline control group (all P ＜0.01).The medium-dose and high-dose AUDA groups were significantly less than the low-dose AUDA group (P ＜ 0.01),and the high-dose AUDA group was also significantly less than the medium-dose AUDA group (P ＜0.01).Immunohistochemistry showed that only a few p-Akt-positive cells (3.325 ± 1.438/high power field) were observed in the sham operation group.The numbers of p-Akt-positive cells in the saline control group as well as the low-dose,medium-dose and high-dose AUDA groups were 9.450 ±2.531,16.400 ± 3.865,22.875 ± 7.974,and 29.300 ± 3.203/high-power field,respectively.Each dose AUDA group was significantly more than the saline control group (all P ＜0.01).The medium-dose and high-dose AUDA groups were significantly more than the low-dose AUDA group (all P ＜0.01).The high-dose AUDA group was also significantly more than the medium-dose AUDA group (P ＜ 0.01).Conclusions The inhibition of sEH may decrease neuronal apoptosis and reduce infarct volume in the peri-infarction area by upregulating the PI3K/Akt pathway.It has a neuroprotective effect for focal cerebral ischemia/reperfusion in rats.

Fiber bronchoscopy has been used in pediatric for decades,and has became the important diagnostic tools in pediatric respiratory diseases.with the improvement of fiber bronchoscope equipment and the advancement of operation technology,fiber bronchoscope has been expanded unceasingly in pediatric, especially in the critically ill children.As a common critical disease in pediatric,severe pneumonia is urgent, serious and high rate of mortality.As the effect of conventional antibiotic treatment is not ideal in current, while fiber bronchoscopy with the dual function of examination and treatment makes it use more and more widely in children with severe pneumonia.Fiber bronchoscopy can improve the effect of treatment in children with severe pneumonia,short the course of the disease,improve the prognosis of children,reduce the rate of mortality.

The incidence of local recurrence after wide local excision and radiation of soft tissue sarcoma (STS) ranges from 5％ to 20％.The optimal management of locally recurrent STS must be individualized.Approaches for retreatment include wide local re-excision followed by a variety of radiation which include external-beam radiation,brachytherapy and intraoperative electron radiotherapy.Following retreatment,the likelihood of ultimate local control ranges from 37％ to 100％.However,each radiation technique could produce severe side effects,and so for selected patients,repeat irradiation may be unnecessary.

Objective To study on the correlation of hepatic fibrosis with angiotensin Ⅱ (Ang Ⅱ ) in the hepatitis B patients . Methods The electrochemical luminescence and the rate method were adopted to detect hyaluronidase (HA) and Ang Ⅱ levels in 92 cases of acute hepatitis B (acute group) ,108 cases of chronic hepatitis B(chronic group) and 50 healthy people(control group) , and the detected results were performed the correlation analysis .Results Compared with the acute group and the control group ,the HA and Ang Ⅱ levels in the chronic group were increased significantly (P<0 .05) .The HA and Ang Ⅱ levels had statistical differ-ence between the acute group and the control group (P<0 .05) .Further analysis showed that there was a positive correlation be-tween HA and Ang Ⅱ levels(r=0 .629 ,P<0 .05) .Conclusion There the HA level is closely related with the Ang Ⅱ level ,and both of them participate in the process of liver fibrosis ,their levels have the guiding significance in evaluation of severity of liver fi-brosis in the patients with hepatitis B .

IL-18 participates in the morbility and exacerbation of acute pancreatitis,and induces the organ-injury of extra-pancreas.The aim of this review is to explore the source of and the regulation of activation and secretion of IL-18,as well as the pathogenesis of acute pancreatitis-associated liver injury induced by IL-18.

Breast tumor is the most common malignant tumor in female,its incidence is increasing in recent years.With magnetic resonance equipment,image acquisition and processing technology is improved,perfusion imaging studies gradually become the focus of study.The author reviewed MRI perfusion imaging and its application in breast tumors.

Objective To explore the treatment of kala-azar with antimonial resistance in children. Method The clinical data of antimony resistant Kala Azar in a child was analyzed retrospectively, and the related literature were reviewed. Results A 2-year- and 5-month-old boy, suffered from fever, pancytopenia and hepatosplenomegaly. He was diagnosed with kala-azar by bone marrow examination, and improved after the treatment of adequate antimonial. Later, he relapsed twice and the treatment of adequate was effective.. This was the third relapse, and was considered as antimonial resistance. Liposomal amphotericin B (1 mg/kg on day 1, 2 mg/kg on day 2, 3mg/kg on day 3~7 and day 10, with accumulated dose of 21 mg/kg) was used and effective. The child improved and no relapse in one-year follow-up. Conclusion Liposomal amphotericin B can be used in the treatment of kala-azar with antimonial resistance in children.

Diagnosis, Differential ; Humans ; Lung Diseases, Interstitial ; diagnosis ; pathology ; Pathology, Clinical

[Objective] The better optimization of traditional Chinese medicine for the treatment of respiratory syncytial virus, in the past five years is re-viewed in this paper, also the related domestic experimental research results, and according to the pathogenesis of RSV, it further summarizes the current domestic research for the main direction of the disease. [Methods] The article from the two aspects of compound Chinese medicine and traditional Chinese medicine monomer, according to the pathogenesis of respiratory syncytial virus section, discusses the experiment in vivo and in vitro related rich research results. [Results] It is found by inducing and summarizing, whether individual or compound traditional Chinese medicine, for respiratory syncytial virus re-search has made great achievement, but with experimental study limited to suppress the virus more value-added; replication and inhibiting inflammatory infiltrates the two big aspects, lacking of deeper side of molecular biology research, making the traditional Chinese medicine in the treatment of respiratory syncytial virus research appear too single, not a broader recognition by the industry. [Conclusion] We need to be sure the safety of traditional Chinese medicine for the treatment of respiratory syncytial virus and curative effect, but at the same time must recognize that because of the complexity of its com-position and design of experiment of traditional Chinese medicine, the irrationality of the existence of many factors, it makes progress with many problems, so we must be aware of their own insufficiency, more in-depth explore its mechanism of action, with al kinds of research direction in the future for us.