Object To determine the change of benzoic acid contents in decoctions prepared from various combinations of the recipe named SIWUTANG by RP HPLC to provide a reference for the control of the acid level Methods 8 decoctions of P lactiflora either alone or in combination with one or more of the other 3 components, according to permutation and combination, were prepared and their benzoic acid contents determined by RP HPLC Results The content of benzoic acid in the decoction of Radix Paeonie Alba alone was found to be 2 74 mg/g, while that of the other 7 combinations varied from 0 41～1 11 mg/g Conclusion The content of benzoic acid in SIWUTANG could be lowered significantly by the presence of Radix Angelica Sinensis, Radix Rehmanniae, and Rhizoma Chuanxiong

Abstract: Objective　To analyze the vaccination status of SARS-CoV-2 in children, and explore the relationship between SARS-CoV-2 vaccination and COVID-19 in children. Methods　A retrospective study was conducted to analyze the clinical data of 335 cases of SARS-CoV-2 Omicron variant infection from February 15, 2022 to March 18, 2022 in Shenzhen Third People's Hospital. Results　Among 335 children with SARS-CoV-2 infection, 174(51.9%) cases were vaccinated with the SARS-CoV-2 vaccine; 33(31.4%) cases were vaccinated in the 3-<6 years old group; 141(61.3%) cases were vaccinated in the 6-<14 years old group. There was a statistically significant difference in the proportion of SARS-CoV-2 vaccination between the 6-<14 years old group and the 3-<6 years old group (χ2=26.1, P<0.05). In the study cohort, 3-<6 years old group and 6-<14 years old group, there was no significant difference in the incidence of COVID-19 in the vaccinated group compared with the unvaccinated group (P>0.05). In the study cohort, the proportion of confirmed cases of 1 dose of SARS-CoV-2 vaccine and 2 doses or more of SARS-CoV-2 vaccine was 89.5% (68 cases) and 77.6% (76 cases), respectively; in the 6~<14 years old group, the proportion of confirmed cases of 1 dose of SARS-CoV-2 vaccine and 2 doses or more of SARS-CoV-2 vaccine was 90.0% (54 cases) and 76.5% (62 cases), respectively; the differences were statistically significant (χ2=4.264, P<0.05; χ2=4.279，P<0.05). The IgG levels of 18.28 (6.61, 55.2) AU/mL and 58.3 (25.85, 131.41) AU/mL in the study cohort who were vaccinated for 1 dose, 2 doses and more, respectively; the IgG levels of 20.13 (8.33, 44.33) AU/mL and 56.57 (25.85, 150.07) AU/mL in the 6~<14 years old group who were vaccinated for 1 dose, 2 doses and more, respectively; and the differences were statistically significant (Z=-4.37, P<0.05; Z=-3.96, P<0.05). Conclusions　Children who received 2 doses of SARS-CoV-2 vaccine have a lower incidence of COVID-19 and higher levels of SARS-CoV-2 antibodies compared with who received 1 dose . It is recommended that children are advised to be vaccinated against the COVID-19.

An adjustable pillow with inflatable cushion and soft polyurethane sponge is introduced.It is not easily deformed and the height is adjustable.In addition,it is easy to carry and can be cleaned and disinfected thoroughly.Though the design is quite simple,this kind of pillow can improve the patients' sleep quality and prevent pollution of pillow as a result of iatrogenic infection.

Objective To synthesize of water-solubility derivant of 6,7,4′- trihydroxyisoflavone(T2 ),to characterize its structure and to evaluate its anti-tumor activity.Methods The sulfonic group(-SO3 H)was grafted to 3'position in loop B of T2 through sulfonation reaction,then strong aqua was added to above production,and obtained a water-solubility elevated compound named T2-SO3 (NH4 )2 through ammoniation.The construction of T2-SO3 (NH4 )2 was characterized by 1 H-NMR,13 C NMR,MS and element analysis.Its activity for killing human breast cancer cells (Hela)was analyzed by CCK-8 assay and flow cytometry.Re-sults The two kinds of water-solubility derivant of T2-SO3 H·2H2 O and T2-SO3 (NH4 )2 were obtained through above methods, and their yield rates were 96% and 75% respectively.The biological experiments showed that the anti-tumor activity of T2-SO3 (NH4 )2 was significantly enhanced.Conclusion Compared to T2,T2-SO3 (NH4 )2 exhibit higher biocompatibility and anti-tumor activity with vast biological application prospect.

Based on collecting data at home and abroad, we combined clinical practice of scientific researches. We also summarized key points for design and evaluation of clinical studies in treating children's attention deficit hyperactivity disorder by Chinese medical new drugs from objective and design, selection of diagnostic criteria, recruitment and dropping-out of subjects, effectiveness evaluation, safety evaluation, drug combination, and quality control, and so on. We hope to provide reference for design and evaluation of clinical studies by Chinese medical new drugs.
Attention Deficit Disorder with Hyperactivity ; drug therapy ; Biomedical Research ; Child ; Clinical Studies as Topic ; Data Collection ; Drugs, Chinese Herbal ; therapeutic use ; Humans

To discuss the etiology, pathogenesis, therapies and prescriptions of psycho-cardiological abnormality of patients with coronary heart disease. According to the advance in modern diagnosis and treatment, the authors believed that psycho-cardiological abnormality of patients with coronary heart disease is closely related with mental stresses, like anxiety, depression and insomnia. It is mostly caused by emotional injury and expressed in heart, liver, spleen and kidney. The pathogenesis is heart-liver hyperactivity, yin deficiency in heart and kidney, and insufficiency in heart and spleen. The full recognition of etiology and pathogenesis of psycho-cardiological abnormality of patients with coronary heart disease and the combined treatment of disease and syndromes are of great significance to reduce mental stress and other risk factors, prevent and treat coronary heart disease and improve prognosis.
Coronary Disease ; drug therapy ; psychology ; Humans ; Medicine, Chinese Traditional ; Stress, Psychological ; drug therapy ; etiology

Objective To compare the efficacy of different antibiotics strategy,introperitoneal (IP) cefazolin plus third-generation cephalosporin versus IP Vancomycin plus thirdgeneration cephalosporin on peritoneal dialysis (PD)-related peritonitis.Methods All episodes of PD -associated peritonitis happened in prevalent PD patients between January 2008 and December 2012 were recruited from the PD Center of Peking University First Hospital.According to their empiric antibiotics scheme,episodes were divided into group A (where IP cefazolin plus third-generation cephalosporins were administrated) and group B (where IP Vancomycin plus third-generation cephalosporins were administrated).Multivariable logistic regression model was used to explore the influence of different emnpiric antibiotics scheme on peritonitis outcome.Results Patients in Group B had significantly lower level of serum albumin (33.5±6.0 vs 35.3±5.2 g/L) and cholesterol (4.6±1.3 vs 4.9± 1.1 mmol/L) than those in group A.In group A,the percentage of gram-positive bacteria was similar to group B (43.2％ vs 43.3％,P =0.96),but gram-negative bacteria was numerically lower (16.9％ vs 24.7％,P =0.08).Different empiric antibiotics strategy was not independent predictor of peritonitis outcome [OR =1.07,95％ CI(0.45,2.56),P=0.87].Conclusion Both cefazolin and vancomycin can be selected as first-line empiric antibiotic covering gram-positive organisms in the treatment of PD related peritonitis.

To construct recombinant reporter plasmids containing different Alpha gene segments and Alpha1-TFEB fusion gene and to evaluate the promoter activity of the Alpha gene. Methods:Promoter regions of the Alpha gene were predicted using a software Primer 0.5. Five Alpha gene segments with different lengths and a normal TFEB gene promoter (pTFEB) were amplified via polymerase chain reaction, and recombinant reporter plasmids containing different Alpha gene segments and a normal TFEB gene pro-moter were constructed. Liposome transfection was used to transfect these vectors into the human embryo kidney 293T cells. The pro-moter activity of the Alpha gene was evaluated via luciferase assay. Meanwhile, the recombinant Alpha1-TFEB plasmid was construct-ed and transfected into the 293T cells. The TFEB expression of the recombinant Alpha1-TFEB plasmid was then detected via Western blot. Results: Recombinant reporter plasmids containing different Alpha gene segments and pTFEB were constructed successfully. Compared with the luciferase activity of pGL3-Basic, that of the groups with Alpha1, Alpha2, Alpha3, Alpha4 and Alpha5 significantly increased (P<0.01). The luciferase activity also increased significantly in the groups with Alpha1, Alpha2 and Alpha5 compared with that of the pTFEB group (P<0.01). The TFEB expression of the pGL3-Enhancer-Alpha1-TFEB was significantly higher compared with that of the pGL3-Enhancer group. Conclusion:In t(6;11) translocation RCC, the Alpha gene has a strong promoter activity and it en-hances TFEB expression. The strongest promoter activity region is in Alpha5 with a sequence from 643 bp to 693 bp.

Object To study IR absorption spectra of the extracts from chicken bile possessed excellent anti inflammatory and anticancer activity so as to develop this valuable resource.Methods Bile samples from chickens and various animals were dried in the dark and subjected to IR analysis respectively. Results By comparison, significant difference was found between IR spectra of chicken bile and other animals' bile, but the IR spectra of various chicken bile showed very good consistency. Conclusion The IR spectra of chicken bile is independent of raising places, species, feeding fashion, sampling season and age of sampled chicken. It was suggested that the bile of various chicken contained same chemical composition and this result was confirmed by UV and TLC analysis.

Asaprimarilyused plasticizerin polyvinyl chloride,d(i2-ethylhexy)lphthalate(DEHP)is extensively used in consumer products,flooring and wall coverings,food contact applications,and medical devices.Because DEHP has been detected in waters,soils and air,and its metabolites have also been detected in human urinary,sufficient evidences show that DEHP is ubiquitous in the environment worldwide.DEHP is already in the blacklist of priority pollutants in water in China.Many researches indicate that DEHP is one kind of endocrine disrupting chemicals in animals,with carcinogenicity,teratogenicity and mutagenicity.Based on the researches and review on DEHP,this paper principally discussed the environmental level,metabolism and biological markers of DEHP,as well as the research progress on human health effects.