Recent studies have revealed that obease increases the risk of differentated thyroid carcino-ma (DTC);iodine increases the risk of papillary thyroid carcinoma wheras decreases the risk of follicular thy-roid carcinoma;vitamin D decreases the risk of DTC,but there are not enough evidences to prove that vitamin A,C and E can decrease the risk of DTC;alcohol may decrease the risk of DTC,but the mechanism is still un-known;xenobiotics increases the risk of DTC.It is essential for DTC prevention to clarify the protection and risk factors.

Objective To investigate the effects of the different concentration of mineral trioxide aggregate (MTA) on the proliferation and differentiation of dental pulp stem cells (DPSCs) from the young permanent teeth.Methods DPSCs were isolated from the young permanent teeth and cultured by tissue explant method.The expression of STRO-1 was detected by using immunofluorescence technology.DPSCs were cultured with different concentrations of MTA (0.02,0.20,2.00,20.00 g/L).Cell proliferation was detected by MTT array.Cells were cultured in the appropriate concentration of MTA for 4 weeks,and then stained by Alizarin red to detect their mineralized nodule formation capacity.The cells were cultured with the appropriate concentration of MTA and collected after 12,24,36,48 h.The mRNA expression of ALP,BSP,OC and DSP after the treatment of MTA were detected by quantitative PCR.Results DPSCs were positive for STRO-1.The capacity of 0.20 g/L MTA promoting the proliferation of DPSCs was stronger than other concentrations.After 4 weeks,the mineralized nodules of DPSCs were observed after alizarin red staining.The PCR showed that with increasing induction time,the expression levels of DSP and OC were up-regulated.But that of ALP and BSP was increased first and then decreased.Conclusions In this study,MTA can promote the proliferation of DPSCs at 0.02,0.20,2.00 g/L concentration.It can induce odontoblast differentiation effectively by 0.20 g/L MTA.

Objective: In order to implement effectively on-scene medical response to a nuclear or radiological emergency, and also keep track of relevant on-scene audio and video information and monitoring data, we have designed and developed a monitoring platform for medical response to nuclear or radiological emergency. Methods:Based on relevant national standards for emergency response and the features of nuclear or radiological emergency, exploiting modern information technology and monitoring equipments, we formulate the design idea, characteristics, functions and framework of the platform. This platform takes treatment centers for nuclear or radiological injuries, designated medical institutions and medical response teams as main users. Results:We have developed the monitoring platform for medical response to nuclear or radiological emergency. Conclusion:Through the application of this platform, we hope to enhance the preparedness and on-scene response capability for nuclear or radiological emergency, and also provide information support for disposal of nuclear or radiological emergency in the future.

A total of 12 775 SSRs were identified from Scutellaria baicalensis Georgi genomic database, accounting for 2.56% of the total genomic sequences. The result showed that S. baicalensis SSRs were based on 68.32% dinucleotide and 18.63% trinucleotide repeats; CT/GA and TTC/GAA were predominant in the dinucleotide motifs and the trinucleotide motifs respectively. Nine primers were selected to produce highly reproducible SSR bands and were used in studying the genetic diversity of S. baicalensis, 50 individuals from ten populations. 68 SSR polymorphic loci were detected, these loci were polymorphic and displayed 4 to 12 alleles per locus with a mean number of 7; the effect number of alleles was 3. Expected heterozygosities were 0.6 and were far more greater than the average in dicotyledonous plants. PIC (polymorphism information content) was 0.72, Shannon's information index was 1.32, these all proved that S. baicalensis had a high genetic diversity in general. Genetic differentiation among population Gst was 0.131, genetic variation among population accounted for 13.1% and genetic variation within population accounted for 86.9%. The cluster analysis showed that 10 populations S. Baicalensis were classified into 2 groups, but it was not associated with geographical distribution.

OBJECTIVE:To establish a method for the contents determination of 4 amino acids in Pyrrosia lingua. METHODS:The samples were analyzed by RP-HPLC. It was performed on a column of Welchrom C18 with the mobile phase A of 0.1 mol/L So-dium acetate buffer(pH6.5)- acetonitrile(93∶7,V/V)and mobile phase B of acetonitrile-water(4∶1,V/V)(gradient elution)at the flow rate of 1.0 ml/min,detection wavelength was 254 nm,column temperature was 30 ℃ and the sample size was 5 μl. RE-SULTS:The linear range was 4.92-49.2 μg/ml for glutamate(r=0.999 1),3.168-31.68 μg/ml for glycine(r=0.999 9),2.6-26 μg/ml for alanine(r=0.999 4)and 2.88-28.8 μg/ml for leucine(r=0.999 9);RSDs of precision,stability and reproducibility tests were no more than 2.2%;average recoveries were 98.4%(RSD=2.2%,n=9),99.6%(RSD=2.5%,n=9),98.3%(RSD=2.2%,n=9)and 100.1%(RSD=2.3%,n=9),respectively. CONCLUSIONS:The method is simple,accurate and reproducible,and can be used for the contents determination of 4 amino acids in P. lingua.

Choroidal metastasis is one of the most common malignant tumors inside the eyes. It causes pain, hypopsia and some other related symptoms. It reduces the quality of the patients' life. It's significant for the patients to be detected and treated early, therefore they will have better vision and longer life. The treatments of choroidal metastasis are developing quickly. Both the vitreous cavity injection of targeted drug and gene therapy are hot topics of research. This paper summarizes the etiology, development, diagnosis and treatment of choroidal metastasis nowadays.

Objective To compare the results between double-threaded compression screws and cannulated lag screws for Garden type Ⅰ/Ⅱ femoral neck fractures.Methods Data of 56 patients with Garden type Ⅰ/Ⅱ femoral neck fractures who were treated with internal fixation from July 2012 to March 2016 were retrospectively analyzed.According to different fixation,the patients were divided into two groups:double-threaded screws group (group A) including 27 cases (7 males and 20 females,with an average age of 55.5±10.0 years,14 cases Garden type Ⅰ and 13 cases Garden type Ⅱ,the proportion of osteoporosis 10/27;And lag screws group (group B) including 29 cases (8 males and 21 females,with an average age of 57.2±9.5 years,11 cases Garden type Ⅰ and 18 cases Garden type Ⅱ,the proportion of osteoporosis 15/29.The 2 groups were compared with respect to bony fusion time,nonunion,avascular necrosis of femoral head,shortening of femoral neck and Harris Hip Score.Results The average follow-up time was 20.3±8.7 m in Group A and 24.5±7.9 m in Group B.The average bony fusion time was 5.3±0.6 m in Group A and 4.1±0.8 m in Group B,and there was significant difference between the groups (t=5.758,P=0.00).1 avascular necrosis of femoral head was found in both groups.In group A,there were 3 cases of bone nonunion (11.1％,3/27).There were one bone nonunion (3.4％,1/29) case in group B,with no statistically significant difference between groups.Shortening of femoral neck occurred in both groups,the average horizontal shortening was 5.8±2.9 mm and vertical shortening 7.0±3.4 mm in Group A,which were lower than group B (8.2±3.1 mm,9.4±3.6 mm),and the differences showed statistically significant (t=-2.94,P=0.005;t=-2.48,P=0.016).In group A,the average Harris Hip score was 88.2±8.3,while that in group B was 89.9±7.2.There was no statistically significant difference between two groups.Conclusion It seems to us that in the treatment of Garden type Ⅰ/Ⅱ femoral neck fractures,doublethreaded compression screws fixation could reduce femoral neck shortening,but there is no difference on functional outcome compared to cannulated lag screws fixation.On the contrary,double-threaded compression screws fixation is not conducive to fracture healing due to lack of sliding compression.

Objective To evaluate the clinical significance of 18F-FDG PET/CT in localization and etiologic diagnosis of suspected pacemaker-related infection(SPRI).Methods Fifteen SPRI patients (10males,5 females,age range:45-86 years) were included in this retrospectively study.18F-FDG uptake in device-related region and abnormal 18F-FDG uptake out of the device region were visually interpreted.Final diagnosis was decided according to modified Duke's criteria and imaging interpretation.Diagnostic efficiency of 18 F-FDG PET/CT on SPRI was evaluated.Results Of 7 patients with suspected pocket infection,5 patients had hot spot of 18F-FDG in the pocket site,either in superficial skin and/or deep soft tissue.Of 9 patients with suspected infective endocarditis,a diffuse FDG uptake was demonstrated in 3 patients in the area of left atrium,right atrium,and right ventricle;another 3 patients had abnormal FDG uptake in other sites rather than in the implantation regions,and the rest 3 showed negative PET/CT results.In patients with SPRI,18F-FDG PET/CT diagnosed 7 cases of pocket infection,8 cases of infective endocarditis,14 cases of pacemaker-related infection.Conclusions 18F-FDG PET/CT imaging is useful in localization and etiologic diagnosis in SPRI patients.It might guide the clinical SPRI management.

[Objective]To investigate the effect of transforming growth factor ?1(TGF-?1) genes transfection on the proliferation and differentiation of chondrocyte by adenovirus vector,and to observe the quality in repair cartilage defect through constructing tissue-engineered cartilage.[Method]Using replicat ion defective adenovirus Adeno-X~(TM) as a carrier,Articular chondrocytes were transferred by high titers level recombinant adenovirus taking TGF-?1 genes.Ultromicrostructure,prolife ration anddifferentiation were observed by light and electron microscope,flow cytometry and so on.Induce expression of exogenous genes coding protein and cartilage cell phenotype were detected by Immunocytochemistry and Northern blot.Gross observation,histology and effect classification were used to evaluate results in repair cartilage defect by combi nation of infected chondrocytes and bone matrix gelatin(BMG).[Result]Immunocytochemistric staining showedthe expression of exogenous gene coding proteins after infection,and procollagen Ⅱ mRNA in cells was detected increasingly by Northern blot.TGF-?1 stimulated proliferation of chondrocytes in primary cultured,and resulted in recruitment of articular chondrocytes into S-G2/M phase.Chondrocytes seeded onto BMG showed high level of proliferation.Transplantation in vivo showed: cartilage defect was repaired satisfactorily by Combination of BMG and infected chondrocytes,and the structure of repair tissue got close to normal articular cartilage.[Conclusion] Articular chondrocytes prolifera tion could be stimulated by adenovirus vector taking TGF-?1 genes,which could be used to constructed tissue-engineered cartilage,and applied in repairing joint cartilage defect.

Adult ; Antihypertensive Agents ; therapeutic use ; Blood Pressure ; drug effects ; Depression ; complications ; drug therapy ; physiopathology ; Drug Therapy, Combination ; Drugs, Chinese Herbal ; therapeutic use ; Female ; Humans ; Hypertension ; complications ; drug therapy ; physiopathology ; Male ; Middle Aged ; Phytotherapy ; Powders ; Treatment Outcome