Objective To investigate the feasibility of searching for proteins which interact with intracellular domain of hematopoietic growth factor receptors using yeast two-hybrid system. Methods RT-PCR method was performed to amplify the genes of intracellular domains of G-CSF receptor and EPO receptor in NFS-60 and BET-2 cells of mice. The genes were cloned into yeast expression plasmid pGBKT7 vector,and then transformed into yeast AH109. The yeast proteins were isolated and analyzed with Western blotting. Transcriptional activation was analyzed by the ?-galactosidase colony-lift filter assay. Results The intracellular domains of G-CSF receptor and EPO receptor genes were successfully cloned into pGBKT7 vector. The results of Western blotting assay showed that both proteins were expressed in the yeast cells. The ?-galactosidase colony-lift filter assay demonstrated that G-CSF receptor alone had no activity of transcriptional activation,while the EPO receptor alone could activate transcription. Conclusion The findings suggested that intracellular domain of G-CSF receptor could be used as a bait to find interacting proteins using yeast two-hybrid system,while that of the EPO receptor could not. Therefore the system could not be applied to all hematopoietic factor receptor.

To measure the relative affinity and type Specificity of neutralizing monoclonal antibodies against the hexon antigen of adenovirus type 7,a simple ELISA double antibody binding system has been developed.By this method,ralative affinity of nine MAbs was estimated from the antibody concentrations at approximately 50% of plateau binding and were ranked.The theoretical basis for this method was discussed. Hybridomas secreting antibodies of desired affinity can be selected at an early stage after fussion by measuring relative affinity of hybridomas supernatants.The type specificity was judged by the difference between end-point concentration against adeno- virus type 7 and type 3.And the relation between affinity and specificity of MAbs was discussed.

Objective To study the value of MRI in the diagnosis of spinal metastasis.Methods MR imaging manifestations of spinal metastasis in 85 cases were retrospectively analyzed.46 cases underwent CT and/or X-ray in the 85 cases.Results Totally 388 metastatic vertebra in the 85 cases were detected by MRI.388 vertebras showed hypointensity on T1WI.354 vertebra showed mixed signal intensity and 34 vertebra showed hypointensity on T2WI.All of the vertebra showed hyperintensity on T2WI/STIR.54 vertebra showed pathologic fracture,53 vertebra were protrusion in postetrior,54 vertebra with appendages damage and paravertebral soft tissue mass presented and vertebral canal involved in 30 cases.18 cases were detected with lesion in 46 cases having X-ray and/or CT data,the positive rate was 39.11%(18/46).Conclusion MRI can detect spinal metastatic lesion early,the extent and degree of the tumors can be judged by MRI exactly.

Oncology is extensive in contents,covering a wide range of organs,systems and clinical specialties.Here,we discuss the feasibility and necessity of flipping the classroom teaching through the introduction of Oncology and through the implementation of flip classroom teaching for oncology graduate students,this paper analyzes the evaluation of the classroom teaching by the teachers and students,and compares the assessment results of students under different teaching methods.The results show that the flipped class can promote students' initiative learning,promote students' classroom participation,and help students to internalize and consolidate their knowledge of oncology in the theoretical teaching of graduate oncology.

Objective To investigate the effect of over-expression of human ribonuclease inhibitor suppresses invasion and migration of transplanted bladder cancer .Methods The T24 cells were stably transfected with pIRES2-EGFP-RI and pIRES2-EG-FP plasmid respectively .Using the cell transfected with pIRES2-EGFP and untransfected cell as controls .and the positive clones were screened by G418 ,respectively ;Tumor cells of the three groups at 2 × 106 were respectively injected into the back of BALB/C nude mice to establish the xenograft models .Change of micro-blood vessels in tumor tissue and expression of CD31 were detected by Immunohisto-chemical and HE staining .Immunohisto-chemical assay was used to detect the expression of RI ,MMP-2 ,MMP-9 ,E-cadherin ,N-cadherin ,Vimentin ,Snail ,Slug ,Twist in the tumors .Results Animal experiment showed that the T24-RI cells group significantly inhibited the growth of bladder cancer compared with the other two control groups .Compared with the T24 and T24 vector cells groups ,the microvessel density in tumor tissue of T24-RI group was notably reduced and the expressions of MMP-2 , MMP-9 ,N-cadherin ,Vimentin ,Snail ,Slug ,Twist significantly were decreased simultaneously ,while the expressions of RI and E-cadherin were increased .Conclusion up-regulation RI can inhibit the growth of transplanted bladder cancer in nude mice by decrea-sing the expression of invasion protein and EM T protein .

Aim To investigate the role of calcitonin gene related peptide(CGRP),ATP sensitive potassium channel(KATP)and spinal nerve in the protective effect of intrathecal morphine preconditioning against myocardial postischemia injury in rats.Methods Rats were established intrathecal catheter placement and myocardial ischemia reperfusion models were randomly assigned to 10 groups:control group(CON);intravenous dimethylsulfoxide group(DMSO);intravenous CGRP8-37 group(CGRP8-37,a selective CGRP receptor antagonist,3 nmol?kg~-1);intravenous glibenclamide group(GLI,a non-selective KATP channel inhibitor,0.3 mg?kg~-1);intrathecal 10 ?l of 1% lidocaine group(LID);intrathecal 3?1 ?g?kg~-1 morphine preconditioning group(MPC);intravenous 3 nmol?kg~-1 CGRP8-37+MPC group(CGRP8-37+MPC);intravenous 0.3 mg?kg~-1 GLI+MPC group(GLI+MPC);intravenous 0.3 mg?kg~-1 GLI+LID group(GLI+LID).Indicators to be observed were MAP,HR and RPP(MAP?HR),the volume of area at risk(AAR)and infarct size(IS),and the area of myocardial infarction,which was demonstrated by IS/AAR.Results Compared to CON group,the volume of IS and IS/AAR was reduced in MPC,LID,LID+MPC and GLI+LID group(P

Wether cancer patients can re-adapt to life or not after experiencing tremendous frustration is the focus of tumor care nowadays.Since the measurement methods and outcome indexes of cancer patients adaptation level are different,adaptation related factors and predictors are controversial and there is not yet a unified opinion.Currently,it is thought that demographic information (like age,gender,education),disease-related information (like physiological indicators,treatment options,body image and sex) are closely related to cancer patients adaptation after stress.However there are huge differences among the extents and effects.

Objective To investigate the relationship between the activity of Janus kinase/signal transducer and activator of transcription (JAK/STAT) signaling pathway in cerulein-induced pancreatic acinar cell line AR42J. Methods The in vivo model of AP was induced by cerulean treated pancreatic acinar cell line AR42J, then RPM and AG490 were given for intervention. Western blot was used to determine theexpressions of JAK1 and phosphorylation JAK1 ( P JAK1 ) , STAT1, PSTAT1 and TNFα, IL-1β, IL-6. The expressions of IL-6, IL-1 β, and TNFα mRNA were measured by RT-PCR. Survival rate of cells was evaluated by trypan blue stain. Results The relative expressions of JAK1, P JAK1, STAT1, P STAT1 and TNF-o, IL-1β, IL 6 without cerulean treatment were 0.09 ±0.04,0.14 ±0.08,0.21 ±0.09,0.12 ±0.12,0.10 ±0.02,0.08 ± 0.03,0.02 ± 0.02. After cerulean treatment, the expressions of abovementioned protein increased in a time-dependant manner, the expressions at 24h were 0.53 ± 0.09,0.53 ± 0.13,0.56 ± 0.09,0.55 ± 0.10,0.25 ± 0.04,0.25 ±0.09,0.27 ±0.07, which were significantly higher than those in the control group (P ＜0.05). 2 4 h after RPM and AG 4 9 0 inhibition, the expressions of TNF-α, IL-1 β, IL-6 proteins significantly decreased to 0.17 ± 0.03and 0.17 ± 0.01,0.15 ± 0.05 and 0.14 ± 0.07,0.19 ± 0.04 and 0.19 ± 0.05; their expressions of mRNA significantly decreased ( P ＜ 0.05 ). The cell survival rates in RPM and AG490 treatment group were (72.4 ± 11.2) %, (69.7 ± 9.8 ) %, and in cerulein-stimulated cells (42.2 ± 12.3 ) % ( P ＜ 0.05 ).Conclusions The JAK1/STAT1 signaling pathway was involved in pancreatic inflammatory response with cerulein stimulation. Early treatment with inhibitors to the JAK1/STAT1 signaling pathway might control the inflammatory response in acute pancreatitis.

A medical studies have indicated that there would be a defense response from human body after TBI,which mainly comes with inflammatory reaction. Human body's resistance seems likely to be enhanced,during this process, by inflammatory cytokines leading to rehabilitation of the cellular organization.However,a strong Inflammation from the whole human body may be caused due to the excessive activation,which, instead, worsens secondary brain injury. This article discusses the relationship between cytokines and traumatic brain injury.

Objective To investigate the effect of electro-acupuncture at Guanyuanshu, Xinshu, Neiguan points on cardiac function after acute myocardial ischemia in rabbits. Methods A total of 36 rabbits were randomly divided into a control group, a model group and a treatment group. Acute myocardial ischemia was induced with Jugular vein injection of pituitrin. At 10 min before modeling, 1 hour and 1 week after modeling, the levels of malondialdehyde (MDA) and creatine kinase(CK) were determined respectively, the cardiac function was assessed as the maximum rate of left ventricular pressure rise(dp/dtmax)and the peak left ventricular pressure (LVSP). Results After 1 week of treatment, the serum levels of CK and MDA in the treatment group reduced significantly(120.21±10.67 U/L, 21.35±2.04 mmol/L], compared to the model group (162.37±13.21 U/L, 30.13±2.75 mmol/L) (all P<0.05);the dp/dtmax and LVSP decreased percentage in the treatment group (20%±2.43%, 7.22%±0.97%) decrease significantly compared to the model group (31.5%± 4.14%, 12.19%±2.21%) (all P<0.05). Conclusion Electro-acupuncture of Guanyuanshu, Xinshu, Neiguan acupoints can significantly improve cardiac function after acute myocardial ischemia and protect against acute myocardial ischemia in rabbits.