1.The study of malignant transformation of NIH3T3 cells induced by transfection of HMGA1 gene
Xin ZHANG ; Mingguang ZHU ; Hongyan YUAN ; Wei YANG ; Yaping CHANG
Chinese Journal of Immunology 1985;0(05):-
Objective:To establish a mouse fibroblastic cell line stably transfected with HMGA1 gene,and to use the cell line to investigate the role of HMGA1 in tumor development and progression.Methods:Eukaryotic expression vector pcDNA3.0-HMGA1 was transfected into NIH3T3 by lipofectamine-mediation.Stable transfectants were selected by G418.The expression of extraneous HMGA1 gene was analyzed by RT-PCR and DNA sequencing.Cell growth was measured through MTT and the cell cycle by FCM.Soft agar colony formation was employed to analyze the ability of anchorage-independent growth.The expression of the immune inhibition factors of VEGF and FasL mRNA was detected by RT-PCR.Results:NIH3T3 cell lines stably expressing HMGA1 gene were successfully established.Comparing with controls,the HMGA1 gene-transfected cells grew faster,acquired the ability of anchorage-independent growth and expressed the immune inhibition factors of VEGF and FasL mRNA.Conclusion:Extraneous expression of HMGA1 gene can induce malignant transformation of NIH3T3 and modulate mRNA expression of immune inhibition factors.HMGA1 gene plays a key role in tumor development,progression and immune escape.
2.CT and MRI in the comprehensive diagnosis of intrahepatic peripheral cholangiocarcinoma
Bin QIN ; Cuiping YUAN ; Youdan LIN ; Xin ZHU
Journal of Practical Radiology 2015;(10):1624-1627
Objective To analyze the value of CT and MRI in the diagnosis of intrahepatic peripheral cholangiocarcinoma (IHPCC ). Methods 32 cases of intrahepatic peripheral cholangiocarcinoma were collected and analyzed retrospectively.25 patients underwent plain and enhanced CT scan,while 18 cases underwent plain MRI and MRCP(8 of them underwent MRI dynamic enhancement scanning).6 cases underwent plain and enhanced scan of CT and MRI simultaneously.Results 32 intrahepatic lesions were found,with CT and MRI revealing all lesions.Lesions of IHPCC showed as hypo-or iso-density on the plain CT,slightly hypo-intensity on T1 WI and uneven mild hyper-intensity on T2 WI.During arterial phase of contrast-enhanced scan,18 lesions showed as marginal mild linear enhancement,3 cases with obvious enhancement and 11 cases with no enhancement.During portal phase,venous phase and delayed scan,19 lesions showed as progressive mild-to-mod-erate uneven enhancement,while 6 cases enhanced homogeneously.25 cases showed characteristic significantly delayed enhance-ment.MRCP could display satisfactory of Intra-and-Extra-hepatic bile ducts.Other signs include intrahepatic bile ducts dilation (23 cases),intrahepatic bile duct stones (6 cases),hepatic lobe atrophy (1 5 cases),and depressed liver capsula (8 cases).Conclusion Both CT and MRI are effective methods for diagnosing of intrahepatic peripheral cholangiocarcinoma.Compared with CT,MRI seems more valuable on presentation the tumor size and border,bile duct involvement,the degree of expansion and portal vein inva-sion,etc.It is more valuable to combine CT with MRI for the diagnosis of intrahepatic peripheral cholangiocarcinoma.
3.Effects and its mechanisms of hypertonic saline hydroxyethyl starch 200/0.5 solution in rats with ischemic cerebral edema
Xiangdong YUAN ; Wenqiang JIANG ; Xin JIANG ; Gaofeng ZHU ; Hongke ZENG
Chinese Journal of Emergency Medicine 2015;24(3):299-303
Objective To investigate effects and its mechanisms of hypertonic saline hydroxyethyl starch 200/0.5 solution on intracranial pressure and brain water content in rats with ischemic cerebral edema.Methods All experiments were conducted in the animal experimental center of Sun Yat-sen University.The 28 male Sprague-Dawle (SD) rats were randomly (random number) divided into hypertonic saline hydroxyethyl starch group,hydroxyethyl starch group,control group and sham operation group,each n =7.Ischemic cerebral edema model was reproduced by middle cerebral artery occlusion (MCAO),followed by reperfusion after ischemia for 2 hours (If the moldel was not successful,other rats were operated to fill the missing models).Then reperfusion after ischemia 2 hours and received hypertonic saline hydroxyethyl starch and hydroxyethyl starch via tail vein at the beginning of reperfusion.The colloidal osmotic pressure (COP) and intracranial pressure (ICP) were evaluated on 0,2,6,12,18,24 hours after the surgery.The water content of the right hemisphere was measured on 24 h after the surgery.Results The ICP of hypertonic saline hydroxyethyl starch group,hydroxyethyl starch group and control group were significantly higher than that of sham operation group on 2,6,12,18,24 h after the surgery.The ICP of hypertonic saline hydroxyethyl starch group was significantly lower than those of hydroxyethyl starch group and control group on 2,6,12,18 and 24 h.But there was no significant difference in ICP of the hydroxyethyl starch group compared with that of control group at all time points.The COP of hypertonic saline hydroxyethyl starch group and hydroxyethyl starch group were significantly higher than the control group and sham operation group at each time point; There was no significant difference in COP (mmHg) of the hydroxyethyl starch group compared with that of hypertonic saline hydroxyethyl starch group at all time points.The brain water content (BWC) of hypertonic saline hydroxyethyl starch group,hydroxyethyl starch group and control group were significantly higher than that of sham operation group on 24 hours after the surgery [(81.24±0.36)%,(83.04±0.10)%,(83.14±0.41)% vs.(78.37±0.37)%,all P=0.000],BWC of hypertonic saline hydroxyethyl starch group lower than these of hydroxyethyl starch group [(81.24±0.36)% vs.(83.04 ±0.10) %,P =0.000] and control group [(81.24 ±0.36)% vs.(83.14 ±0.41) %,P =0.000].There was no significant difference in BWC of the hydroxyethyl starch group compared with that of control group [(83.04 ± 0.10) % vs.(83.14 ± 0.41) %,P =0.578].Conclusion Hypertonic saline hydroxyethyl starch solution could significantly ameliorate ischemic cerebral edema and reduce ICP,but the relationship between its elevated COP and reduced ICP has not been confirmed.
4.A commentary on a case of aplastic anemia applying for identification of occupational chronic severe benzene poisoning.
Hong-ping DENG ; Shi-xin ZHU ; Jian-yuan CAI
Chinese Journal of Industrial Hygiene and Occupational Diseases 2012;30(9):707-708
Anemia, Aplastic
;
diagnosis
;
etiology
;
Benzene
;
poisoning
;
Expert Testimony
;
Humans
;
Male
;
Occupational Diseases
;
diagnosis
5.First line gefitinib monotherapy for brain metastases patients with advanced non-small cell lung cancer
Zhu WANG ; Jiandong TONG ; Xin YUAN ; Fang HAN
Chinese Journal of Primary Medicine and Pharmacy 2008;15(9):1419-1420
Objective To evaluate the efficacy and toxicity of gefitinib on the treatment of advanced NSCLC with brain metastases.Methods 17 cases received gefitinib 250 mg/m2/d,30 days a cycle.Results Objective response rate of 35.3% was achieved,and DCR was 70.6% ,the toxic reactions could be controlled with relative therapy.Conclusion Gefitinib is effective and well tolerable for advanced NSCLC with brain metastases.It is worth to be used.
6.Neoplasty of multiple cerebrospinal fluid rhinorrhea on combined frontal-nose approach through endoscope.
Wei-Yuan SUN ; Fu-Ming ZHU ; Xin-Hua XU
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2008;43(6):460-462
Cerebrospinal Fluid Rhinorrhea
;
surgery
;
Craniotomy
;
methods
;
Endoscopy
;
Frontal Sinus
;
surgery
;
Humans
;
Male
;
Nose
;
surgery
;
Reconstructive Surgical Procedures
;
methods
;
Young Adult
7.Efficacy on dysphagia induced by bulbar palsy treated with acupuncture at Tiantu (CV 22) with different needling techniques.
Yuan ZHU ; Li-xin FU ; Xue-min SHI
Chinese Acupuncture & Moxibustion 2014;34(11):1089-1092
OBJECTIVETo compare the efficacy between elongated needle therapy and regular needle therapy at Tiantu (CV 22) on the basis of xingnao kaiqiao (activiting brain and regaining consciousness) acupuncture therapy so as to explore the effective therapeutic method in treatment of dysphagia induced by bulbar palsy.
METHODSSeventy one cases of dysphagia induced by bulbar palsy were randomized into two groups. The xingnao kaiqiao acupuncture therapy was applied at Shuigou (GV 26), Neiguan (PC 6), Sanyinjiao (SP 6) and the others in the two groups. In the elongated needle therapy group, on the basis of xingnao kaiqiao acupuncture therapy, the elongated needle was used to puncture Tiantu (CV 22). In the regular needle therapy, the regular acupuncture technique was used at Tiantu (CV 22). In both groups, the treatment was given once a day in a week except Sunday and lasted for 4 weeks totally. Before and after treatment, the swallowing condition and the standardized swallowing assessment (SSA) were observed in the patients and the efficacy was compared between the two groups.
RESULTSThe total effective rate was 97.2% (35/36) in the elongated needle therapy group, which was better than 77.1% (27/35) in the regular needle therapy group (P<0.05). After treatment, SSA score was reduced significantly as compared with that before treatment in the two groups (both P<0.05). SSA score in the elongated needle therapy group was reduced much more apparently as compared with that in the regular needle therapy group after treatment (P<0.05).
CONCLUSIONOn the basis of the xingnao kaiqiao acupuncture therapy, the elongated needle therapy at Tiantu (CV 22) achieves the superior effect on bulbar palsy-induced dysphagia as compared with the regular acu- puncture at Tiantu (CV 22).
Acupuncture Points ; Acupuncture Therapy ; Aged ; Bulbar Palsy, Progressive ; complications ; Deglutition Disorders ; etiology ; therapy ; Female ; Humans ; Male ; Middle Aged ; Treatment Outcome
8.Effects of HMGA1 silence by RNA interference on the apoptosis and cell cycle of hepatocarcinoma cells
Mingguang ZHU ; Xin ZHANG ; Hongyan YUAN ; Wei YANG ; Yaping CHANG
Chinese Journal of Immunology 2001;0(07):-
Objective:To develop HMGA1-silencing SMMC-7721 cell and investigate its impacts on apoptosis and cell cycle in tumor cells.Methods:Constructing HMGA1-silencing SMMC-7721 cell through G418 selection of RNAi plasmid transfected cells;surveying the apoptosis,prliferation and cell cycle of the tumor cells by FACS and MTT.Results:Stable HMGA1-silencing cells were obtained successfully.The apoptosis percentage in RNAi group(29.46?3.04%) was significantly higher than that in the negative control (1.96?0.76%) or control (2.04?0.70%)within each P
9.Linkage disequilibrium and mutation rate analysis of sixteen X-STR loci.
Li LI ; Jun-hong LIU ; Ru-xin ZHU ; Yuan LIN
Journal of Forensic Medicine 2014;30(6):437-440
OBJECTIVE:
To assess the patterns of linkage disequilibrium (LD) of 16 STR loci on X chromo- some and investigate the genetic stability.
METHODS:
Genomic DNA samples extracted from blood stains from 500 unrelated individuals and 885 lineage members from Eastern Chinese Han population were genotyped through multiplex amplification using IDtyperX-16 kit by our independent research followed by capillary electrophoresis. LD was assessed by PowerMarker v3.25 software and mutation rate of every locus was analyzed.
RESULTS:
LD were not found at the 16 X-STR loci. Allele mutations were observed at 10 loci. Among them, mutation rates of DXS6809 and DXS7132 were both up to 0.0048.
CONCLUSION
When the 16 X-STR loci included in IDtyperX-16 kit were used for parentage testing, product princi- ples can be applied to calculate the likelihood, but mutation should be taken into consideration in the case that the genotypes do not meet the genetic law (especially at DXS6809 and DXS7132).
Alleles
;
Asian People/genetics*
;
Blood Stains
;
China
;
Chromosomes, Human, X/genetics*
;
Electrophoresis, Capillary
;
Female
;
Forensic Genetics/methods*
;
Gene Frequency
;
Genetic Loci/genetics*
;
Genotype
;
Humans
;
Linkage Disequilibrium/genetics*
;
Microsatellite Repeats/genetics*
;
Multiplex Polymerase Chain Reaction
;
Mutation
;
Mutation Rate
10.Study on the water-soluble composition release of compound Danshen sustained-release tablet.
Shen-shang ZHU ; Xu-jiang YUAN ; Yuan-xin LI
China Journal of Chinese Materia Medica 2006;31(1):37-40
OBJECTIVETo study on the release of compound Danshen sustained-release tablet and the evaluate method of Chinese material medica compound sustained-release preparation.
METHODRotating basket method and HPLC were employed.
RESULTThrough the determination of 6 time-point samples, the water-soluble compositions of compound Danshen sustained-release tablet had a Well-balanced release behavior with a zero-grade release model or Higuchi release model.
CONCLUSIONCompound Danshen sustained-release tablet had a zero-grade release model. The method was rapid and stable and could be applied to evaluate the water-soluble composition release of compound Danshen sustained-release Tablet.
Benzofurans ; analysis ; Chromatography, High Pressure Liquid ; Delayed-Action Preparations ; Drug Combinations ; Drug Stability ; Drugs, Chinese Herbal ; administration & dosage ; chemistry ; isolation & purification ; Ginsenosides ; analysis ; Panax ; chemistry ; Plants, Medicinal ; chemistry ; Salvia miltiorrhiza ; chemistry ; Solubility ; Tablets