OBJECTIVE:To study the liver damage mechanism in mice caused by the incompatibility of Daphne genkwa com-bined with Glycyrrhiza uralensis in aspect of liver transporter. METHODS:40 mice were equally randomized into a normal control (isometric normal saline)group,a group of G. uralensis [15 g(medicinal matierias)/kg],a group of D. genkwa [15 g(medicinal tatierias)/kg],a group of D. genkwa with G. uralensis in the ratio of 1∶1 [15 g(crude drug)/kg],a group of D. genkwa with G. uralensis in the ratio of 1∶3 [15 g(medicinal matierias)/kg](n=8). The mice were given the corresponding drug,ig,once a day for 7 consecutive days. HE staining was performed and then the pathomorphology of liver tissues were observed under the light mi-croscope,and calculation was made for pathological grading. Western blot method was employed to determine the protein expre-ssion of the transporter Ntcp protein in the livers of mice. The contents of total bile acids(TBA)in livers of mice were determined. RESULTS:Compared to the mice in the normal control group,those in the group of 1∶1 and 1∶3 demonstrated higher protein ex-pression of Ntcp. In the group of 1∶1,the mice with grade“+++”hepatocyte degeneration were more (8). The mice with grade“+++”and“++”hepatocyte degeneration in the groups of 1∶3 were more (2 and 8 respectively). CONCLUSIONS:D. genkwa combined with G. uralensis can induce liver damage in mice by a mechanism which may be related to the accumulation of a large amount of TBA in the liver as a result of the increase in the expression of Ntcp in mice.

OBJECTIVE To study the diagnosis and treatment of parotid acinic cell carcinoma. METHODS The clinical data of 19 cases with parotid acinic cell carcinoma treated from 1985 to 2007 were retrospectively reviewed.Four patients underwent superficial parotidectomy,5 patients underwent total parotidectomy,6 patients underwent superficial parotidectomy plus partial deep parotidectomy,6 patients underwent total parotidectomy and neck dissection.Eight patients received postoperative radiotherapy.One patient only received radical radiotherapy after biopsy.All patients were followed up for 1 to 20 years.RESULTS Nobody died after operation.Five cases recurred after operation, and the recurrence rate was 26.3%(5/19) CONCLUSION The main treatment for parotid acinic cell carcinoma is complete resection and postoperative radiotherapy.

OBJECTIVE:To prepare and appraise eucalyptus oil?-cyclodextrin inclusion compound,and to testify the feasibility of transforming the dosage form of eucalyptus oil by clathration techniques.METHODS:The physicochemical properties of inclusion compound was identified by thin-layer chromatography(TLC),infrared spectroscopy(IR),ultra-violet spectroscopy(UV)and gas chromatograph mass spectrometer(GC-MS),respectively.Meanwhile,the changes in constituents and clathration outcomes of eucalyptus oil before and after clathration were also investigated.RESULTS:The analytic result of TLC,IR and UV showed that stable inclusion compound has been formed from eucalyptus oil and?-cy?clodextrin.The result of GC-MS demonstrated that there was no significant change in the essential components and the percentage composition of eucalyptus oil before and after inclusion.CONCLUSION:Stable inclusion compound can be made from eucalyptus oil and?-cyclodextrin meanwhile without changes in main components and the percentage composition of eucalyptus oil.

China ; Developed Countries ; Insurance, Health ; Occupational Health

Objective To investigate the effect of anti thymic globulin (ATG)combined with cyclosporine (CSA)immunosuppressive therapy in patients with severe aplastic anemia (SAA).Methods 20 patients with SAA were treated with CSA combined with immunosuppressive therapy,and the changes of blood indexes after treatment were observed.Results After treatment,4 cases (13.33%)were cured,11 cases (36.67%)were relieved,8 cases (26.67%)were improved,7 cases (23.33%)were ineffective,the total effective rate was 76.67%.After the treatment of blood routine,examination of Hb,WBC and PLT treatment 6 months compared with before treatment,the difference were statistically significant (t =5.678,3.675,7.685,all P <0.05).Patients after treatment,1 case had allergic reaction,which accounted for 3.33%,and was finally given the glucocorticoid,then improved.1 case had extremely low WBC,accounting for 3.33%,appeared serious infection and death after testing.Conclusion The effect of ATG combined with CSA immunosuppression in the treatment of SAA is effective obviously,and it has a certain safety,vwhich is worthy of clinical recommendation.

Background and purpose:There were reports that radiotherapy might intensify angiogenesis and result in the resistance of tumor to radiotherapy.Endostar,a new type rh-endostatin,may specifically inhibit angiogenesis and tumor growth,so it may reduce the resistance to radiotherapy.This study was to evaluate the inhibitory effect of Endostar in combination with radiation compared with cisplatin(DDP) plus radiation on lung adenocarcinoma A549 in BALB/c nude mice.Methods:We established A549 lung adenocarcinoma xenograft models.When the largest diameter of tumor reached 1.0 cm,all mice were randomized into 4 groups(n=6):control,radiation alone,radiation plus DDP,radiation plus Endostar.We measured the largest diameter and the vertical diameter of the tumors at different time points after treatment.At the 16th day,mice were executed and the tumors were subjected to removal for the analysis of apoptosis,and the expression level of basic fibroblast growth factor(bFGF) mRNA by reverse transcription-polymerase chain reaction(RT-PCR) and vascular endothelial growth factor(VEGF) were also detected by immunohistochemistry.Results:The tumor growth velocity of control,radiation alone,radiation plus DDP,radiation plus Endostar were(162?6)%,(131?8)%,(104?7)% and(108?11)% respectively(P0.05).The apoptosis rates of control,radiation alone,radiation plus DDP,radiation plus Endostar were(12.2?1.1)%,(16.5?0.8)%,(24.4?1.5)% and(20.5?1.9)%,respectively(P

Objective To obtain the recombinant urease B subunit (ureB) of Helicobacter pylori (Hp) and apply it in serological detection of Hp infected patients. Methods Urease B subunit gene was amplified from the complete genome of Helicobacter pylori by PCR, and cloned into the PinPoint TM Xa Ⅲ fusion expression vector, then was sequenced. The protein of urease B subunit was expressed in E.coli JM109 and purified with affinity chromatography. 113 serum samples of peptic ulcer patients were detected by ELISA combined with purified UreB protein. Results DNA sequence analysis showed the nucleic acid sequence homology of ureB gene was 96.44% and the putative amino acid sequence homology was 99.65%. The recombinant UreB protein was composed of 571 amino acid residues and kept original immunologic reaction with corresponding antibody, and its purity was over 90% after affinity chromatography. The results of ELISA associated with recombination UreB antigen showed the sensitivity and specificity was 92%, 98.5%. Conclusion The recombinant UreB protein will be of value for clinical serodiagnosis and epidemiological study of Helicobacter pylori.