1.Relationship of epigenetic and Dao-di herbs.
Yuan YUAN ; Yuan WEI ; Jun YU ; Lu-qi HUANG
China Journal of Chinese Materia Medica 2015;40(13):2679-2683
Dao-di Herbs is specificity and locality, and its unique phenotypic features is closely related to the growth and development of medicinal plants. In addition to traditional genetic, epigenetic play an important role in formation of Dao-di herbs. This paper introduces the concept of epigenetic and the role of DNA methylation in the gene expression regulation. We further prospects epigenetic mechanism in study of Dao-di herbs formation from specific phenotype and regional analysis. And study on the relationship of epigenetic and Dao-di herbs will provide a basis for quality assessment and identification of Chinese drugs.
DNA Methylation
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Drugs, Chinese Herbal
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standards
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Epigenesis, Genetic
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Gene Expression Regulation, Plant
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Plants, Medicinal
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genetics
2.Evaluation of Residues of Chrysanthemum Ester Pesticides and Heavy Metals in Oolong Tea Samples
Wei HUANG ; Guojian LI ; Dongxing YUAN
Journal of Environment and Health 1992;0(02):-
Objective To analyze the residues of chrysanthemum ester pesticides and heavy metals in several typical Oolong tea samples for evaluating the drink safety. Methods The residues of chrysanthemum ester pesticides in the tea samples were extracted with ultrasonic method. Gas chromatography equipped with ECD was applied to determine the pesticide residues. The heavy metals in tea samples were analyzed with an inductively coupled plasma-mass spectrometer (ICP-Mass) and an atomic fluorescence spectrometer. Results The residues of chrysanthemum ester pesticides in the dry tea samples were 0.200-0.780 mg/kg, no residues was detected in the tea water. The content of heavy metals in the dry tea and tea water was very low, under the standard limits. Conclusion The Oolong tea samples chosen in the present investigation have not been polluted by chrysanthemum ester pesticides and heavy metals, according to the results, the Oolong tea made in the area is safe for drink.
3.Epidemiological investigation and molecular etiology of Hand-Foot-Mouth Disease of children in Zhong-shan city from 2011 to 2015
Sang HUANG ; Chunlei YUAN ; Huixiong WEI
The Journal of Practical Medicine 2017;33(11):1861-1864
Objective To study the epidemiological characteristics of Hand-Foot-Mouth Disease of chil-dren in Zhongshancity from 2011 to 2015 and provide etiological evident for the prevention and control of Hand -Foot -Mouth Disease of children in Zhongshan city. Methods We analyzed the data of children with Hand-Foot-Mouth Disease in Zhongshan Boai 's Hospital from 2011 to 2015. The enterovirus nucleic acid of Hand-Foot-Mouth Diseaseof children were detected. Results Atotal of 31164 cases of Hand-Foot-Mouth Disease were diagnosed in Zhongshan Boai′s Hospital during 2011-2015,the ratio of male to female was 1.62:1. Every year,the peak inci-dence of the disease is not the same. During 2011-2015,there were 10147 cases of Hand-Foot-Mouth Diseaseof children,among which,through viral nucleic acid EV71and CoxA16testing,2062 cases were positive and the positve rate was 20.32%. Pathogen of Hand-Foot-Mouth Diseasewas mainly based on EV71 in 2011,2012, 2013and2015,accounting for the total positive rate of 79.04%,72.46%,81.60%,73.73%respectively;Pathogen of Hand -Foot -Mouth Diseasewas mainly based on Cox16 in 2014,accounted for 54.47% of the total positive. In different years EV71 and CoxA16 positive rate was significant different.There were 1982 cases repeated Hand-Foot-MouthDiseaseinfectioins .Conclusion The incidence of Hand-Foot-Mouth Disease of children is related to age , sex and season in Zhongshan city. It is mainly based on clinical diagnosis ,the preventive measures and the clinical recognition in time to provide help for the treatment and control of Hand-Foot-Mouth Disease of children.
4.Paratesticular desmoplastic small round cell tumor: report of a case.
Yuan HUANG ; Wei-qiang HUANG ; Wei-bo MAO ; Wei GONG ; Cheng-feng GU
Chinese Journal of Pathology 2009;38(8):559-560
Anion Exchange Protein 1, Erythrocyte
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metabolism
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CD56 Antigen
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metabolism
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Desmin
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metabolism
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Desmoplastic Small Round Cell Tumor
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metabolism
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pathology
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surgery
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Diagnosis, Differential
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Humans
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Lymphoma
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metabolism
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pathology
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Male
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Middle Aged
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Rhabdomyosarcoma
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metabolism
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pathology
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Sarcoma, Ewing
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metabolism
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pathology
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Testicular Neoplasms
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metabolism
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pathology
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surgery
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Vimentin
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metabolism
5.Effects of bifidobacterium on mRNA expression of tumor necrosis factor receptor-associated factor 6, glycogen synthase kinase-3β and miRNA-146a in intestinal epithelial cells induced by lipopolysaccharide in rats
Yuan YUAN ; Wei ZHOU ; Jing LI ; Weiming YUAN ; Longguang HUANG ; Shaowei ZHENG
Chinese Journal of Applied Clinical Pediatrics 2015;30(2):110-113
Objective To detect the effects of bifidobacterium or bifidobacterium cultured supernatant on the mRNA expression of tumor necrosis factor receptor-associated factor 6 (TRAF6),glycogen synthase kinase-3β (GSK-3β) and the miRNA-146a in rat small intestinal epithelial cell(IEC-6) induced by lipopolysaccharide (LPS).Methods IEC-6 in logarithmic phase were randomly divided into LPS group,cultured supernatant group and inactivated bacteria group.All the 3 groups were exposed to 5 mg/L LPS for 5 hours,and then 1 mL sterile saline was added in LPS group and culturing continued for 24 hours ; 1 mL bifidobacterium cultured supernatant was added in cultured supernatant group and culturing continued for 24 hours;1 mL inactivated bifidobacterium 1 x 1010 CFU/L added in inactivated bacteria group and continued culturing for 24 hours.The mRNA expressions of TRAF6,GSK-3 β and miRNA-146a were detected by reverse transcription-polymerase chain reaction (RT-PCR).Results The level of TRAF6,GSK-3 β of culture supematant group (0.72 ± 0.05,0.46 ± 0.14) were all lower than LPS group (1.01 ± 0.14,1.02 ± 0.25),but the level of miRNA-146a(3.05 ± 0.40) was higher than that in LPS group(1.01 ± 0.12),and there were significant differences between them (t =5.278,6.316,13.218,P =0.000).The level of GSK-3 β of inactivated bacteria group(0.59 ±0.13) was significantly lower than that in LPS group(t =4.837,P =0.000).The levels of TRAF6 and miRNA-146a of inactivated bacteria group(1.05 ±0.11,0.78 ±0.22) had no significant differences with LPS group (t =0.732,1.463,P > 0.05).The level of TRAF6 of cultured supernatant group was lower than that in inactivated bacteria group,and the level of miRNA-146a was higher than that in inactivated bacteria group,and there were significant differences between 2 groups (t =6.009,14.687,P =0.000).Conclusions Bifidobacterium cultured supernatant and inactivated bacteria both have certain protective effect on the IEC-6 induced by LPS.One of the protective mechanisms of bifidobacterium cultured supernatant may be achieved by elevating the expression of miRNA-146a,and decreasing the levels of inflammation related factor TRAF6 and damage related factor GSK-3β.The protective effects of inactivated bifidobacterium may be achieved by decreasing the level of damage related factor GSK-3β.
6.Acute toluene or xylene poisoning case analysis of domestic journals.
Jian-shu HUANG ; Yuan-ling ZHOU ; Wei-guo WAN
Chinese Journal of Industrial Hygiene and Occupational Diseases 2013;31(5):369-371
Acute Disease
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Adolescent
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Adult
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Aged
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Female
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Humans
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Male
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Middle Aged
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Toluene
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poisoning
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Xylenes
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poisoning
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Young Adult
7.Clinical features of children infected with influenza virus A in year 2009 in Wuhan area
Cong WEI ; Yuan HUANG ; Kun ZHENG ; Dongchi ZHAO
Chinese Journal of Applied Clinical Pediatrics 2014;29(22):1696-1699
Objective To explore the clinical and pandemic features and respiratory pathogen co-infection characteristics of children infected with influenza virus A (Flu A) in year 2009.Methods A retrospective study of hospitalized children with flu-like clinical symptoms in 2009 was conducted.The peripheral blood serum was analyzed to detect common respiratory agents,inclucding Flu A,respiratory syncytial virus,adenovirus,influenza virus B,parainfluenza virus 1,2 and 3,chlamydia pneumonia and mycoplasma pneumonia,klebsiella pneumonia (KP),haemophilus influenza and legionella pneumophila by using indirect immunofluorescence test for the antibody detection (IgM).And a standard medical history was recorded,including epidemiological data,clinical manifestation,and the length of hospital stay,white blood count and chest X-ray elsewhere.All specimens were collected in Department of Pediatrics,Zhongnan Hospital of Wuhan University,from Jan.2009 to Dec.2009.Results Flu A was detected in 234 of the 599 (39.1%,234/599 cases) flu-like children.The mean age was 48.8 months (range from 3 to 167 months).The detection rate in less than 1-year-old group (11.1%,18/162 cases) was obviously lower than that of the older groups (P < O.001).Especially for the group < 6 months,there was only 1 infant (1.43%,1/70 cases) with Flu A positive.Compared with Flu A negative group,the main clinical features of children with Flu A positive group were fever(78.2%,183/234 cases,P =0.013) and cough(76.5%,179/234 cases,P =0.04),however asthma (14.1%,33/234 cases,P =0.003)and diarrhea (6.0%,14/234 cases,P = 0.001) cases were less.The detection rates of Flu A reached a peak of 57.0% in November and December,and a secondary peak of 45.0% in May and June.Flu A infection children less than 1 year old were simple in etiology.The children were older,the etiology was more complex.The primary coinfection pathogen was KP (53.8%,126/234 cases) and Flu B(48.3%,113/234 cases).Compared Flu A coinfection with or without KP,there was no difference between these 2 groups in the clinical features.Conclusions Intfluenza A is the main pathogen of hospitalized children with flu-like symptoms in 2009.Children over 1 year old age were the major infection groups of Flu A.The detection rates of Flu A reached peaks in May to June and November to December.Flu A positive children were prone to KP coinfection.
8.On the Construction of Practice Base of Professional Moral Education for Medico
Leban NONG ; Qiwei YUAN ; Jie WEI ; Litai HUANG
Chinese Medical Ethics 1996;0(01):-
Practice base of professional moral education of university is the important constituent of practice base of moral education of university. Practice base of professional moral education of university is a training place of undergraduate professional ideal、professional concept、professional attitude、professional discipline、professional style、professional value which be built by the nation、all levels of local government or university according as the moral education goal of university and the undergraduate body and mind law of development, specially the law of moral forms. The construction of practice base of professional moral education of university is a important action to enhance the actual effect of the professional moral education for medico. The construction of practice base of professional moral education of university ought to follow the policy of"being educated、enhancing ability、contributing" and the principle of "benefiting each other、adjust measures to local conditions and economy、understanding each other and integrating through free will". Finally the article discuss the pattern of the construction of practice base of professional moral education of university for medico and put forward three kind of patterns of the construction of practice base of professional moral education of universityaccording to the actual fact of the college.
9.The regulation of the TACE pro-domain to tumor necrosis factor-? converting enzyme activity
Xiaoou LI ; Yuan YAN ; Wei HUANG ; Yuzhen YANG ; Hongwei WANG
Chinese Journal of Pathophysiology 1986;0(04):-
AIM:To study functions of pro-domain in tumor necrosis factor-? converting enzyme (TACE) maturation and to develop an approach to interfere with inflammation processes. METHODS: The plasmid pIRES2-EGFP was used to generate the expression plasmids constructed with the signal peptide and pro-domain, full length or without the pro-domain(designated as pIRES2-EGFP/T648, pIRES2-EGFP/T2472, pIRES2-EGFP/T57-T1824,respectively). The plasmids were used to transfect the U937 cells.After the transfected cells were stimulated by LPS, the effect of expression plasmids on TNF-? secretion was detected by ELISA and flow cytometry(FCM).RESULTS: The plasmid pIRES2-EGFP/T648 inhibited 61.09% TNF-? secretion and mediated the accumulation of TNF-? on the surface of U937 cells.The sTNF-? secretion and the level of the mTNF-? in the plasmid pIRES2-EGFP/T57-T1824 transfected cells had no significant deviation compared with the pIRES2-EGFP transfected cells. The sTNF-? secretion of the cells transfected by the plasmid pIRES2-EGFP/T2472 increased and the level of the mTNF-? decreased.CONCLUSION: The pro-domain has dual effects in TACE maturation and might be applied to molecular design of anti-inflammation drug.
10.Refining technical preparation of gross specimen.
Yuan HUANG ; Wei-bo MAO ; Li-fei ZHOU
Chinese Journal of Pathology 2006;35(6):373-374