Arrhythmogenic Right Ventricular Dysplasia ; diagnosis ; Death, Sudden ; Humans ; Male ; Middle Aged

Adult ; Bronchoalveolar Lavage ; Bronchoalveolar Lavage Fluid ; cytology ; Humans ; Macrophages ; pathology ; Male ; Middle Aged ; Pneumoconiosis ; pathology ; therapy

Objective To investigate the extraction of DNA from urine and urine stains and typing.Methods DNA was extracted from urine and urine stains with Chelex-100 extraction method and QIAamp Mini Kit.STR loci were typed after amplification by PCR procedures with Profiler Plus Kit.Results STR loci could be well typed in DNA extracted from the urine,which was fresh or preserved properly for less than 12 hours.Satisfactory DNA typing could be made in 50% of the urine preserved for two days.No typing results were obtained in the samples kept for seven days or much longer time,and typing was rarely available using urine stains.Conclusion DNA extracted from the fresh urine can be typed,which may be used in forensic identification.

Objective: To study human peripheral blood dendritic cells(DC) inducing Th0 differentiation to Th1/Th2 at different DC/Th0 ration。 Methods: Mononuclear cells in healthy human peripheral blood were induced to DC through tradition method,4 days of culture of mixed DC/Th0 different ration( 1∶4 and 1∶300),harvesting Th,after 48 h of CD3 mAb and CD28 mAb expantion,in supernant IL-4 and IFN-? measurement were determined using ELASA。 Results:Expresse CD83(73.4%), CD86 (90.3%), HLA-DR(68.5%),CD40(73.7%).DC/Th0 ration(1∶4) produced IFN-? (34?4.3) ?g/L, a little amount of IL- 4(25?4.3) ng/L. DC/Th0 ration(1∶300) produced IFN-?(3.5?1.2) ?g/L, a large amount of IL- 4(350?120) ng/L. Conclusion:Highly mixed DC/Th0 ration induced Th0 differentiation to Th1, low mixed DC/Th0 ration induced Th0 differentiation to Th2, DC regulating immunology response type were plastic which provided the basis for clinic GVHD therapy.

Objective: To observe the rheological properties of octoxynol-9 vaginal thermosensitive in situ gel [(O-9)-VTG] for predicting the gelation behavior in vivo, and evaluate the retention ability in vagina.Methods: The rheological parameters of (O-9)-VTG and (O-9)-VTG diluted by stimulant vaginal fluid (SVF) were measured by a Haake Rheomix to characterize the rheological properties.The vaginal samples after the administration of self-made (O-9)-VTG and O-9 gel were withdrawn, and then the concentration of octoxynol-9 in the samples was determined to evaluate the retention ability.Results: (O-9)-VTG was Newtonian fluid with low viscoelasticity under room temperature and converted to gel at 32.6℃.The formula could still transform into gel at body temperature after diluted by SVF, and resided in the vagina of mice above 8 h.Conclusion: (O-9)-VTG has suitable gelation temperature and rheological properties.Compared with the self-made octoxynol-9 gel, (O-9)-VTG has satisfactory retention in vagina, which meets the requirements for vaginal topical use.

Objective:To prepare compound mupirocin film forming gel and establish the quality control methods. Methods:The compound film forming gel was prepared using tannic acid and salicylic acid as the esterifying agents interacting with hydroxypropyl cel-lulose followed by cross-linking with tartaric acid and sorbitol to form a new film forming material, and then antimicrobial mupirocin and anesthetic dyclonine were added to prepare compound mupirocin film forming gel. The contents of mupirocin and dyclonine were deter-mined by HPLC. The column was Hypersil ODS2 (250 mm × 4. 6 mm, 5 μm) and the mobile phase consisted of methanol-ammonium acetate buffer (1. 15 g ammonium acetate, 300 ml water, 1 ml glacial acetic acid) (75 :25). The flow rate was 1. 0 ml·min-1 and the detection wavelength was 230 nm. The column temperature was 30℃ and the injection volume was 20μl. Results:The film form-ing gel was yellow, brown and gelatinous at the normal temperature, and formed transparent film after coating on the skin. The linear range of mupirocin and dyclonine was 20-400μg﹒ml-1(r=0. 9999) and 10-200μg﹒ml-1(r=0. 9996), respectively. The aver-age recovery was 99.9%(RSD=0.54%, n=9) and 99.6%(RSD =1.45%, n =9), respectively. Conclusion: The preparation process is reasonable, simple and controllable in the preparation of ideal film forming gel.

Objective To investigate the effect of biofilm cleaning agents and multienzyme cleaning agents in the cleaning of tubular instruments. Methods About 200 pieces of intramedullary nail used in tibial fracture and intramedullary nailing were selected and divided into multienzyme cleaning group and biomembrane cleaning group according to the order of recovery. According to the random number table method, each group had 100 pieces. The biofilm cleaning group was cleaned with biological membrane cleaning agent, and the multienzyme cleaning group was cleaned with multienzyme cleaning agent. The cleaning effect of luminal instruments was observed by means of microscopy, dipstick test and ATP bioluminescence assay. Result The cleaning quality and biofilm removal effect of the biofilm cleaning group were better than those of the multienzyme cleaning group (P<0.05). Conclusion Cleaning the luminal instruments with biomembrane cleaning agent can improve the cleaning quality and prevent the formation of bacterial biofilm.

[Objective]To compare the immediate therapeutic effects of the treatment of gluteal muscle constracture(GMC)with arthroscopic release of contractured tissue and conventional surgery.To investigate the indications of arthroscopic surgery.[Method]Ninty-six limbs of 48 patients with traditional surgery and 84 limbs of 42 patients with arthroscopic surgery were analyzed in terms of operation time,length of the incision,postoperative pain,complications,the time of exercise,postoperative hospital stay and one-year recurrence rate.[Result]Arthroscopic surgery group had smaller incisions,earlier exercise,less postoperative pain and shorter hospital stay than conventional surgery group,but the two groups showed no significant difference in operative time,complications,effects of operation and one-year recurrence rate.[Conclusion]Arthroscopic release of contracture tissue is a safe,effective,economical and cosmetic way to treat the gluteal muscle contracture.This method is worthy of wide promotion.

Objective To investigate the role of PI3K/AKT and ERK1/2 signaling pathways in the process of cardiomyopathy induced by doxorubicin(DOX).Methods Twenty SD rats were divided into two groups:DOX group(10 rats that were injected through caudal vein with 2.5 mg/kg DOX,once a week,for 6 weeks)and control group(10 rats).At 5th week after the end of DOX injection,the structural changes and functional damage were examined,and the apoptosis rate of cardiomyocytes and the expression levels of p-AKT and p-ERK1/2 were determined.Results Eleven weeks after the DOX injection,the myocardial pathological changes were mainly myofibrillar loss and cytoplasmic vacuolization.DOX could induce compensated heart function(the value of FS% was 34,P0.05)and Bax to 338%(P