Objective The measurement of normal tibia was aimed to obtain the value of the posterior slope angle of the medial tibial plateau in Chinese adult and its related parameters in order to improve the design of knee prosthesis more suitable for Chinese people, and also provide an essential features of tibia for resecting tibial plateau in total knee arthroplasty. Methods 450 knees of 428 persons which were healthy Chinese adult long living in North China, were 252 male knees and 198 female knees with an average age of 43.9 years ranging from 18 to 79 years. The criteria of the subjects were no tibial deformity, no genu varum and valgum, abnormal changes of articular surface, and had sustained ever any tibial trauma. Depending on the age of subjects, they were divided into three groups, group A was between 18 and 39 years, group B between 40 to 59 years, and group C more than 60 years. Moreover, 6 tibias harvested from healthy Chinese corpses were measured as well. Computer radiography was used to take standard lateral aspect of X-ray film. The imagines were stored into computer by scanning the films, and so the measurement was taken in computer using Danzig method. The data were analyzed statistically. Results By two factors analysis, the sex and age had no significant influence of the posterior slope angle of the tibia; furthermore, the angle was not different between the left and right tibia. In male adult, the average degrees of left posterior slope angle of the medial tibial plateau were 13.2??4.4?, the right 10.5??5.1?, and the average degrees of posterior slope angle of both sides were 11.7??4.9?. In female adult, the average degrees of left posterior slope angle of the medial tibial plateau were 11.2??3.5?, the right 10.6??4.4?, and the average degrees of posterior slope angle of both sides were 10.8??4.0?. The average degree of group A were 11.7??4.4?, group B 10.4??4.7?, group C 12.3??4.4?. In summary, there was no significant difference of posterior slope angle of the medial tibial plateau between sex, side and age. Conclusion The average degrees of posterior slope angle of the medial tibial plateau in North China adult were 11.3??4.5? ranging from 0??21? to 9?-13?, and were larger than that of the Westerns.

Environmental Monitoring ; methods ; Noise, Occupational ; statistics & numerical data

In order to observe the pial and intracortical vessels in the upper and lower lips of calcarine sulcus, the normal human visual cortex of 15 brains (30 sides) were studied with scanning electron microscope (SEM), staining for alkaline phosphatase activity, continuous arterio-venous perfusion and clearing of thick sections according to Malligan's procedure. The results are: (1) Superficial pial arteries on visual cortex originate from calcarine artery, parieto-occipital artery, and inferior posterior temporal artery. Their branches destribute on the surface of the upper and lower lips of the calcarine sulcus resembling a comb. Two types of arterial anastomoses were found. The pial veins of the upper lip of the calcarine sulcus empty into the medial parieto-occipital vein, while those of the lower lip empty mostly into the medial occipital vein. (2) Intracortical arteries might be divided into five groups, i.e, short cortical artery, middle cortical artery, long cortical artery, subcortical artery and medullary artery. In general, they send out forward and recurrent branches. Veins might also be classified into five groups accordingly. There are four layers of vascular network within the striate cortex. The 3rd layer was clearly divided into sublayer 3a, 3b, 3c. The capillary density of the sublayer 3b is with lower density, which is just situated in Gennari's band. Angioarchitecture in the unstriate cortex is loosely arranged. The vascular density of each layer has been measured. The relationship between the blood supply of visual cortex and its clinical significance has been discussed as well.

Purpose:To evaluate the effect of oxaliplation (L-OHP)+flurouracil/calcium folinate(5-FU/CF) in combination with concurrent radiotherapy for advanced rectal cancer and to find a more effective way of giving them.Meth- ods:Chemotherapy:L-OHP 130 mg/m~2,iv infusion for 2 hours D_1 combined with calcium folinate 200 mg/m~2 for 2 h ours followed by 5-fluorouracil 300 mg/m~2 for 4h D_1～D_5,repeated every 3 weeks.Radiotherapy:The whole pelvic irradiation DT 50～60Gy/25～30/F(5～6 weeks).Results:30 advanced rectal cancer patients entered the study.One patient reached complete response(CR),15 partial response,13 stable disease,and 1 with progression after treatment,The overall re- sponse rate(CR+PR) was 53.3%,the 1-year survival rate was 70% (21/30).The main adverse acute effects were gas- trointestinitis,anemia,neuro-sensory toxicity and irradiation rectitis,bone marrow suppression was mild.Conclusions: This approach of therapy could improve the therapeutic effect on advanced rectal cancer.

Objective To investigate the expression of vascular endothelial growth factor-C(VEGF-C) and matrix metalloproteinase-7(MMP-7) in gastric carcinoma and their correlation with tumor invasion and(metastasis).Methods Streptavidin peroxidase immunohistochemistry technique(SP)was used to detect the(expression) of VEGF-C and MMP-7 in 60 gastric carcinoma specimens,60 specimens of gastric mucosa(adjacent) to carcinoma,and 30 regional lymph node specimens.Results The positive expression rate of VEGF-C,MMP-7 in gastric carcinoma was markedly higher than that in normal gastric mucosa and gastric mucosa adjacent to carcinoma.The positive expression rates of VEGF-C and MMP-7 in metastatic regional lymph nodes were significantly higher than that in non-metastatic regional lymph nodes(P

Objective To evaluate the effect of the serum of rats with hepato-pulmonary syndrome (HPS) on the expression of caveolin-1 and VE-cadherin in pulmonary microvascular endothelial cells (PMVECs).Methods Among the 40 healthy Sprague-Dawley rats,aged 3-4 months,weighing 220-250 g,20 rats were taken randomly for establishment the model of HPS which was produced by chronic ligation of the common bile duct,and the left 20 rats served as sham operation group.Primary PMVECs were harvested from healthy adult Sprague-Dawley rats and inoculated in ECM culture medium or on 96-well culture plate.The PMVECs of 4th-9th generation were randomly divided into 2 groups (n =36 each):control group (group C) and HPS group.In group C,the serum obtained from normal rats in sham operation group was added to PMVECs,while the serum obtained from rats with HPS was added in HPS group.The final concentration of serum was 10％.After being incubated for 12,24 and 36 h (T1-3),the expression of caveolin-1 and VE-cadherin in PMVECs was detected by Western blot,and the PMVEC adhesion rate and proliferation were determined by CKK-8 method.Results Compared with group C,the expression of caveolin-1 and VE-cadherin was significantly down-regulated,the cell adhesion rate was decreased,and the proliferation of PMVECs was enhanced in HPS group.Conclusion The serum of rats with HPS induces weakened PMVEC contact inhibition through down-regulating caveolin-1 and VE-cadherin expression.

BACKGROUND:The researches about the effect of retinoic acid on the proliferation of adipose-derived stem cells are rare, and the researches on the testosterone are mainly on the inhibition of cellaging. OBJECTIVE: To study the effects of retinoic acid and testosterone or combination on the cellcycle of adipose derived stem cells. METHODS:Adipose derived stem cells were isolated from adult female Sprague Dawley rats with 2 months age and cultured in vitro til passage 3 adipose derived stem cells, and then the 3rd passage adipose-derived stem cells were performed with adipogenic induction, osteogenic induction and surface marker identification. The cells were divided into six groups:(1) Control group;(2) 10-5 mol/L retinoic acid group;(3) Retinoic acid group;(4) 10-5 mol/L retinoic acid+testosterone group;(5) 10-6 mol/L retinoic acid+testosterone group;(6) Testosterone group. The adipose-derived stem cells in the control group were cultured with Dulbecco’s modified Eagle’s medium+10%fetal bovine serum culture medium, and the adipose-derived stem cells in the other five groups were induced with corresponding dose of retinoic acid and testosterone on the basis of control group. After cultured for 36 hours, the flow cytometry was used to detect the changes of cellcycle. RESULTS AND CONCLUSION:Compared with the control group, cellproportions in phase G 1 of 10-5 mol/L retinoic acid group and 10-6 mol/L retinoic acid group were increased significantly, and the cellproportions in phase S were decreased. Compared with control group, the cellproportion in phase G 1 of testosterone group was significantly reduced, and the cellproportion in phase S was increased. Compared with 10-5 mol/L retinoic acid group and 10-6 mol/L retinoic acid group, cellproportions in phase G 1 of 10-5 mol/L retinoic acid+testosterone group and 10-6 mol/L retinoic acid+testosterone group were reduced significantly and the cellproportions in phase S were increased. Retinoic acid can inhibit the cellcycle of adipose-derived stem cells in phase G 1 , and delay the process of the cellcycle from phase G1 to phase S;while testosterone can promote the cellcycle of adipose-derived stem cells from phase G1 to phase S;the combination induction of retinoic acid and testosterone can accelerate the process of the cellcycle of adipose-derived stem cells from phase G 1 to phase S.

Objective To compare the effects on blood lipid level by immunosuppressive drugs in renal transplantation recipients. Methods Two hundred and eighty-three renal allograft recipients with tacrolimus(FK506), cyclosporine A(CsA) and rapamycin (SRL) immunosuppressive regimen were reviewed in this study. The variation of whose total cholesterol(TC) and triglyceride(TG) concentration in serum were compared before and after three immunosuppressive regimen. Results There was no significant difference in TC and TG before and after oral FK506 for 93 patients[(4.9± 1. 1) and (1. 4±0. 8)mmol/L vs (4. 9±1.1) and (1.4±1.0)mmol/L, respectively, P>0. 05]. The concentration of TC and TG from 106 patients with CsA[(4. 8±1. 0) and (1. 6±0. 8)mmol/L vs (6. 6±1. 7) and (3. 2±1. 0)mmol/L, respectively] and 29 patients with SRL was higher than those before taking drugs, P<0. 05. The concentration was increased after 12 to 24 weeks generally. The concentration of TC and TG of CsA from FK506 to tacrolimus for 51 patients[(6. 7±1. 1) and (2. 8± 1. 0)mmol/L vs (4. 7±1. 7) and (1. 5±1. l)mmol/L, respectively] were decreased after 12 weeks (P<0. 01). Conclusions Primary factor of dyslipidemia was that CsA and SRL were used for patients post-renal transplantation, which should be regarded. The FK506-based immunosuppressive regimen should be recomended in renal transplantation patients who have a hyperlipidmia.

Adult ; Aged ; Cerebrovascular Circulation ; drug effects ; Drugs, Chinese Herbal ; therapeutic use ; Female ; Hemorheology ; Humans ; Male ; Microcirculation ; drug effects ; Middle Aged ; Migraine Disorders ; drug therapy ; physiopathology ; Nails ; blood supply ; Phytotherapy

Objective To observe the expression of P21 and CDK6 in cervical squamous cell carcinoma,and to investigate the relationship between their expression and cervical squamous cell carcinoma.Methods The expression of CDK6 and P21 in 100 cases of cervical squamous cell carcinoma,20 cases of cervical CIN lesions and 20 cases of normal cervical tissues were detected by immunohistochemical ABC method,and the relationship between them and tumor differentiation,invasion depth,lymph node metastasis and clinical stage were analyzed.Results The expression rates of P21 and CDK6 in cervical squamous cell carcinoma tissues were significantly higher than those in CIN lesions and normal cervical tissues;The low expression of P21 was associated with the depth of tumor invasion,lymph node metastasis and clinical stage (P＜0.05),and was not associated with the degree of tumor differentiation (P＞0.05);The high expression of CDK6 was associated with tumor differentiation,invasion depth,lymph node metastasis and clinical stage (P＜0.05).Conclusion The abnormal expression of P21 and CDK6 may play an important role in the occurrence and development of cervical squamous cell carcinoma,and the two may have a certain significance in the prognosis of cervical squamous cell carcinoma.