Objective To set telephone alarm device in attraction system of automatic control center for timely detection of malfunctions.Methods The vacuum negative pressure meter and the delayed control line were used.The telephone key of H.F.was connected and the dial key was stored.Results Malfunctions could be detected promptly through telephone alarm.Conclusion The attraction system of the center can be examined and repaired in time,thus ensuring the medical security.

砄bjective:To study the biological characteristics and mineralization activity of strom cells (MSC) isolated from rat bone marrow.Methods: MSCs were isolated from rat bone marrow using gradient centrifuge (460 g,20 min) with 70% percoll and cultured with DMEM. The cells were then incubated with mineralization culture medium (MCM) in the presence of Dex (10～7 mol/L), ? GP (10 mmol/L) and AcA (50 g/L).Cell growth was studied with cell counting,alkaline phosphatase (ALP) expression with a kit and mineralization with a saturated solution of alizarin red S (pH 4.3) stainning.Results: The isolated cells from bone marrow of rat were epithelial like. The doubling time (DT) of the cells in MCM or DMEM were 50.3 h and 66.1 h respectively. Mineralization was observed after the cells had been cultured in MCM for 28 days. bFGF and rhBMP 2 increased ALP expression of MSC in a dosage dependent manner. Conclusion: In vitro cultured MSCs may be induced to differentiate towards osteoblasts.

Objective:To study the biological characteristics and mineralization activity of strom cells (MSC) isolated from rat bone marrow.Methods: MSCs were isolated from rat bone marrow using gradient centrifuge (460 g,20 min) with 70% percoll and cultured with DMEM. The cells were then incubated with mineralization culture medium (MCM) in the presence of Dex (10～7 mol/L), β-GP (10 mmol/L) and AcA (50 g/L).Cell growth was studied with cell counting,alkaline phosphatase (ALP) expression with a kit and mineralization with a saturated solution of alizarin red S (pH 4.3) stainning.Results: The isolated cells from bone marrow of rat were epithelial-like. The doubling time (DT) of the cells in MCM or DMEM were 50.3 h and 66.1 h respectively. Mineralization was observed after the cells had been cultured in MCM for 28 days. bFGF and rhBMP-2 increased ALP expression of MSC in a dosage dependent manner. Conclusion:In vitro cultured MSCs may be induced to differentiate towards osteoblasts.

OBJECTIVETo evaluate the efficacy and the relevant effect factors of acupuncture for type 2 diabetes mellitus (T2DM) in females.

METHODSOf 83 female patients of T2DM, 49 cases were deficiency of kidney yin syndrome and 34 cases were yin and yang deficiency syndrome. Acupuncture was adopted and the acupoints were selected according to the syndrome differentiation. In kidney yin deficiency syndrome, Taixi (KI 3), Shenmen (HT 7), Taichong (LR 3) and Sanyinjiao (SP 6), etc. were selected. In yin and yang deficiency syndrome, Shenshu (BL 23), Pishu (BL 20), Yishu (Extra) and Jingmen (GB 25), etc. were selected. In 3 courses of treatment, the changes in fasting plasma glucose (FPG), fasting insulin (FINS), insulin sensitivity index (ISI), insulin resistace index (Homa-IR), index of islet beta-cell function (Homa-beta), total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C) and high density lipoprotein cholesterol (HDL-C) were compared before and after treatment between the two groups. The relationship of the efficacy was analyzed in terms of the syndrome differentiation, age, duration of sickness, obesity and hereditary factors.

RESULTSAfter the treatment, the levels of FPG, FINS, Homa-IR, TC, TG and LDL-C were reduced obviously (all P < 0.01) and the levels of ISI, Homa-beta and HDL-C were increased apparently (all P < 0.01). The total clinical effective rate was 80.7% (67/83), in which, that of kidney yin deficiency syndrome was 83.7% (41/49) and that of yin and yang deficiency syndrome was 76.5% (26/34). The efficacy was not different significantly between the two syndromes. But, the younger the age was, the better the efficacy was.

CONCLUSIONAcupuncture positively regulates the glucose and lipid metabolism in the patients of T2DM. This therapy improves insulin resistance, enhances the body sensitivity to insulin and improves insulin beta-cell function. The efficacy is related to the patient's age.

Acupuncture Points ; Acupuncture Therapy ; Adult ; Aged ; Blood Glucose ; metabolism ; Cholesterol ; blood ; Diabetes Mellitus, Type 2 ; metabolism ; therapy ; Female ; Humans ; Middle Aged ; Treatment Outcome ; Triglycerides ; blood ; Young Adult

Objective To evaluate the efficiency of different wash methods for removing organophosphorus pesticide residues from cucumber. Methods The cucumber samples were soaked with tap water, washed rice water, salt solution and detergent solution respectively for 2 to 15 minutes then organophosphorus pesticide residues in the soak water samples were determined with ultrasonic extraction and gas chromatography. Results Soaked in tap water for 2 to 5 minutes was the most effective and convenient way to remove methamidophos and dimethoate with the highest removing rate (about 70%). Conclusion Soaked with tap water for 2 to 5 minutes is more efficient for removing organophosphorus pesticide residues from vegetable.

To study the alkaloid constituents in the seed of Sophora alopecuroides L. and isolation of lehmannine by transhydrogenation of sophocarbine to matrine present in the mixture. Methods　Various processes of exchange, ion exchange and column chromatograph with very similar physical and chemical properties was successfully separated by transhydrogenation. Results　5 alkaloids were obtained and identified by elementary analysis, IR, MS, 1HNMR and 13CNMR as oxymatrine, oxysophoridine, matrine, sophoridine and lehmannine (12, 13-dehydromatrine). Conclusion　Lehmannine was isolated from the seed of S. alopecuroides for the first time.

Object To study the alkaloid constituents in the seed of Sophora alopecuroides L and isolation of lehmannine by transhydrogenation of sophocarbine to matrine present in the mixture Methods Various processes of exchange, ion exchange and column chromatograph with very similar physical and chemical properties was successfully separated by transhydrogenation Results 5 alkaloids were obtained and identified by elementary analysis, IR, MS, 1HNMR and 13 CNMR as oxymatrine, oxysophoridine, matrine, sophoridine and lehmannine (12, 13 dehydromatrine) Conclusion Lehmannine was isolated from the seed of S alopecuroides for the first time

Objective:To investigate the effect of 5F on proliferation and apoptosis of human pancreatic cancer.Methods:The pcDNA3.1-PUMAAS and pcDNA3.1 were transfected into AsPC-1 cells by Lipofectamine 2000 methods,stable transfected clony was chosen through G418.AsPC-1 cells were divided into three groups:transfected pcDNA3.1-PUMAAS,transfected pcDNA3.1 and control group without transfection AsPC-1 cells.Three groups was treated with serial concentrations(8.875,37.5,142?mol/L,respectively)of 5F 10?L.MTT assay was used to observe the inhibitory actions of 5F on AsPC-1 cells.The apoptotic rate of the cells was detected by flow cytometry.And the apoptosis was assessed by Hoechst 33258 dye staining and TUNEL staining.The expression of PUMA protein was detected by Western blotting and RT-PCR.Results:The inhibitory action on cell growth was seen in AsPC-1 control cells and pcDNA3.1-cells dealing with 5F.It could also promote the occurrence of apoptosis.5F inhibited the proliferation of AsPC-1 cells in a concentration-dependent manner.The apoptosis induced by 5F was accompanied with the up-regulation of PUMA.In cells treated with 5F,the apoptosis rate decreased greatly and proliferation rate increased compared with the AsPC-1 control cells and pcDNA3.1-cells dealing with 5F.The expression of PUMA was decreased greatly comparing with pcDNA3.1-cells treated with 5F,Conclusion:5F can depress the proliferation of AsPC-1 cell in vitro,mainly through the induction of apoptosis,and it was a potential agent for pancreatic cancer chemotherapy,the mechanism was probably related to its effect on the regulation of PUMA expression.

Objective: To explore the effect and mechanism of DMDAI-L in inducing the HL-60 cells apoptosis. Methods:Caspase-3 activity in HL-60 cells was measured with the enzymatic visible substrate DEVD-pNA. The fluorescence changes of mito-chondrial membrane potential (△Ψm) in HL-60 cells were investigated with the fluorescent probe JC-1. Results:The caspase-3 activ-ity was significantly increased in HL-60 cells after the DMDAI-L treatment at the concentration of 1. 25, 2. 5, 5, 10 and 20μg·ml-1 for 24h(P<0. 05). DMDAI-L could significantly reduce the mitochondrial membrane potential in HL-60 cells. Conclusion: The mechanism of DMDAI-L in inducing HL-60 cells apoptosis may involve the activation or regulation of caspase-3 activity as well as the reduction of mitochondrial membrane potential in HL-60 cells within certain concentration and time range.

Rheumatoid arthritis (RA) is a kind of chronic, progressive, multiple, invasive autoimmune disease with two chief cclinical manifestations arthrosynovitis and ex-arthrosis, easy to occur in middle-aged women, also occur in children and the elderly, is characterized by progressive and break out repeatedly. RA pathogenesis is complex, there is no special treatment, used in treatment of R drug varied, new drugs and new therapies also emerge in endlessly, main including non-steroidal anti-inflammatory drugs (NSAIDs), slow action anti-rheumatism medicine (SAARDs), glucocorticoids (GCs), biological agent, traditional Chinese medicine and traditional Chinese medicine preparations, domestic market for rheumatoid main drug treatment are NSAIDs, SAARDs, GCs, traditional Chinese medicine and traditional Chinese medicine preparations. Traditional Chinese medicine and traditional Chinese medi- cine preparations for the treatment of RA have its unique advantages, show the characteristics of overall adjustment, multi-level and multiple targets, and also can alleviate and against side effects of western medicine. In recent years, more and more get people's atten- tion. This paper reviewed the research progress and treatment features of commonly used therapeutic agents for the treatment of RA in recent years, which provides reference and basis for future medicine anti-RA.
Animals ; Arthritis, Rheumatoid ; drug therapy ; Biological Factors ; adverse effects ; therapeutic use ; Drug Discovery ; methods ; Glucocorticoids ; adverse effects ; therapeutic use ; Humans ; Medicine, Chinese Traditional ; adverse effects