A 28 days growth and development experiment and a 90 days intoxication experiment with —hydrochloro lysine at various dosage levels on young and adult rats were reported.The results were as follows. There were definitely an accelerating effect on growth and development in young rats when adding 0.5% L-lysine to a cereal diet, but no obviously similar effect in adult rats; moreover, a massive dose of L—lysine could induce hepatic and renal damage. The hepatic lesions were focal necrosis and increase of fat—storing cells with collagen fiber formation. In the kidney there were cloudy swelling, microvilli losing, intracellular edema, mitochondrial swelling, endopl asmic reticulum dilatation and residual body formation in the proximal convoluted tubule epithelium.

Objective To investigate the effect of rosiglitazone on the expression of platelet CD40 ligand (CD40L) in insulin-resistant rats, and to further determine the relationship between CD40L and insulin resistance. Methods 60 healthy male SD rats [(200±20)g] were randomly divided into 4 groups: control group (C), high fat group (HF), low dose rosiglitazone group (LR) and high dose rosiglitazone group (HR). Rats in group C were fed normal chow diet, and the others were given high fat chow diet. After 12 weeks, high dose of rosiglitazone (10mg/kg) was given to rats in group HR and low dose of rosiglitazone (5 mg/kg) was given to rats in group LR for 4 weeks. Rats in group HF and group C were given 0.9% sodium chloride solution. The level of sCD40L was measured by ELISA and the expression of platelet membrane CD40L was detected by immunoprecipitation and Western blot. The insulin resistance (IR) index was calculated by homeostasis model assessment (HOMA). Results HOMA-IR, sCD40L level and platelet membrane CD40L expression were higer in group HF than in group C (9.8±3.2 vs. 5.9±1.7, 367.3 ±35.3 vs. 232.3±120.6, 2.1±0.4 vs. 1.4±0.2, respectively, all P<0.05). Compared with the group HF, HOMA-IR, sCD40L level and platelet membrane CD40L expression were obviously decreased in group HR(5.4±1.1, 276.9±54.0, 1.4±0.3, respectively, all P<0.05), and there were no significant differences in HOMA-IR, sCD40L level and platelet membrane CD40L expression between group HF and group LR (P>0.05). Conclusions In insulin-resistant rats, the level of sCD40L and the expression of platelet membrane CD40L were higher. After treatment with high dose of rosiglitazone, sCD40L level and platelet membrane CD40L expression were decreased with the improvement of insulin resistance.

ObjectiveTo observe the curative effect of intensive regulating lipid by atorvastatin in the treatment of unstable angina and its influence on serum high sensitive C-reactive protein(hs-CRP).Methods60 patients with unstable angina were randomly divided into two groups:the routine treatment group( basic treatment plus atorvastatin 10mg/d),the atorvastatin intensive treatment group( basic treatment plus atorvastatin 40mg/d.)The courses of treatment were both 8 weeks.The differences in the amount of serum hs-CRP,blood fat,the effect of electrocardiogram and the use of glyceryl trinitrate before and after the treatment were observed and compared.Results The differences in the angina curative effect and the amount of serum hs-CRP between the two groups were statistically sig nificant( all P ＜0.05 ).ConclusionShort-term intensive regulating lipid by statins was effective in treating unstable angina,and could quickly relieve the phlogosis in the vessels,prevent the unstable plaque from breaking and the thrombopoiesis,and thus prevent the occurrence of cardiovascular disease.

IL-18 participates in the morbility and exacerbation of acute pancreatitis,and induces the organ-injury of extra-pancreas.The aim of this review is to explore the source of and the regulation of activation and secretion of IL-18,as well as the pathogenesis of acute pancreatitis-associated liver injury induced by IL-18.

Breast tumor is the most common malignant tumor in female,its incidence is increasing in recent years.With magnetic resonance equipment,image acquisition and processing technology is improved,perfusion imaging studies gradually become the focus of study.The author reviewed MRI perfusion imaging and its application in breast tumors.

Objective To explore the treatment of kala-azar with antimonial resistance in children. Method The clinical data of antimony resistant Kala Azar in a child was analyzed retrospectively, and the related literature were reviewed. Results A 2-year- and 5-month-old boy, suffered from fever, pancytopenia and hepatosplenomegaly. He was diagnosed with kala-azar by bone marrow examination, and improved after the treatment of adequate antimonial. Later, he relapsed twice and the treatment of adequate was effective.. This was the third relapse, and was considered as antimonial resistance. Liposomal amphotericin B (1 mg/kg on day 1, 2 mg/kg on day 2, 3mg/kg on day 3~7 and day 10, with accumulated dose of 21 mg/kg) was used and effective. The child improved and no relapse in one-year follow-up. Conclusion Liposomal amphotericin B can be used in the treatment of kala-azar with antimonial resistance in children.

Fiber bronchoscopy has been used in pediatric for decades,and has became the important diagnostic tools in pediatric respiratory diseases.with the improvement of fiber bronchoscope equipment and the advancement of operation technology,fiber bronchoscope has been expanded unceasingly in pediatric, especially in the critically ill children.As a common critical disease in pediatric,severe pneumonia is urgent, serious and high rate of mortality.As the effect of conventional antibiotic treatment is not ideal in current, while fiber bronchoscopy with the dual function of examination and treatment makes it use more and more widely in children with severe pneumonia.Fiber bronchoscopy can improve the effect of treatment in children with severe pneumonia,short the course of the disease,improve the prognosis of children,reduce the rate of mortality.

Objective To evaluate the change situation of cognitive function in the patients with chronic renal failure (CRF) complicating obstructive sleep apnea syndrome(OSAS) .Methods Sixty‐two inpatients with non‐dialysis CRF in our hospital from September 2013 to January 2015 were selected and divided into the group O (complicating OSAS ,31 cases) and group N(without complicating OSAS ,31 cases) ,and contemporaneous 33 individuals undergoing the physical examination were taken as the control group(group C) .The cognitive function was evaluated by the Montreal cognitive assessment (MoCA) .The apnea hypopnea index (AHI) and lowest pulse oxygen saturation(LSpO2 ) at night were measured with a portable sleep monitor .The comparative analysis was performed .Results In the group O ,there was 14 cases of patients(45 .16% ) whose MoCA total score was less than 26 points , while there was only 3 cases(9 .68% ) in the group N ,the difference was statistically significant (P<0 .05) .The MoCA total score , scores of visuospatial and executive function ,attention ,delayed recall and orientation in the group O and N were lower than those in the group C ,while the MoCA total score ,attention and delayed recall in he group O were lower than those in the group N ,the score of language was lower than that in the group N and C ,the differences were statistically significant (P< 0 .05) .The MoCA total score was negatively correlated with serum creatinine and AHI (r= -0 .374 ,r= -0 .408 ,P<0 .05) ,and positively correlated with the GFR level and night LSpO2 (r=0 .382 ,r=0 .519 ,P<0 .05) .Conclusion The patients with CRF complicating OSAS increase the risk of cognitive impairment .

The incidence of local recurrence after wide local excision and radiation of soft tissue sarcoma (STS) ranges from 5％ to 20％.The optimal management of locally recurrent STS must be individualized.Approaches for retreatment include wide local re-excision followed by a variety of radiation which include external-beam radiation,brachytherapy and intraoperative electron radiotherapy.Following retreatment,the likelihood of ultimate local control ranges from 37％ to 100％.However,each radiation technique could produce severe side effects,and so for selected patients,repeat irradiation may be unnecessary.