Objective To study the effect and superiority of interferon used for treating bronchiolitis by aerosol inhalation.Methods 86 patients with bronchiolitis were divided into the two groups,the therapy group with 42 cases,which were received 20μg interferon by aerosol inhalation two daily for 7 days,the control group with 44 cases,which were given 50mag ribavirin by aerosol inhalation two daily for 7 days.The time of asthma and rales disappear in lungs and temperature decresing and hospitalization were observed.Results The time needed for the remission and disappearance of the major symptoms and physical signs like asthma and rales in the therapy group were significantly shorter than those in the control group,there were significant differences between the two groups(t =2.413,2.547,all P ＜0.01).The total effective rates of the therapy group was 88.0％ (37/42),while of the control group was 47.7％ (21/44),there were significant differences(x2 =15.59,P ＜ 0.01),no side effect was found.Conclusion Interferon can be effectively treated bronchiolitis by aerosol inhalation.It is worthy of application.

Objective To investigate the effects of calcitonin gene-related peptide (CGRP) combined with norepinephrine (NE) on L-type calcium current (LCa-l) in rat ventricular myocytes. Methods Ventricular myocytes were isolated from SD rats (weighing 260-280 g) by retrograde perfusion of the heart via the aorta with an enzyme-containing solution as previously described. Whole-cell patch-clamp recording was made using Axopatch 200B amplifier. The cells were perfused for 1 min with Tyrode solution containing CGRP 1 × 10-7 mol/L (group CGRP) , NE 1 × 10-6 mol/L (group NE), or CGRP 1 × 10-7 mol/L + NE 1 × 10-6 mol/L (group CN) and again washed with Tyrode solution. ICa-L was recorded 1 min before and 1 min after the cells were perfused and 1 min after the cells were washed. I-V curve of ICa-L was made after the cells were perfused with solution containing CGRP or NE alone. Results CGRP significantly inhibited the peak of ICa-L, while NE significantly promoted the peak of ICa-L(P < 0.05) . The peak of ICa-L was significantly decreased 1 min after the cells were perfused in group CGRP,while increased 1 min after the cells were perfused in group NE compared with group CN ( P < 0.05). CGRP made the I-V curve of ICa-L move up-ward, while NE made the I-V curve of ICa-L move down-ward. Conclusion CGRP can weaken the promotion of ICa-L induced by NE in rat ventricular myocytes.

Objective To observe and evaluate the efficacy of the preventing antibiotic-associated diarrhea in infant with bacterial pneumonia using the live Clostridium butyricum and Bifidobacterium combined Powders. Methods 103 infants with bacterial pneumonia were randomly divided into the two groups,53 infants as the trial group,another 50 infants as the control group with normal antibacterial therapy,two or three hours afterword the live Clostridium butyricum and Bifidobacterium combined Powders was administered at dose of 0.5g ti-daily in the trial group,After diarrhea taken place,the live Clostridium butyricum and Bifidobacterium combined Powders was adminis-tered at dose of 0.5g ti-daily in the control group,to contrast the rate of secondary diarrhea and clinical efficacy in the two groups.Results The incidence of antibiotic-associated diarrhea was 13.2%( 7/53 ) in the trial group and 56.0%(28/50) in the control group (χ2 =21.00,P<0.01).There were significant differences between the two groups.Duration of diarrhea and period of treatment in the trial group was obviously shorter than that of the control group,the rate of dehydration in the trial group was lighter than that in the control group(χ2 =6.64,P<0.01). Conclusion The live Clostridium butyricum and Bifidobacterium combined powders with antibacterial therapy at reg-ular intervals can prevent antibiotic-associated diarrhea.It is worthy of preventive application.

Objective To investigate the treatment of moed high anal fistula operation.Methods 110 cases of high anal fistula patients were randomly divided into the incision line selective suture group(treatment group 55 cases) and incision group(55 cases in the control group),on two groups of treatment time,the cure rate,the anal function were analyzed.Results The cure rate of the treatment group and control group were 98 ％,86％,respectively,the cure rates of two groups have no significant difference (x2 =1.557,P ＞ 0.05),the healing time and postoperative anal function evaluation in treatment group was superior than those in control group (t =3.440,4.671,all P ＜ 0.01).Conclusion Incisionunder selective suture in the treatment of high anal fistula operation is a better way.

Objective To evaluate the changes of the level of cerebrospinal fluid T-tau protein andβ-amyloid 42 in mild cognitive impairment (MCI) patients. Methods Computer retrieval was preformed for cerebrospinal fluid (CSF) to-tal tau protein (T-tau) and β-amyloid 42 (Aβ 42) levels among MCI and healthy people in Pubmed, Cochrane Library, Ovid, CNKI, VIP Data and Wangfang Data. Meta-analyses were conducted on the T-tau and Aβ42 levels using Review Manager 5.2 software. Results Seventeen studies met inclusion criteria. Meta-analysis results showed that, CSF T-tau protein levels were significantly higher (MD:113.10;95%CI:87.93-138.26) and Aβ42 levels were significantly lower in the patients with MCI, (MD=-146.50;95%CI=-178.70- -114.29) compared with the control group (P<0.01). Conclu-sions T-tau significantly increases while Aβ42 significantly decreases in the CSF in patients with MCI.

Objective To discuss the influence and significance of stromal cell-derived factor 1 (SDF-1) and its specific receptor CXC chemokine receptor 4 (CXCR4) in proliferation, migration and invasion ability of SW480 colorectal cancer cells. Methods The colorectal cancer cell line SW480 in logarithmic phase was divided into four groups:control group (with no any processing), SDF-1 group (added 100μg/L SDF-1), SDF-1+1 mg/L AMD3100 mixed group (added 1 mg/L AMD3100 for 2 hours, then added 100μg/L SDF-1) and AMD3100 group (added 1 mg/L AMD3100). Immunohisto?chemistry method was used to detect the protein expression of CXCR4 in SW480 cells. The expression of CXCR4 mRNA in SW480 cells was detected by RT-PCR before and after SDF-1 and AMD3100 treatment. MTT assay and transwell chamber were used to test the changes of proliferation, migration and invasion ability of SW480 cells before and after SDF-1 and AMD3100 treatment. Results The result of immunohistochemistry showed that CXCR4 protein was expressed in SW480 cells (positive rate=80%). CXCR4 mRNA was expressed in SW480 cells. The expression of CXCR4 mRNA was up-regulat?ed by SDF-1(100μg/L), which could be inhibited by AMD3100 (1 mg/L). The proliferation activity was higher in SDF-1 group (0.847±0.039) compared to that in control group (0.624±0.011) and SDF-1+AMD3100 mixed group (0.607 ±0.016). The proliferation activity was lower in AMD3100 group (0.456 ± 0.031) than that in control group and SDF-1+AMD3100 mixed group (F=108.03, P<0.05). The number of transmembrane cells was more in SDF-1 group (98.7±5.8, 33.7±6.2) than that in control group (21.0±2.2, 6.1±2.3), SDF-1+1 mg/L AMD3100 mixed group (18.5±8.4, 8.5±2.8) and AMD3100 group (12.1±3.2, 2.1±1.0) detected by transwell chamber experiment. However, there were no statistical differences between three groups. Conclusion The biological axis SDF-1/CXCR4 can promote the proliferation, migration and invasion in colorectal cancer cell line SW480.

Objective:Two experiments were performed to research the effects of visual field factor on pseudoneglect and to test the theories about neglect and pseudoneglect.Methods:Experiment 1 employed the landmark task and greyscales task in tachistoscopic and forced-choice conditions.Experiment 2 employed the greyscales task and tachistoscopic visual three-field technique.Results:Experiment 1 manifested that greyscales had no crossover effect;the length factor had effect on the greyscales task as the landmark task,but it only affected the bias magnitude,not the error direction.Experiment 2 indicated that the main effect of visual field was significant:when the greyscales were presented in the middle or right,pseudoneglect manifested,but when in the left,pseudoneglect vanished.Conclusion:The results provide evidence supporting the hypothesis that the perceptual distortion derives from the attentional process.

Objective To investigate the effects of adenovirus vectors for BTB and cap'n'collar protein homology 1 (Bach1) small interfering RNA (siRNA) on antioxidanffactors and fibrosis related cytokines in lung fibroblasts (MLF) in transforming growth factor (TGF)-β1 induced mouse.Methods Bach1 siRNA recombinant adenovirus vectors and blankadenovirus vectorwere constructed,then the MLF cells were incubated with TGF-β1 (5 ng/ml) for 24 h and infected with blankvector and successful constructed Bach1 siRNAs.The messenger RNA (mRNA) and protein expression of Bach1,heme oxygenase (HO)-1,glutathione peroxidase 1 (GPx1) and nicotinamide-adenine dinucleotide phosphate:quinone oxidoreductase-1 (NQO1) in cell supernatants were determined by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot.Changes of fibrosis-related cytokines including TGF-β1 and interleukin (IL)-6 levels in cell supernatants were determined by enzyme-linked immunosorbent assay (ELISA).One-way analysis of variance (ANOVA) was performed for multiple group comparisons and LSD test was used to compare the two groups.Results Bach1 mRNA (2.127±0.089) and protein expression increased significantly after TGF-31 stimulation compared with blank group (1.000±0.067,t=-21.77,P＜0.01),as well as the expression of fibrosis-related cytokines TGF-β1 (52±6) and IL-6 (34±6) in cell supernatants increased significantly after TGF-β1 stimulation compared with blank group (26±4,t=-11.11,P＜0.01 and 20±5,t=-5.32,P＜0.01),but the mRNA expression of HO-1 and GPx1 (0.403±0.040 and 0.567±0.112) also the protein expression decreased significantly compared with mRNA (1.000±0.181,t=25.57,P＜0.01 and 1.000±0.212,t=6.68,P＜0.05) and protein expression in blank group.Follow the Bach1 siRNA treatment,Bach1 mRNA (0.153±0.015) and protein levels were significantly downregulated compared with mRNA (2.129±0.089 and 1.973±0.035,F=1835.95,P＜0.01) and protein expression of TGF-β1 and blank vector group,as well as TGF-β1 (26±3) and IL-6 (11±3) expression in cell supernatant were significantly inhibited compared with TGF-31 (52±6 and 34±6) and blankvector group (49±5 and 33±6) (F=22.25,P＜0.01 and F=28.38,P＜0.01).But the mRNA levels of HO-1 (3.303±0.294) and GPx1 (1.840±0.231) in MLF were promoted significantly compared with TGF-β1 (0.403±0.040 and 0.567±0.112) and blank vector group (0.353±0.057 and 0.667±0.090) (F=53.90,P＜0.01 and F=526.25,P＜0.01).Conclusion Silencing Bach1 rescues TGF-β1 induced reduction of antioxidants and increasethefibrosis in MLF cells.The study offers an experimental basis to explore pathogenesis of oxidative stress and antioxidant therapy for connective tissue disease related inter-stitial lung disease.

The evaluation of outcomes of cancer therapy in clinic are largely based on volumetric and morphological evidences. These evidences, however, are very limited in assessing the early effects of therapy. Functional and molecular MR imaging allows co-registration of functional/ molecular-based information with high-resolution anatomical detail within the same imaging modality, which makes it possible for assessing the early effects of tumor therapy.

Objective: To evaluate the feasibility of diffusion weighted imaging (DWI) application in follow-up of patients receiving transcatheter arterial chemoembolization (TACE) for liver cancer and its clinical value. Methods: DWI and routine non-enhanced MRI were performed in 16 liver cancer patients after TACE with the same SE-EPI sequence (b=500 s/mm2 and 0) on 1.5 T MRI. The numbers of the new lesions detected by DWI, T1 WI and T2 WI were counted. Contrast noise ratios (CNR) between new lesions and liver tissues on DWI and T2 WI were calculated and compared. The efficacy of SWI in diagnosis of residual tumor and recurrent cancer was qualitatively evaluated by comparing with DSA results. Results: DWI revealed 46 new lesions, T2 WI revealed 43, and T1 WI revealed 38. There was significant difference in CNR between new lesions and liver tissues on DWI and T2 WI (P < 0.05). The signal intensities of liver cancer on DWI were complicated after TACE. Homogeneous accumulation of iodized oil was observed on DSA in 2 of 16 patients with liver cancer after TACE; there was no tumor staining on DSA at the corresponding site of homogeneous hypointensity on DWI. Partial defect of accumulation of iodized oil was noticed in 2 of 16 patients; there was tumor staining on DSA at the corresponding site of hyperintensity on DWI. No or faint accumulation of iodized oil was noticed in 12 patients; there was strong tumor staining at the corresponding site of hyperintensity on DWI; necrosis tumor had no or slight tumor staining on DSA at the corresponding site of hypointensity on DWI. Conclusion: Diffusion MRI is a sensitive method for detecting new lesions in patients with liver cancer after TACE, and can be used to monitor residual, recurrent cancer and iodized oil accumulation during follow-up of patients after TACE.