Objective To observe the safety and severity of heat-sensitive moxibustion in treating poisonous snakebites-induced bladder dysfunction of different degrees.Method Twenty-six patients diagnosed with bladder dysfunction due to poisonous snakebites were examined by type-B ultrasonic scanning for residual urine volume and uroflowmetry. The patients were treated with heat-sensitive moxibustion. The residual urine volume and uroflowmetry parameters were compared between different severity degrees of bladder dysfunction before and after treatment.Result The residual urine volumes were changed significantly in the mild group and moderate group after treatment (P<0.01); the residual urine volume showed insignificant change after treatment in the severe group (P>0.05). The maximum urine flow rate (Qmax) was significantly changed in the mild group after the treatment (P<0.05); the Qmax was insignificantly changed in the moderate and severe groups after treatment (P>0.05).Conclusion Heat-sensitive moxibustion is significantly effective in treating mild-moderate bladder dysfunction due to poisonous snakebites, and it’s safe and reliable.

Influenza,caused by influenza virus,is a respiratory infectious disease with a serious hazard to human health.Prevention of influenza through vaccine development is the most effective strategy.It is important to build a rapid response platform for research and production of influenza vaccine.As virus vectors,live vaccine provides a new prevention and treatment way for infectious disease.Modified vaccinia virus Ankara(MVA) is a replication-deficient viral vector that is safe and can encode one or more foreign antigens and induce humoral and cellular immune response.MVA holds great promise as a vaccine platform.In this review,we discuss the use of MVA for vaccine development against influenza virus.

The concept of far lateral lumbar disc herniation was firstly reported by Abdullah in 1974. The diagnosis of far lateral lumbar disc herniation is based on the discography findings as well as on clinical manifestations. Far lateral lumbar disc herniation is a special type of lumbar disc herniation. Clinically, far lateral lumbar disc herniation is less seen than other types of lumbar disc herniation, it accounts for 0.7%-11.7% of total lumbar intervertebral disc protrusion. Although the incidence of far lateral lumbar disc herniation is lower, its symptoms are usually heavier and its protrusion sites are specific. Its treatment methods are more complex, including mainly conservative treatment, minimally invasive interventional therapy and surgical management, etc. This paper aims to make a review about the recent progress in its corresponding treatment.

Glucocorticoid is an important treatment for many pediatric diseases and it often leads to osteoporosis, osteonecrosis of the femoral head, and growth retardation. Among them, glucocorticoid-induced growth retardation has been a particular focus in the treatment of children patients, which leads to a decrease in final height. The mechanism of glucocorticoid-induced growth retardation is complex, and there is no safe and effective treatment.This article summarized the mechanisms underlying glucocorticoid-inhibited longitudinal bone growth and reviewed the potential therapeutic targets discovered in recent years.

Objective To investigate the effect of Tianqi Jiangtang capsules combined with metformin on blood viscosity, cognitive disorder in elderly patients with type 2 diabetes mellitus complicated with cerebral microvascular lesions.Methods 76 patients with type 2 diabetes mellitus complicated with cerebral microvascular lesions were divided into the observation group and the control group according to odd and even numbers, 38 cases in each group.The observation group were given Tianqi Jiangtang capsules combined with metformin while the control group were received metformin alone.The clinical curative effect, adverse reactions were compared between the two groups.The total score of TCM symptoms, blood glucose, blood lipid, high-sensitivity C reactive protein(hs-CRP), hemorheology and Mini Mental State Examination(MMSE)scores were determined before and after treatment.Results The total effective rate in the observation group(89.47%)was significantly higher than that in the control group(71.05%)(P< 0.05);After treatment, the total score of TCM symptoms, FPG, 2 h PG, hs-CRP, TC, TG, LDL-C, whole blood viscosity at high shear, whole blood viscosity at low shear, plasma viscosity and hematocrit were significantly lower than those before treatment, while MMSE scores were significantly higher(P< 0.05), and the above indexes in the observation group after treatment were significantly better than those in the control group(P< 0.05).There were no significant adverse reactions in the 2 groups.Conclusion Tianqi Jiangtang Capsules combined with metformin is safe and effective in the treatment of elderly patients with type 2 diabetes mellitus complicated with cerebral microvascular lesions.It can significantly improve lipid metabolism and hemorheology, reduce blood viscosity, and alleviate cognitive disorder.

Objective To investigate the effect of tirofiban hydrochloride in the treatment of coronary heart disease and its influence on homocysteine level.Methods 92 patients with coronary heart disease were selected as research subjects.They were randomly divided into the observation group and the control group,46 cases in each group.The control group was treated with anti-thrombotic,nitrates and other conventional western medicine.The observation group was treated with tirofiban hydrochloride based on the conventional treatment.Results The total effective rate of the observation group was 95.65%,which was higher than 82.61% of the control group,the difference was significant (χ2=4.039,P<0.05).And the homocysteine level in the observation group was (5.38±1.02)μmol/L,which was lower than (7.27±1.19)μmol/L in the control group,the difference was statistically significant (t=8.179,P<0.01).After treatment,the cardiac function CO level[(4.96±0.98)L/min],EF level[(62.48±11.16)%]and SV level[(86.37±11.47)mL]in the observation group were higher than those in the control group,the differences were statistically significant (t=2.271,2.954,2.911,all P<0.05).After treatment,the hs-CRP level[(4.25±1.32)mg/L]and Fib level[(3.14±0.73)g/L] in the observation group were lower than those in the control group,the differences were statistically significant (t=7.981,3.174,all P<0.01).ConclusionTirofiban hydrochloride in the treatment of coronary heart disease can effectively contribute to the reduction of homocysteine levels and alleviate atherosclerosis lesion progression,not only can reduce severity of myocardial ischemia and fibrinogen and high-sensitivity C-reactive protein levels,but also can reduce the thrombosis and inflammation produced by the degree of harm to the patients.

Objective To revise the Research Capacity Self-Rating Scales of Nursing Staff and test its reliability and validity.Methods Based on the definition of scientific research capacity,the initial dimensions and items of Research Capacity Self-Rating Scales of Nursing Staff was formed by literature review,semi-structured interview and experts evaluation.The content validity was evaluated by experts,and the face validity by nursing staffs.600 nurses were investigated by convenient sampling.The reliability and validity and discrimination of the scale were tested.Results Research Capacity Self-Rating Scales of Nursing Staff were selected in 7 dimensions including the capacity of find problem,research design,scientific research practice,innovation,information retrieval,data processing and writing.Scale-level Content validity index was 0.94.The Cronbach alpha coefficient of Research Capacity Self-rating Scales of Nursing Staff was 0.964,the Cronbach alpha coefficients of the seven dimensions were 0.771-0.975,split-half reliability was 0.885.Seven factors were extracted by using principal factor analysis,cumulative contribution was 71.002％.The correlation coefficient between the Scientific Research Capacity Self-rating Scales of Nursing Staff and General Self-Efficacy Scale was 0.649 (P=0.000).The discrimination was better.Conclusions The Research Capacity Self-rating Scales of Nursing Staff revised shows good reliability and validity.It can objectively evaluate scientific research capacity of nursing staff and provide scientific reference for further improving the research capacity of the nursing staff.

Objective To investigate the synaptopathy of hidden hearing loss mice,and to observe the synapses of the cochlear inner hair cell after temporary threshold shift of noise exposure.Methods Mice were divided into normal control group and experiment group,the latter was exposed under noise of 98 dB SPL for 2 h to establish the model of temporary threshold shift.Mice cochleae of the two groups were dissected and prepared with whole mount and immunostaining.Cellular morphology was observed under confocal laser scanning microscope.Cochlear lengths were measured through cochlear frequency map to localize hair cells in different frequency regions.Then,3-D morphometry of synapses was constructed by Amira software to observe pre-synaptic ribbons,post-synaptic receptors and its pathological changes.Results In control group,each cochlear nerve fiber contacted a single inner hair cell by a single synapse,each inner hair cell had 5-30 synapses contacting cochlear nerve fibers.The larger ribbons patched smaller receptors located in the modiolar side,and the smaller ribbons patched larger receptors located in the pillar side.While in experiment group,noise overexposures caused moderate or completely reversible thresholds shift,i,e.,distortion product otoacoustic emission (DPOAE) and auditory brainstem response (ABR) thresholds increased 30-40 dB.Although returned to normal after 2 weeks,ABR wave Ⅰ amplitudes recovered to only 46.1 ％ of pre-exposure amplitudes.There was 41.3％ synapses loss of inner hair cell,but there was no loss of inner hair cells and spiral ganglion neurons.Conclusions Threshold test is not sensitive to degeneration and loss of synapse in mice inner hair cells,while super threshold test is sensitive to it.

Objective To investigate the changes and significance of I?B kinase-?(I?K-?) in the lung tissues of rabbits with hemorrhagic shock.Methods The expressions of I?K-? and NF-?B in the lung tissues and the concentration of tumor necrosis factor-?(TNF-?) in the plasma were measured by in situ hybridization(ISH),immunohitochemistry and enzyme linked immune adsorbent analysis(ELISA), respectively. And the pathological changes were examined with light microscope in lung tissues.Results In hemorrhagic shock group,the expressions of I?K-?(0 1685?0 0164)and NF-?B( 0 1469?0 0083)in lung tissues , the level of TNF-?(636 72?100 23) in the plasma were obviously higher than those of normal group [I?K-?(0 0427?0 0241),NF-?B(0 0358?0 0048),TNF-?(199 51?35 69)ng/L](all P

Objective To examine the effect of all-trans-retinoic acid (atRA) on proliferation and apoptosis rates of smooth muscle cells in healing vein bypass grafts.Methods Autogenous vein graft model was established in 40 rats by transplanting the internal branch of the jugular vein to the carotid by end-to-end anastomosis.Animals were divided at random into two groups:atRA group and control group.The animals received atRA(10mg?kg -1 ?d -1 ) or same dosage vehicle(corn oil) from 4 days preoperation to 10 days postoperation.Animals were sacrificed and the grafted veins were harvested at 7,14 days,respectively after the operation .The grafted veins were then processed for staining and measurements.Hyperplasia,smooth muscle cell proliferation were detected by pathological and immunohistochemical methods.All the data were analyzed by a computerized system.The presence of apoptotic smooth muscle cells was demonstrated by terminal deoxynucleotidyl transferase biotin nick end-labeling (TUNEL) method.Results There was a significant decrease in the average intimal thickness at 7,14 days in the atRA group.Immunohistochemical analysis of proliferating cell nuclear antigen (PCNA) indicated decreased positive cells in the atRA group, compared with the control group at 1 or 2 weeks after the operation. Apoptosis of smooth muscle cells was higher in the atRA group than in the control group at 1 week or 14d postoperation.Conclusions These preliminary results demonstrated that atRA (10mg/kg/d) inhibits smooth muscle cell proliferation and induces smooth muscle cell apoptosis in rats.