Objective:To investigate the association of a(TA)n dinucleotide repeat polymorphism with unknown aetiology hypomenorrhea.Methods:100 normal menstruation person(control group) and 100 hypomenorrhea patients(case group)were recruited.they all came from southen-western China.The(TA)n dinucleotide repeat in ERa gene upstream hypervariable region were purifed,cloned and sequence analysed.we observe the ERa gene(TA)n dinucleotide repeat polymorphism distribution in the two groups.Results:All samples exhibted 7 different alleles.The(TA)n dinucleotide repeats were 11~17,The most prevalent allele was TA14 repeat in control group.The distribution of the different alleles of the(TA)n dinucleotide repeat sequence in the group and control group has significant difference(?2=13.412,P =0.037).Fisher's exact test was applied to compare the frequency of each a1lele between two groups.The frenquency of the TA13 allele was found to be significantly greater in hypomenorrhea group(P =0.006),and the frenquency of the TA15 allele was less in the group(P =0.033).Conclusion:A(TA)n dinucleotide repeat sequence which is in ERa gene upstream hypervariable region was associated with unknown aetiology hypomenorrhea.The TA13 allele was found to be associated with unknown aetiology hypomenorrhea and may be a risk factor;whereas TA15 allele may be a protective factor for patients.

0.05).The expressions were remarkably of endometrial ER、VEGF and KDR in the study group as extremely lower than those in the control group,and the statistical difference between the two groups were all significant(P0.05).Conclusion:ER、VEGF and KDR may be relevant to the occurrence of hypomenorrhea.

Objective To investigate various sex hormones and the their relation in patients with polycystic ovary syndrome (PCOS).Methods 40 patients with PCOS were matched with 40 age matched healthy women,study group was divided into the obese group and non-obese groups based on body mass index,insulin resistance and non-insulin-resistance based on insulin sensitivity index. The level of follicle stimulating hormone (FSH), luteinizing hormone (LH), prolactin (PRL), estradiol (E2), testosterone (T), androstene dione (A4), sulfal-dehydroepiandrosterone (DHEAS), sex hormone-binding globulin (SHBG), fasting glucose(FG), fasting insulin (FIN) was measured in both group. Homeostasis model assessment insulin resistance (HOMA-IR), insulin sensitivity index (ISI) and free androgen index (FAI) were caulculated. Results ①In the PCOS group,LH, LH/FSH, T, A4, FAI, FIN and Homa-IR were significantly higher compared to the control,while FSH, SHBG, ISI were significantly lower (P<0.05),PRL, FG, E2, DHEAS level did not show difference (P>0.05). ②There were significant differences in T, FAI, A4, but not in DHEAS between the PCOS group and the control one. ③There were significant differences between the hirsute group and the non-hirsute group in T, FAI, A4 (P<0.05). ④In the obese PCOS group compared to the non-obese PCOS group, T, A4, DHEAS were not significantly different, but FAI and Homa-IR were significantly higher, SHBG, ISI, LH/FSH were significantly lower. ⑤The quantity of insulin resistance in the increased T group was significantly higher compared to the common T group. ⑥In the insulin resistance group compared to the non insulin resistance group, there were not significant differences of A4、DHEAS,T and FAI was significantly higher.⑦In PCOS group,there were significant positive correlation between FAI useful parameter compared to T, A4, DHEAS for the diagnosis of PCOS. Obese PCOS women have more severe and BMI,FAI and Homa-IR;BMI and LH/FSH were significant inverse correlation. Conclusions FAI is more endocrine secretion and metabolic disturbance than non PCOS women. Hyperandrogenism and insulin resistance are consanguineous correlation.

Four fresh corpses of infant are used in this research. Three-dimensional microvasculatures of the sinoatrial node and atrioventricular node were studied by means of the observation of vascular casts with SEM. The results showed that the vascular bed of the sinoatrial node was consisted of the microvascular networks. It was oval in shape. The central artery penetrated this node, through its longitudinal axis and divided progressively into arterioles and precapillary arterioles and finally, they branched into capillary networks at the superficial part of the node. The postcapillary venules were characterized by draining blood in accordance with area. The microvascular construction of atrioventricular node was an oblate microvascular network in shape. There was a layer of delicate capillary networks at its superficial part. In the depth of the capillary networks, the venous plexus which was thick and sinusoidal in shape was observed through the meshes. The artery of atrioventricular node entered the node from one side of it. In the node, the artery ramified by degrees to periphery into precapillary arterioles, which penetrated venous plexus and connected with capillary networks in the superficial part of the node. There were evidently narrow rings, whick were impression of the muscle sphincters at the origin of thep ostcapillary venules.

Objective To investigate the association between serum N-terminal pro brain natriuretic peptide(NT-ProBNP) and creatine kinase myocardial(CK-MB),cardiac troponin I(cTnI) in children who were diagnosed as viral myocarditis(VMC),and to explore the clinical significance.Methods Fifty children with VMC were selected as VMC group who were further divided into cTnI(+)group and cTnI(-)group.Fifteen healthy children were selected as healthy control group.The levels of myocardial enzyme of the 2 groups were tested by automatic biochemical analyzer and level of NT-proBNP was detected by specific NT-proBNP enzymelink immunosorbent assay(ELISA) kit.SPSS 13.0 software was used to analyze the data.Results 1.There were 23 cases with positive cTnI and 27 cases with negative cTnI.The levels of NT-proBNP in VMC group including cTnI positive and cTnI negative were significantly higher than those in healthy control group (P0.05).2.In the group with positive cTnI,the level of NT-proBNP was correlated with cTnI.The coefficient of determination was 0.17.3.In the group with negative cTnI,the level of NT-proBNP was correlated with CK-MB.The coefficient of determination was 0.34.NT-proBNP presented a increasing trend during interval of 25-100 U?L-1 and 175-255 U?L-1 of CK-MB.Conclusions The levels in the serum NT-proBNP in VMC children were higher than those in healthy children.Serum detection NT-proBNP may be applied as a new approach in diagnosis of VMC in children.

Animals ; Diet, Atherogenic ; Disease Models, Animal ; Heart ; physiopathology ; Myocardium ; pathology ; Obesity ; etiology ; physiopathology ; Rats ; Renin-Angiotensin System ; physiology

Three alkaloids were isolated from Huperzia serrata (Thunb. ) Trev. Their structure were identified as 8-deoxyserratinine (Ⅰ), lycodiue (Ⅱ) and phlegmariurine B (Ⅲ ) by means of spectral analysis (UV, IR, NMR, MS ). compoundⅡ and Ⅲwere isolated for the first time from this species

Four alkaloids were isolated from Huperzia serrata (Thunb. ) Trev., The structures of these alkaloids were identified as des-N-methyl-?-obscurine (Ⅰ),lycopodine (Ⅱ),lycodoline (Ⅲ),and 6-?-hydroxy-lycopodine (Ⅳ) by means of spectral analysis (UV, IR, NMR, MS ). Compounds Ⅰ and Ⅳ were isolated for the first time in the species.

OBJECTIVE: To study the effects of total saponins of Panax japonicus (TSPJ) on human leukemic HL-60 cells. METHODS: Human leukemic HL-60 cells were cultured in vitro. The cancer cell vigor was detected by using cell counting kit-8. Nitroblue tetrazolium (NBT) was used for measuring cell reduction. The cell cycle and the expression of differentiation antigen CD11b were detected by flow cytometry. RESULTS: Compared with the negative control group, TSPJ in different concentrations could decrease the vigor of HL-60 cells and the number of cells in S phase and up-regulate the CD11b expression, while the numbers of NBT positive cells and cells in G(0)/G(1) phase in the different concentrations of TSPJ-treated groups were increased. CONCLUSION: TSPJ can inhibit the HL-60 cell growth in vitro. Its mechanism may be related to inhibiting proliferation and inducing cell differentiation and cycle arrest.

The occurrence rate of DTC in children and adolescents is not high.However,DTC in these patients has some distinct characteristics different from those in adult,such as larger tumor volume at diagnosis,early invasion of neck lymph nodes and early distant metastases,high NIS expression,high recurrence but higher overall survival rate.131 I ablation is still one of the important treatment methods after surgery.Currently,there are 3 major dosage regimens adopted for 131I treatment: prescribed dose without causing bone marrow suppression/toxicity,dose aiming at tumor ablation,and empirically fixed dose.