Objective:To investigate the effects of Urocortin-postconditioning on mitochondria-dependentapoptotic pathway.Methods: 24 Wistar rats were randomly divided into 3 groups(n=8):Control group(CON),I/R group(I/R),Urocortin group(UCN).The left ventricular samples in the Control group were collected as pre-ischemia controls through thoracotomy.After isolated heart Langendoff and Neely models were established,the rat hearts in the I/R group and the UCN group were continuously perfused with Krebs-Henseleit (K-H)buffersolution for 30 min,then the rat hearts were arrested by cardioplegia,St.Thomas-2 solution(STH-2)for 30min.After that,the rat hearts in the I/R group were reperfused with K-H buffer solution for 90 min.The rat hearts in the UCN group were reperfused with K-H buffer solution containing 10-8 mol/L Urocortin for 90 min.The releasing of Cyto C protein into cytosol was detected by Western blot.The expression of Caspase-3 protein in cardiomyocyte was detected by immunohistochemical assay.The apoptosis index (AI)of cardiomyocyte was detected by TUNEL.Results:Compared with Control group,the expression ofCaspase-3 and Cyto Cprotein in the I/R group and the UCN group were significantly increased(P

Objective Hemophagocytic lymphohistiocytosis (HLH) is a life-threatening condition characterized by excessive inflammation, with a high incidence in children and a death rate of 40%.This study was to analyze the gene expression profile in child-hood HLH and explore the important pathways of childhood HLH using bioinformatic methods . Methods The childhood HLH gene ex-pression profile data GSE26050 were obtained from the Gene Expression Omnibus (GEO) database of the National Center for Biotechnolo-gy Information.Differentially expressed genes were identified with the GEO 2R online analysis tools released recently .The key pathways of the differentially expressed genes were investigated using the Kyoto Encyclopedia of Genes and Genomes ( KEGG) pathway enrichment a-nalysis. Results A total of 184 differentially expressed genes were identified , 126 upregulated and the other 58 downregulated .They were enriched in 3 pathways, including cytokine-cytokine receptor interaction , hematopoietic cell lineage and NOD-like receptor signaling pathways. Conclusion Bioinformatic tools allow the identification of the key genes and pathways associated with the development and progression of childhood HLH and point out the potential directions for researches on the mechanisms of childhood HLH .

Background Glaucoma is common blinding eye diseases characterized by chronic loss of retinal ganglion cells(RGCs).Currently glaucoma pathogenesis is not completely understood,heat shock protein 27 ( HSP27 )may be associated with the pathogenesis of glaucomatous optic neuropathy. Objective Through the establishment of a rat model of high intraocular pressure,detection of the expression of HSP27 antibody in serum and RGCs apoptosis to investigate the role of HSP27 in RGCs apoptosis. Methods Fifty-one clean Wistar rats were divided into high intraocular pressure group (34 rats)and sham operation group( 17 rats)using a random number table.An animal model of high intraocular pressure was established in the right eye by the application of bipolar underwater electrocoagulation on vein of sclera surface in the experimental group,and rats with conjunctiva incision only without electric coagulation were served as sham operation (control).The intraocular pressure of rats of the both groups including experimental and control rats was measured 1,2,4,6,8 weeks after operation and then the rats were sacrificed.1 ml serum was collected from these rats to determine the concentration of HSP27 antibody.The retinas of the rats were isolated and homogenated for the extraction and analysis of the retinal protein by Western blot.Apoptosis of RGCs were assayed by TUNEL.The use of the experimental animals followed the Regulations for the Administration of Affair Concerning Experimental Animals by Kunming Medical Collegc. Results Intraocular pressure was elevated significantly after modeling and remained a high value during the expcrimental duration,showing a significant difference among the different groups ( F =318.502,P＜0.01 ).However,no significant change in intraocular pressure before and after operation in the sham-operation group ( F =2.076,P ＞ 0.05 ).The serum concentration of HSP27 antibody was increased significantly overtime from 1,2,4,6 to 8 weeks after the surgery applied,with a significant difference among various time points( F =154.221,P＜0.01 ),but no obvious difference was seen in the shamoperation group( F =0.422,P＞0.05 ).Importantly,Apoptosis rate of RGCs in the experimental eyes was significant higher as the time prolong after intraocular elevated(x2=856.12,P＜0.05 ).The positive rate of RGCs apoptosis in the rats with high intraocular pressure group was elevated significantly compared to the rats in the sham operation group(P＜0.05). Conclusions High intraocular pressure induces the expressions of HSP27 in retina and the accumulation of HSP27 antibody in rats serum;the elevated HSP27 is associated with the increased cell death in RGCs.

The effects of polytetrafluoroethylene (e PTFE) membrane on periodontal ligament cell (PDLC) growth and periodontal tissues and new cement regeneration were studied, and the prospects of clinical application of e PTFE membrane were discussed. The results showed that PDLC grew well on the e PTFE membrane and no toxicity or inhibition was found. Close adhesion of the cells to the membrane was observed by scanning electron microscope. Histological studies suggested that the peculiar porous structure of e PTFE membrane protects and promotes healing of alveolar bone defect when the membrane edge was sealed perfectly on the crown side,exhibiting inhibition of growth of epithelium towards tooth root. The membrane was beneficial to regeneration of new alveolar bone and cement and establishment of new adhesion,and was regarded as a good substitute of old materials.

Objective To establish a method for the determination of eugenol in Xiao’er Fuxie Waifu Gel.Methods RP-HPLC method was developed with Lichrospher-C18 analysis column,using methyl alcohol-water(65:35) as mobile phase.The detection wavelength was 280 nm and flow rate was 1.0 mL/min.Results The linear range of eugenol was from 0.108 to 1.724 ?g(r= 0.999 9).The average recovery was 99.1 %and RSD was 1.4 %.Conclusion The method is convenient and accurate.It can be used for the quality control of Xiao’er Fuxie Waifu Gel.

BACKGROUND: Kyphosis in ankylosing spondylitis is sagittal or coronal imbalance, but there is a lack of study on its orthopedic biomechanics, and biomechanics is of great significance for the reconstruction of spinal stability after orthopedic surgery.OBJECTIVE: To establish a three-dimensional (3D) finite element model of kyphosis in ankylosing spondylitis treated by osteotomy on software, and to analyze its biomechanical properties, thus providing theoretical basis for clinical practice.METHODS: A 3D finite element model of kyphosis in ankylosing spondylitis was established based on CT data, and the predetermined angle of the osteotomy at L2 was measured. Afterwards, vertebral column decancellation and vertebral column resection were stimulated, and then the biomechanical parameters were analyzed. RESULTS AND CONCLUSION: (1) The 3D finite element models of kyphosis in ankylosing spondylitis treated by vertebral column decancellation or vertebral column resection at L3 were established successfully. (2) The stress on the screw and contact at each segment in the vertebral column decancellation group was significantly higher than that in the vertebral column resection group except for S1. (3) To conclude, both two methods can reconstruct the sagittal balance,but vertebral column decancellation exhibits significantly higher stress on the screw. Indeed, the incidence of internal fixation failure and complications in vertebral column decancellation is higher than that in vertebral column resection at the same segment and angle.

Objective To explore achillis tendon-sliding procedure and subtalar joint capsula release by the same cut and individualization treatment used in the correction of congenital clubfoot.Methods Forty eight cases (76 feet) of congenital clubfoot who were first visit were treated by achillis tendon-sliding procedure and subtalar joint capsula release by the same cut,meantime,footplate fascia release and transfer of the anterior tibial tenden were made by individualization.Results All cases were followed-up,the average time follow-up was 1 year and 6 months,excellent were 71% and good were 15.7%.Five cases were recurrence and its rate was 6.94%.Conclusions Achillis tendon-sliding procedure and subtalar joint capsula release by the same cut can solve rotation of displacement between calcaneus and talus and individualization treatment can also solve high arch deformity and adduction of foot.This technique is a simple,safe and effective treatment for congenital clubfoot.

In this paper,we made an analysis of the organization and content settings of medical laboratory students' summer clinical practices,and summarizes the students' achievements and experience of clinical practices in many years.Standardized organization and reasonable content settings are the guarantees for the students to have a comprehensive understanding of the basic major contents.Through the summer clinical practices,students can have a good understanding of their major in short period of time.

Objective To induce adult adult adipose-derived stem cells(ADSCs) in vitro to differentiate into neuronal-like cells,and to analyze the features of their cell morphology and ultrastructure. Methods Adipose stromal cells were obtained and amplified in vitro. Then make use of chemical induction to induce them. Observed ADSC and differentiation of cells in morphology and ultrastructure under inverted microscope and transmission electron microscopy. Immunocytochemistry to detection of Nestin, Neuron Specific Endolase( NSE) ,and glial fibrillary acidic protein(GFAP) expression in cells. Used Real-time PCR to detection of Nestin,GFAP gene mRNA expression before and after induction in ADSC. To observe the morphology and ultrastructure of the cells prior to and after induction under microscope and electron microscope. Results The morphology of ADSC was similar to fibroblasts ,and could be amplified stability within 10 passages in vitro. Some of the cells induced display a typical astrocyte-like cells in ultrastructure. Followed neuronal induction,astrocyte-like cells began to stain brightly for CFAP, Nestin. GFAP stained in both the cytoplasm and nuclei of astrocyte-like cells, but Nestin only stained in the cytoplasm. The peak positive expression rate within 14d following neuronal induction. The rate of positive expression cells was( 14.4 ± 3. 6) % for Nestin, (87. 3 ± 5. 3 ) % for GFAP. Then two kinds of protein expression remained the similar rate. The average relative concentration of GFAP and Nestin gene mRNA have significant statistical difference between ADSC and differented cells analyzed by Real-time PCR (P<0.05).The peak concentration of GFAP was within 20 d after induction,and GFAP was within 14 d after induction. Conclusion In the cytoplasm of adult adipose-derived cells possess Nestin genetic material,which is the marker of neural stem cell. The differential astrocyte-like cells have the typical morphology, ultrastructure and GFAP phenotype of mature astrocytes.

Objective To reseach the time point of the highest percentage of neural precursor cells derived from adipose stromal cells (ADSCs) in vitro, and to observe the ultrastructure features of neural precursor cells. Methods Used the β-mercaptoethanol to induce ADSCs to differentiate into neural precursor cells and neuron-like cells. The morphology of the uninductedcells and inducted cells were observed with inverted phase contrast microscope. The expression of nestin which was the marker of neural precursor cell in each group was detected using immunofluorescence staining method. The ultrastructural feature of cells which was induced for 3 hours were observed. Results The highest ratio of positive expression of nestin was 3 hours following induction,with the ratio ( 86.25 ± 4.82) %. There were many protuberance on the cell membrane under transmission electron microscopy.There were plenty of organelles in the neural precursor cells. The neural precursor cells had a large size nucleus,large nucleoplasmic index, much extended chromatin,and less condensed chromatin. The nucleus had double-layer nuclear envelope, more nuclear pore on the nuclear envelope. Conclusion The time point of the highest percentage of neural precursor cells derived from ADSCs is 3 hours,and the ultrastructral feature of induced neural precursor cells confirm that cells at this time point are in a state of split active period.