Background Free-flap Epi-LASIK surgery show a fast epithelial healing and mild trauma responses.Its mechanism is notable. Objective The aim of this study was to compare the corneal epithelial healing response following photorefractive keratectomy(PRK)and flap-free Epi-LASIK surgery. Methods Twelve experimental rabbits were randomly assigned to high cutting group(-10.00 D)and low cutting group(-3.00 D),and 2 normal matched rabbits were used as controls.PRK was performed in lateral eyes and flap-free epipolis laser in situkeratomileusis(Epi-LASIK)surgery was curried out in the fellow eyes.The corneal wound healing response in early stage of post-operation was examined under the slim lamp,and the inflammatory cells infiltration in cornea,basic fibroblast growth factor(bFGF)and nuclear transcription factor(NF-KB)expressions in corneal stroma were detected by hematine-eosin staining and immunohistochemstry in the seventh day after operation. Results The inflammatory reaction was milder in the eyes with Epi-LASIK than ones of PRK under the slim lamp.No significant difierences were found in numbers of inflammatory cells in cornea.expressions of bFGF and NF-κB in corneal stroma between two types of surgeries in low cutting group(P＞0.05).In high cutting group,the numbers of inflammatory cells were(12.25±1.22)/400 field in PRK eyes,and those of free-flap Epi-LASIK eyes were(6.67±0.31)/400 field,showing obvious difference between them(t=-8.87,P＜0.01).Expressions of NF-κB(A value)in cornea were 6.11±1.36 and 41.82±8.7 1 in PRK eyes of low cutting group and high cutting group.and those in free-flap Epi-LASIK were 3.01±0.81 and 11.59±4.55.indicating a drastically increase in PRK eyes(P＜0.05).The expressions of bFGF(A value)in corneal stroma were 33.59±6.98 and 123.68±18.81 in PRK eyes and those in free-flap Epi-LASIK eyes were 69.20±8.85 and 123.68±1 8.81 in low cutting group and high cutting group,presenting a considerably decrease in PRK eyes (P＜0.05).The corneal epithelial healing was faster in free-flap Epi-LASIK eyes than that in PRK eyes.and deeper cutting slowed the healing procedure. Conclusion In low cutting group,healing time of corneal epithelium is close between PRK and flap-free Epi-LASIK.But in high cutting group,epithelial healing is faster and inflammatory reaction is lighter in flap-free Epi-LASIK compared with PRK.

Objective:To analyze the effect of MCT/LCT fat emulsions at different doses on the growth and development of prema-ture and low birth weight infants. Methods: Totally 76 cases of preterm or low birth weight infants were divided into the treatment group (40 cases) and the control group (36 cases), both groups were given MCT/LCT fat emulsions, amino acid and glucose injec-tions in 12-24 h after the birth. The initial dose of MCT/LCT fat emulsions in the treatment group was 2. 0 g·kg-1 ·d-1 and the dose was gradually increased to 3. 0 g·kg-1 ·d-1 with 0. 5 g·kg-1 ·d-1 progressive increase. The initial dose of MCT/LCT fat emulsions in the control group was 0. 5 g·kg-1 ·d-1 , and the dose was increased to 3. 0 g·kg-1 ·d-1 with 0. 5 g·kg-1 ·d-1 progressive in-crease. The treatment course was 7 days. The neonatal weight, serum total bilirubin, direct bilirubin, alanine aminotransferase, total cholesterol, triacylglycerol and blood glucose were monitored in the two groups. Results:The born body mass recovery time of the ob-servation group was (4. 38+0. 93) d, which was significantly shorter than that in the control group [(6. 81+1. 90) d]. After the re-covery, the body weight growth rate of the observation group was (30. 41+1. 81) g·kg-1 ·d-1 , which was significantly higher than that of the control group [(18. 21 +2. 08) g·kg-1 ·d-1, P <0. 05]. After the 7-day treatment, the blood biochemical indices showed no statistically significant difference between the two groups (P>0. 05). Conclusion:Early application of MCT/LCT fat emul-sions at high dose is beneficial to the improvement of nutritional status in premature infants.

Objective: To investigate moderating role of sense of humor to the relationship between stressful events and mental health. Methods: 836 middle-school students were investigated by MSHS, ASLEC and GHQ-20. Results: Sense of humor had a positive effect on students’ mental health, and strong humor could deduce the level of anxiety and depressed emotion. Sense of humor could buffer the relationship of stressful events and depression, but it could not adjust the relationship of stressful events with self-affirmation and anxiety. Conclusion: Sense of humor has a moderating effect on the stressful events and some aspects of mental health.

Chemical investigation of the whole plants of Valeriana hardwickii has led to the isolation of 11 flavones and 2 monoterpe- noids by using various chromatographic techniques including column chromatography on silica gel and Sephadex LH-20, preparative TLC, and preparative HPLC. Their structures were identified by spectroscopic data analysis as syzalterin (1), 6-methylapigenin (2), 5-hydroxy-7,4'-dimethoxyflavone (3), genkwanin (4), acacetin (5), apigenin (6), quercetin (7), tricin (8), (-)-farrerol (9), sosakuranetin (10), 5,3',4'-trihydroxy-7-methoxyflavanone (11), (-)-bornyl ferulate ( 12) , and (-)-bornyl caffeate ( 13). All compounds were isolated from this plant for the first time, while compounds 1, 9-13 were obtained from this genus for the first time.
Drugs, Chinese Herbal ; chemistry ; isolation & purification ; Molecular Structure ; Spectrometry, Mass, Electrospray Ionization ; Valerian ; chemistry

Adolescent ; Child ; Child, Preschool ; Cytokines ; blood ; Female ; Humans ; Infant ; Interleukin-6 ; blood ; Interleukin-8 ; blood ; Male ; Seizures, Febrile ; immunology ; Tumor Necrosis Factor-alpha ; blood

Arthroplasty, Replacement ; Elbow Joint ; injuries ; surgery ; Humans ; Joint Instability ; therapy ; Tennis Elbow ; therapy

AIM:To investigate the influence of high-mobility group box 1 (HMGB1) on the proliferation of neural stem cells in peri-infarction cortex of focal cerebral ischemia/reperfusion model rats .METHODS: Male SD rats (n=48) were randomly divided into sham group , ischemia/reperfusion (I/R) group, RNA interference group and nega-tive interference group .The rat middle cerebral artery was blocked to establish focal cerebral I /R model ( ischemia for 1 h and reperfusion for 7 d).Lentivirus vector of HMGB1 shRNA was used to suppress the HMGB1 protein expression in the rat brain.The effect of RNA interference was evaluated by the methods of double-immunofluorescence labeling of HMGB 1/GFAP and Western blotting .The proliferation of neural stem cells in the peri-infarction cortex was assessed by double labe-ling of BrdU/nestin.RESULTS: The protein expression of HMGB1 in I/R group was much higher than those in sham group (P<0.05).RNA interference effectively inhibited the HMGB1 expression (P<0.05).Double labeled BrdU/nestin positive cells in I/R group were more than that in sham group (P<0.05).The double labeled BrdU/nestin positive cells were significantly decreased in RNA interference group (P<0.05).CONCLUSION:Focal cerebral ischemia/reperfusion injury promotes the proliferation of neural stem cells in peri-infarction cortex by increasing HMGB 1 protein level .

Objective To investigate the relationship between the MR findings of lumbar elements and chronic low back pain(CLBP).Methods The patients underwent lumbar MRI examinations and sent for a questionnaires of low back pain (LBP).Among them,139 patients whose questionnaires illustrated with CLBP were enrolled.The enrolled patients included 68 patients with nerve roots compression and 71 patients without.Meanwhile,198 hospital staffs underwent lumbar MRI examinations and were sentfor a LBP questionnaire.Among them,62 patients without LBP and nerve roots compression were enrolled.Categorical regression was used to analyze the relationship between MR findings and CLBP.The MR findings iucluded nerve roots compression,average disk degeneration scores(ADD),high-intensity zones (HIZ),Schmorlnodes,Modic Ⅰ change,average facet joints degeneration scores(AZZ),facet joint effusion,high T2 signal in interspinous ligament and subcutaneousparaspinal muscles edema.The regression model was used to analyze the MR imaging and CLBP.Results The regression model was statistically significant (F=9.478,P＜0.01).All predictors yielded an adjusted value was 0.446.Among all predictors,nerve roots compression,ADD,AZZ,subcutaneous or paraspinal muscles edema were statistically associated with the VAS degree (P＜0.05).The sum of the importance of the four predictors above was 0.983.The quantification of predicted VAS degree increased as ADD level increased.The quantification of predicted VAS degree increased to the top at the 2 AZZ level and then decreased.Nerve roots compression and Subcutaneous or paraspinal muscles edema yielded higher quantification of predicted VAS degree level.Conclusion ADD,AZZ,subcutaneousparaspinal muscles edema were probably associated with CLBP degree after adjusting for nerve roots compression.

BACKGROUND:It is an important issue of alveolar ridge preservation after tooth extraction. Because of the limited treatment and geographical conditions, lack of awareness of oral health, many local people in frontier areas in Xinjiang have poor alveolar ridge that is not of timely repair after tooth extraction. Thus, it is important to find a suitable local and efficient way to save the residual ridge, which has a more practical clinical value. OBJECTIVE:To investigate the feasibility of nano-colagen artificial bone used for alveolar ridge preservation in the Kazakh in Xinjiang Tacheng Region, China. METHODS: Sixty-eight Kazakh patients with bilateral extraction from Tacheng region, Xingjiang Uygur Autonomous Region, China were selected in a self-controled trial. According to the principle of a minimum alocation imbalance index, the experimental side and control side of extraction patients were confirmed. In the experimental side, nano-colagen artificial bone was implanted; while, conventional treatment was done in the control side. Multi-slice spiral CT was used to scan the regions of extraction interest to measure the relative gray value of alveolar bone mineral density immediately and 3 months after implantation. RESULTS AND CONCLUSION:Immediately after implantation, tooth extraction sockets were visible on CT images both in the experimental and control sides; but after 3 months, the extraction sockets became unclear on the CT images, and CT values were close to those of the surrounding alveolar process, but bone tissues were ful of the bone graft area in the experimental side. The alveolar bone mineral density was higher in the experimental side than the control side at 3 months after implantation. These findings indicate that the nano-colagen artificial bone has good clinical achievement in alveolar ridge preservation in the Kazakh in Xinjiang Tacheng Region.

Retinal vessel is the only visible vessels in human body and systemic microvascular lesions could be evaluated by the degree of retinal vessels. ln this article, the progress of retinal vascular caliber measuring method and its influencing factors were briefly reviewed.