Objective To screen a verification method for influenza virus inactivation in MDCK cells, and apply it to the sample detection before and after influenza virus inactivation, so as to provide a reliable method for the quality detection of influenza vaccines. Methods Four types of influenza viruses adapted to MDCK cells, B/Yamagata(BY), B/Victoria(BV),H1N1 and H3N2, were diluted to 10, 1, 0. 1, 0. 01, 0. 001, and 0. 000 1 lgCCID_(50)/mL separately, which were then inoculated into MDCK cells and cultured by changing the medium(discarding after adsorption for 1. 5 h and adding virus culture medium, passaging every 3 d for a total of 3 generations), rehydration method(adding virus culture medium directly after adsorption for 1. 5 h, passaging every 3 d for a total of 3 generations), and method in European Pharmacopoeia(passaging every 4 d for a total of 3 generations). The cytopathic effect(CPE) was observed every day, the virus solutions of different passages were harvested, and the hemagglutinin(HA) titers were detected to determine the optimum inactivation verification method. Finally, the optimum inactivation verification method was used to detect samples before and after inactivation of influenza virus. Results Influenza virus could be detected at the titer of 0. 1-1 lgCCID_(50)/mL by both changing medium method and rehydration method, while influenza virus could be detected in the titer range of 1-10 lgCCID_(50)/mL by test protocol in European Pharmacopoeia, and the titer of HA detected by rehydration method was higher than those by the other two methods. Therefore, rehydration method was determined as the optimum inactivation verification method. The samples detected by rehydration method were all positive before influenza virus inactivation and negative after virus inactivation.Conclusion Rehydration method is the optimum method to verify the influenza virus inactivation in MDCK cells, which can be used to detect the quality of influenza vaccines.

Aim To investigate the effects of propafenone on myocardium inotropism and explore it’s possible mechanism in isolated papillary muscle of guinea pig. Methods Developed tension (DT), maximum rate of contraction (+dT/dt_ max) and maxi-mum rate of relaxation (-dT/dt_ max) were measured during propafenone perfusion before and after administration of L-type calcium channel blocker, nicardipine and selective Na+/Ca 2+ exchanger inhibitor, KB-R7943. Results ①At concentration of 0.1,1,10,30 ?mol?L -1,propafenone attenuated DT from control (0.18?0.05) g to (0.14?0.03), (0.12?0.03), (0.08?0.02), (0.05?0.02) g respectively (P

It is important that the oral hospital establish a guarantee system of medical graduation clinical practice quality. Having experienced clinical education for many years,the hospital has formed a system in order to achieve objective management and process management organic unification,which can gradually promote the clinical practice quality.

[Objective]To report the records of 185 patients in whom severe scoliosis had been treated with preoperative halo-pelvic distraction and following total spine osteotomy and internal fixation.[Method]Preoperative halo-pelvic distraction was applied to all patients to reduce the severity of curvatures and make the following treatment possible.Then total spine osteotomy and internal fixation were performed to rectify the remained deformity of the spine.Halo-pelvic distraction was maintained during the operation and postoperatively to limit the mobilization of the patients.At one day after the operation,the patients were able to get out of bed,stand up and move around,making nursing care more convenient.[Result]Between 1983 and 2003,a total of 185 cases of severe scoliosis were treated by this approach and an average correction rate of 70.32% was achieved.Complete bone fusions were achieved at the cut ends of thc bone.The average loss of correction rate was less than 5 degrees.In the late stage of postoperative duration,rod release occurred in four cases and was resolved by second surgical intervention,showing no adverse effect on the clinical outcome.At one year postoperative infective complication occurred in one case and the patient fully recovered after the removal of the inserted device.X-ray examination confirmed good healing of the hone grafts.Root pain occurred in one case and the patient gradually recovered without any medical intervention.None of the rest patients had evidence of spinal cord or nervous system complications.[Conclusion]Halo-pelvic distraction instrumenation,in combination with total spine osteotomy,is an effective treatment modality for severe spinal curvatures.Halo-pelvic distraction can facilitate the operative procedure for those cases that can not be cured by application of single instrumentation or have difficulty in the insertion of the internal fixation device.The combined total spine osteotomy can further correct the spinal curves,reduce the burden of the fixafion device and avoid the occurrence of release or break down of the rods,eventually cure the severe scoliosis effectively.

[Objective]To study the mid-term outcomes about cementless total hip arthroplasty in the treatment of patients with alcoholic osteonecrosis of the femoral head.[Method]From March 1997 to June 2002,41patients(47 hips) was treatmented by cementless total hip arthroplasty,the mid-term (equal to or more than 5 years) results were evaluated both clinically and radiographically.Clinical outcomes were evaluated according to Harris evaluation score. Components migration,bone changes around the implant were measured radiologically. Kaplan-Meier analysis was performed to evaluate the surviving rate of the femoral and acetabulum components. End point was radiographical loosening or revision of the acetabular and femoral component for any reason.[Result]Forty-seven hips in 41 patients were followed up at least 5 years. The mean Harris hip score increased from preoperation(42.4?6.4)points to (91.8?4.4) points at the time of final follow up. No component was loose,No component was revised. The focal area of pelvic osteolysis in 2 hips and small focal area of femoral osteolysis in 6 hips were found. The surviving rate of the femoral and acetabulum components,was 1.0(95% confidence interval,0.98 to 1.00).[Conclusion]The mid-term outcomes about cementless total hip arthroplasty in the treatment of patients with alcoholic necrosis of femoral head had been satisfactory. Because the wear of component,osteolysis can not be avoided,clinical and radiographical follow-up for cementless total hip arthroplasty must be carried out persistently.

[Objeetive] To investigate the effect of minimally invasive V-shaped osteotomy instead of traditional operative technique for correction of ankylosing kyphosis.[Method]The C-arm X-ray was used under the local anaesthesia to select the space of osteotomy,the length of incision were 6~8 cm and only exposed one interlaminal space,the width of laminar V-shaped osteotomy were 8~10 mm.The operating table was changed from reverse V to type after finished osteotomy,the space of osteotomy would be automatica ly closed and replaced,if the space could not be automaticly closed,careful manipulation would be applied to close it.The bone mass of osteotomy was used in posterior bone graft of lamina.Postoperation,stric and standardized regulation were performed to send the patient back to ward.The fractional manual correction and external fixation of hyperextended plaster vest were performed respectivly 2 weeks after operation.[Result]Fifty patients of this group acquired more satisfied results except 1 case appeared recurrent deformity due to early removing of the plaster vest.[Conclusion]Minimal damage,lower medical expenses(without internal fixation)and satisfied results demonstrated the minimally invasive V-shaped osteotomy is a very effective method.It has advantages of Mutual intercalated V-shaped osteotomy space and better stability after reduction,posterior bone graft of lamina and external fixation of hyperextended plaster vest,therefore,all patients can acquire the bone graft fusion afer 6 months.

[Objective]To introduce the operative method and clinical experience of 200 cases with halo pelvic distraction and elastic growing rods fixation for treatment of scoliosis during growing period and comparison with pedicle screw systems. [Method]According to the principle of Harrington hook-rod system,inferior extremity was one hook hanged on the total lamina of lumbar vertebrae and superior extremity were two hooks hanged on the inferior articular process of thoracic vertebrae,rod I and rod II were connected by joint,between the end of rod I and joint set the spring cover for automatic extension followed spine growth.For the longer growing period children,the small incision could be performed between the hook and rod for fractional distraction to help the spine longitudinal grown until skeletal development and maturation.[Result]The average rate of correction of 200 cases with Scoliosis were 70.32% after 2~15 years follow-up survey,the height of the patients were increased by 5~22 cm and the preserved range of spinal motion was better than pedicle screw systems in long term follow up.Incidence of hook displacement was 2% and rod breaking was 3%.Reasons of hook displacement and rod breaking was related to unfitting installation and methods of internal fixation,incidence of hook displacement and rod breaking were decreased significantly post reform.[Conclusion]Halo pelvic distraction and elastic growing rods fixation for treatment of scoliosis during growing period which is a kind of biological method,does not affect the longitudinal growth of spine and prevent the complication of crankshaft phenomenon caused by pedicle screw systems.

[Objective]To sum up the experience of wearing the halo pelvic and level traction costoplasty to treat 35 cases of severe scoliosis combined with collapsed chest. [Methods]When scoliosis of patients were corrected to a certain degree after three weeks of halo pelvic distraction, the most serious collapsed ribs of concave side of the curve underwent a transverse incision the cross ribs. A special rib bender was used to bend the exposed 3 to 6 ribs. The bent ribs were pulled up by double-stranded 10# thick silk which was extended out through the skin and fixed in the upright post of the instrument of Halo pelvic distraction with rubber membranes.[Results]Thirty-three in 35 patients underwent costoplasty. The collapsed chest was turned round, respiratory function improved significantly and cyanosis of lips and nail bed disappeared. These offered advantages for next scoliosis correction.Two patients failed to undergo costoplasty due toprimary pulmonary atelectasis and pulmonary deficiency.[Conclusion]The instrument of Halo pelvic distraction is a good and convenient fixed point. Bending and turning round ribs can provide a convenient condition for recovery of collapsed chest. Halo pelvic distraction can be used not only for scoliosis correction but also for costoplasty.

0.05),and Salter pelvic osteotomy could significantly decrease the acetabular anteversion(P

Objective To assess the impact of recombinant pigment epithelium-derived factor (rPEDF) on the growth and invasion of the glioma cells.Methods The level of vascular enthothelial growth factor (VEGF) and thrombospondin-1 (TSP-1) were evaluated by enzyme-linked immunoadsorbent assay (ELISA); cell proliferation assays were performed by MTT assay; the cell migratory assay was used for evaluating its inhibitory migration rate.Results The VEGF expression (0.29?0.03) of A172_ PEDF (added the PEDF to the A_ 172 cells) decreased 4.4 times compared with the A172_ con (not added the PEDF to the A_ 172 cells, 1.31?0.02, P