Objective: In order to implement effectively on-scene medical response to a nuclear or radiological emergency, and also keep track of relevant on-scene audio and video information and monitoring data, we have designed and developed a monitoring platform for medical response to nuclear or radiological emergency. Methods:Based on relevant national standards for emergency response and the features of nuclear or radiological emergency, exploiting modern information technology and monitoring equipments, we formulate the design idea, characteristics, functions and framework of the platform. This platform takes treatment centers for nuclear or radiological injuries, designated medical institutions and medical response teams as main users. Results:We have developed the monitoring platform for medical response to nuclear or radiological emergency. Conclusion:Through the application of this platform, we hope to enhance the preparedness and on-scene response capability for nuclear or radiological emergency, and also provide information support for disposal of nuclear or radiological emergency in the future.

Objective To investigate the effects of the different concentration of mineral trioxide aggregate (MTA) on the proliferation and differentiation of dental pulp stem cells (DPSCs) from the young permanent teeth.Methods DPSCs were isolated from the young permanent teeth and cultured by tissue explant method.The expression of STRO-1 was detected by using immunofluorescence technology.DPSCs were cultured with different concentrations of MTA (0.02,0.20,2.00,20.00 g/L).Cell proliferation was detected by MTT array.Cells were cultured in the appropriate concentration of MTA for 4 weeks,and then stained by Alizarin red to detect their mineralized nodule formation capacity.The cells were cultured with the appropriate concentration of MTA and collected after 12,24,36,48 h.The mRNA expression of ALP,BSP,OC and DSP after the treatment of MTA were detected by quantitative PCR.Results DPSCs were positive for STRO-1.The capacity of 0.20 g/L MTA promoting the proliferation of DPSCs was stronger than other concentrations.After 4 weeks,the mineralized nodules of DPSCs were observed after alizarin red staining.The PCR showed that with increasing induction time,the expression levels of DSP and OC were up-regulated.But that of ALP and BSP was increased first and then decreased.Conclusions In this study,MTA can promote the proliferation of DPSCs at 0.02,0.20,2.00 g/L concentration.It can induce odontoblast differentiation effectively by 0.20 g/L MTA.

Recent studies have revealed that obease increases the risk of differentated thyroid carcino-ma (DTC);iodine increases the risk of papillary thyroid carcinoma wheras decreases the risk of follicular thy-roid carcinoma;vitamin D decreases the risk of DTC,but there are not enough evidences to prove that vitamin A,C and E can decrease the risk of DTC;alcohol may decrease the risk of DTC,but the mechanism is still un-known;xenobiotics increases the risk of DTC.It is essential for DTC prevention to clarify the protection and risk factors.

0.10). Conclusion: Donepezil is effective in treating the cognition of AD and some of the mixed dementia, but it has little effect on VD.

Objective To study the effect of rehabil it ation training on the expression of S-100, GFAP and Nestin in the cortex of cer ebrally infarcted rats. Methods Sixty Sprague-Dawle y rats were used. Experimental cerebral infarction was caused in all the rat s. 24 hours after the infarction, all the rats were randomly divided into 3 grou ps: a rehabilitation group, an immobiliaztion group and a control group. The rat s in the rehabilitation group were given balancing, grasping, rotating and walki ng exercises everyday, while those in the immobilization group were immobilized in cages. The rats in the control group was without any treatment. Immunohistoc hemical technique was used to detect the S-100, GFAP and Nestin expression at t he time points of 24 hours, 1, 2, 3 and 4 weeks after infarction, respectively. Results S-100, GFAP and Nestin expression were foun d around the infarcted cortex. The response increased as the extension of the su rviving time. The immunohistochemical reaction in rats in the rehabilitation gro up was stronger than that in the immobilization group. Conclusi on Rehabilitation training can activate astrocytes, which could impr ove the internal environment, protect the neurons and promote repairing of neuro n.

Objective The study evaluated the efficiency and safety of a short-acting full-dose fibfinolytic regimen to promote early infarct-related artery (IRA) patency during the inherent delay experienced by infarction patients referred for angioplasty as the principal recanalization modality. Method Accepted aspirin and heparin, 75 patients were treated by an intravenous bolus of 20 mg recombined tissue-type plasminogen activator (rt-PA), then followed by 80 mg over 30 minutes. Patients were to undergo angiography as soon as possible following study drug. If the IRA was occlude (TIMI flow grade 0 or 1),or was open but with ≥50% stenosis, immediate early PTCA at the same time. The end point included patency rates on catheterzation laboratory (cath Lab) arrival, technical results when PTCA was performed, complication rates, clinical adverse events rates and left ventricular (LV) function by treatment assignment in two weeks. Results Patency on Cath lab arrival was 88% with combination treatment group (26% Thrombolysis in myocardia infarction trial TIMI-2, 66%TIMI-3), and 36%with primary PTCA group (20%TIMI-2, 16%TIMI-3), (P

[Objective]To investigate the effect of transforming growth factor ?1(TGF-?1) genes transfection on the proliferation and differentiation of chondrocyte by adenovirus vector,and to observe the quality in repair cartilage defect through constructing tissue-engineered cartilage.[Method]Using replicat ion defective adenovirus Adeno-X~(TM) as a carrier,Articular chondrocytes were transferred by high titers level recombinant adenovirus taking TGF-?1 genes.Ultromicrostructure,prolife ration anddifferentiation were observed by light and electron microscope,flow cytometry and so on.Induce expression of exogenous genes coding protein and cartilage cell phenotype were detected by Immunocytochemistry and Northern blot.Gross observation,histology and effect classification were used to evaluate results in repair cartilage defect by combi nation of infected chondrocytes and bone matrix gelatin(BMG).[Result]Immunocytochemistric staining showedthe expression of exogenous gene coding proteins after infection,and procollagen Ⅱ mRNA in cells was detected increasingly by Northern blot.TGF-?1 stimulated proliferation of chondrocytes in primary cultured,and resulted in recruitment of articular chondrocytes into S-G2/M phase.Chondrocytes seeded onto BMG showed high level of proliferation.Transplantation in vivo showed: cartilage defect was repaired satisfactorily by Combination of BMG and infected chondrocytes,and the structure of repair tissue got close to normal articular cartilage.[Conclusion] Articular chondrocytes prolifera tion could be stimulated by adenovirus vector taking TGF-?1 genes,which could be used to constructed tissue-engineered cartilage,and applied in repairing joint cartilage defect.

Adult ; Antihypertensive Agents ; therapeutic use ; Blood Pressure ; drug effects ; Depression ; complications ; drug therapy ; physiopathology ; Drug Therapy, Combination ; Drugs, Chinese Herbal ; therapeutic use ; Female ; Humans ; Hypertension ; complications ; drug therapy ; physiopathology ; Male ; Middle Aged ; Phytotherapy ; Powders ; Treatment Outcome

The usage of Chinese materia medica (CMM) is a key scientific problem on its safety and efficacy in clinical application. The daily administration time is one of the main issues and contents of CMM usage, which is directly related to the clinical efficacy. But, it is hardly to find the research aiming at the rationality of daily administration times of CMM. To achieve new breakthrough in research on daily administration times of CMM, one research presumption is put forward for the first time and it is the evaluation pattern for the rationality of daily administration times of CMM based on pharmacodynamics-pharmacokinetics. The further purpose is to supply innovative and practical research thought and method for scientifically confirming the daily administration times of CMM and enhancing its clinical efficacy.

Objective To obtained the gene AcPKS1 of Antrodia camphorata, analyze using bioinformatics, and detect the condition of expressing in the different medium. Methods Polyketide synthases gene was obtained from the genome of A. camphorata through analyzing the genome, and the full length of the gene was obtained through designed the special primers including initiation codon and termination codon and the template using cDNA of A. camphorata, which named the gene AcPKS1, and using the bioinformatics analysis and expression profiles analysis in the different medium. Results The full length of AcPKS1 gene was 6 348 bp, including six introns and seven exons, and the expression region encoded 2 115 amino acids; the bioinformatics analysis showed that AcPKS1 was a kind of nonreduced PKS of type in fungi, the domains was SAT-KS-PT-ACP-ACP-TE, and the enzyme catalyzed a new kind of cyclization in the process of polyketides biosynthesis; The expression profiles revealed that glucose was necessary during the expression of AcPKS protein, and the expression quantity of the AcPKS1 protein basically proportion to the content of glucose. Conclusion The result of this text has applied foundation to identify the polyketide synthase gene and take full advantage genomic resources of A. camphorata.