The treatment of relapsed and refractory multiple myeloma has always been a problem among hematologic malignant tumor treatment.Compared with the initial therapy,there are worse therapeutic effects and more affecting factors of selecting treatment since the existence of drug resistance in relapsed myeloma.In this review,the therapeutic effects of single and combinational use of several novel agents such as thalidomide,lenalidomide and bortezomib were evaluated in treating relapsed and refractory multiple myeloma.The clinical results of some new anti-myeloma agents were also reported.

The incidence of venous thromboembolism (VTE) in multiple myeloma patients is higher than that of normal population. In this review, recent advances about thrombosis in multiple myeloma are studied,including the mechanism,risk factors,therapy and prevention of thrombosis.

Multiple myeloma (MM) is a heterogeneous disease.The prognosis of MM varied a lot among different patients.One of the main basis of risk stratification is genetic abnormality,which including the number or structure anomaly of chromosomes,genetic aberrations,signaling pathway disorders and epigenetic changes.The risk stratification is being revised with the development of FISH,gene-expression profiling and next-generation sequencing.These combined approaches would hopefully transform the current one-for-all care into a more tailored,individual therapeutic strategy for MM patients and would finally improve their quality of lives.

1 case of intraductal papilloma of parotid gland was analyzed by means of clinicopathologic data,hematoxylin-eosin and immuno-histochemical staining.Combined with the relevant literature,clinical pathological features,diagnosis and treatment of the salivary gland intra-ductal papilloma were discussed.

BACKGROUND:Platelet-rich plasma gel contains some kinds of growth factors, which play important roles in repair of local trauma and bone defects. Platelet-rich plasma gel is now more and more studied as tissue engineering scaffolds, but there are no unified preparation methods.
OBJECTIVE:To summarize the studies about the preparation methods of platelet-rich plasma gel and the growth factors in platelet-rich plasma gel in recent years, and to compare different methods in order to find which one is better.
METHODS:A online search of PubMed database from January 1980 to January 2013 and China National Knowledge Infrastructure from January 1980 to January 2013 was performed with the key words of“platelet-rich plasma, platelet-rich plasma gel, tissue engineering”in English and Chinese, respectively. The studies about detection and preparation methods of platelet-rich plasma were included and those unrelated to platelet-rich plasma were excluded. Final y, 322 articles were gained and 67 articles were included in result analysis.
RESULTS AND CONCLUSION:Al preparation methods have their advantages. We should select the suitable method according to the goal of study. With the further study of platelet-rich plasma, it wil become more and more important for bone defect repair and tissue engineering research.

Tissue factor (TF), a transmembrane glycoprotein with a molecular mass of 47000, plays an important role of coagulation within vessel wall. Recently, people also present that TF have identifying and regulating function in cells signaling. And it contributes in the pathogenesis of atherosclerosis, angiogenesis and restenosis. This review presents the development of TF in above mentioned .

A simple, rapid and accurate method is described for the determination of icariin in Fuchun Tablets by reversed-phase high-efficiency liquid chromatography. The samples were first extracted with 70% alcohol for 50 minutes The resulting solutions were chromatographed on aYWG-C18 column.By using a mobile phase of methanoltetrahydrofuran-water (26.5:15.9:56.7) and a detection wavelength at 274 nm, the recovery for 101.02%, CV=2.4%,are obtained.The method can be employed for the quality control of Fuchun Tablets.

[Objective]To study the possibility of differentiation of rabbit marrow mesenchymal stem cells(MSCs)into endothelial cells in vitro.[Method]BMSCs of rabbits were obtained by using gradient centrifuge method.The adhesive cells were preserved to passage culture to get pure MSCs.MSCs in the second generation were used.After induction with vascular endothelial growth factor(VEGF),microscopic exarm,cytoimmunofluorescent staining confirmd the trial group cells becomes to endothelial cells.W-P corpuscles which was the symbol of endothelial cells were observed by transmission electron microscope.[Result]The differentiated cells demonstrated the characters of endothelial cells under phase contrast microscopy.The cytoimmunofluore-scent staining showed that the trial group cells were induced to endothelial cells.W-P corpuscles could be seen under transmission electron microscope.[Conclusion]The adult rabbit BMSCs can present features of endothelial cells in certain induction medium,they are ideal donor cells of tissue engineering vascularization.

Applying H-index combined with other evaluation index of qualitative and quantitative evaluation for seientifie research ability, levels of researchers as well as academie journals, is becoming inereasingly concerned and will gradually be applied. The paper discusses the connotation, extension, feasibility, limitations on H-index and further analyzes the applicability of using H-index to evaluate the quality of academic journals.

BACKGROUND: Schwann cells are the only glial cells in the peripheral nervous system and play an important role in the regeneration of peripheral nerves, while they have poor proliferation capacity and decreased activity, need allografts, and are difficult to culture in vitro. OBJECTIVE: To analyze the feasibility of repairing neurologic defects with tissue-engineered nerves constructed with Schwann cells derived from bone marrow stromal stem cells. DESIGN, TIME AND SETTING: Randomized controlled observation was conducted at Heilongiiang Institute of Veterinary Pharmaceutics between March 2004 and April 2005.MATERIALS: Twenty-four 8-weck-old female Wistar rats were used to establish animal models with 10 nun defect of sciatic nerve. METHODS: Twenty-four rats were divided into 3 groups by random digits table, tissue-engineered nerve group, polylactic acid robe group and autologons nerve group, with 8 rats in each group. Tissue-engineered nerve group: tissue-engineered nerve Was used to bridge neurologic defect, which was constructed with Schwann cells derived from bone marrow stromal stem cells, natural extracelhilar matrix gel and degradable polylactic acid tube. Polylactic acid robe group: injecting extracellular matrix gel into degradable polylactic acid tube to bridge neurologic defect. Autologous nerve group: 10 mm of nerve was cut and performed end-to-end anastomosis after revolving 180 degrees. MAIN OUTCOME MEASURES: Functional recovery of sciatic nerve was detected with electrophysiological observation of nerve, and histological observation and axon count of the newly generated nerve tissue at 12 weeks after transplantation. RESULTS: After introduction, adult bone marrow stromal stem cells had the morphology and properties of Schwann cells. The regenerated nerve had grown to the distal end passing through the defect at 12 weeks after transplantation. The detection indexes in the tissue-engineered nerve group and autologous nerve group were better than that in the polylactic acid tube group (P < 0.05), there were no significant differences between the tissue-engineered nerve group and autoiogous nerve group (P < 0.05); The degradation and absorption of polylactic acid tube were obvious in the tissue-engineered nerve group and autologous nerve group. CONCLUSION: Human bone marrow stromal stem cells can be induced to differentiate into Schwann cells in vitro, and tissue-engineered nerve constructed with Schwann cells, extracellular matrix and degradable polylactic acid tube can repair peripheral neurologic defect.