1.Analysis of Measurement Uncertainty in Determination of Irbesartan Tablets by HPLC
China Pharmacist 2014;(2):326-328
Objective:To find out the impacting factors in uncertainty by analyzing the measurement uncertainty in the determina-tion of irbesartan tablets by HPLC and provide the evidence for the measurement evaluation. Methods:A mathematic model for calcu-lating uncertainty was established, and every component in the uncertainty was assessed. Results:The expanded standard uncertainty for the HPLC determination was 2. 4%. Conclusion:The measurement uncertainty of the experiment is mainly caused by the measur-ing method.
2.Effect of video-assisted thoracoscopic surgery in treatment of hemopneumothorax
Changjiang LI ; Qidong YUAN ; Xiangchao CHENG
Chinese Journal of Primary Medicine and Pharmacy 2010;17(16):2163-2164
Objective To investigate the therapeutic efficacy of Video-assisted thoracoscopic surgery (VATS)in treating hemopneumothorax. Methods 83 cases of hemopneumothorax treated with exploratory thoracotomy with the help of VATS were retrospectively analyzed. Results All the 83 cases were completed successfully by the VATS.22 cases were treated with bunamiody resection,42 eases were treated with lung repair operation,12 cases were treated with pleuropexy,5 cases were treated with albumingel injection,2 cases were treated with thoracotomy because of serious injury.All patients were followed up 1 year and were found in good condition. Conclusion VATS could treat bunamiody resection and lung repair operation,can stop the bleeding effectively, cause less injury,good therapeutic effect and quickly recovery.
3.Diagnosis and surgical treatment of retrosternal goiter
Changjiang LI ; Qidong YUAN ; Xiangchao CHENG
Chinese Journal of Primary Medicine and Pharmacy 2010;17(13):1755-1756
Objective To observe the diagnosis and surgical treatment of substemal goiter. Methods The clinical data of 26 cases with substemal goiter underwent thyroidresection were retrospectively analyzed. Results All the 26 cases were treated by operation. 20 cases were operated by Lower collar incision. 6 cases were operated by ster-notomy. The pathological results showed nodular goiter in 18 cases,follicular adenoma in 6 cases,and papillary carcinoma in 2 cases. Conclusions Surgery via cervical collar incision was fit for most of retrostemal thyroid nodules,and sternotomy or thoracotoray were safely fit for large or carcinomatous substemal goiter. Most cases of retrostemal goiter could be removed by Lower collar incision.
5.Research on quality testing for active spot scanning proton and heavy ion accelerator
Jinsheng CHENG ; Jilong YUAN ; Mingsheng LI
Chinese Journal of Radiological Medicine and Protection 2016;36(8):621-625
Objective To study the quality testing of dose delivery system of the active spot scanning proton and heavy ion accelerator,in order to provide the reference for the quality control of related equipment.Methods In the four therapy rooms,both 0.6 cc chambers and Gafchromic EBT3 films were used,respectively,to test the accelerator for dose reproducibility,dose linearity,dose stability,depth dose distribution,beam scanning position deviation and radiation field uniformity in each therapy room.Results Dose reproducibility variation coefficients are all less than 1.5%,dose linearity's maximum deviations less than 2%,dose stability's deviations less than 2%,depth dose distribution stability within 2%,beam scanning position deviation less than 1 mm,consistency of irradiation field's deviation less than 2 mm,and flatness within ± 5%.Conclusions The indicators about quality testing for the active spot scanning proton and heavy ion accelerator are all in line with the requirements of IEC standards draft.
6.Quality control research of active spot scanning proton and heavy ion accelerator's radiation field uniformity
Jilong YUAN ; Mingsheng LI ; Jinsheng CHENG
Chinese Journal of Radiological Medicine and Protection 2016;36(8):626-629
Objective To develop the method for testing the consistency of irradiation field produced by the active spot scanning proton and heavy ion accelerator.Methods Calibration of the EBT3 films were carried out with the calibrated ion beam to establish the dose calibration curve.According to the different proton and carbon ion energies (proton:94.29,150.68,212.62 McV;carbon ion:175.99,283.43,412.54 MeV/u),EBT3 films were located in the solid water phantoms in each therapy room,respectively.Finally,the irradiated EBT3 films were scanned and the radiation field size's deviation and flatness were analyzed.Results In different conditions,radiation field size's deviations were all less than 2 mm and the flatness parameters were all controlled below the 5%.Conclusions EBT3 films can be used to test the active spot scanning proton and heavy ion accelerator's radiation field uniformity.
7.Dynamic Effect Analysis on the Gap of Different Types of Health between Urban and Rural Medical and Health Resource Allocation:Further Investigation Based on State Space Model
Lin YANG ; Qian CHENG ; Yuan LI
Chinese Health Economics 2014;(7):33-36
To investigate different effects of fiscal health expenditure, household spending on health and social health expenditure on narrowing the gap between urban and rural health resource allocation. Methods: With the relevant data of China’ s medical and health through 1985-2011 years, taking methodology of the state space model to estimate the varying-time elasticity of different types of expenditures on urban and rural health resource allocation gap. Results: For narrowing the gap, household health expenditure played the leading role, fiscal health expenditure played smaller role and the social health expenditure played the supplementary role; the elastic of different health expenditure proportion was fluctuated before 2002, which became stable after 2002; it is easy to improve the “hard conditions” rather than the “soft conditions” . Conclusion: To accelerate the process of urban and rural medical security system integration, it is inevitable to establish an efficient configuration mechanism for urban and rural health expense, balanced develop urban and rural medical insurance system and scientifically guide social health investment.
8.The influence on the quantity of the template DNA on the results of the automatic fluerescent detection of STR multiplex amplification
Lihua GU ; Yuan PING ; Li CHENG
Chinese Journal of Forensic Medicine 1986;0(02):-
Objective For the purpose of exploring the influence exerued by the amount of template DNA on the results of automatic fluerescent detection. Method For the purpose of buiding the optimal amount of template DNA amplified, we have analyzed different amount of template DNA (9947A) by 3100 Genetic Analyzer with the multiplexing Profiler Plus Kit. Results The optimal amounts of template DNA amplified were between 0.31ng and 2. 5ng by using 3100 Genetic Analyzer. Conclusion It will produce the incorrect analytic results if the amounts of template DNA were too high or too low.
9.The expression of nitric oxide synthase and its association with proliferation and apoptosis of HCC cells
Tianming CHENG ; Aili YUAN ; Chaolong LI
Chinese Journal of Digestion 1996;0(05):-
Objective This study was to investigate the relation of nitric oxide synthase with cell proliferation and apoptosis in hepatocellular carcinoma (HCC). Methods The expression and localization of inducible nitric oxide synthase (iNOS) and PCNA in 21 patients with HCC were studied by immunohistochemistry. Apoptotic cell in tissue section was detected by TUNEL (terminal deoxynucleotidyl transferase [TdT] mediated desoxyuridinetriphosphate [dUTP] nick end labeling). Results The iNOS protein was markedly expressed in the liver tissue of hepatic cirrhosis or chronic liver hepatitis, which was close to tumor edge. Meanwhile it was weakly or undetectable in the liver tissue localized beyond 1.5 cm from the tumor edge. The iNOS protein was markedly expressed at the invasive tumor edge and HCC cells that invased into stroma, but it was weakly or undetectable at the most tumor core. The expression of iNOS was correlated with the proliferative activity of cells, and the number of apoptotic cells was correlated to the number of PCNA positive cells ( P
10.Prokaryotic expression and bioinformatics analysis of NS5A transactivating protein 7 of hepatitis C virus
Xiaoguang LI ; Jun CHENG ; Yuan HONG
Medical Journal of Chinese People's Liberation Army 2001;0(10):-
Objective To construct prokaryotic expression vector of NS5A transactivating protein 7 of hepatitis C virus (NS5ATP7), induce the expression of NS5ATP7 in E. coli, and to predict its structure and function by bioinformatics analysis. Methods NS5ATP7 gene was amplified by reverse transcription PCR (RT-PCR) using HepG2 cells mRNA as template, and was ligated into pGEM-T cloning vector. After verifying the sequence of the inserted fragment by restriction enzyme digestion identification and sequencing, NS5ATP7 was cloned into inducible prokaryotic expression vector pET-32a(+) and transfected into competent E. coli BL21. The protein expression was induced with IPTG and the product was analyzed by SDS-PAGE and Western blotting hybridization. The structure and function of NS5ATP7 were predicted using bioinformatics techniques. Results NS5ATP7 gene with about 891bp was amplified by RT-PCR, which was coincident with that we expected, and it was then inserted into expression vector pET-32a(+). Under the induction of IPTG, the recombinant NS5ATP7 was expressed successfully. SDS-PAGE and Western blotting assay showed that this recombinant protein possessed good immunogenicity. Bioinformatics method such as ExPASy, TMHMM and SignalP analysis indicated that NS5ATP7 was full of helices, had neither transmembranous structure nor signal peptide. Conclusions The recombinant NS5ATP7 gene could be successfully expressed in prokaryotic expression system of E. coli, which might lay the foundation of studying the immunogenicity and biological charactersitics of the NS5ATP7. Bioinformatics analysis may provide a new method to analyze the structure and function of a new protein.