Objective To study the effect of finasteride on preventing prostate hyperplasia with acute urinary retention.Methods Totally 626 patients with benign prostatic hyperplasia (BPH) were randomly divided into treatment group (316 cases) and control group (310 cases),followed up for 3 years.The incidence of acute urinary retention and prostate volume changes were analyzed.Results There were 3 cases of acute urinary retention in treatment group (1.0 ％),9 cases in control group (2.9％) (x2 ＝5.37,P＜0.05).In treatment group,the average prostate volume was reduced about 23.6％ compared with before taking the medicine,on the contrary,prostate volume was averagely increased 12.3％ in control group.Conclusions Finasteride can reduce the risk of acute urinary retention by narrowing the prostate volume in patients of benign prostatic hyperplasia.

ObjectiveTo study the feasibility,safety and efficacy of laparoscopic cyst excision and Roux-en-Y hepaticojejunostomy for adult congenital choledochal cyst.MethodA retrospective study was conducted on 7 adult patients with congenital choledochal cyst who received total laparoscopic cyst excision and Roux-en-y hepaticojejunostomy from May 2008 to February 2011 in the Department of General Surgery of Shengjing Hospital,China Medical University.ResultsAll the laparoscopic surgery was successful.The mean operation time was 210 minutes.The average intraoperative blood loss was 80 ml.All patients were out of bed within the first 24 h after surgery.The mean time to first flatus/bowel motion was 2.4 days.Except 1 patient who had small amount of bile leakage,all patients recovered smoothly without any major postoperative complications.The average hospital stay was 8.1 days.No patients suffered from abdominal pain,fever or jaundice during follow-up from 3 to 30 months.ConclusionTotal laparoscopic cyst excision and Roux-en-Y hepaticojejunostomy was a safe,efficacious,and minimally invasive procedure.

AIM: To determine whether Sca-1~+ cells from fetal liver can differentiate into neural cells. METHODS: The sex of 14.5-day-old murine fetuses was determined by PCR analysis of sry gene, and Sca-1~+ cells from male fetal liver were isolated with a magnetic cell sorting kit, 2?10~3 of which were then transplanted into lethally irradiated female mice. The donor cells and their characteristics in recipient brains were identified and detected by FISH and immunohistochemistry double-staining analysis at 60, 120, 180 days after transplantation. RESULTS: There existed many male cells in brains of female recipients, some of them express neuron-specific nuclear protein (NeuN), and some of them express the astrocyte-specific marker, i.e. glial fibrillary acidic protein (GFAP). CONCLUSION: Sca-1~+ cells from fetal liver, which contain hematopoietic stem cells, can differentiate into neuronal cells and astrocytes in the brains of adult mice.

Objective To optimize the purification technology of total flavonoids from the Tripterospermi Chinensis Herba by macroporous resin. Methods The purification abilities of six kinds of macroporous adsorption resins for total flavonoids from Tripterospermi Chinensis Herba were studied with the adsorption and desorption rates as the index by static adsorption and desorption experiments. The column liquid concentration, adsorption rate, and the loaded amount were studied by using dynamic adsorption experiments. The optimal desorption technology was examined by orthogonal experiment. Results AB-8 macroporous resin was found to have good adsorption and desorption effects. The optimal purification conditions were as follows:the column liquid concentration was 5.285 mg/mL, adsorption rate was 2 BV/h;the loaded amount was 17.62 mg/mL. The sample was eluted by 10%ethanol with 4 BV and 50%ethanol of 5 BV at a flow rate of 4 BV/h. The purity of total flavonoids increased to 61.95% after the purification, and the yield was 87.28%. Conclusion This optimized process was stable, feasible and suitable for separation and purification of total flavonoids from Tripterospermi Chinensis Herba.

Objective To explore the method and effects of operative treatment of superior shoul-der suspensory complex(SSSC)injury.Methods A total of 18 patients with SSSC injury were enrolled. There were six cases of scapular neck fracture combined with comminuted clavicle fracture,five cases of scapular neck fracture combined with acromial fracture,and two cases of comminuted clavicle fracture com-bined with acromial and glenoid fracture.These patients were treated with open reduction and internal fixa-tion.Three patients had acromioclavicular joint dislocation combined with coracoclavicular ligament rupture and they were given hook plate fixation and ligament repair.One patient with scapular neck fracture com-bined with acromioclavicular joint dislocation,and one patient with comminuted clavicle fracture combined with coracoclavicular ligament rupture,were treated with the open reduction,internal fixation and the liga-ment repair.Results All patients were followed up for 3 to 14 months,with an average of 9 months.Ana-tomical reduction and healing were all achieved within 7 to 12 weeks,with an average of 8.6 weeks.Con-clusion Anatomical structure of SSSC is very complex and surgery is given priority to internal fixation, which restores the stability good therapeutic effect.

In the tumor microenvironment, inflammatory cells regulate intracellular signaling transduction mediated by the corresponding nuclear transcription factor by releasing inflammatory factors ,which leads to transformation of epithelial cells to mesenchymalcellsand eventually promote tumor metastasis. Because of the intimate relationship between inflammation and tumor metastasis , it would be helpful for further enhancement of effectiveness of tumor therapy if we could better understand both the biological character of tumor and the related inflammatory factors.

This paper presents a feature extraction method based on multivariate empirical mode decomposition (MEMD) combining with the power spectrum feature, and the method aims at the non-stationary electroencephalogram (EEG) or magnetoencephalogram (MEG) signal in brain-computer interface (BCI) system. Firstly, we utilized MEMD algorithm to decompose multichannel brain signals into a series of multiple intrinsic mode function (IMF), which was proximate stationary and with multi-scale. Then we extracted and reduced the power characteristic from each IMF to a lower dimensions using principal component analysis (PCA). Finally, we classified the motor imagery tasks by linear discriminant analysis classifier. The experimental verification showed that the correct recognition rates of the two-class and four-class tasks of the BCI competition III and competition IV reached 92.0% and 46.2%, respectively, which were superior to the winner of the BCI competition. The experimental proved that the proposed method was reasonably effective and stable and it would provide a new way for feature extraction.
Algorithms ; Brain ; physiology ; Brain-Computer Interfaces ; Discriminant Analysis ; Electroencephalography ; Humans ; Magnetoencephalography ; Principal Component Analysis

Objectives To study the suicide attempts and suicide attitude status and the characteristics of Medical University undergraduates, provide specific recommendations for mental health education and counseling. Methods The investigation was conducted among 567 undergraduates and 130 graduate students in Kunming Medical University with Self-designed Suicide Attempts Investigation and Suicide Attitude Questionnaire (QSA), and the results of different groups were compared. Results 14.46%undergraduates had suicidal ideation, 4.23%undergraduates had suicide attempts, but there were no significant different between female and male. The suicide attitude in general was contradictory and neutral. Sex,academic qualification,grade, family location and economic status variables had influence on suicide attitude. Conclusions Although the medical university undergraduates disapproved of the suicidal behavior, but it still reflected the pursuit and respect for the humanistic concern and value of life to a certain extent.Suicidal attitude had significant different in demographic variables.

Aim To establish a screening system of orphan G protein-coupled receptors (oGPCRs) for their ligands based on monitoring [Ca 2+]_i in engineered cells. Methods The whole ORF of a member of human oGPCR, designated human G-protein-coupled receptor c (hGPCRc), was amplified by RT-PCR from human colon tissue and its structure was analyzed with softwares. CHO-K_1 cells were transfected with the recombinant pcDNA 3.1(+)-hGPCRc to obtain engineered CHO-hGPCRc cells. As fluorescence probe, Fluo-3 was used in assaying the [Ca 2+]_i changes induced by different compounds in the CHO- hGPCRc cells.Results Bioinformatic analysis showed that hGPCRc was localized at 13q32.2, and its corresponding amino acids formed seven-transmembrane domains and was close to human P2Y_1 receptor. It was indicated that hGPCRc was a new member of human GPCR. CHO- hGPCRc cells expressing hGPCRc were obtained successfully but no one was able to activate hGPCRc among the tested compounds indicated by the [Ca 2+]_i changes. Conclusion Although hGPCRc was even though close to human P2Y_1 receptor, it can not be activated by the known compounds which activate the P2Y_1 receptor. hGPCRc might be a new member of purine receptor family but dose not belong to P2Y_1 subfamily.

AIM:To analyze and compare the chemical constituents difference between single medicine decoction(SMD) and mixed medicine decoction(MMD) of Changweikang Granules. METHODS: TLC and HPLC methods were applied to comparing the chemical constituents in SMD and MMD of Changweikang. RESULTS: Spots of TLC between SMD and MMD similar,except for some difference in the HPLC. CONCLUSION: The analyses were carried out for the first time.We found that the plus of the chemical constituents in SMD equal to MMD of Changweikang,also during the course of MMD,new constituents may be generated.Based on these,we proposed that the material base of Changweikang were the rational preparation and complementation of the effective components and the generation of new constituents.