Objective To study the effect of cumulus cell apoptosis on the structure of human oocyte cultured in vitro and to probe into the reason why cumulus cell apoptosis rate can be used clinically to predict the developmental potential of oocyte. Methods Cumulus-enclosed oocyte complexes (COCs) at the GV stage were cultured in vitro. The apoptosis rate of cumulus cells for every COC was evaluated with HE staining, DAPI staining and TUNEL labeling methods. Oocytes were divided into two groups according to their cumulus cell apoptosis rate. Structure of oocytes was observed under light microscope and transmission electron microscope. Mature oocytes at MⅡ stage were fertilized and cultured in vitro for two days. Then the embryos were evaluated according to their morphology under light microscope.Results The oocytes with low cumulus cell apoptosis rate had a normal structure and a promising developmental potential, while the oocytes with high cumulus cell apoptosis rate had deformed structures and a poor developmental potential. Some deformed oocytes had uneven perivitelline space. For oocytes with a small perivitelline space, the organelle development was slow compared with those with a large perivitelline space. Some had unevenly piled organelles. Secondary lysosomes and large space probably caused by the decopmounding of lysosomes were found in the ooplasm of these oocytes. The deformed oocytes appeared to have lots of swollen mitochondria with blurred cristae and membrane. Some of these oocytes had fractured first polar bodies and abnormally thick or thin zona pellucida. The cell junctions and microvilli of cumulus cells reduced as well. Conclusion The effect of cumulus cell apoptosis on the structure of oocyte cultured in vitro was revealed. The reason why cumulus cell apoptosis rate prognosticated the developmental potential of oocyte was demonstrated in the aspect of oocyte structure.

Objective To analyze the clinical features of the multiple trauma patients combined with spine and spinal cord injuries.Methods A retrospective study was performed in 143 multiple trauma patients combined with spine and spinal cord injuries admitted to our department between March 2004 and March 2009.The parameters including injury cause,segment of injuries,associated injuries,complications,treatment methods and outcomes were analyzed.Results Falling and traffic accidents were the main causes for the injuries of spine and spinal cord,accounting for 53.8%(77 cases)and 38.5%(55 cases),respectively.The injured segments involved 101 lumbar vertebrae(50.8%),61 thoracic vertebrae(30.7%),29 cervical spines(14.6%)and 8 sacrococcygeal vertebrae(4.0%).The associated injuries were located at chest(163 regions,38.6%),abdomen(84 regions,19.9%),head and neck(77 regions,18.3%),extremity(65 regions,15.4%),face(17 regions,4.0%)and body surface(16 regions,3.8%).The early complications included electrolyte disturbances in 33 patients (16.8%),respiratory infection in 30(15.3%)and abdominal distention in 19(9.7%).The late complications were malnutrition in 26 patients(13.3%),amyotrophy in 23(11.7%)and deep vein thrombus in 11(5.6%).Treatment methods were operations and expectant treatments in 106 patients (74.1%)and 37(25.9%)respectively.According to American Spinal Injury Association(ASIA)scale,there were 20 patients(14.0%)at grade E before treatment and 53(37.1%)at grade E after treatment.Of all,12 patients were died of mainly multiple organ failure(MOF),cerebral hernia and malnutrition,with mortality rate of 8.39%.There showed an increase of complication and mortality rate with increase of ASIA grade(P ＜ 0.05).Conclusions The spine and spinal cord injuries in patients with multiple trauma are mainly caused by high energy injuries and characterized by high injury severity,complex associated injuries,multiple complications,difficult management and high mortality rate.

OBJECTIVETo provide further research with literature evidence of the variety origin, traditional effect and dosage.

METHODTextual research on medicinal works of past dynasties.

RESULTThe origin plants are Pueraria lobata, P. thomosonii and P. eduli. Its present effect is the same as ancient literature. However the fresh juice of the plant used for the treatment of bitter by mad dog and aborticide as literature recorded should be proved by further researches. The dosage is different among past dynasties.

CONCLUSIONAs one of the often used Chinese materia medica, Gegen has a bright prospect.

Anti-Inflammatory Agents, Non-Steroidal ; isolation & purification ; therapeutic use ; Antidiarrheals ; isolation & purification ; therapeutic use ; Drugs, Chinese Herbal ; isolation & purification ; therapeutic use ; Fever ; drug therapy ; Humans ; Phytotherapy ; methods ; trends ; Plants, Medicinal ; chemistry ; classification ; Pueraria ; chemistry ; classification

The definition and management of microinvasive cervical cancer varies from time to time depending on the organization involved and is a persistent focus of controversy. The purpose of defining microinvasion is to identify a group of patients who are not at risk of lymph node metastases or recurrence and who therefore may be treated with less than radical therapy. Microinvasive cervical cancer with Humans ; Hysterectomy ; Lymph Node Excision ; Lymph Nodes* ; Neoplasm Metastasis* ; Recurrence ; Uterine Cervical Neoplasms

OBJECTIVETo explore the clinical effects of surgical treatment with cable dragged reduction and cantilever beam internal fixation by posterior approach for odontoid fracture associated with atlantoaxial dislocation.

METHODSThe clinical data of 12 patients with odontoid fracture associated with atlantoaxial dislocation from January 2008 to December 2013 were retrospectively analyzed. There were 8 males and 4 females, ranging in age from 21 to 53 years with an average of 37.2 years. Eleven cases were fresh fracture and 1 case was old fracture, all patients complicated with atlantoaxial anterior dislocation. According to Anderson-D' Alonzo typing method modified by Grauer, 3 cases were type IIA, 5 cases were type IIB, 3 cases were type IIC, and 1 case was type IIIA. All patients underwent surgical treatment with cable dragged reduction and cantilever beam internal fixation by posterior approach. JOA score and ADI method were respectively used to evaluate the nerve function and reductive condition of atlantoaxial dislocation.

RESULTSAll patients were followed up from 6 months to 2 years with an average of 1 year and 3 months. At 1 week, 6 months after operation, and final follow up, JOA scores were 13.2±1.3, 13.5±1.4, 14.3±1.5, respectively, and these data were obviously better than that of preoperative 8.3±1.4(<0.05). Postoperative X rays and CT showed satisfactory reduction of atlantoaxial dislocation. At 1 week, 6 months after operation, and final follow up, ADI were (2.2±0.4), (2.4±0.6), (2.3±0.5) mm, respectively, and these data were obviously better than that of preoperative.(5.8±1.2) mm(<0.05). All screws and cables had good location without looseness and breakage, and bone graft got fusion.

CONCLUSIONSSurgical treatment with cable dragged reduction and cantilever beam internal fixation by posterior approach for odontoid fracture associated with atlantoaxial dislocation is a good method, with advantage of firm fixation and high safety. It could obtain good clinical effects.

［摘要］ 目的：探讨miR-183 对脑胶质瘤细胞放射敏感性的影响。方法：2020 年10 月至2021 年6 月，收集秦皇岛市第一 医院40 例脑胶质瘤组织标本，对T98G 细胞进行梯度剂量X 射线（0、2、4、6 Gy）照射。采用qPCR 检测miR-183、细胞质多腺 苷酸化元件结合蛋白1（cytoplasmic polyadenylation element-binding protein 1，CPEB1）在脑胶质瘤组织、T98G 细胞和经X 射线 照射的T98G 细胞中的表达量。将miR-183 inhibitor 转染T98G 细胞后下调miR-183 表达，经6 Gy X 射线垂直照射，CCK-8 法、 流式细胞术和WB 法，分别检测T98G 细胞增殖能力、细胞凋亡率及BAX 和Bcl2 蛋白表达量。Targetscan 软件预测和双荧光 素酶报告基因实验检测miR-183 与CPEB1 的靶向关系。下调CPEB1 表达后，经6 Gy X 射线照射，分别用CCK-8 法、流式细胞 术和WB 法检测T98G 细胞增殖能力、细胞凋亡率及BAX 和Bcl2 蛋白表达量。将pcDNA-CPEB1 或CPEB1 siRNA 质粒转染 T98G细胞，分别下调或过表达CPEB1 后，检测miR-183 通过CPEB1 对T98G细胞放射敏感性的影响。结果：脑胶质瘤组织和 细胞中miR-183 呈高表达，CPEB1 mRNA 呈低表达。T98G 细胞中miR-183 的表达量随着X 射线放射剂量增加而降低 （P<0.05），CPEB1 表达量随着X 射线放射剂量增加而升高（P<0.05）。6 Gy X 射线照射T98G 细胞后，下调miR-183 可降低细 胞增殖能力、增加细胞凋亡率，而过表达miR-183 则起到相反作用（P<0.05）。miR-183 靶向CPEB1 mRNA 且负调控CPEB1 表 达。下调CPEB1 表达后，经6 Gy X 射线照射可显著提高T98G 细胞增殖能力（P<0.05）、降低细胞凋亡率（P<0.05），miR-183 可 逆转CPEB1 过表达对细胞T98G 放射敏感性的促进作用（P<0.05）。结论：下调miR-183 的表达能够负调控CPEB1，从而增强 脑胶质瘤细胞的放射敏感性。