Aim To investigate the Clotrimazoles (CLT) inhibitive effect on the secretion of ?-defensins of the Paneth cells.Methods The ?-defensin mRNA expression is determined by semi-quantitative RT-PCR. The secretion of defensins is determined by dot-blot and ELISA. Results CLT has no influence on the defensin mRNA expression. All of 125 nmol?L -1, 250 nmol?L -1 and 500 nmol?L -1 CLT can inhibit the secretion of ?-defensins. Conclusion CLT could inhibit the secretion of defensins, which suggests that CLT maybe influence the function of small intestine

Objective To investigate the effects of radix salvia miltiorrhizae on the apoptosis of hepatoeytes during cold preservation and reperfusion injury in rat donor liver. Methods Forty male SD rats were divided into experimental group, control group and sham operation group. The rat model of liver transplantation was established according to the Kamada method. The grafts were preserved in lactated Ringer's solution with radix salvia miltiorrhizae in experimental group and in lactated Ringer's solution in control group for 5 hours, then they were transplanted orthotopically. Six hours after transplantation, the recipients were sacrificed, and the serum ALT and AST were detected by automatic biochemistry analyzer, the hepatocyte apoptosis by TUNEL, the expression of Bcl-2 and FasL protein by flow cytometry. The histopathological changes of the liver grafts were observed under light microscope. Results The levels of ALT and AST in the experimental group were significantly lower than those in the control group after reperfusion. Compared with that in the control group, the apoptosis index of the hepatoeyte was signifieandy decreased in the experimental group ( F = 133.802, P <0.05 ), while the level of Bel-2 protein expression was increased ( F = 91.063, P < 0.01 ). No statistical difference upon FasL protein expression was detected between the 2 groups( F = 1.329, P >0.05). The histopathological injury of the liver grafts in the experimental group was significantly slighter than that in the control group. Conclusions Radix salvia mihiorrhizae inhibits the apoptosis of hepatocytes by increasing the Bcl-2 protein expression during cold preservation and reperfusion injury, so it has a protective effect on the liver graft against ischemia reperfusion injury.

Mesenehymal stem cells CMSCS are nonhematopoietie stromal cells that are capable of differentiating into, and contribute to the regeneration of, mesenchymal tissues such as bone, cartilage, muscle, ligament, tendon and adipose. MSCs have been isolated from human first- and second-trimester fetal blood, liver, spleen, bone marrow, adipose tissue, amniotie fluid, periosteum and embryo. Not only can they express many phynotypes involving adhesion molecules and chemokine receptors, but also have character of immune tolerance, so they have potential of migration and transportation to target issue and feature of immune suppressing. There are wide application prospects for a number of areas in the cardiovascular, neurological, blood, surgical injury and other diseases. The current review describes the phynotypes, the immune tolerance and the potential for homing of MSCs.

Generally speaking,the hepatocyte stem cell is not a specific cell type.but an overall name of all kinds of cells that possess stem cell characters about embryonic development and regeneration of liver.HSC is a pluripotential stem cells which have self-renewal capacity and could differentiate into mature hepatocytes and bile duct cells.According to the different origin of hepatocyte stem cell,it can be usually divided into two kinds:non liver-derived hepatocyte stem cell and liver-derived hepatocyte stem cell.

Objective To investigate the therapeutic effect and safety of micro-dosage heparin therapy for acute leukemia with pre-diffuse intravascular coagulation (pre-DIC).Methods 36 cases of acute leukemia with pre-DIC were divided into two groups,18 cases were treated with micro-dosage heparin therapy (group A),18 cases were treated with conventional therapy(group B).Results Within the first 10 days,the cases which transformed to diffuse intravascular coagulation (DIC) in group A were significantly lower than group B(P

In the era of precision medicine,liquid chromatography-mass spectrometry(LC-MS)will continue to have significant impact on laboratory medicine in spite of the inherent challenges posed by the technology.It seeks to explain what is clinical mass spectrometry,and describes its basic framework and life cycle.It concludeds the key points of clinical LC-MS through the practical experiences of CLSI guideline and Cleveland health care clinical mass spectrometry laboratory.It then illustrates the challenges of appropriately applying LC-MS to clinical diagnostics through comparisons with research based LC-MS utilizations. Furthermore,It attempts to elucidate the challenges and endeavors It has made toward implementing clinical LC-MS in China.Finally,It discusses the prospective future of LC-MS in clinical diagnostics.

AIM To discuss the effects of losartan on AT 1R mRNA expression and renin angio tensin system (RAS) level in brain of spontaneously hypertensive rat (SHR). METHODS Sixteen 6 week old male SHR and 16 sex and age matched WKY were divided into losartan ( n =8) and control groups ( n =8) respectively. Losartan (30 mg?kg -1 ?d -1 ) or equal volume of 0.9% saline solution was administered for 18 weeks by gavage. AT 1R mRNA expression was examined by reverse transcription polymerase chain reaction (RT PCR). Renin activity (RA) and angiotensinⅡ(AngⅡ) levels were measured by radio immunoassay. Angiotensin converting enzyme activity (ACEA) was measured by ultraviolet spectrophotometar. RESULTS Enhanced brain AT 1R mRNA expression was found in SHR compared with WKY ( P 0.05, respectively). CONCLUSION The increase of AT 1R mRNA expression and RAS levels in brain may be involved in the pathogenesis of hypertension in SHR. Losartan protects brain by decreasing expression of brain AT 1R mRNA.

Objective To explore the effects of music intervention on growth and development of premature infants. Methods 112 premature infants were randomly divided into the observation group and the control group with 56 cases in each. The control group received conventional nursing measures, and the observation group was simultaneously given music intervention besides the conventional routine nursing measures. Weight increase, hospitalization time, milk-intake volume and feeding tolerance were compared between the two groups. Results The weight increase in the observation group was higher than that in the control group. Hospitalization time and rate of feeding intolerance for the observation group was lower compared with that of the control group. The premature infants in the observation group took in more milk than those in the control group. Conclusions Music intervention can elevate feeding tolerance, facilitate nutrition and increase weight of premature infant, so it is beneficial to the growth and development of premature infant.

Objective To summarize the experience of HIV/AIDS preventive interventions among out-of-school teenagers and provide scientific evidence for the development of large-scale interventions in the future. Method Using both non-random snowball and cluster sampling approach,a total of 5,000 participants were selected from 7 provinces (9 provincial sites ) and a cross-sectional survey was conducted by employing a semi-open-ended self-administrative interview schedule to evaluate their behavioral change before and after the interventions. Results The rate of HIV related knowledge and condom use increased significantly among the participants. For instance,the rate of condom use when having sex at the last time increased by 34.86%,i.e. from 27.67% at the baseline to 59.88% after the intervention (?2=34,86,P

Background Oxidative stress has been implicated in the pathogenesis of angiotensin Ⅱ(Ang Ⅱ)related hypertension.Superoxide anion,produced by NAD(P)H oxidase,plays important roles in hypertension and its complications.Ang Ⅱ infusion increases cerebral NAD(P)H oxidase activity,which is inhibited by angiotensin Ⅱ receptor blocker(ARB)candesartan and NAD(P)H oxidase inhibitor.However,the underlying mechanism of ARB in preventing hypertensive stroke is not elucidated clearly.Objective To investigate the effects of candesartan on the expression of cerebral NAD(P)H oxidase mRNA in salt-loaded stroke-prone spontaneously hypertensive rats(SHRsp).Methods Twelve-week-old salt-loaded SHRsp were treated with candesartan [1.0 mg/(kg?d)],trichlormethiazide [TCM,1.6 mg/(kg?d)] or vehicle(n=12 in each)for 2 weeks.Age-matched salt-loaded WKY rats were served as control(n=12).Blood pressure was measured every week.After two weeks,cerebrums were harvested and 24 h urine was collected.Urinary albumin was examined by ELISA.Cerebral cortex NAD(P)H oxidase subunits(p22phox,p47phox and gp91phox)mRNA expression were assayed by real-time PCR.Results The systolic blood pressure was increased significantly in salt-load SHRsp.Candesartan and TCM reduced SBP to the similar level.Urinary albumin excretion and cerebral cortex NAD(P)H oxidase subunits were markedly higher in salt-loaded SHRsp than those in salt-loaded WKY rats.Incidence of stroke was significantly reduced in candesartan treated group as compared with TCM treated SHRsp(P