1.Effect of monoclonal antibody to CD47 molecule on dendritic cell differentiation and function
Jing YU ; Maofang LIN ; Haibo MOU ; He HUANG ; Zhen CAI
Chinese Journal of Pathophysiology 2000;0(12):-
AIM: To explore the influence of CD47 molecules on the maturation and function of cultured dendritic cells (DCs). METHODS: Monocyte cell-derived DCs were propagated in granulocyte-macrophage colony-stimulating factor (GM-CSF) and lipopolysaccharide (LPS) plus interleukin (IL)-4, in the presence or absence of anti CD47 monoclonal antibodies (anti-CD47 mAbs). Flow cytometry was used to detect the cell surface phenotype. The concentration of IL-12P70 in supernatant was measured by ELISA technique. The antigen-presenting functions of DCs were determined in one-way mixed leukocyte reaction by Brdu-ELISA. Electrophoretic mobility shift assays (EMSA) was used to examine NF-?B activity. RESULTS: The anti-CD47 mAbs markedly suppressed the expression of CD80,CD86,CD83,CD1a,HLA-DR on the surface of DCs (P
3.ANOM in the optimization for promotion appraisal of hospital head nurses
Jingjing XIONG ; Zhen HE ; Liangxing SHI ; Juntao FANG ; Tianzhi YU
Chinese Journal of Hospital Administration 2013;29(4):300-305
Promotion of outstanding people to head nurses is key to hospital nursing management.In view of the current shortcomings in the analysis of appraisal data and the interpretation of appraisal results,the analysis of means(ANOM)method is introduced to the process of hospital human resource management.The proposed method aims to solve the problem when the score differences between interviewers are great and the score differences between candidates are small.An example for head nurses' promotion appraisal in a hospital is offered to demonstrate that statistical tools can provide the decision support for hospital personnel selection.Finally,the application differences between ANOM and analysis of variance(ANOVA) in human resources appraisal are compared.
4.A measurement of serum ferritin of acute lung injury patient
Bi-Fen CHEN ; Zhen-Yu QIN ; Jing HE ;
Chinese Journal of Primary Medicine and Pharmacy 2006;0(10):-
Objective To explore the relationship between the change of serum ferritin level and acute lung injury.Methods 192 cases living hospital patients with critical factor of acute lung injury were divided into two groups:acute lung injury group(study group,52 cases)and having critical factor of acute lung injury but not devel- oped acute lung injury group(control group,140 cases).50 people at the same time were selected as normal group. Serum ferritin level were measured,and their correlation between groups were analyzed.Results The serum ferritin level in study group[female(354.7?9.8)/,g/L,male(789.7?30.8)tag/L]increased significantly as compared wit h that in control group[female(182.7?7.8)/,g/L,male(312.5?1.9)?g/L](P
5. Correlation analysis of gout with body mass index and waist-hip ratio
Yu-ming CHEN ; Zhen-zhen LI ; Lu LIU ; Shuang HE ; Tuersun Xiayidai ; Lei MIAO
China Tropical Medicine 2022;22(12):1174-
Abstract: Objective To explore the relationship between body mass index (BMI), waist-hip ratio (WHR) and the risk of gout in Urumqi. Methods A total of 516 male patients with gout in a third-class hospital in Urumqi from 2015 to 2019 were randomly selected as the gout group and 516 male healthy subjects in the same hospital as the control group. The relevant blood biochemical indexes were examined and analyzed. Blood pressure, waist circumference and hip circumference were measured. Body mass index and waist-to-hip ratio were calculated. Logistic regression model was used to analyze the relationship between overweight / obesity, waist-to-hip ratio and the risk of gout. The test level is α = 0.05. Results Uric acid, glucose, urea nitrogen, creatinine, triglyceride, low-density lipoprotein, systolic blood pressure, weight and waist circumference in gout group were higher than those in control group, and the differences were statistically significant (P<0.05); There were no significant differences in age, height and diastolic blood pressure between the two groups (P<0.05). There was a positive correlation between BMI and WHR and the occurrence of gout (r=0.272, 0.345, P<0.05). There were significant differences in BMI, WHR and waist circumference between the gout group and the control group(χ2= 55.338, 54.928, 54.153, P<0.05). After adjusting for age, aerobic exercise and other confounding factors, the results of multi-factor unconditional Logistic regression analysis showed that the odds ratio (OR) of gout in patients with BMI of 24.00-27.99 kg/m2 and ≥28.00 kg/m2 was 2.005 (1.337-3.006) and 2.677 (1.668-4.296) times higher than that of patients with normal BMI, respectively. The OR value of gout in patients with WHR≥0.90 was 1.668 times higher than that in patients with normal WHR, and the difference was statistically significant. The results of subgroup analysis according to age are generally similar. Conclusions The BMI and WHR of man with gout in Urumqi are higher than those of normal people, and BMI, waist circumference and WHR are all associated with the incidence of gout. The risk of gout increases with the increase of BMI and WHR.
6.Advancements in stabilization technologies for membrane protein and its application in drug screening
Jia-hao FANG ; Yu-hong CAO ; Yu-zhen HE ; Zhan-ying HONG ; Yi-feng CHAI
Acta Pharmaceutica Sinica 2021;56(9):2325-2334
Membrane proteins are the main undertakers of biofilm function, and also the most important target group for innovative drug discovery and research. About 60% of drugs targets are membrane proteins. Due to the obvious aggregation and denaturation tendency of membrane proteins in aqueous solution, it is difficult to simulate the membrane like environment to maintain the correct conformation of membrane proteins
7.Protein interaction network analysis of Panax notoginseng saponins.
Zhen-Zhen REN ; Yan-Ling ZHANG ; Xing WANG ; Shi-Feng WANG ; Yu-Su HE ; Chen-Xi ZHAI ; Yan-Jiang QIAO
China Journal of Chinese Materia Medica 2014;39(11):2097-2101
Panax notoginseng (PN) is one of the commonly used clinical medicines for cardiovascular diseases and possesses a variety of pharmacological effects. P. notoginseng saponins (PNS) are the most important bioactive components in PN. The purpose of this study was to explain the mechanism of PNS on molecular network level. 18 targets of the main medicinal ingredients of PNS were gained by virtual screening based on pharmacophores and data mining. A protein interaction network of PNS was constructed with 189 nodes and 721 interactions. By a graph theoretic clustering algorithm Molecular Complex Detection (MCODE), 14 modules were detected. Gene ontology (GO) enrichment analysis of the modules demonstrated that the roles of PNS played in cardiovascular disease related to multiple biological processes, which could represent the characteristics of traditional Chinese medicine (TCM) as a whole to regulate the disease. The results showed that the blood circulation and hemostasis efficacy of PN related with the biological processes such as positive regulation of cAMP metabolic and biosynthetic process, platelet activation and regulation of blood vessel size, regulation of T cell proliferation and differentiation and so on. Therefore, the module-based network analysis will be an effective method for better understanding TCM.
Drugs, Chinese Herbal
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chemistry
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Humans
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Panax notoginseng
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chemistry
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Protein Interaction Maps
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drug effects
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Proteins
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chemistry
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Saponins
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chemistry
8.Analysis of 105 cases of pregnancy -induced hypertension patients with Ⅲphase of retinopathy
Xiao-Yun, QIU ; Jian-Ling, GUO ; Feng-Ci, YU ; Shu-Zhen, LIANG ; Zhen-An, LI ; Qing-Quan, HE
International Eye Science 2014;(9):1699-1701
To explore the related risk factors and outcome in pregnancy- induced hypertension patients with Ⅲ phase of retinopathy.
●METHODS: A total of 105 pregnancy - induced hypertension patients with Ⅲ phase of retinopathy in our hospital from Januany 2012 to December 2013 were enrolled. Clinical date of them were collected to analyze.
●RESULTS: The occurrence of pregnancy - induced hypertension patients with Ⅲ phase of retinopathy were positively correlated with the course of the disease, blood pressure, proteinuria, and it was higher occurred in cold winter and spring, timely termination of pregnancy and appropriate hormone therapy can promote the recovery of vision, and improve outcomes of pregnancy.
●CONCLUSlON: The occurrence of pregnancy - induced hypertension patients with Ⅲ phase of retinopathy associated with season and disease severity. Timely treatment can restore normal vision, improve maternal and neonatal prognosis. Routine examination of fundus examination should be used as the pregnancy induced hypertension syndrome.
9.The Top-quality Course Construction in Forensic Pathology and Its Relevant Problems
Zhen LI ; Lihua LI ; Yongqiang QU ; Jianyun YU ; Puping LEI ; Yonghe ZHAO ; Yingzao HE
Journal of Kunming Medical University 2007;0(S2):-
The top-quality course construction,in comprehensive medical university,is very important for the undergraduate teaching evaluation. The paper discussed the top-quality course construction in forensic pathology and the relevant issues in medical college course teaching,such as the status of the course,characteristic in school-running and enforcement measures,which aimed to improve cognition and enhance teaching quality and teaching level.
10.Expressions of Let-7 in Normal Breast Tissue and Breast Cancer Tissue
Zhen YU ; Mingyao XIAO ; Ming CUI ; Min LIU ; Shuying DAI ; Ying CHEN ; Yuefeng HE
Journal of Kunming Medical University 2016;37(7):19-21
Objective To study the expression characteristics of Let-7 genes in breast cancer.Methods Twenty-eight patients with breast cancer were randomly selected,and their cancer tissue and adjacent normal tissue were collected.TRIzol was used to extract the total RNA and real-time quantitative PCR was used to detect the relative gene expression levels.Results The expression of precursor Let-7 in cancer tissue was (9.65 ± 2.31),which was lower than that in normal tissue (10.05 ± 2.81),P =0.048.Precursor Let-7 had dependence relationship with the long menstruation (b =0.816,P =0.029).The menarche age showed a positive correlation with precursor Let-7 in normal tissue (r =0.502,P =0.048) and a negative correlation in cancer tissue (r =-0.484,P =0.049) Conclusions The expression of precursor Let-7 in cancer tissue is lower than that in normal tissue.The period of menstruation is a protective factor to breast cancer.