Objective To observe the clinical efficacy of triple needling and centro-square needling in treating hemifacial spasm. Method Sixty-eight patients with facial spasm were randomized into a treatment group and a control group, 34 in each group. The treatment group was intervened by centro-square needling at topical Ashi points and triple needling at Yifeng (TE 17) on the affected side; the control group was intervened by regular acupuncture. The two groups were treated once a day, 10 times as a treatment course, for 3 courses in total. Cohen Albert grading scale was adopted to evaluate the spasm intensity before and after the intervention, and the clinical efficacies were then compared.Result After intervention, there was a significant difference in comparing the spasm intensity between the two groups (P<0.05). The significant improvement rate was 88.2% in the treatment group versus 50.0% in the control group, and the difference was statistically significant (P<0.05).Conclusion Triple needling plus centro-square needling is an effective method in treating hemifacial spasm. This method uses less points, but it produces a high efficacy, strong pertinence, and is easy-to-operate.

Objective To analyze the factors influencing augmentation index (AI) by examining the AI changes in patients who received continuous ambulatory peritoneal dialysis (CAPD) therapy, and evaluate the artery elastic function situation. Methods The height, weight, blood pressure, blood indicators were detected in 46 CAPD patients (CAPD group) and 42 controls (control group). AI was managed by OMRON HEM-90OOAI, and the influencing factor was analyzed. Results Systolic blood pressure(SBP),diastolic blood pressure(DBP), pulse pressure, central aortic pressure and AIp75 (AI value, when pulse was 75 beats/min) in CAPD group were significantly higher than those in control group. Within CAPD patients,the AIp75, in female was significantly higher than that in nude [(96.67±8.95 )% vs (89.89±6.46)% ,P =0.022]. Simple correlation showed positive relationship in gender, age, SBP, DBP, pulse pressure and central aortic pressure with AI; and negative relationship in height, weight and weight index with AI; while there was no relationship between AI and all kinds of chemical examination indicators or dialysis years.Multiple linear regression analysis evidenced that gender,age, height, DBP and central aortic pressure of CAPD patients had linear regression relation with AI. Conclusions It indicates a decreased artery elasticity and an increased stiffness occurs in CAPD patients. AI has tight correlation with age, height, DBP and central aortic pressure, and is an non-invasive factor thai partially reflects the arterial elasticity. Central aortic pressure is relevant with aortic elasticity and rigidity closely. Measuring central aortic pressure is very imporrant for preventing cardiovascular accident.

BACKGROUND:Articular injections of opioids are widely adopted for pain management of total knee arthroplasty, with both peri-articular and intra-articular administration routes. Recent studies have indicated that commonly used anesthetics, steroids and non-steroidal anti-inflammatory drugs are associated with potential adverse effects. Peripheral opioids are relatively safe with lower complication rates, but great controversy exits regarding the analgesic effects. OBJECTIVE:To introduce the current clinical application status and basic research progress in peri-articular and intra-articular opioid injections for postoperative analgesia of knee arthroplasties. METHODS:A computer-based search in PubMed and Embase databases was conducted for clinical and basic research articles related to pain management of knee arthroplasties using peri-articular and intra-articular injections of opioids published from January 1967 to May 2015, using the keywords of“opioids;peri-articular injection;intra-articular injection;analgesia;knee arthroplasty”in English. Irrelevant, poorly related and repetitive studies were excluded. RESULTS AND CONCLUSION:Peri-articular and intra-articular opioids injection plans vary greatly between different institutions, which does not support reliable quantitative data synthesis for a meta-analysis. Controversy exists regarding its efficacy, but there are studies supporting the usage of opioids for providing effective analgesia in a dose-dependent manner. The peri-articular administration route is associated with lower complication rates including nausea and vomiting compared to systematic injections. Applications of opioids help to avoid potential chondrocyte and stem cel cytotoxicity caused by other anesthetics, steroids and non-steroidal anti-inflammatory drugs, which is important for pain management of total knee arthroplasties. Future investigations are required to promote articular analgesic effects and time duration by exploring more advanced drug combinations and dosage forms.

ObjectiveTo screen for proteins related to invasive and metastatic potential of ovarian cancer.MethodsThe nude mice were intraperitoneally injected with the human ovarian cancer 3AO cell line.The metastatic tumors were isolated and cultured in vitro.The cell lines were established from tumors and transplanted intraperitoneally for several passages.Invasive potential of cell lines was evaluated by cell adhesion and matrigel invasion assays.Lysates from different cell lines with high or low invasive potential were analyzed by surface enhanced laser desorption/ionization-time of flight-mass spectrometry(SELDI-TOF-MS)and protein-chip array technology.The proteomic fingerprint patterns were compared. ResultsThe subclones 3AOF3 and 3AOF4 were isolated from repeated passaging in vivo and vitro,and showed higher invasive capacity than 3AO cells.By comparing their proteomic fingerprints,9 distinct proteins (3494,3831,8121,8293,9123,9332,10 184,15 436 and 17 134 at m/z value) were found,which were expressed only in 3AO cells.On the contrary,1 distinct protein (4360 at m/z value) was expressed only in 3AO F3-4 cells,and 2 distinct proteins (1842 and 3145 at m/z value) were expressed only in 3AO F2-4 cells.Additionally,6 distinct proteins (1236,5476,5649,6575,6897 and 7016 at m/z value) and a mass cluster (13 794 and 14 027 at m/z value)were expressed in all 3AO and 3AOF1-4 cells,while the amount of protein was different.ConclusionBy comparing the proteomic fingerprints of isogenic ovarian cancer cells with different invasive potential,a group of proteins are identified which are correlated with high invasive and metastatic potential.

AIM: To study the curative effect for patients with age related cataract and shallow anterior chamber after phacoemulsification.METHODS: Totally 38 patients (38 eyes) with age related cataract and shallow anterior chamber were selected and divided into two groups according to the depth of the anterior chamber, as mild shallow anterior chamber group (2-2.5mm) 23 eyes, high risk shallow anterior chamber group (<2.0mm) 15 eyes.Thirty-eight patients (38 eyes) with age related cataract with normal anterior chamber were as control group at the same period.All the patients received the operations by the same doctor and were followed up for 3mo.The observed items included visual acuity before and after operations, intraocular pressure, anterior chamber depth, corneal endothelial cell density and complications.RESULTS: There were no significant difference on visual acuity, intraocular pressure and corneal endothelial cell density between the two groups before operations (P>0.05).The visual acuity improved significantly after operation in both groups (P<0.05).Intraocular pressure after operation decreased significantly in both groups (P<0.05).Anterior chamber depth increased significantly after operation in both groups (P<0.05).Corneal endothelial cell density decreased significantly in both groups (P<0.05).There were no significant difference on anterior chamber depth, intraocular pressure and corneal endothelial cell density between the two groups at different time point after operations (P>0.05).Posterior capsular rupture occurred in shallow anterior chamber group in 1 eye, suspensory ligament rupture in 1 eye.Posterior capsular rupture and suspensory ligament rupture occurred none in normal anterior chamber group.Postoperative corneal edema occurred in 10 eyes (26%) in shallow anterior chamber group, which occurred in 3 eyes (8%) in normal anterior chamber group.The difference on the incidence was significant (P <0.05).CONCLUSION: Phacoemulsification should be taken timely for patients with age related cataract and shallow anterior chamber.The postoperative visual acuity can be improved and the anterior chamber depth can increase.The operation is safe and effective for those patients.

OBJECTIVE: To establish an HPLC method for the determination of the relevant substances in tegafur injection.METHODS: The determination was performed with a Kromasil C18 column with the column temperature maintained at 30 ℃,and the mobile phase was methanol-water(25∶75) at a flow rate of 1.0 mL?min-1.The UV detection wavelength was set at 282 nm and the sample size was 20 ?L.RESULTS: The relevant substances were well-separated from the chief agent,and the contents of the relevant substances in the sample were 2.35%,2.37%,and 2.24%,respectively.CONCLUSION: Compared with TLC,HPLC is more accurate and sensitive,and it is applicable for the determination of the relevant substances in tegafur injection.

Objective To study the influence of RNA interference Caspase-3 gene on apoptosis of transformed human embryonic kidney 293 cells induced by cadmium in vitro. Methods Transformed human embryonic kidney 293 cell were treated with siRNA for 36 h. The treated cells were incubated with 40 ?mol/L CdCl2 for 12-24 h and the cells viability were measured with methyl thiazolyl tetrazolium(MTT) assay. In addition,the expression of caspase-3 gene in 293 cells was detected by the methods of reverse transcription polymerase chain reaction (RT-PCR) and Western-blot analysis,and the occurrence of apoptosis was determined by flowcytometry with Annexin-V and propidium iodide (PI) double labeling method. Results The results of RT-PCR and Western-blot analysis revealed that in incubated cells with 40 ?mol/L cadmium 12 h,the expression of Caspase-3 mRNA and 20 kDa protein significantly increased when compared with controls(n=4,P

[Objective]To study the clinical characteristics of osteochondrosis of lumbar posterior vertebral edge(OLPV) and evaluate the efficiency of surgical treatment. [Method]From August 2000 to August 2003,41 cases of OLPV patients were treated,all cases underwent decompression of posterior approach and selective resection of the compressive things.Furthermore,1 case was treated with trans-vertebral pedicle internal fixation and inter-transverse process bone graft because of excessive decompression and preoperative lumbar instability.[Result]Low back and leg pain disappeared or obviously being relieved in all cases,and the results of histological analysis suggested the diagnosis of OLPV in all cases.Forty-one cases were followed from 5 to 8 years,average 6.8 years.No lumbar instability or spondylolisthesis was found in any case.Six cases suffered slight low back pain occasionally without obvious impaired quality of life.The preoperative JOA score was 14.73?2.06,when followed by 3 months,the score changed into 27.14?4.13,there was statistical significance between them(P

[Objective]To analyze the dynamic changing of inflammatory markers in second stage revision for infected TKA and to explore the sensitive indicators. [Methods]From June 2004 to April 2008,20 cases of infected TKA were admitted and treated by the two stages knee revision.The inflammatory markers included full blood count,erythrocyte sedimentation rate(ESR).C-reactive protein(CRP) were examined weekly,and to evaluate the dynamic changes and the most sensitive indicators.[Results]CRP was the most sensitive index for infection control,and its maximum presented in the first week post antibiotic bone cement implantation,followed by rapid reduction in the second week and there was statistical difference compared to the CRP level before surgery(t=105.84,P