Objective:To study the effect of roadside dust inhalable particulate matter on airway inflammation in rats.Methods:Particles at roadside were collected,analyzed and PM10-treat model of rats were established.Lung tissue histopathology was observed.The counts of total leukocytes and neutrophils in bronchoalveolar lavage fluid(BALF)and superoxide dismutase(SOD),glutathion peroxidase(GSH-Px),malondiadehyde(MDA),cytokin-induced neutrophil chemotactics(CINC)in lung homogenate were measured.Results:The counts of total leukocytes,macrophages and neutrophils in PM10-treat group were increased significantly than those in control group(P

Objective:To investigate the role of nuclear factor-?B(NF-?B)in rats with cerebral ischemia-reperfusion injury. Methods:Rat models of cerebral ischemia-reperfusion were established.24hours later,the expression of NF-?B in rat brain of sham-operation group,operation group and intervention group was detected with immunohistochemistry method,Cytokin-induced neutrophil chemotactics(CINC)in rat brain were measured and histopathological examinations were conducted on the hippocampal CAl.Results:The expression of NF-?B p65 and CINC in the operation group increased significantly compared with the sham-operation group(P

OBJECTIVE:To explore the implementation methods of drug price reform in China. METHODS:Current condi-tion and disadvantages of drug price mechanism in China were analyzed,the problems of the implementation of drug price reform were concluded and summarized,and the implementation methods of drug price reform were put forward. RESULTS & CONCLU-SIONS:Current condition and disadvantages of drug price mechanism in China are mainly reflected as failure of policy for maxi-mum retail price causes part of drugs to be overpriced;government-led centralized procurement of drugs results in that part of drugs are underpriced;the defects in the regulation of drug price addition rate leads to hospitals’procurement of drugs at higher price;government’s excessive interference in drug price affects the fair competition and sound development of pharmaceutical in-dustry. However,there are some problems for drug price reform,including unsound drug distribution system,a lack of fair compe-tition in modern drug market,excessive centralization of power of medical insurance authorities,absent public participation and so-cial supervision,and the“practice of using pharmacy profits to subsidize medical services”leading to wrong medical behavior. These measures were suggested to resolve above problems,i.g. reform the drug distribution system,foster modern drug market,es-tablish a medical insurance authorities-led mechanism for drug price negotiation in which multiple interest parties participate,and develop drug retail enterprises gradually replacing the outpatient pharmacies in hospitals to separate medical services from pharma-ceutical services.

Objective: To investigate the effects of the tandem repeat polymorphisms in the enhancer region (ER) of thymidylate synthase (TS) gene and the 6-bp deletion/insertion (del6/ins6) polymorphism in the 3′ untranslated region (3′-UTR) of TS gene on the clinical outcomes of gastric cancer patients treated with 5-FU-based adjuvant chemotherapy. Methods: One hundred and sixteen patients with gastric cancer were treated with 5-FU-based adjuvant chemotherapy. The polymorphisms of TSER and TS 3′ -UTR in those patients were determined by polymerase chain reaction (PCR) method. Results: Of the 116 patients, the frequencies of the TSER 2R/2R, 2R/3R and 3R/3R were 7.8% (9/116), 31.9% (37/116), and 60.3% (70/116), respectively; the frequencies of the TS3′-UTR ins6/ins6, ins6/del6 and del6/del6 were 9.5% (11/116), 41.4% (48/116) and 49.1% (57/116), respectively. The median survival period in ins6/ins6 carriers was significantly shorter than that of del6/del6 (P = 0.017) or ins6/del6 (P = 0.022) carriers. There was no significant difference in median relapse-free survival period between different TSER carriers (P > 0.05). COX multivariate analysis showed that the ins6/ins6 carriers had increased death risk (P <0.05) compared to the other two genotypes. The median no-recurrence survival period had no statistical difference between them. Conclusion: The polymorphism of TS 3 UTR ins6/del6 may be an independent factor for the prognosis of gastric cancer patients treated with 5-FU-based adjuvant chemotherapy.

Emergency Treatment ; methods ; Humans ; Seizures ; therapy ; Status Epilepticus ; therapy

Angioplasty, Balloon, Coronary ; methods ; Coronary Artery Bypass ; methods ; Coronary Vessels ; surgery ; Humans

Antitussive Agents/*pharmacology ; Drug Evaluation, Preclinical/methods

Objective To analyze the clinicopathologic features and prognosis of early stage cervical adenosquamous carcinoma and adenocarcinoma.Methods Clinical data of 62 patients with cervical adenosquamous carcinoma,149 patients with adenocarcinoma and 2 687 patients with squamous carcinoma of stage Ⅰ B-Ⅱ A,who received surgical treatment and adjuvant radiotherapy from June 2006 to February 2012 were retrospectively analyzed.The Chi-square test,Kaplan-Meier method,log-rank test and logistic regression were used for statistical analysis.Results In patients with adenosquamous carcinoma,adenocarcinoma and squamous carcinoma,the rates of lymph node metastasis were 33.6％ (50/149),29.0％ (18/62) and 22.0％ (591/2 687),deep stromal invasion were 64.4％ (96/149),75.8％ (47/62) and 55.3％ (1 486/ 2 687) and corpus invasion were 26.2％ (39/149),25.8％ (16/62) and 6.7％ (181/2 687),respectively.Those pathological parameters in adenosquamous carcinoma and adenocarcinoma were higher than those in squamous carcinoma (χ2 =12.170,P=0.002;χ2 =14.660,P=0.001;χ2 =97.732,P=0.000).There were no difference between adenocarcinoma and adenosquamous carcinoma in proportion of massive tumor (≥4 cm) [38.9％ (58/149) vs.35.5 (22/62),χ2 =0.220,P =0.639],lymph node metastasis (χ2 =0.410,P =0.522),corpus invasion (χ2 =0.003,P =0.956),deep stromal invasion (χ2 =2.595,P =0.107) and low differentiation[38.9％ (58/149) vs.48.4％ (30/62),χ2 =1.612,P=0.204].The median follow-up period was 45 (3-92) months.Multivariate analysis showed that corpus invasion (P =0.014,RR =3.393,95％CI:1.280-8.991),age (P=0.000,RR =1.077,95％ CI:1.037-1.117) and stage (P =0.007,RR =0.275,95％ CI:0.108-0.699) were prognostic factors of adenosquamous carcinoma.Age (P =0.006,RR =1.025,95 ％ CI:1.007-1.043) and lymph node metastasis (P =0.000,RR =2.525,95％ CI:1.547-4.120) affected the prognosis of adenocarcinoma.The median survival times for adenocarcinoma,adenosquamous carcinoma and squamous carcinoma were 45,46 and 56 months (adenosquamous carcinoma vs.squamous carcinoma:χ2 =1.347,P =0.246;adenosquamous carcinoma vs.adenocarcinoma:χ2 =1.808,P=0.179).There were no difference in recurrence rates between adenosquamous carcinoma and squamous carcinoma[14.0％ (7/50) vs.7.6％ (175/2 298),χ2 =1.968,P=0.161],and between adenosquamous carcinoma and adenocarcinoma [14.0％ (7/50) vs.22.1％ (27/122),χ2 =1.478,P =0.224].Conclusions Clinicopathologic features are similar between stage Ⅰ B-Ⅱ A cervical adenosquamous carcinoma and adenocarcinoma.Corpus invasion,age and stage are the leading indicators affecting the prognosis of adenosquamous carcinoma.Age and lymph node metastasis are the factors affecting the prognosis of adenocarcinoma.There are no difference in survival and prognosis between adenosquamous carcinoma and adenocarcinoma.

Objective To evaluate early diagnositic value of thyroid transcription factor-1(TTF-1) for patients with lung cancer.Method Ninety-nine lung cancer tissue samples and 21 non-neoplastic lung tissue samples were collected from surgical resections.TTF-1 expressions in the above samples were assessed by micro array combined with immunohisto-chemistry assay.According to percentage of reactive cells,tissue immunoreactivity for TTF-1 were categorized into 3 groups :negative(

Objective To ascertain and evaluate the quality of radiological protection in rural and urban primary health care institutions . Methods Questionnaires were designed to investigate the protection levels in diagnostic radiology in primary health care institutions in six cities in 2013 and to evaluate the results by using the technique for order preference by similarity to an ideal solution ( TOPSIS) combined with the rank sum ratio ( RSR) method.Results The six cities and an integrated case ( all the data of the six prefecture-level citys were merged as one for comprasion ) were ranked based on the comprehensive assessment.The order from good to bad was Suzhou , Dalian, Yichang, integrated case, Shaoxing, Quzhou and Heihe.The results were divided into three grades , with Suzhou, Dalian at best grade, Yichang, integrated case, Shaoxing, Quzhou at middle grade, and Heihe at poor grade. Conclusions The quality of protection in radiation diagnosis varied considerably .Suzhou ranked the first and Heihe ranked the last .The study suggests that efforts should be focused on the management of key cities and indicators to upgrade whole protection level in radiation diagnosis .