Objective To explore the application effect and safety of ultrasonic location in interscalene bra-chial plexus block anesthesia.Methods 100 cases with upper extremity orthopedic surgery patients were selected, according to the brachial plexus between the different positioning methods,they were divided into control group and observation group,50 cases in each group,the control group chose the traditional anatomical localization techniques, observation group interscalene under ultrasound guidance brachial plexus block,two groups of anesthesia were com-pared,block success rates and complications were observed.Results The block operation time,anesthesia onset time,duration of analgesia and anesthesia drug dosage of the observation group were (185.5 ±24.86)s,(11.55 ± 2.89)min,(11.42 ±2.39)min,(17.25 ±2.54)mL while the control group were (228.75 ±26.20)s,(16.05 ±4.66)min,(10.95 ±2.83)min,(19.50 ±2.79)mL,there was significant difference between two groups(t =18.34, 10.28,9.72,10.68,all P <0.05).the anesthesia success rate of the observation group was 98%,significantly higher than 88% of the control group,the difference between the two groups was statistically significant(χ2 =9.12,P <0.05).The incidence rate of complications in the observation group was 0,while the control group was 8%,two groups had significant difference in complication rate(χ2 =8.34,P <0.05).Conclusion Ultrasonic location in in-terscalene brachial plexus block anesthesia has a good clinical effect,with high safety,which is worthy of populariza-tion and application.

Objective To investigate the clinical value of blood recovery in the heart valve replacement surgery of adults. Methods Selected 556 patients accepted cardiac valve replacement surgery from May 2008 to April 2012 in our hospital,and divid-ed into control group(278 cases)and observation group(278 cases)according to the way of intraoperative blood transfusion patients in observation group underwent autologous blood recovery and patients in control group underwent without autologous blood recov-ery,the clinical parameters were observed and statistically analyzed.Results The differences of blood routine after 24 h(such as he-moglobin,hematocrit,platelets,etc.)and blood gas analysis(such as pH,potassium ions,lactic acid,etc.)between two groups were not significantly different,which is statistically insignificant(P >0.05);the postoperative chest drainage of patients in observation group was not significantly different from that of patients in control group,which was statistically insignificant(P >0.05);the a-mount of banked blood in observation group(263.83 ± 19.23)mL was significantly lower than that in control group(615.24 ± 20.34)mL,the difference between two groups was statistically significant(P <0.05).Conclusion In heart valve replacement sur-gery of adults,blood recovery can effectively add blood volume,reduce autologous blood loss,and significantly reduce the amount of banked blood input,which is worthy of promotion.

Standardized training of residents in the department of orthodontics is an important learn-ing stage for stomatology students before carrying out clinical orthodontic diagnosis and treatment work. At present, there are some deficiencies in the standardized training system of the residents in the Department of Orthodontics, such as the single teaching mode, imperfect training items, and unclear evaluation criteria. The department of orthodontics of Stomatology Hospital of Southern Medical University carried on a prelim-inary study on the training pattern of resident doctors in the refinement of the training content, optimizing curriculum design, implementation of tutorial teaching mode, the combination of theoretical study and clini-cal training, regular training evaluation and quantitative assessment indicators. The results showed that the trainees' acceptance and satisfaction were higher, which was beneficial to the improvement of clinical com-prehensive ability of orthodontics.

Objective To investigate effects of multimodal analgesia on cytokines and cortisol levels in patients received surgical treatment for radical colon cancer.Methods Eighty-six patients with radical colon cancer treated with surgery were randomly divided into control group and observation group.The control group only received routine epidural anesthesia and the observation group received multimodal analgesia.Preoperative and postoperative VAS scores,cytokines and cortisol levels were measured.Results Compared with those of the control group,the following 5 indicators decreased significantly in the observation group including VAS scores of after 24 h,48 h,72 h,IL-8 and TNF-α after 24 h,IL-6,IL-8 and TNF-α after 48 h,Cor after 24h,and Cor and ACTH after 48 h (P＜0.05).Conclusion Perioperative multi-mode analgesia helps relieve pain,stress and inflammatory cytokines among patients after the surgery for radical colon cancer.

OBJECTIVE:To investigate medical staff’s awareness and knowledge level on the rational use and management of antibacterials in our hospital.METHODS:A questionnaire survey was conducted on the spot among medical staff in our hospital,and the results of survey were recorded in Excel electronic form and subjected to data analysis and processing.RESU-LTS & CONCLUSION:The medical staff had a good mastering about the general knowledge about antibacterials(such as definitions,classifications and so on).However,their answers to the questions such as reasonable antibacterial drug use,bacterial resistance,safety and expertise were relatively concentrated(tended to tick the same answer).Therefore,it is advisable to develop different forms of training through different ways among hospital medical staff to enhance their knowledge level on antibacterials and strengthen their awareness of correct and rational use of antibacterials meanwhile setting related measures and management norms.

Adult ; Age of Onset ; Amino Acid Metabolism, Inborn Errors ; complications ; diagnosis ; genetics ; therapy ; Betaine ; administration & dosage ; therapeutic use ; Carrier Proteins ; genetics ; metabolism ; Child ; China ; epidemiology ; DNA Mutational Analysis ; Gas Chromatography-Mass Spectrometry ; Genotype ; Homocysteine ; urine ; Humans ; Hydroxocobalamin ; administration & dosage ; therapeutic use ; Hyperhomocysteinemia ; complications ; diagnosis ; genetics ; therapy ; Infant ; Methylmalonic Acid ; blood ; urine ; Mutation ; Vitamin B 12 ; metabolism

Objective To explore a kind of biomedical signal pattern (BSP) with a new method called as state representation methodology (SRM ) .Methods Based on the heart sound signals ,ECG signals ,breathing ,as the important research problem for BSP description ,with some comparisons on several traditional methods ,in which support vector machines (SVM ) and response sur-face methodology (RSM ) etc .,using frequency slice wavelet transform (FSWT ) method to extract the BSP signal dynamic damping characteristics ,thus ,this paper proposes a new idea of SRM analysis .In the case of heart sound signal analysis ,the general steps of SRM evaluation method is given .Results In 40 cases of normal heart sounds SRM model is set up ,with 80 cases of abnormal heart sounds are compared ,the obvious differences of the SRM state distributions of the two groups are found .Conclusion The combi-nation of SRM with FSWT can provide a novel approach for BSP analysis ,and provide powerful development tool for the analysis of BSP .

The precise measurement in lens thickness in vivo, provides great application value for intraocular accommodation and ametropia development mechanism research.And it has great clinical significance for the diagnosis and treatment of glaucoma and cataract.Currently, many ultrasonic methods and optical methods are used in measuring lens thickness.The measurement principles, advantages, disadvantages and the accuracy of the instruments are summarized in this paper.Among these methods, Orbscan II, Pentacam, Lenstar and AS-OCT can be used to measure lens thickness instead of A-scan.More important is the fact that UL-OCT can dynamically monitor the change of the lens thickness with intraocular accommodation.Choosing an instrument with higher measuring accuracy to examine the lens thickness, can provide more accurate and convincing lens thickness data for clinical and scientific research.

Objective Comprehensive geriatric assessment is the core technology,this paper describes the concept of comprehensive geriatric assessment,objects,content,assessment methods,interventions and research pro-gress,provide experience to carry out comprehensive geriatric assessment in Tibet,the Tibet region to explore for com-prehensive geriatric assessment mode.

Objective To investigate the effect of autophagy on the proliferation and migration of cervi-cal cancer cells ,as well as the underlining mechanisms .Methods Rapamycin was used to induce the autophagy in HeLa cells,formation of autophagosomes was observed by staining with acridine orange under fluorescence mi -croscope.Western blot was used to detect the expression of LC 3 and PI3K/Akt/mTOR in HeLa cells.The LC3 plasmid was transfected into HeLa cells .The distribution of LC3 in cells and the expression of LC3 was identified by fluorescence microscope and Western blot ,respectively.The autophagy was inhibited with 3-methyladenine(3-MA )in HeLa cells.The cell proliferation was monitored by RTCA real -time instrument.Transwell chamber was carried out to assess cell migration .Results After 6h of rapamycin treatment ,the expression of LC3B was in-creased in HeLa cells ( P<0 .05 ) .The proliferative and migration ability were weakened compared to wild type HeLa cells(P<0.05).The same results in the presence of 3-MA.The expression of PI3K/AKT/mTOR path-way proteins were activated by rapamycin treatment and LC 3 overexpression(P<0.05).Conclusion Autophagy can suppress the proliferation and migration in cervical cancer cells ,which may relate to PI3K/Akt/mTOR path-way.