1.Effects of interleukin-1βon MMP-13 expression in rat chondrocytes and its regulation of miR-27b
Tianjin Medical Journal 2015;(8):871-875
Objective To observe the effect of interleukin-1β(IL-1β) on expression of Matrix Metalloproteinases 13 (MMP-13) in rat chondrocytes and its regulation of miR-27b. Methods Chondrocytes were extracted from 7 Wistar male rats. Expression of MMP-13 were examined by Western blot at 0 h, 24 h, 48 h after IL-1βstimulation. Differential miRNAs expression profiles were examined by miRNAs microarray. The most obviously down-regulated miRNAs were confirmed by quantitative Real-time PCR. Targeted regulation relationship between miR-27b and MMP-13 was set up by Luciferase re?porter gene experiments. Results Expression of MMP-13 in rat chondrocytes was increased at a timely dependent manner upon IL-1βstimulation(P<0.05);Microarray revealed 36 miRNAs whose expression changed, among which 6(miR-27b, miR-31, miR-26a, miR-26b, miR-23, miR-204)were especially obvious. Real-time PCR confirmed that miR-27b was the one whose expression level were most down-regulated. Transient co-transfection of miR-27b mimics with luciferase expres?sion plasmids resulted in significant repression of luciferase activity in rat chondrocytes (P < 0.05). Conclusion IL-1βstimulation result in down-regulation of miR-27b and up-regulation of MMP-13 expression. MiR-27b and MMP-13 show targeted regulation relationship.
2.Analysis on clinical response of IA and DA regimens in the treatment of 74 newly diagnosed acute myeloid leukemia patients.
Xin-yu LI ; Xin WANG ; Ying LI
Chinese Journal of Hematology 2013;34(1):67-68
Adolescent
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Adult
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Aged
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Antineoplastic Combined Chemotherapy Protocols
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therapeutic use
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Cytarabine
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administration & dosage
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Daunorubicin
;
administration & dosage
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Female
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Humans
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Idarubicin
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administration & dosage
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Leukemia, Myeloid, Acute
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drug therapy
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Male
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Middle Aged
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Treatment Outcome
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Young Adult
4.Comparison of two extraction techniques for larger chemically aceilular nerve allografts
Xin WANG ; Yu WANG ; Jiang PENG
Orthopedic Journal of China 2006;0(09):-
[Objective]To compare two preparing procedures for larger chemically acellular nerve allografts (CANA). [Methods]The sciatic nerves of pigs were exposed by a muscle-splitting incision and were isolated free of the underlying fascia. The 60-mm-long segments of the nerve were obtained. They were treated according to the following decellularization processes.In group I,the nerve segments were treated with 7% Triton-100 solution and 7% sodium deoxycholate for two times.In group II,another protocol was created with the detergents Triton X-200,sulfobetaine-16,and sulfobetaine-10 for two times. The degrees of decellularization,activity of laminin,degrees of demyelination,and integrity of the nerve fiber tube were observed under microscope and were assessed by a scoring system.[Results]In both experimental groups the activity of laminin was present and the degrees of decellularization were complete. As for the demyelization of the nerve segments,the myelin sheath in Group II was partially preserved,but it completely disappeared in Group Ⅰ. The structure of the nerve fiber tube in Groups Ⅰ and Ⅱ were not as integral as that in the normal group.[Conclusion]It may be a better method for the larger CANA,to be treated with TritonX-100 and sodium deoxycholate during the decellularization procedure.
5.Treatment of brain injuries in the acute phase
Liqing WANG ; Yu WANG ; Xin ZHANG
Chinese Journal of Rehabilitation Theory and Practice 2008;14(7):642-643
在地震中,颅脑外伤极为常见,其救治过程特点为:发生突然、时间急、伤员多、伤情复杂、抢救难度大。治疗细节决定救治的成败。在早期治疗中,主要是维持生命体征,为原发性脑损伤提供恢复条件,避免继发性脑损伤。尽早转运到后方专科医院治疗,减少死亡及伤残率。
7.Relationship between homocysteine and the levels of plasma folic acid, vitamin B_(12) and lipid in patients with acute cerebral infarction
International Journal of Cerebrovascular Diseases 2009;17(11):836-839
Objective To investigate the differences of the plasma homocysteine (Hcy) between the patients with acute cerebral infarction and the normal controls, and the relationship between the levels of plasma Hcy and folic acid, vitamin B_(12) and lipids, and the relationship between clinical symptoms in patients with acute cerebral infarction. Methods A total of 91 patients with first-ever acute cerebral infarction and 100 controls without cerebrovascular accident were included. Their ratio factors such as age, sex, hypertension and diabetes did not have significant differences. Enzymatic cycling was used to detect plasma Hcy levels. Chemiluminescence was use to detect folic acid and vitamin B_(12) levels. Biochemical analyzer was used to measure lipid levels. The patients with acute cerebral infarction were evaluated by the National Institutes of Health Stroke Scale (NIHSS). Results The plasma Hcy level was significant higher in the acute cerebral infarction group than that in the control group (21.22 ±7.29 μmol/L vs. 13.19 ± 2.13 μmol/L) (P < 0.05); the plasma Hcy level in the acute cerebral infarction group was significantly negatively correlated with folic acid (r = - 0.307,P <0.05) and vitamin B_(12) (r = - 0.270, P <0.05). It was significantly positively correlated with low-density lipoprotein (r =0.282, P <0.05), and it was significantly negatively correlated with high-density lipoprotein (r = -0.219, P <0.05). The mean value of the plasma Hcy in acute cerebral infarction group increased with the increase of NIHSS scores. Conclusions Hyperhomocysteinemia is an independent risk factor for acute cerebral infarction. The levels of folic acid and vitamin B_(12) decreased with the increase of Hcy levels. The higher the Hcy levels,the more serious the clinical symptoms are. Hcy may increase the risk of ischemic cerebrovascular disease by influencing the lipid metabolism.
8.Naturalistic observation of the effect of donepezil on cognitive function in Alzheimer's disease in memory clinic
Kai LIN ; Xin YU ; Huali WANG
Clinical Medicine of China 2009;25(4):345-347
Objective To examine the effect of donepezil on cognitive function of Alzheimer's disease from a naturalistic observation in memory clinic.Methods From the subject pool in memory clinic,thirteen patients with mild to moderate Alzheimer's disease(meeting NINCDS-ADRDA criteria of probable AD)were enrolled in the naturalistic observation.Donepezil with dosage of 5 mg daily Was administered.Cognitive Ability Screening Instrument (CASI)Was used to assess the cognitive function,and was administered at baseline,3 and 6 months after treatment with donepezil.Resuits After 6-month treatment with donepezil,the change of score of verbal fluency from baseline Was significant[(4.3±3.1)vs(6.2±2.7),P<0.05].There was a trend that constructional praxis was also improved after 6-month treatment(P=0.06).The change of scores of other cognitive domains remained the sanle(P>0.05).Conclusion In the naturalistic observation at memory chnic,donepezil may improve verbal fluency of AD patients,and keep most cognitive function stable.
9.A further discussion on acupuncture treatment plan of facial neuritis in Evidence-based Guidelines of Clinical Practice in Acupuncture and Moxibustion.
Chinese Acupuncture & Moxibustion 2014;34(6):602-604
The treatment plan of facial neuritis in Evidence-based Guidelines of Clinical Practice in Acupuncture and Moxibustion (2011 edition) is discussed, and case information of facial neuritis during the recent five years in department of acupuncture and moxibustion, PLA General Hospital, is retrospectively analyzed. In accordance with anatomy of the facial nerve to form the acupuncture prescription, the detailed diagnosis and treatment method for facial neuritis are introduced. The advantages of the diagnosis and treatment method for facial neuritis are summarized, hoping to establish a more comprehensive, standardized and unified treatment plan.
Acupuncture Points
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Acupuncture Therapy
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standards
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Evidence-Based Medicine
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standards
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Facial Nerve
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anatomy & histology
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Facial Nerve Diseases
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therapy
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Humans
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Moxibustion
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standards
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Practice Guidelines as Topic
10.Oral aspirin caused Reye's syndrome in a child.
Chinese Journal of Pediatrics 2004;42(4):243-243
Administration, Oral
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Anti-Inflammatory Agents, Non-Steroidal
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adverse effects
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therapeutic use
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Aspirin
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adverse effects
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therapeutic use
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Brain Edema
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chemically induced
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Child
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Coma
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chemically induced
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Female
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Humans
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Liver
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drug effects
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pathology
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physiopathology
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Reye Syndrome
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chemically induced
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Treatment Outcome