Age Factors ; Environmental Exposure ; Humans ; Neoplasms ; epidemiology ; prevention & control ; Research

OBJECTIVE:To explore the teaching methods for pharmacognosy course in order to improve the quality of teach-ing. METHODS:In view of the disadvantages of the traditional teaching method,the teaching methods in pharmacognosy courses were reformed and practiced in many ways. RESULTS:Special seminar was set up,the role of teacher and student exchange was applied,traditional culture and knowledge was penetrated in class teaching,extracurricular activities were carried out and field prac-tice was added. CONCLUSIONS:Through the reform practise of the teaching method in pharmacognostic courses in pharmacy ma-jor in our university,the initiative and enthusiasm of students' learning has been improved,the ability of professional knowledge has been expanded,innovation and practical ability have been actively cultivated and good teaching effected has achieved. The quali-ty of the teaching in pharmacognosy has been greatly improved.

Objective: To investigate the enhancing effect of ?-interferon on anticancer effect of tamoxifen against breast cancer cells in vitro. Methods: ER-positive MCF-7 and ER-negative MDA-MB-231 breast cancer cell lines were treated with tamoxifen alone, or in combination with ?-interferon or estrogen in vitro. Cell proliferation was evaluated by MTT assay; distribution of cell cycle was determined by flow cytometry( FCM) and cell apoptosis was determined by DNA gel electrophoresis and FCM. Results: Tamoxifen inhibited the growth of ER-negtive and-positive brest cancer cells, induced G0/G1 phase arrest and induced cell apoptosis. Tamoxifen at same concentration had stronger inhibitory effect on ER-positive cells than ER-negative ones. Promoting effect of estrogen on MCF-7 growth was blocked by TAM, but inhibition of MDA-MB-231 by TAM was not related to estrogen. Anticancer effect of TAM was enhanced when cells were pre-treated with ?-interferon for 24 h. Conclusion: TAM exerts its anti-cancer effect through inhibiting proliferation and inducing apoptosis of ER-positive/negative breast cancer cells in vitro, and ?-interferon can enhance this manticancer effect of TAM on breast cancer cells.

Acute Disease ; Child ; Child, Preschool ; China ; epidemiology ; Female ; Human bocavirus ; isolation & purification ; Humans ; Infant ; Infant, Newborn ; Male ; Respiratory Tract Infections ; epidemiology ; virology

OBJECTIVE: To observe the effects of Ginkgolides inhalation on eosinophils infiltration in airway of asthmatic guinea pig model. METHODS: Guinea pigs were divided into seven groups(n=8 in each group): model group, ginkgolides inhalation low, medium and high dosage groups(5, 10, 20mg?kg-1, respectively), ginkgolides ip group (at a dosage of 20 mg?kg-1), Dexamethasone ip group (at a dosage of 10 mg?kg-1), normal control group. The eosinophils (EOS) in bronchoalveolar lavage fluid (BALF) and trachea were counted. RESULTS: As compared with normal control group, the number of EOS in BALF was significantly increased in model group, but that in BALF and trachea of the Ginkgolides inhalation group was significantly reduced(P

Objective To evaluate anesthesia efficacy of sevoflurane combined midazolam and remifentanil and propofol in patients undergoing short surgery. Methods Fifty ASA Ⅰ or Ⅱ patients who scheduled for elective surgery under general anesthesia were enrolled in study. The anesthesia was induced with intravenous injection of midazolam 0.03 mg/kg and sevoflurane inhalation. Induction of anesthesia was started with 6% sevoflurane and fresh gas flow rate of 6 L/min. The inspired concentration of sevoflurane was reduced to 2% as soon as eyelash reflexes of these patients were abolished. At the same time, remifentanil 2 μg/kg was injected slowly over 1 min,and 30 s later,patients were mechanically ventilated after tracheal intubation. 2% sevoflurane was kept inhaling, and remifentanil and propofol were kept pumping for anesthesia maintenance. The mean arterial pressure (MAP), heart rate, pulse oxygen saturation (SpO_2) and bispectral index (BIS)were recorded before administration,at the time of the disappearance of eyelash reflex, immediately before tracheal intubation,and immediately after tracheal intubation. The time period from sevoflurane inhalation to the disappearance of eyelash reflex was also recorded. And conscious recovery, spontaneous breathing recovery,the endotracheal extubafion time and OAAS score were recorded. Results All patients were successfully intubated at the first attempt. The satisfactory intubation conditions were found in 82% (41/50) of cases. The time period from sevoflurane inhalation to the disappearance of eyelash reflex was (73 ± 12) s. MAP,heart rate and SpO_2 remained stable and BIS was maintained at 45-55 before and after tracheal intubation, and all patients were quick recovery. Conclusion The anesthesia efficacy of sevoflurane combined midazolam and remifentanil and propofol without muscle relaxant in patients undergoing short surgery is smooth, safe and reliable.

Objective To observe the effects of ginkgolides on hypoxia tolerance in mice and on myocardial ischemia injury in rats.Methods The mice were given isoprenaline 20 mg/kg(ip) and the survival time of the mice model under the hypoxic condition was recorded. Rat myocardial ischemia was induced by subcutaneous injection of over- dosage isoprenaline(8 mg/kg). Before modeling, rats were pretreated with ginkgolides 10, 20, 40 mg/kg, of ginkgo biloba( EGb) Extract 300 mg/kg, Propranolol 5 mg/kg,or vehicle for four days. The histological changes of myocardiim, serum creatine phosphokinase (CPK) level and lactate dehydrogenase (LDH) activity, myocardial lactate dehydrogenase(LDH) and superoxide dismutase (SOD) content were detected.Results Ginkgolides could prolong the survival time in the mice under a hypoxic condition, lessen the isoprenaline- induced rat myocardial ischemia, inhibit the activities of serum CPK and LDH and the increase of MDA content in ischemic myocardial tissue, enhance superoxide dismutase (SOD) activity. Conclusion Ginkgolides enhance to hypoxia the tolerance in mice and prevent rats from myocardial ischemic injury. The protective mechanism may be related to its inhibition of the activity of platelet activating factor and oxygen free radical. 

Objective To study the relationship between hepatocellular apoptosis and glycogen contents during hepatic cold preservation reperfusion and its mechanism.Methods Based on the model of four groups of rabbit livers with different hepatocellular glycogen contents, hepatocellular apoptosis and bax gene expression were observed during hepatic cold preservation reperfusion.Results Apoptotic hepatocytes were obviously found in 60 minute reperfusing livers subsequent to 9 hour cold storage, and there was significant difference in the numbers of apoptotic hepatocytes among all the groups. In the same time, there was the close relationship between the levels of bax gene expression and the glycogen contents of hepatocytes.Conclusion Intracellular abundant glycogen may significantly depress the hepatocellular apoptosis during hepatic cold preservation reperfusion by decreasing hepatocellular bax gene expression.

Aim To observe the effect of kanamycin on the change of Ca2+ concentration in outer hair cells caused by acetylcholine to explore the relationship between kanamycin ototoxicity and the cochlear efferent nervous system.Methods After the guinea pigs were killed by decapitation,the OHCs were isolated in artificial perilymph and divided into three groups:control group,acetylcholine(ACh) group and kanamycin + ACh group.OHCs were loaded with Fluo-3/AM for 30 min and then the change of Ca2+ fluorescence intensity was observed.Results Ca2+ fluorescent intensity in OHCs remained basically unchanged in control group,increased 3.06 times(n=6) in ACh Group,and increased 1.75 times(n=6) in kanamycin +ACh Group.Conclusions Kanamycin can obviously inhibit the increasing of Ca2+ concentration in OHCs caused by ACh.