Objective To evaluate the clinical application and significance of enzyme-linked immunosorbent assay(ELISA)for serologically diagnosing bullous dermatosis.Methods Serum Dsg1,Dsg3 and BP180 expressions were detected in 34 cases of pem-phigus,32 cases of bullous pemphigoid and 33 cases of non-bullous dermatosis by ELISA.Results The sensitivity and specificity of ELISA for detecting the bullous dermatosis antibody were 87.88% and 93.94% respectively.Conclusion The ELISA method has the diagnostic value on bullous dermatosis and the important significance in the disease classification.

Objective To observe the application of endoscopy combined with 125I seed implantation in the pancreatic cancer palliative surgery in elderly patients.Methods A total of 78 elderly patients with pancreatic cancer who can not be treated with radical operation were divided into observation group and control group (n=39 each group).All patients were treated with stent intervention.Patients in the ation group were treated with 125I seed implantation guided by CT or ultrasonangraphy additionally.The clinical symptoms were compared between groups.The survival time and complications of the patients were recorded during the follow-up.Results The clinical symptoms of patients in both two groups were greatly improved (x2 =0.06,P＞0.05).Compared with the control group,the pain remission was better and the incidence of pain caused by pancreatic cancer was lower in the observation group (46.2％ vs.76.9％,x2 =7.80,P＜0.05).There was a significant difference in the average of survival time between the observation group and control group [(13.7±3.4) months vs.(8.4±3.8) months,t=3.73,P＜0.01],but no statistical difference was found in the long-term survival rate between groups.There was no significant difference in the incidence of complications between the two groups (10.3％ vs.7.7％,x2=0.15,P＞0.05).Conclusions Endoscopy combined with 125Ⅰ seed implantation is an effective treatment for elderly patients with advanced pancreatic cancer,which can effectively relieve the clinical symptoms,proiong the survival time,reduce the pain and complications.

Objective To investigate the clinical utility of dual-energy virtual non-contrast CT(VNCT)of dual source CT in the di-agnosis and differential diagnosis of solitary pulmonary nodule(SPN).Methods Thirty-six patients with suspected SPN underwent chest plain single energy CT and dual-phase contrast enhanced CT (DECT)(25 and 90 s).The images of dual energy were sent to a commercial workstation for VNC image generation.CT values of SPN on VNC and true non-contrast(TNC),as well as signal-to-noise ratio(SNR)and contrast-to-noise ratio(CNR)were compared.The accuracy of calcification detection was compared according to the diameter and density of the calcification.The TNC images were used as the reference.Results CT values,SNR and CNR of VNC had no statistical difference among TNC and VNC at 25 s and 90 s(P >0.05).DECT VNC(5mm slice)could accurate detected calci-fication(diameter>2 mm,CT value>1 50 HU)in SPN.The 2 mm slice performed significantly better than 5 mm slice VNC images. Conclusion The VNC could provide consistent diagnostic information with TNC.The thin slice DECT VNC images can be used in clinical practice to replace TNC without losing small calcification in SPN,which has potential to reduce the patient radiation dose.

Object To isolate and identify the flavones from the flower of Paulownia tomentosa (Thunb.) Steud., which has been used as traditonal herb medicine in the treatment of some diseases. Methods The flavones were isolated by column chromatography and their structures were elucidated through spectroscopic analysis (NMR). Results Five flavones were isolated and identified as 5, 4′-dihydroxy-7, 3′-dimenthoxyflavanone (Ⅰ), 5-hydroxy-7, 3′, 4′-trimenthoxyflavanone (Ⅱ), diplacone (Ⅲ), mimulone (Ⅳ) and apigenin (Ⅴ). Conclusion All these compounds are obtained from the flower for the first time and the flavones should be as bioactive constituents.

Objective: To study the pharmacological action and clinical curative effect of Linglongqingre microenema. Methods: The actions of relieving fever and calming hypnotism. anti convulsions of Linglongqingre Microenema on rabbit, mouse and rat were observed. Also children's fever illess was treated to judge preliminary curative effect. Results: Linglongqingre Microenema has obvious action of relieving fever, more effective than Chaihu injection, as same as Antipyrine (80mg/kg) on subcutaneous injection. It was used to treat children's feverillness on 38 cases, markedly effective rate 60.53%, total effective rate 86.84%. Among them body temperature of one third case fell over 1.5?C in two hours. Conclusion: Linglongqingre microenema is a new quick effective pharmaceutical preparation which is suitable for emergency treatment of traditional Chinese medicine

Objective To study the influence of carboxymethyl-chitosan(CM-chitosan)on chondro- cyte apoptosis induced by recombinant human interleukin-1?(rhIL-1?)and explore its mechanism.Methods Rabbit chondrocytes were isolated and cultured.Chondrocytes were pretreated with different concentrations of CM-chitosan for 1 h,then 10 ng/ml IL-1?were added into the culture medium.After 24 h,the apoptotic rates of chondrocytes were measured by flow cytometry with AnnexinⅤ-FITC and PI staining.The morphology of nuclei was observed by fluorescent microscopy with Hoechst 33342 staining.The mitochondrial membrane po- tentials were tested by confocal laser scanning microsocopy with Rhodamine-123 and ATP contents were mea- sured by luciferase reaction.Results CM-chitosan could inhibit chondrocyte apoptosis and restore the func- tion of mitochondria induced by IL-113 in a dose-dependent manner.Conclusion CM-ehitosan can inhibit chondrocyte apoptosis by protecting mitochondrial function.

Whennearinfraredspectroscopyisappliedtoon-linemonitoringandcontroloftobaccoflavors,the variation in temperature can severely deteriorate the predictive performance of near infrared spectroscopic calibration models and results in a significant increases of the root mean square error value for the main constituents in syrup samples from 2. 4% to 29. 0%. In this paper, near infrared spectroscopy has been incorporated with an advanced calibration transfer method-loading space standardization to effectively eliminate the deteriorate effects of temperature variation on quantitative results and finally realize the fast and accurate on-line quantitative monitoring and control of tobacco flavors. The root mean square error value for the main constituents in syrup samples is successfully retained at a satisfying low level of 3 . 8%. The results of this paper will provide technical support for the preparation, preservation and use of tobacco flavors, and realize on-line process quality control of cigarettes.

Objective To compare and quantify the determinants in quantitative electroencephalogram(q-EEG) and heart rate variability power spectrum analysis(HRV-PSA) of ketamme(KTM) anesthesia for preschoolers. Methods Seventy four cases were selected and assigned into 3 groups named A(4-5 years), B(5-6 years), C(6-7 years), 22,28,24 cases in every group respectively. All cases were induced with KTM 5 mg /kg intramuscularly and changes of determinants were recorded continuously. If body movement happened, KTM would be injected with 1 mg/kg. Results On pre- anesthesia, BIS in group A was the least among 3 groups, while LF/HF and HRVI were the largest(P

Objective To explore the mechanisms by which hydrogen sulfide (H2S) regulates collagen metabolism during hypoxic pulmonary vascular structural remodeling. Methods Wistar rats were randomly divided into control group (n=6), hypoxia group (n = 6) and hypoxia+NaHS group(n= 6) H2S content in plasma was measured with spectrophotometry. The expression of collagen I was detected by immunohistochemistry. The expression of tissue inhibitor of metalloproteinase-1(TIMP-1) mRNA was detected with situ hybridization Results Compared with hypoxia group, H2S content in plasma increased significantly and the expressions of collagen I and TIMP-1 mRNA in pulmonary arteries were down- regulated in hypoxia + NaHS group. The H2S content in plasma negatively correlated with TIMP-I mRNA expression. Conclusions H2S increases the degradation of collagen in small and media pulmonary arteries of rats under hypoxia by decreasing the synthesis of TIMP-1, and therefore, attenuats the collagen remodeling in pulmonary arteries of hypoxic rats.

Objective To evaluate the efficacy of left ventricular ejection time (LVET) in guiding the volume management during liver transplantation.Methods Sixty American Society of Anesthesiologists physical status Ⅱ or Ⅲ patients,aged 32-64 yr,weighing 54-93 kg,of Child-Pugh grade A or B liver function,scheduled for elective the first liver transplantation with general anesthesia,were divided into either control group (group C) or transesophageal echocardiography (TEE) monitoring group (group TEE),with 30 patients in each group.In group C,the fluctuating range of mean arterial pressure,heart rate and central venous pressure was maintained less than 20％ of the baseline value,and the urine volume was maintained ＞1 ml · kg-1 · h-1.LVET was maintained between 0.35-0.40 s in group TEE.The consumption of intraoperative vasoactive agents (dopamine,norepinephrine,epinephrine),volume of fluid infused,volume of blood transfused,blood loss and urine volume were recorded.The occurrence of adverse events was observed during the perioperative period,and postoperative extubation time and intensive care unit residence time were also recorded.Results Compared with group C,the consumption of intraoperative dopamine and norepinephrine was significantly decreased,the urine volume was increased,the incidence of myocardial ischemia,pulmonary edema and renal failure in the perioperative period was decreased,and the postoperative extubation time and intensive care unit residence time were shortened in group TEE (P＜0.05).There was no significant difference between the two groups in the volume of crystalloid,colloid,red blood cells and plasma infused or blood loss (P＞0.05).Conclusion LVET produces good efficacy in guiding the volume management during liver transplantation.