Objective In order to improve the survival of graft liver after liver transplantation, this study was designed to investigate whether intraportal injection of 150mg/kg N-acetylcysteine (NAC) in rats could reduce hepatic ischemia-reperfusion injury after 48 h of cold storage and 2 h of reperfusion.Methods Healthy male Wistar rats weighing 250-350g were used.The study consisted of three groups: control group (group Ⅰ);NAC-treated group(group Ⅱ).1 ml of 5% dextrose (D5%) or 1 ml D5% containing 150mg/kg NAC was injected into the superior mesenteric vein.15 min after the injection of D5% or NAC the liver was flushed with cold (4℃) Ringer's solution through the portal vein .After perfusion, the liver was removed and kept in 100 ml UW solution at 4℃ for 48 h.In group Ⅲ animals were pretreated with buthionine sulfoximine (BSO) 2 h before intraportal injection of D5% or NAC and liver harvesting.After cold storage, the livers were then perfused for 2 h by a closed circulating system.Aspartate transaminase (AST), alanine transaminase (ALT), and lactate dehydrogenase (LDH) activities in the perfusate were determined by reflectometry.Lactate and acid phosphatase activities were determined by enzymatic methods.Results After 48 h of cold storage and 2 h of reperfusion, livers from NAC-treated group produced larger amounts of bile than those in the control group, and released less LDH, AST, ALT and acid phosphatase, a marker of Kupffer cell injury in the perfusate.The protective effects of NAC against cold ischemia-reperfusion liver injury were maintained when animals were pretreated with BSO, a specific inhibitor of glutathione synthesis.Conclusions This study shows that intraportal administration of NAC in vivo significantly improves the initial function of the isolated rat liver.Our results also indicate that NAC inhibits the activation of Kupffer cells, which are the first source of reactive oxygen intermediates during reperfusion.

ObjectiveIn order to improve the survival of graft liver after liver transplantation, this study was designed to investigate whether intraportal injection of 150mg/kg N-acetylcysteine (NAC) in rats could reduce hepatic ischemia-reperfusion injury after 48 h of cold storage and 2 h of reperfusion. Methods Healthy male Wistar rats weighing 250-350g were used. The study consisted of three groups: control group (group Ⅰ) ;NAC-treated group(group Ⅱ). 1 ml of 5% dextrose (D5%) or 1 ml D5% containing 150mg/ kg NAC was injected into the superior mesenteric vein. 15 min after the injection of D5 % or NAC the liver was flushed with cold (4℃) Ringer' s solution through the portal vein . After perfusion, the liver was removed and kept in 100 ml UW solution at 4℃ for 48 h. In group Ⅲ animals were pretreated with buthionine sulfoximine (BSO) 2 h before intraportal injection of D5 % or NAC and liver harvesting. After cold storage, the livers were then perfused for 2 h by a closed circulating system. Aspartate transaminase (AST), alanine transaminase (ALT), and lactate dehydrogenase (LDH) activities in the perfusate were determined by reflectometry. Lactate and acid phosphatase activities were determined by enzymatic methods. ResultsAfter 48 h of cold storage and 2 h of reperfusion, livers from NAC-treated group produced larger amounts of bile than those in the control group, and released less LDH, AST, ALT and acid phosphatase, a marker of Kupffer cell injury in the perfusate. The protective effects of NAC against cold ischemia-reperfusion liver injury were maintained when animals were pretreated with BSO, a specific inhibitor of glutathione synthesis. ConclusionsThis study shows that intraportal administration of NAC in vivo significantly improves the initial function of the isolated rat liver. Our results also indicate that NAC inhibits the activation of Kupffer cells, which are the first source of reactive oxygen intermediates during reperfusion.

Objective To investigate the effect of different concentrations of heparin in the cold preservation solution on blood coagulation after reperfusion during orthotopic liver transplantation ( OLT) . Methods Forty ASA Ⅱ or Ⅲ patients with end-stage liver disease (31 males, 9 females) weighing 51-82 kg undergoing OLT were randomly divided into 3 groups according to the concentrations of heparin in the liver preservation solution: group Ⅰ 2 000 U?L-1( n = 14); group Ⅱ 8000 U?L-1(n= 12) and group Ⅲ 20 000 U?L-1(n = 12) .Anesthesia was induced with propofol, fentanyl and rocuronium and maintained with isoflurane inhalation and intermittent i.v. boluses of fentanyl. Body temperature was maintained at 35.5-37.5℃ and Hct at 22%-35% . Blood samples were taken from central vein at 15 min before reperfusion (T0,baseline) and 5, 30, 60, 120 and 180 min of reperfusion (T1-5) for determination of ACT, CR and platelet function (PF) using Sonoclot platelet function and coagulation analyzer (U.S.A.). Blood concentration of heparin was determined in 3-4 patients in each group at T0-5 ( HPLC) . Results The 3 groups were comparable with respect to age, body weight, ASA physical status and Child classification. The glass ball activated coagulation time (gbACT) was significantly prolonged at T1-4 in group Ⅲ and at T1,2 in group Ⅱ as compared to the baseline values at T0 ( P

Chinese medical records recorded the whole activities during a syndrome differentiation and treatment of disease. With the characteristics of diversity and lively, the records have important values in establishing TCM therapeutic thoughts and abilities for students. Amount of medical records have been recorded in medical books since the Western Han dynasty. In order to provide the references for teaching prescription aided by medical records, this article discusses the choice and application of medical records to teach prescription by listing examples on the basis of outlining the development of TCM medical records.

Objective To set up a simple method to measure the SR Ca2+ release function in skeletal muscle strips.Methods The time from peak tension to 75 % relaxation(TR75) during intermittent tetanic contraction(fatigue contraction) of isolated musculus soleus strips was analyzed.It was prolonged rapidly,and then shortened slowly.The ratio of maximal TR75 to initial TR75(R-TR75) indicated a balance between SR Ca2+ release and uptake.If the SR Ca2+-ATPase(SERCA) activity and inhibition extent of SERCA in fatigue contraction were identical,R-TR75 should be an index for SR Ca2+ release function.Results Higher stimulation voltage or 5 mmol/L caffeine perfusion which increased the SR Ca2+ release channel open probability induced an increase of R-TR75 from 2.5 in control to 3.0.On the contrary,inhibition of SR Ca2+ release with 5 mmol/L magnesium sulfate perfusion significantly decreased R-TR75.Inhibition of SR Ca2+ release by metabolites during fatigue contraction was recovered slowly.So R-TR75 at the second fatigued contraction was reduced after 5 or 10 min of recovery,but no significant difference after 60 min of recovery.During intermittent tetanic contraction R-TR75 was increased to be 2.9 times of normal in unloaded soleus.Conclusion The above results suggest that R-TR75 during muscle strip fatigue contraction without any change of SERCA activity can indirectly reflected SR Ca2+ release function.In 2-week unloaded soleus,the enhanced R-TR75 indicates an increase in SR Ca2+ release function.

Objective To determine whether thiol can affect the activity of nuclear factor kappa B (NF-?B) in and release of TNF-?from lipopolysaccharide (LPS)-activated kupffer cells (KCs) and ascertain whether these effects are mediated through glutathione (GSH) .Methods KCs were isolated from livers of healthy male adult SD rats weighing 250-300g and cultured for 12h. The cultured KCs were exposed to LPS 10 ng?ml-1. The effects of different concentrations of glutathione monoethylester (GSHEE) and N-acetylcysteine (NAC) on NF-?B activity in and release of TNF-afrom KCs were determined. The effect of pretreatment with GSH synthesis inhibitor - BSO on the inhibitory effect of NAC on inflammation was investigated. NF?B activity was determined by electrophoretic mobility shift assay (EMSA) and intracellular GSH content and TNF-?level in supernatant by HPLC. Results Intracellular GSH content remained unchanged after exposure of KCs to LPS. Both GSHEE and NAC increased intracellular GSH level and inhibited NF-?B activity and release of TNF-?. BSO blocked the increase in GSH induced by NAC but did not affect the inhibitory effect of NAC on TNF?release. Conclusions LPS does not affect GSH level in KCs. NAC can inhibit NF-?B activity in and TNF-?release from KCs and increase intracellular GSH level but the inhibitory effect is not mediated through GSH.

Objective To study the toxicity of benzene combining with formaldehyde on reproductive cells in mice.Methods Male mice were randomly divided into different groups:three experimental groups with different doses of benzene combining with formaldehyde(mg?kg~(-1))(10+10,50+50 and 100+100),control group and vegetable oil group(n=10).After 8 weeks by oral poison,the mice were killed,testis was weighed,sperm count was peformed,the rate of sperm deformation was counted and the SOD activity was measured.Results The quantities of sperm in various poison groups were obvious decreased and the rate of sperm deformation were obvious increased with dose increasing.Compared with control group,they both had obvious difference(P

Objective To observe the clinical efficacy of triple needling at myofascial trigger points plus warm needling in treating periarthritis of shoulder. Method Eighty patients with periarthritis of shoulder were randomized into a treatment group of 44 cases and a control group of 40 cases. The treatment group was intervened by triple needling at myofascial trigger points plus warm needling, while the control group was by warm needling alone. The two groups were treated once a day, 10 times as a treatment course. The Pain Rating Index (PRI), Visual Analogue Scale (VAS), Present Pain Intensity (PPI), and motion of shoulder joint were observed before the treatment and after 2 treatment courses, and the clinical efficacies were compared between the two groups.Result In the treatment group, the PRI, VAS, and PPI scores respectively after 1 and 2 treatment courses were significantly different from those before the treatment (P<0.05,P<0.01). In the control group, the scores after 2 treatment courses were significantly different from those before the treatment (P<0.05). After 2 treatment courses, the scores in the treatment group were significantly different from those in the control group (P<0.01). In both groups, the motions of shoulder joint respectively after 1 and 2 treatment courses were significantly changed compared with those before the treatment (P<0.01). The motions of shoulder joint in the treatment group were significantly different from those in the control group respectively after 1 and 2 treatment courses (P<0.05,P<0.01). The recovery plus markedly-effective rate and total effective rate were respectively 70.5% and 95.5% in the treatment group, versus 52.5% and 90.0% in the control group, and the between-group differences were statistically significant (P<0.01,P<0.05).Conclusion Triple needling at myofascial trigger points plus warm needling is an effective approach in treating periarthritis of shoulder.

Objective To explore the long-term efficacy of botulinum toxin type A injections on facial beauty and its long-term safety.Methods A total of 33 beauty-seekers with botulinum toxin type A treatment for more than five years were reviewed as observation group,using digital muscle palpation meter (Myoton PRO) for the determination of the orbicularis oculi muscle and masseter muscle tension (F),the muscle characteristic parameters,such as muscle hardness (S) using a homemade facial questionnaire test for satisfactory rate of beauty from both beauty-seekers and physicians.At sametime,33 normal adults that never accepted botulinum toxin injection with matched age and gender were collected as control group.The same-sex indicators were dtermined and compared with using statistic analysis t test.Results The pairwised parameters of the same sex and site were comparied between the two groups;average F and S values in the observation group were lower than those of the control group,but no statistically significant difference were observed between the two groups (P＞0.05);in the observation group,average appearance age was 7.3 years younger than the control group,and the facial shape improved significantly.Conclusions Long-term and repeated application of botulinum toxin A is able to remove crow's feet and decrease the masseter and so the injection is safe with high satisfaction to beauty-seekers.

AIM:To analyze the effect 17?-estradiol on activated macrophages and possibility of being a target of screening drugs for endometriosis. METHODS: Using scanning electron microscope, the surface of macrophages was observed. Nitric oxide (NO) in supernatant was determined by Griess's reagent. TNF-? was measured by MTT via L929 cells. Using Fluo-3/AM, i was measured by laser scanning confocal microscopy (LSCM). RESULTS: (1) Increased size, extension of more microvilli, expression of retraction fibers and elaboration of membrane ruffles were detected in 17?-estradiol treated macrophages. (2) 17?-estradiol induced NO release from peritoneal macrophages. (3)The more TNF-? was made by macrophages under the induction of 17?-estradiol. (4)Intracellular Ca 2+ elevated 39.8% in peritoneal macrophages after 17?-estradiol (100 nmol/L) treatment. CONCLUSIONS: Macrophages were activated by 17?-estradiol at a certain concentration. Activated macrophages by 17?-estradiol may participate in endometriosis and may be a target of screening drugs for endometriosis.