This study proposed to use Term Frequency - Inverse Document Frequency (TF-IDF) relative entropy as knowledge representation method between symptoms and syndrome. TF-IDF was originated from text mining. It was an important method in the automatic text categorization. TF-IDF also represented the automatic categorization idea in traditional Chinese medicine (TCM) syndrome. It was based on the fact that the higher frequency of one symptom in specific syndrome, the stronger ability to distinguish this syndrome (TF); and the more wide range of one symptom in all syndrome, and the lower ability to distinguish a syndrome (IDF). It was verified with specific examples.

High-level researchers,who can efficiently enhance the research strength of the college,are often brought in at high cost.Therefore there is often the need to scientifically evaluate these researchers when introduce them.We here discuss the main indicators for the evaluation of High-level researchers.Taking into consideration of their age,research span,et al.,we propose a model for the quantitative evaluation of research capability based on research achievement and prediction of future achievement,

The nanoparticle-based electrochemical technology for early detection of cancer is an imjportant research topic in the area of biomedicine.This article introduces the concept of tumor marker and principle of electrochemical detection of the tumor marker.The applications of nanoparticles in electrochemical early detection of cancer are reviewed in detail.Finally,the prospected application of research is discussed.

Aged ; Ear, Middle ; Eustachian Tube ; Female ; Foreign Bodies ; Humans

0.05).After exposure of U937 macrophages to 11.1 mmol/L,16.7 mmol/L and 33.3mmol/L glucose,the expression of LOX-1 protein was 1.56-,2.26-and 2.96-fold,as compared with that of 5.6 mmol/L glucose group(P

BACKGROUND:How to preserve the fertility and ovarian function in patients with procreation needs has important significance and broad application prospects. OBJECTIVE:To summarize the recent studies about ovarian tissue cryopreservation, preservation and evaluation methods, and to explore the best solution for ovarian tissue freezing. METHODS:An online computer-based retrieval of PubMed database and Wanfang database between January 1994 and January 2014 was performed by the first author. The key words were ovarian tissue, ovarian tissue cryopreservation, freezing factors in English and Chinese. Final y 59 literatures on the ovarian tissue cryopreservation technique, influencing factor of cryopreservation, resuscitation, and human ovarian tissue transplantation were included. RESULTS AND CONCLUSION:Currently, laboratory methods of ovarian tissue cryopreservation are divided into slow-rate freezing, ultra-rapid cooling and vitrification technology. The freezing effect may vary in different patients, tissue sections, cryoprotectants species, penetration time and frozen carrier. After the recovery, the histological morphology of granulosa cells and fol icles was observed. The fol icles were counted to evaluate ovarian tissue. Freeze-thaw effects were evaluated by observing the changes of subcellular structures. The apoptotic signals were also detected. Immunohistochemical analysis was applied to detect the proliferation after recovery and apoptosis-related information. Variation in the in vitro culture reflected endocrine levels. Genetic level detection was also performed. In recent years, frozen ovarian tissue is clinical y applied for the transplantation, to restore reproductive endocrine function of the patients, or get mature germ cells, thereby preserving female fertility. However, there are stil some questions about cryopreservation of ovarian tissue, which need further study.

Objective To explore the effects of the meteorologic factors on air pollutants and to establish a model of relationship between the meteorologic factors and concentration of air pollutants. Method The data of SO2,NO2 and PM10 concentration in the air and the related meteorologic factors (daily average,maximum and minimum atmospheric pressure and daily atmospheric pressure difference,daily average,maximum and minimum air temperature,monthly rainfall,daily average and minimum relative humidity and wind speed) from January 2002 to December 2007 were collected. The correlation between the air pollutant levels and related meteorologic factors was analyzed by the multiple regression models with SPSS 11.5 software and multiple regression equations were established. Results Minimum relative humidity was negatively correlated with the concentration of SO2 and NO2 in atmosphere . Daily minimum air temperature was negatively correlated with the concentration of NO2 in atmosphere. Daily average relative humidity and wind speed were negatively correlated with the concentration of PM10 in atmosphere. Daily atmospheric pressure difference was positively correlated with the concentration of PM10 in atmosphere. Multiple regression equations of the concentration of SO2,NO2 and PM10 in atmosphere and meteorologic factors have statistical significances. Conclusion The meteorologic factors have some effects on the air pollution levels. By using the correlation of meteorologic factors and the concentration of air pollutions,multiple regression equations can be established.

Objective To analyze time-space variation of air pollutants(SO2,NO2,PM10) and the correlation among them in inner region and outer region of Shenzhen from 2002 to 2007.Methods The daily concentration means of SO2,NO2 and PM10 during 2002 to 2007 from the nine environmental monitoring sites in Shenzhen were collected.The determination was conducted according to the Ambient Air Quality Criteria.Results From January to December,variation of air concentration of SO2,NO2 and PM10 met the "V" shape in inner region and outer region of Shenzhen in 2002-2007,which was descending from January to June and ascending from June to December.The level of the air pollutants in inner region and outer region was higher in fall and lower in summer.The air pollution level of the inner region was increasing from 2002 to 2004,then decreased in 2005,but increased again in 2006 and 2007.The tendency of the outer region was similar with the tendency of the inner region from 2002 to 2006,but the level of pollutants decreased in 2007.The air concentration of SO2 in outer region was higher than that in inner region in 2002-2007.The air concentration of NO2 and PM10 in outer region was higher than that in inner region in 2002 to 2006,but lower in 2007.The air concentration of SO2 in inner region and outer region in 2002-2007 was under the limit of grade 2 according to the GB 3095-1996,near to grade 1 in inner region.The air concentration of NO2 and PM10 was under the limit of grade 3 and grade 2 respectively.There was a close correlation among the levels of SO2,NO2,PM10 in Shenzhen.Conclusion The air quality in Shenzhen is better in China.The time-space variation of the air pollutants is obvious and a close correlation exists among the air pollutants.

[Objective]Electrophoretic analysis of rat chemically extracted acellular nerve allograft(CEAN) protein was performed to investigate the protein of CEAN.Dorsal root ganglia of chicken were cultured onto the surface of CEAN slice to observe the effect of CEAN structure on the fiber regeneration.[Method]The CEAN were perpared according to Sondell method and electrophoresed to observed whether the electrophoresis strip of 28-30kDa(Myelin Protein) exited or not.Dorsal root ganglia of chicken were cultured onto thc surface of CEAN slice and dyed with nerve fiber fluorescence to observe the orientation of nerve fiber growth on the surface of CEAN slice.[Result]Electrophoretic analysis of rat CEAN protein showed that the electrophoresis strip of 28-30 kDa totally disappeared.A large number of nerve fibers grew from DRG along the basement membrane of CEAN.[Conclusion]No myelin protein remained in the CEAN,while the basement membrane structure of CEAN can induce the nerve fiber to grow.

[Objective] To investigate the biological characteristics of Wharton’[KG-38x]s jelly-derived mesenchymal stem cells (WJMSCs) and their differentiation into Schwann cell-like cells, and to provide a new cell seed source for nerve tissue engineering or cell therapy. [Methods]Umbilical arteries, veins and umbilical cord tunica externa were removed , and the remaining tissue (Wharton’[KG-38x]s jelly) was harvested.The umbilical cord was cut into small fragments and cultured with DMEM in orderto gather. Cultured WJMSCs were confirmed by the detection of MSC-specific cell-surface markers. WJMSCs were differentiated along a glial cell lineage using an established cocktail of growth factors. Immunocytochemical staining and Western blot were used to evaluate the characteristics of differentiated WJMSCs.[Results]WJMSCs were immunonegative for the haemopoietic cell-surface markers (CD34 and CD45) and neural stem cell marker (nestin), and immunopositive for MSC surface markers CD44, CD105, Stro-1 and vimentin. WJMSCs treated with a mixture of glial growth factors adopted a spindle-like morphology similar to Schwann cells. Immunocytochemical staining and Western blot analysis revealed that the treated cells expressed the glial markers p75 and glial fibrillary acidic protein indicative of differentiation.[Conclusion]WJMSCs can be differentiated into the cells that are Schwann-like in terms of morphologic features and phenotype and may be suitable as Schwann-cell substitutes for nerve repair in clinical application.