2.Effect and mechanism of cadmium on progesterone synthesis in ovaries granule cells.
Hai-mei JIA ; Yu CHEN ; Jia-li WANG
Chinese Journal of Industrial Hygiene and Occupational Diseases 2010;28(3):213-216
Animals
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Cadmium
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toxicity
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Cytochrome P-450 Enzyme System
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blood
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Female
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Ovary
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drug effects
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metabolism
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Phosphoproteins
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blood
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Progesterone
;
biosynthesis
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Rats
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Rats, Wistar
3.Preparation Technology of Chinese Traditional Medicine Seborrhea Liniment
Yanfu DONG ; Mei QI ; Lilun YANG ; Lijun YU ; Hai JIA
China Pharmacy 2007;0(33):-
OBJECTIVE:To study the preparation technology of Chinese traditional medicine Seborrhea liniment.METHODS:The preparation technology of Seborrhea liniment was optimized by orthogonal experiment design L9(34)using 60% alcohol as solvent with the content of Berberine hydrochloride as index for evaluation and with the extraction method,extraction time,amount of solvent and number of extraction times as factors.RESULTS:The optimum preparation technical conditions were as follows:by impregnating method,the extraction time was 48 h using 1 500 mL alcohol and the extraction frequency was 2 times.CONCLUSION:The preparation technology of Chinese traditional medicine Seborrhea liniment is stable and feasible,and it serves as a theoretical basis for the industrial production.
4.Synthesis and anti-SARS-CoV-2 activity and mechanism research of lycorine derivatives
Yu-heng MEI ; Jia-yu LI ; Dan-qing SONG ; Zong-gen PENG ; Ying-hong LI
Acta Pharmaceutica Sinica 2024;59(2):395-403
We designed and synthesized eighteen lycorine derivatives with five different structural types, and evaluated their antiviral activities on a HCoV-OC43-infected H460 cell model. Structure-activity relationships suggested that the introduction of appropriate substituents on the 6N atom of lycorine was beneficial to activity. Compound
5.Influence of Naloxone on ?-Exdorphin and Calcitonin Gene-Related Peptide in Convulsive Children with Brain Damage
chun-mei, JIA ; dong-mei, WANG ; ya-nan, XIN ; qiao-lian, WANG ; jian-hui, ZHANG ; yu, WANG
Journal of Applied Clinical Pediatrics 2006;0(20):-
0.01),which were significantly different from group Ⅲ(P
6.A new eremophilane derivative from Ligularia intermedia.
Shu-Li LI ; Yu-Heng LIU ; Qiu-Juan HAO ; Xiang-Mei ZHANG ; Yue-Mei JIA ; Na LI
China Journal of Chinese Materia Medica 2014;39(12):2281-2283
A new eremophilane derivative, (3aR,4R,5S,7S,7aS)-2-acetyl-7,7a-dihydroxy-3a,4-dimethyl-3a,4,5,6,7,7a-hexahydro-3H-inden-5-yl acetate (1) and three known compounds, 10beta-hydroxy-eremophil-7 (11)-en-12,8alpha-olide(2), beta-sitosterol (3) and beta-daucosterol(4) have been isolated from Ligularia intermedia. The compounds were isolated by column chromatography on silica gel and Sephadex LH-20,and identified on the basis of spectral analyses (MS, 1H-NMR, 13C-NMR).
Asteraceae
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chemistry
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Drugs, Chinese Herbal
;
chemistry
;
isolation & purification
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Molecular Structure
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Naphthalenes
;
chemistry
;
isolation & purification
;
Sesquiterpenes
7.Preparation of baicalin-chitooligosaccharide compound and its characterization.
Yan-Rong JIANG ; Zhen-Hai ZHANG ; Yu-Miao YE ; Hong-Mei YAN ; Dong-Mei DING ; Xiao-Bin JIA
China Journal of Chinese Materia Medica 2014;39(4):648-652
To apply chitooligosaccharide in the preparation of baicalin compound, in order to increase the drug dissolution in vitro, and investigate the basic property of the compound. Baicalin-chitooligosaccharide compound was prepared by using the solvent method. The structure and physicochemical properties of compound were analyzed by using differential scanning calorimetry (DSC), scanning electron microscopy (SEM), X-ray powder diffraction (XRD) and infrared vibrational spectrum (IR), and its dissolution behavior was also investigated. The results showed that the compound prepared at baicalin-chitooligosaccharide molar ratio of 1 : 1 could significantly improve the dissolution of baicalin. The results of DSC and XRD analysis suggested that baicalin may exist in an amorphous state. IR results indicated the interaction between baicalin and chitooligosaccharide. The baicalin-chitooligosaccharide compound could significantly improve dissolution in vitro of drug.
Calorimetry, Differential Scanning
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Chemistry, Pharmaceutical
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Drug Carriers
;
chemistry
;
Drugs, Chinese Herbal
;
chemistry
;
Flavonoids
;
chemistry
;
Oligosaccharides
;
chemistry
;
Spectroscopy, Fourier Transform Infrared
8.NMR-based analysis of water soluble extracts of different Astragali Radix.
Dong TIAN ; Zhen-Yu LI ; Sheng-Ci FAN ; Jin-Ping JIA ; Xue-Mei QIN
Acta Pharmaceutica Sinica 2014;49(1):89-94
Water soluble extract (WSE) is an important index for the quality evaluation of Astragali Radix (AR). In this study, the WSE of the wild AR from Shanxi province (SX) and the cultivated AR from Gansu Province (GS) were compared. The WSEs of two types of AR were determined according to the appendix of Chinese pharmacopoeia. Then the WSEs were subjected to NMR analysis, and the obtained data were analyzed using HCA, PCA, OPLS-DA, microarray analysis, and Spearman rank analysis. In addition, the Pearson correlation of differential metabolites were also calculated. The results showed that the WSE content of GS-AR (37.80%) was higher than that of SX-AR (32.13%). The main constituent of WSE was sucrose, and other 18 compounds, including amino acids, organic acids, were also detected. Multivariate analysis revealed that SX-AR contained more choline, succinic acid, citric acid, glutamate, taurine and aspartate, while GS samples contained more sucrose, arginine and fumaric acid. In addition, the Pearson correlations between different metabolites of the two types of AR also showed apparent differences. The results suggested that the WSE of two types of AR differs not only in the content, but also in the chemical compositions. Thus, the cultivation way is important to the quality of AR. This study supplied a new method for the comparison of extract of herbal drugs.
Arginine
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analysis
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Aspartic Acid
;
analysis
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Choline
;
analysis
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Citric Acid
;
analysis
;
Drugs, Chinese Herbal
;
analysis
;
chemistry
;
Fumarates
;
analysis
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Glutamic Acid
;
analysis
;
Magnetic Resonance Spectroscopy
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Multivariate Analysis
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Phylogeography
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Plant Roots
;
chemistry
;
Plants, Medicinal
;
chemistry
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Succinic Acid
;
analysis
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Sucrose
;
analysis
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Taurine
;
analysis
9.Angiomyolipoma of the kidney with lymph node involvement.
Chuan-Zhen WU ; Feng-Hua WANG ; Cheng-Mei LI ; Wen-Chang FANG ; Jia-Ni YU
Chinese Journal of Pathology 2005;34(11):715-715
Adrenalectomy
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Adult
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Angiomyolipoma
;
pathology
;
surgery
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Follow-Up Studies
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Humans
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Kidney
;
pathology
;
Kidney Neoplasms
;
pathology
;
surgery
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Lymph Node Excision
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Lymph Nodes
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pathology
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Lymphatic Metastasis
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Male
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Nephrectomy
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Ureter
;
surgery
10.Analysis on tear film after LASIK by femtosecond laser with Oculus corneal topography
Yuan, ZHANG ; Bing-Bing, JIA ; Yan, ZHANG ; Dong-Mei, GAO ; Yu-Zhen, PANG
International Eye Science 2014;(6):1116-1118
AIM:To observe the changes of tear film on the patients after laser in situ keratomileusis ( LASIK) with corneal flap created by femtosecond laser with Oculus corneal topography.
METHODS:Totally 120 myopic patients (240 eyes) were collected who underwent femtosecond laser surgery LASIK from August to September 2013, and these patients can be followed up for 3mo. Tear break-up time ( BUT) and tear meniscus height ( TMH ) with Oculus corneal topography were recorded preoperatively and postoperatively at 1wk;1, 2 and 3mo.
RESULTS: Oculus BUT: there existed obvious differences (P=0. 012, 0. 000, 0. 023<0. 05) in 1wk, 1 and 2mo compared with the preoperative level. While no such obvious difference ( P = 0. 236 > 0. 05 ) existed in 3mo compared with the preoperative level. TMH:there existed obvious differences (P=0. 025, 0. 019, 0. 026<0. 05) in 1wk, 1 and 2mo compared with the preoperative level. No such obvious difference ( P = 0. 375>0. 05 ) existed in 3mo compared with the preoperative level.
CONCLUSION: Femtosecond laser surgery affects the stability of the tear film at a certain time and a certain extent. The mechanism related to many factors. It is temporary and lighted.