Objective:To investigate influences of bicortical anchora ge on the values of natural frequency of dental implants. Methods: Using the commercial code of SolidWorks, 3-D models of a screw-shaped dental i mplant and a mandibular bone segment were generated. After the 3-D implant-bon e complex was meshed by ABAQUS software, effects of bicortical anchorage on the buccolingual and axial first-order natural frequencies of the implant were comp uted. Results:Bicortical anchorage increased the buccolingual na tural frequencies and the axial natural frequencies of the implants by a maximu m of 13.77% and 107.40% respectively. The apical anchorage depth in the cortical bone had an insignificant effect on further increase of implant stability. Conclusion:Bicortical anchorage can increase the stability of dental implants, especially in the axial direction.

Objective To elucidate the relationship between Child-Pugh grading and the degree of hep- atic cirrhosis.Method Six patients each with hepatic cirrhosis in Child-Pugh grade A and B were selected.The livers were observed with gross examinations during operation,and the liver specimens were observed with gross ex- aminations and also checked with light and electron microscopies.Results With naked eyes,it was quite difficult to find differences in size,shape and between different Child-Pugh grades.With hght microscopy,hepatic fibrosis of Ⅰ-Ⅱ degrees was commomly seen in Child-Pugh grade A and necrosis of hepatic cells was slight,while fibrosis of Ⅲ-Ⅴ degrees was usually found in grade B and necrosis was severe.With electron microscopy the collagen fib- rils in Disse's spaces were white cloud-like in grade A,while they were band-like in grade B.Of swelling and de- generation of organellae of hepatic and Kupffer cells,they were worse in grade B than in grade A.Conclusion The parameters in Child-Pugh grading are not complex,but they can be used to evaluate the degree of lesions. There is a positive relationship between them.

Ovbective To evaluate whether the deleterious effect of cardiopulmonary bypass (CPB) mediated by oxygen free radicals could be prevented by controlling PaO 2 in cyanotic children. Methods Cyanotic pediatric patients undergoing cardiac surgery (n=38) were randomly allocated into 2 groups. The groups matched in respect to sex,age,body surface area and preoperative ventricular function. Group H (n=18) had CPB initiated at a FiO 2 of 0.8,group L (n=18) had CPB initiated at a FiO 2 of 0.4. SOD and MDA were determined in arterial blood sample before CPB,30 minutes after initiation of CPB and 30 minutes post CPB. Various pulmonary parameters were determined 10 minutes after intubation,30 minutes after CPB,the 2nd hours and the 6th hours in the intensive care units. Results At the 6th hours in ICU,the mean postoperative increase in peak airway pressure and P(A-a)O 2/PaO 2 in group H was significant higher than that in group L(P

Objective To improve the diagnostic accuracy of nonpalpable calcified breast lesions by establishing a Logistic multivariate prediction model to assess the probability of benign/malignant breast lesions.The proposed model is based on the clinical and BI-RADS-X-ray imaging features of patients with nonpalpable calcified breast lesions.Methods A total of 147 nonpalpable calcified breast lesions were analyzed retrospectively.Firstly, based on the personal experience,the X-ray imaging data of lesions were analyzed to obtain the BI-RADS categorization, and the ROC curve was plotted by comparison with pathology.Then the univariate and multivariate analysis was performed on the clinical and X-ray imaging features of pathology to select the independent factors related to benign/malignant features.Further,a Logistic regression model was built,the suitable cut-off point was determined, and the ROC curve was obtained.Finally,the comparisons of the diagnostic accuracy of breast lesions were made between the method using the BI-RADS categorization and the method using the Logistic regression model.Results The AUC of the BI-RADS method was 0.867 9.The univariate analysis showed that there exist statistical differences among clinical features of patients(age,location,and quadrant),as well as the BI-RADS-X-ray imaging features (distribution,morphological and gland density).Also,by using the multivariate Logistic regression equation,the statistical differences among age,quadrant and morphological difference can be observed.The AUC using the built Logistic regression model was 0.906 3.Conclusion The diagnostic accuracy of breast lesions using the Logistic model is higher than that using the BI-RADS categorization method.Therefore, the proposed model is valuable for obtaining accurate diagnosis of breast lesions.

Acupuncture Therapy ; Aged ; Female ; Humans ; Male ; Middle Aged ; Pneumoconiosis ; complications ; therapy ; Pulmonary Disease, Chronic Obstructive ; etiology ; therapy ; Treatment Outcome

Objective To determine whether tChoI and ADC and their changes could be applied to predict pathologic response be-fore,during and after NAC of locally advanced breast.To assess the diagnostic performance of each parameter.Methods The tChoI and ADC and their changes in 22 women who underwent MRI before,during and after the NAC were analyzed prospectively.All pa-tients were divided into major histological response group and non-major histological response group by methods of Miller and Payne system according to the final pathologic response.Pre-and post-treatment measurements and changes in tChoI and ADC values in MHR versus NMHR were analyzed using Mann-Whitney U test.ROC curve analysis was performed to assess the diagnostic per-formance of each parameter and also to identify which parameter could be used to predict the pathologic response to NAC and find the optimal cut off value for MHR prediction.Results After NAC,14 patients showed MHR and 8 showed NMHR.After NAC,all the parameters and their changes were significantly different between the MHR and NMHR groups,the change rate of all parameters af-ter NAC can actively diagnosis the MHR according ROC(AUC>0.5).Using 0.913 ×10 -3 mm2/s of ADCmin after NAC as the cut off value,prediction of MHR with sensitivity and specificity was 85.7% and 100%,respectively.Using 81.25% of the change rate of tCho I after NAC as the cut off value,prediction of MHR with sensitivity and specificity was 71.4% and 100%,respectively(AUC=0.881,P =0.008).The △ADCmin 2% and △tChoI2% are better than △ADCmean 2%.Conclusion Application of 3D 1 H-MRS and DWI can predict pathologic response and may provide more detailed and accurate evidence for subsequent treatment.

Objective To analyze the correlation between the expression of Abl interactor 1 (ABI1) and the clinicopathologic characteristics in colorectal adenocarcinoma tissues.Methods Immunohistochemistry was used to determine ABI1 expression in human colorectal adenocarcinoma tissues and matched adjacent tissues.A statistical analysis was used to determine the potential correlation between ABI1 expression and clinicopathological characteristics and prognosis.Results ABI1 is up-regulated in colorectal adenocarcinoma tissues compared with matched adjacent tissues (P =0.000),ABI1 expression is significantly correlated with infiltration (P =0.043) and differentiation (P =0.040),but not with sex,age,tumor location,clinical stage,lymph node metastasis and distant metastasis (P ＞0.05);It was also shown that ABI1 expression had a significant influence on prognosis (x2 =11.090,P =0.001).Multivariate analyses showed that high ABI1 expression is not an independent poor prognostic factor for overall survival (P =0.119).Conclusion ABI1 overexpression might act as pro-oncogene for patients with colorectal adenocarcinoma.

Drug Therapy, Combination ; Drugs, Chinese Herbal ; therapeutic use ; Humans ; Medicine, Chinese Traditional ; Phytotherapy ; Rheumatic Diseases ; diagnosis ; drug therapy

Ribonucleic acid (RNA) medicines have strong therapeutic potential for numerous rare genetic illnesses and malignancies because of its exact programmability based on Watson-Crick base pairing principle and unique ability to regulate gene expression. However, RNA medicines still have limitations in many areas, including stability, half-life time, immunogenicity, organ selectivity, cellular uptake and endosomal escape efficiency despite their great therapeutic potentials. This review briefly introduced numerous RNA medications [mostly messenger RNA (mRNA), small interfering RNA (siRNA), microRNA (miRNA) and antisense oligonucleotide (ASO)] that have intrigued of researchers in recent years, as well as their action mechanism in vivo. A number of delivery techniques, such as chemical modification, ligands coupling and nanocarriers have been proposed. The manufacture and applications of lipid nanoparticle, polymer nanoparticle and exosomes were discussed in depth. The goal of this work is to give a theoretical foundation and design concepts for the development of effective and safe RNA delivery technology, as well as to facilitate RNA therapeutic clinical translation.

ObjectiveTo explore the effects of fluoxetine on special learning and memory and serum S100B level in depressed model rats.MethodsAdult male SD rats were divided into six groups randomly according random digits table:control group ( A ),depressed model group ( B ),group of depressed model treated with single dose of fluoxetine for one day ( C ),group of depressed model treated with fluoxetine for one week (D),group of depressed model treated with fluoxetine for two weeks (E) and group of depressed model treated with fluoxetine for four weeks (F),ten rats in each group.Except control group,others were subjected to forced-swimming for four weeks,15 min a day.Fluoxetine (10 mg/kg) was given intragastric administration to group C-F before swimming everyday.Morris water maze ( MWM ) was used to measure the spatial learning and memory of rats.ELISA was used to determine the level of serum S100B.ResultsIn the hiding platform test of MWM,there was significant longer of escape latency (EL) in B group than that in A group(P ＜ 0.05 ).And the EL in all groups treated with fluoxetine became shorter with the prolonging of treatment.In the probe test,there were significant longer time in target quadrant in D,E,F than in other quadrant (F =5.162,P ＜ 0.01 ).The levels of serum S100B were lower in E,F groups ( E group ( 0.91 ± 0.23 ) ng/ml,F group ( 0.85 ± 0.21 ) ng/ml) than that in B group (( 1.26 ±0.61 )ng/ml,P＜0.05).ConclusionChronic administration of fluoxetine could improve the impairment of spatial learning and memory and reverse the increase of S100B level in serum of depressed model rats.