Adult ; Causality ; Death, Sudden ; epidemiology ; Female ; Herbicides ; poisoning ; Humans ; Male ; Middle Aged ; Paraquat ; poisoning ; Retrospective Studies ; Young Adult

OBJECTIVE:To investigate the influence and safety of etomidate or sevoflurane combined with sacral or epidural block anesthesia on anesthesia effects and inflammatory factors. METHODS:160 children undergoing surgery selected from our hospital during Feb. 2012 to Dec. 2015 were divided into group A and B according to random number table,with 80 cases in each group. Group A was given etomidate 3 mg/(kg·h)for anesthesia maintenance+sacral or epidural block;group B received sevoflu-rane inhalation 1%-3% for anesthesia maintenance+sacral or epidural block. The anesthesia effects were compared between 2 groups as well as the levels of serum S100β,NSE,Aβ and inflammatory factors before and after surgery. The occurrence of ADR was observed. RESULTS:There was no statistical significance in SpO2 between 2 groups before and after surgery(P>0.05). The anesthesia induction time,postoperative recovery time and heart rate of group A were significantly longer or higher than those of group B,with statistical significance(P<0.05). There was no statistical significance in the levels of serum S100β,NSE,Aβ and inflammatory factors between 2 groups before surgery(P>0.05). After surgery,the levels of serum S100β,NSE,Aβ,CRP,IL-2 and IL-6 were significantly increased in 2 groups,and group A was significantly higher than group B,with statistical significance (P<0.05);there was no statistical significance in TNF-α before and after surgery or between 2 groups (P>0.05). No obvious ADR was found in 2 groups. CONCLUSIONS:Sevoflurane combined with sacral or epidural block anesthesia is better than etomi-date combin ation anesthesia,with in mild inflammatory reaction and good safety.

Objective:To observe the color change of tooth i nduced by hydrogen peroxide gel. Method:12 extracted teeth were randomly assigned to two groups (n=6) and treated with 350 g/L and 75 g/L hy drogen peroxide gel respectively. One of the 6 samples in each group was used as the control. The bleaching gel was placed onto the enamel surface of the sample s. In 350 g/L gel group digital photographs were taken just before,10 and 30 min after the process, in 75 g/L gel group digital photographs were taken before an d 1.5?N(N=0,1,2,3,4,5,6,7) hours after the process respectively. All photograph s were taken at the same illuminating conditions and photographic parameters wer e analyzed with Photoshop7.0 software. Results:10.5 h after trea tment in the 75 g/L gel group L *, a * and b * values on the teeth surface were in creased(+) or decreased(-) by +4.5,-1.4 and -0.5,those on the inner sections 2.7 ,-1.0 and -0.8,respectively. 30 min after treatment in the 350 g/L gel group L *, a * and b * values on the teeth surfaces were increased(+) or decreased (-) by +1.9,-0.6 and 0,those on the inner sections +0.4,-0.4 and -0.6,respectively. Conclusion:Effective bleaching effect may be obtained in 10~30 min b y 75 g/L or 350 g/L hydrogen peroxide gel on teeth surface and in inner dentin. 75 g/L hydrogen peroxide bleaching gel may produce more reliable and save effect than 350 g/L.

Objective To observe the expression levels of IL-8,IL-4,TNF-α of neonatal mouse with systemic inflammatory response syndrome(SIRS) treated by different doses of dexamethasone.Methods A total of 50 neonatal mice were randomly divided into five groups:blank control group,saline control group and treatment group A,B,C.The saline control group and treatment groups were established into SIRS models,treatment group A:a single high-dose of dexamethasone (2 mg/kg),subcutaneous injection (SC) ; treatment group B:two doses of dexamethasone (1 mg/kg,q1 2 h,total 2 mg/kg,SC) ; treatment group C:four doses of dexamethasone (0.5 mg/kg,q 1 2 h,total 2 mg/kg,SC) ;saline control group:saline of the same volume (0.4 ml/kg,SC).All mice were detected IL-4,IL-8,TNF-α by ELISA 72 hours after animal models being completed.Results The levels of IL-4,IL-8,TNF-α in saline control group and treatment group A,B,C were higher than those in blank control group respectively (P ＜ 0.05,respectively).The levels of IL-4,IL-8,TNF-α in treatment group A,B,C were lower compared to those of saline control group respectively (P ＜ 0.05,respectively),levels of TNF-α,IL-8 in treatment group B and C were lower than those of treatment group A(P ＜ 0.05,respectively).There were no significant differences in the level of IL-4 among treatment group A,B,and C (P ＞ 0.05).Conclusion Dexamethasone could lower the expressions of pro-inflammatory cytokines IL-8,TNF-α and anti-inflammatory factor IL-8 of neonatal mouse with SIRS,and the effect of multiple low-doses of dexamethasone on SIRS is significantly better than a single high dose.

Objective To investigate the relationship between vascular injury of diabetic rats with hind limb ischemia and endothelial nitric oxide synthase(eNOS) uncoupling.Methods 15 SD rats were randomly divided into 3 groups:control group (n =5),diabetes group (n =5) and diabetes ischemia group (n =5).Two weeks after the diabetic rat model and hind limb ischemia of diabetic rat model were set up,Enzyme linked immunosorbent assay(ELISA) was used to detect nitric oxide(NO) expression in the rat femoral artery; Dihydroethidium(DHE) fluorescent probe was used to detect superoxide(O2-) production in the frozen section of femoral artery; and Western blot was used to examine GTP cyclohydrolase 1 (GTPCH-1)expression in femoral artery.Results ①Compared with the control group,NO expression of femoral artery in diabetes group was significantly reduced,diabetes ischemia group decreased more significantly,and the difference between the 3 groups had statistical significance(P =0.000,P =0.000,P =0.001).②Frozen section showed:compared with the control group,O2-expression of diabetes group significantly increased,but not as much as diabetes hind limb ischemia group.③Western blot results showed that compared with the control group,the expression of GTPCH-1 in diabetes group reduced,diabetes hind limb ischemia group decreased more significantly,and the difference between the 3 groups had statistical significance (P =0.001,P =0.000,P =0.012).Conclusion eNOS uncoupling is present in diabetic rats with hind limb ischemia and vascular injury.

Objective:To comparatively observe the effects of 20(R)-ginsenoside Rg3 and PEG-PLGA-Rg3 nanoparticles on the Lewis lung cancer mice and to explore the mechanisms of Rg3 and PEG-PLGA-Rg3 nanoparticle anti-cancer in vivo.Methods:Lewis lung cancer mouse model was established and 60 mice were randomly divided into 5 groups with twelve in each group:PEG-PLGA-Rg3 nanoparticles group (Rg3-N),PEG-PLGA group (PEG),Rg3 group (Rg3 ),normal control group (C),saline control group(NS),and received intragastric administration for 1 4 days.The weights of the mice were measured every 2 days and the weight curves were obtained.At the same time,the color pattern,activity and men-tal status were observed.The mice were sacrificed when the administration was over,and the effects of 20 (R)-ginsenoside Rg3 and PEG-PLGA-Rg3 nanoparticles on tumor weight,and the tumor:weight ratios were analysed.In addition,the tumor microvessel density (MVD)was measured by immunohistochemi-cal staining with anti-CD31 antibody to compare the effects of Rg3 and PEG-PLGA-Rg3 nanoparticles on the tumor angiogenesis in vivo.Furthermore,the levels of such angiogenesis and proliferation factors as MMP-9,HIF-1 α,VEGF,Ki-67 were examined by RT-PCR,Western blot and immunohistochemistry to explore the internal molecular mechanisms of anti-tumor effects in vivo.Results:The trends of variation of the mice weights in NS group and PEG group were rising early but declining later.In contrast,the trends of the other three groups were rising early and became stable later.In comparison with NS group, the mice of Rg3 group and Rg3-N group had better general status:brighter color,more active and better spirit.Compared with NS group,the tumor weight in PEG group,Rg3 group and Rg3-N group showed no significant difference but the tumor:weight ratio and MVD in Rg3 group and Rg3-N group declined signi-ficantly (P <0.01 ).Besides,there was no significant difference between Rg3 group and Rg3-N group. At the same time,the level of VEGF mRNA,the protein expression of MMP-9,HIF-1 α,VEGF in Rg3 group and Rg3-N group decreased compared with NS group.Furthermore,the level of each index above-mentioned in Rg3-N group was lower than that in Rg3 group.The expression of Ki-67 in PEG group,Rg3 group and Rg3-N group showed no significant difference compared with NS group.Conclusion:Rg3 and PEG-PLGA-Rg3 nanoparticle may suppress the expression of VEGF,MMP-9 and HIF-1 αin Lewis lung cancer mice,thereby indirectly contributing to their antitumor effects and alleviating the mice’s general status.In addition,PEG-PLGA nanoparticles embedding can promote Rg3 antitumor effect in vivo.

Coronary Angiography ; adverse effects ; Heart Atria ; Humans ; Male ; Middle Aged

Objective To investigate the correlation between iodine intake,BRAF mutation in thyroid gland and clinical biologic characteristics in papillary thyroid carcinoma(PTC) cases.Methods A total of 159 PTC patients and 200 healthy controls were enrolled in this study.Urine iodine was tested,BRAFV600E mutation was detected by PCR.The correlation was analyzed between BRAF mutation and iodine intake,BRAF mutation and clinical biologic characteristics of PTC respectively.Results The median urinary iodine (MUI) of the patients and healthy controls was 336 μg/L and 196 μg/L respectively (P =0.004).The overall prevalence of the BRAFV600E mutation in this series of PTC was 63.5％,showing a clear correlation of BRAFV~E mutation with iodine intake (P =0.006).There was no correlation of BRAFV600E mutation with age,gender,tumor size,extrathyroid extension or nodulor goiter in PTC (P ＞ 0.05).But there was a significant association of BRAFV~E mutation with lymph node metastasis (P =0.008) and Hashimoto's thyroiditis (P =0.037).Conclusions High iodine intake may be a risk factor for PTC occurrence.In PTC cases,high iodine intake may be a risk factor of BRAFV600E mutation.BRAFV600E mutations increase both in PTC with cervical lymph node metastases and uncoexisting Hashimoto' s thyroiditis.

Objective To develop a scientific system of core information of health and evaluation index for the elderly Chinese people,which is suitable for the situation of our country.Methods The framework was established by extensive literature review and topic discussions.The index system was constructed with the Delphi method.Results 58 experts in different fields of health were selected in the two rounds of Delphi consultation research.The effective response rates in the two rounds were 96.88％ and 100％ respectively.The average value of the authoritative coefficient was between 0.80 and 0.96.And coefficients of variation were between 6.20 and 12.13.Based on the Delphi consultation research,the core information of health and evaluation index system for the elderly Chinese people were composed of 8 primary indicators closely associated with the positive awareness of aging and senescence and 31 other indicators.Conclusions The architecture of the core information of health and evaluation index system for the elderly Chinese people is stable,and the consultant experts tend to reach a consensus.The evaluation index system contains the most influential factors for the elderly Chinese people,and has the affirmative scientificity,applicability and feasibility.

Holistic education is an important philosophy of medical education.Medical graduate students can show their skills of medical specialties,improve their abilities of communication and cooperation,and promote their sense of social responsibility through social practice.Through conducting questionnaire among medical graduate students of Peking University Third Hospital who major in internal medicine,we have got to know the effect of social practice on holistic education philosophy.Meanwhile,social practice is a crucial approach to implementing holistic education.