Objective To investigate the expression of phosphatidylinositol 3-kinase(PI-3K)in adipose tissue of polycystic ovary syndrome patients(PCOS),and explore molecular mechanisms of insulin resistance(IR)in PCOS.Methods Samples from patients with PCOS with IR(n=19),PCOS without IR (n=10)and controls(n=15)were collected.Serum fasting insulin(FIN)and fasting plasma glucose (FPG)were measure& Insulin resistance index was calculated using homeostasis model assessment (HOMA)to analyze the relationship between these markers and IR.Western blot technique was used to detect the PI-3K p85 subunit.Gene expression of PI-3K p85 subunit was detected by reverse transcription polymerase chain reaction(RT-PCR)method.Kinase activity was detected by immunoprecipitation,thin- layer chromatography and gamma scintillation counting.Results(1)The levels of FIN[(25.2?3.8) mU/L]and HOMA-IR(1.6?0.3)in PCOS with IR were significantly higher than those in PCOS without IR[(13.4 +3.8)mU/L,0.9?0.3]and controls[(9.5 +2.6)mU/L,0.5?0.3;all P＜0.05).(2) There was no significant difference in the protein(0.65?0.10)and gene expression(0.92?0.12)of PI-3 K p85 subunit in PCOS with IR compared with PCOS without IR(0.72?0.10,1.01?0.10)and control groups(0.73?0.14,1.00?0.12;P＞0.05).(3)PI-3K activity in PCOS with IR(81%)and PCOS without IR(89%)was significantly decreased(P＜0.01,P＜0.05)and negatively correlated with HOMA- IR(r=-0.69,P＜0.01;r=-0.62,P＜0.05).Conclusions No significant difference in the protein and gene expression of PI-3K p85 subunit in PCOS with IR is found.The decreased PI-3K activity may lead to IR of PCOS.

OBJECTIVETo study the effect of chronic occupational stress on the glucocorticoid receptor (GR) and immune function.

METHODSA cross-sectional study was conducted in 112 railway station dispatchers. Perceived job stress was assessed by means of the Chinese version of the Job Content Questionnaire. The subjects were divided into high, medium and low strain groups according to the job strain score of the questionnaire. The number of GR, percentage of T lymphocyte subpopulations, concentrations of cortisol and interleukin 2 (IL-2) in blood were measured.

RESULTSThe concentrations of serum cortisol in high and medium strain groups [(295.43 +/- 79.06) and (274.34 +/- 70.08) ng/ml respectively] were higher than that in low strain group [(181.01 +/- 53.41) ng/ml, P < 0.05]. GR binding capacity in both groups (4,330.0 +/- 1,001.0, 3,971.6 +/- 966.8 specific binding/cell respectively) were smaller than that in low strain group (5,141.3 +/- 1,068.5 specific binding/cell, P < 0.05). The percentage of CD(3) T lymphocyte in high strain group was higher than that in low strain group [(50.21 +/- 10.30)% vs (56.87 +/- 15.36)%, P < 0.05], and CD(4) T lymphocyte in high and medium strain groups were significantly smaller than that in low strain group [(23.27 +/- 10.01)%, (27.06 +/- 7.47)% vs (33.31 +/- 7.77)%, P < 0.05]. In contrast, the percentage of CD(8) T lymphocytes in high and medium strain groups were significantly higher than that in low strain group [(28.16 +/- 6.47)%, (25.54 +/- 6.70)% vs (21.91 +/- 5.93)%, P < 0.05]. The levels of serum IL-2 in high and medium groups were smaller than that in low strain group [(0.77 +/- 0.05), (0.80 +/- 0.07) vs (1.05 +/- 0.12) ng/ml, P < 0.05]. Correlation analysis showed that serum cortisol level was negatively correlated with CD(8) percentage (r = -0.612, P < 0.01).

CONCLUSIONChronic occupational stress may induce rise of glucocorticoid, down-regulation of GR and inhibition on immune function.

CD4-CD8 Ratio ; Cross-Sectional Studies ; Female ; Humans ; Hydrocortisone ; blood ; Male ; Occupational Diseases ; immunology ; metabolism ; Receptors, Glucocorticoid ; analysis ; Stress, Psychological ; immunology ; metabolism

Objective To explore the prevalence of intolerance to eggs and its relationship with body mass index in Beijing,China.Methods A cross sectional survey which included 12 766 adults from health sciences center of general hospital of the Chinese People's Liberation Army from August,2008 to July,2009 was carried out.Data was entered computer and organized by EpiData 3.0 software for epidemiological analysis.Nonconditional logistic regression model was used for odd ratio (OR) and 95％CIcalculation,and statistics analysis was carried out by SPSS 13.0 software.Results Positive rate of intolerance to eggs in the cohort was 28.5％.Grades of intolerance to eggs were associated with sex(P＜0.05 ).Positive rate of intolerance to eggs in females(36.5％ ) was higher than those in males (24.9％).Data from multiple regression analysis showed that intolerance to eggs was associated with sex and body mass index.Compared with males,the OR(95％CI) of female was 1.732 (1.590-1.887).Compared with normal weights,the OR (95％ CI) of low weights was 1.443 ( 1.018-2.045 ).Conclusion Sex and body mass index might be associated with intolerance to eggs.

Objective To study local and systemic immune response in an animal model treated with recombinant hIFN-α-2b-BCG instillation. Methods The MB49 orthotopic bladder cancer model in C57BL/6 mice was established and treated separately with rBCG, wild BCG, wild BCG combined with IFN-α-2b and PBS as the control. The changes of lymphocyte subgroups in peripheral blood were analyzed with FCM, and mTNF-α and mIL-12 in peripheral blood of mice were detected with ELISA.Immunohistochemistry was carried out to detect the local immune reaction, T cell subsets and FAS, in bladder cancer after being treated with rBCG or wBCG. Results The content of CD4+ T lymphocyte was up-regulated in the rBCG group. The CD4+/CD8+ ratio of 2. 63 was up-regulated than pretreatment, significantly different than that of wBCG group(P＜0.05). ELISA assay showed that BCG significantly up-regulated the level of mTNF-α and mIL-12 in serum of orthotopie murine bladder cancer mice. The mTNF-α 806 pg/ml, mIL-12 860 pg/ml in rBCG group, was not significantly higher than those in wBCG group and combination group. The immunocompetent cell numbers with CD3, CD4,CD8 phenotype increased significantly in the tumor tissue of BCG treated group than the control(P＜0.05). The results of CD4+ in rBCG group and the combination group, and CD8+ in rBCG group were significantly higher than that of the wBCG(P＜0.05). The expression of Fas in tumor tissues treated with intravesical BCG was increased(P＜0. 05). Conclusions The recombinant IFN-α-2b-BCG can retrieve the disproportion of systemic lymphocyte subgroups, and increases Th1-type factors and local Fas expression in orthotopic murine bladder cancer. The recombinant IFN-α-2b-BCG is effective in regulating local and systemic immune reaction in orthotopic murine bladder cancer model.

OBJECTIVETo explore gender difference of relationship between occupational stress and depressive symptoms.

METHODSThe cross-sectional study was used to investigate 5338 workers in 13 factories and companies, the depressive symptoms were assessed with Epidemiologic Studies Depression Scale (CES-D) and the perceived occupational stress was evaluated by the Chinese version of the Job Content Questionnaire (JCQ) and Effort-Reward Imbalance (ERI) Model Questionnaire. Logistic regression analyses were conducted to estimate the associations between occupational stress and prevalence of depressive symptoms.

RESULTSPrevalence of depressive symptoms was 31.8% for all subjects, 33.8% for males and 27.7% for females, gender difference of prevalence was significant (P < 0.01). Psychological demands, physical demands, job control, effort, overcommitment and negative affectivity scores for males were significantly higher than those for females(P < 0.01 or 0.05), social support, reward and job satisfaction scores for males were significantly lower than those for females (P < 0.01). Psychological demands, physical demands, job control, effort, overcommitment and negative affectivity scores for workers with depressive symptoms were significantly higher than those for workers without depressive symptoms (P < 0.01) but job control, social support, reward, job satisfaction and positive affectivity scores for workers with depressive symptoms were significantly lower than those for without depressive symptoms (P < 0.01). Psychological demands, physical demands, job control, effort, overcommitment and negative affectivity scores for male workers with depressive symptoms were significantly higher than those for female counterparts (P < 0.01), but social support, reward, job satisfaction scores for male workers with depressive symptoms were significantly lower than hose for female workers with depressive symptoms (P < 0.01). Ratio of male workers and male workers with depressive symptoms with job strain and ERI > 1, high demands and low control, and high effort and low reward were higher than female counterparts (P < 0.01). The risk of depressive symptoms for female with high demands and low control was about two times as high as that for female workers with low demands and high control for psychological demands and physical demands (OR = 2.0), risk for females was slightly higher than that for males (OR = 2.04 and 2.17, respectively). The risk of depressive symptoms for workers with high efforts and low rewards was about two times as high as that for workers with low efforts and high rewards (OR = 1.70). No interactive effect between gender and job strain and ERI on depressive symptoms were found.

CONCLUSIONThe gender difference of relationship between depressive symptom prevalence and job strain may due to the difference of psychosocial factors between females and males. There may be no interactive effect between psychosocial factors and gender on depressive symptoms.

Adolescent ; Adult ; Cross-Sectional Studies ; Depression ; epidemiology ; Female ; Humans ; Job Satisfaction ; Male ; Middle Aged ; Sex Factors ; Stress, Psychological ; epidemiology ; Surveys and Questionnaires ; Young Adult

Objective To knockout the MATP gene of mouse melanoma cell line B16F10 using CRISPR/Cas9 system,and to lay foundation for the functional study of MATP gene.Methods Specific primers of MATP were designed according to the report in http://crispr.mit.edu/ website.The primers were linked to pCAS9/gRNA1 vector.Then the positive vector was transfected into mouse melanoma B16F10 cells,and monoclonal cell lines were obtained by the infinite dilution method.After the genomes of different monoclonal cell lines were extracted and sequenced,the cell lines with MATP gene cleavage were screened,and the expression of MATP in these cell lines was verified by Western-blot analysis.Results Three MATP gene knockout cell lines were successfully obtained.The western-blot results showed that the cell lines did not express MATP protein.Conclusions The knockout of MATP gene in B16F10 cell line can be successfully achieved using the pCAS9/gRNA1 vector.

OBJECTIVETo evaluate the value of rectangle titanium cage (SynCage-C) in the anterior cervical spine fusion.

METHODSNineteen patients underwent anterior cervical discectomy and interbody fusion using SynCage-C with average follow-up of 9 months.

RESULTSAll cases had cervical spondylosis with 13 cases of myelopathy and 6 cases of radiculopathy. One level fusion were 16 cases and two levels were 3 cases. The mean operation time was 40 minutes per level with average bleeding 60 ml. Patients were asked to wear a hard collar for average 8.5 weeks after the operation. The fusion was solid on lateral view X-ray films three months post-operation. Only 1 case had neck stiffness due to prolonged collar protection and another case had short term pain on bone dorner site. There were no other complications such as cage subsidence, displacement, milaligment and kyphosis of cervical spine.

CONCLUSIONThe SynCage-C can resort and maintain the intervertebral space effectively with the advantage of immediate stability, satisfactory fusion and easy to use.

Adult ; Aged ; Cervical Vertebrae ; surgery ; Equipment and Supplies ; Female ; Follow-Up Studies ; Humans ; Male ; Middle Aged ; Orthopedic Procedures ; instrumentation ; methods ; Spinal Diseases ; surgery ; Spinal Fusion ; methods ; Titanium ; therapeutic use ; Treatment Outcome

OBJECTIVETo explore the relationship between occupational stress and musculoskeletal disorders in lower extremity.

METHODSThe cross-sectional study was used to investigate 5338 workers in 13 factories and companies for prevalence of musculoskeletal disorders in lower extremity, working postures and occupational stress were investigated with questionnaires for past year. Perceived occupational stress was evaluated by the Chinese version of the Job Content Questionnaire (JCQ) and Effort-Reward Imbalance (ERI) Model Questionnaire. Logistic regression analyses were conducted to estimate the associations between occupational stress and prevalence of musculoskeletal disorders in lower extremity.

RESULTSIn the main dimensions of job strain and ERI model, physical demands and effort were risk factors of hip disorders, but job control was a protective factor of hip disorders. When job strain and ERI model scales were analyzed simultaneously, only physical demands were risk factor of hip disorders. When job strain and the effort-reward imbalance were computed by creating four independent categories, job strain was a risk factor of hip disorders, ERI was a risk factor of hip and knee disorders. The risk of imbalance between job strain and effort-reward the protective effect of job control increased with the disorder regions. According to continuous variable analysis, job strain by calculated with physical demands was risk factor of hip disorders, continuous ERI variable was risk factor of hip and knee disorders.

CONCLUSIONOccupational stress is associated significantly with musculoskeletal disorders in lower extremity. The control of occupational stress and increase of job control should be considered for preventing the musculoskeletal disorders in lower extremity.

Adult ; Cross-Sectional Studies ; Female ; Humans ; Job Satisfaction ; Lower Extremity ; physiopathology ; Male ; Musculoskeletal Diseases ; epidemiology ; Occupational Diseases ; epidemiology ; Risk Factors ; Stress, Psychological ; epidemiology ; Surveys and Questionnaires

OBJECTIVETo analyze the distribution of the musculoskeletal disorders, work load and working postures in different factories, gender, education levels, age and working years among manufacturing workers.

METHODSIn a cross-sectional study of 5134 manufacturing workers in 12 factories, the morbidities for musculoskeletal disorders in one year period were measured with questionnaires.

RESULTSThe morbidities for musculoskeletal disorders in body sites: waist, neck, shoulder, wrist, ankle/feet, knee, hip/buttocks and elbows were 59.7%, 47.9%, 38.1%, 33.7%, 26.9%, 25.4%, 15.2%, and 14.9%, respectively in one year period. There were significant differences of morbidities for musculoskeletal symptoms in body sites of workers among different factories (P < 0.05 or P < 0.01). The morbidities of musculoskeletal symptoms in elbows, waist, wrists and ankle/feet of the workers in refractory material and chemical fiber factories were higher than those in other factories, the morbidities for musculoskeletal symptoms of workers in garments and diamond factories were lower than those in other factories. The morbidities for musculoskeletal symptoms in neck, shoulders and wrists of female workers were significantly higher than those of male workers (P < 0.01). There were significant differences of the morbidities for musculoskeletal symptoms in body sites among workers with different educational levels (P < 0.05 or P < 0.01). There were significant differences of the morbidities for musculoskeletal symptoms in neck, shoulders, wrists, hip/buttocks and knee among groups with different age or different working years (P < 0.01), and the morbidities for musculoskeletal symptoms increased with age and working years. The proportions of unhealthy working postures and high working load among workers in refractory material and chemical fiber factories were higher; but those in garments and diamond factories were lower.

CONCLUSIONThe morbidities for musculoskeletal symptoms in waist, neck, shoulder and wrists of workers in manufacturing workers were higher; the gender, education level, age and working years could influenced the morbidities for musculoskeletal disorders.

Adult ; Female ; Humans ; Industry ; Male ; Musculoskeletal Diseases ; epidemiology ; Occupational Diseases ; epidemiology ; Posture ; Surveys and Questionnaires

OBJECTIVETo evaluate the quality of life in patients who had resection of oral cancer and reconstruction by radial forearm free flaps.

METHODSQuality of life of 49 patients was assessed by means of the 14-item oral health impact profile (OHIP-14) and the medical outcomes study-short form-36 (SF-36) questionnaires 12 months after operation.

RESULTSForty-one questionnaires were collected (84%). SF-36: the highest-scoring domain were physical role (92.9 ± 2.6) and bodily pain (82.6 ± 5.7), the lowest-scoring domain were vitality (61.5 ± 9.1), followed by role emotion (64.9 ± 6.8) and social functioning (65.2 ± 8.2). OHIP-14: the best-scoring domain were handicap (37.1 ± 15.1) and psychological disability (45.7 ± 11.9), the best-scoring domain were physical pain (64.2 ± 11.7) and functional limitation (61.9 ± 12.9).

CONCLUSIONSRadial forearm free flaps for reconstruction of oral defects after cancer resection could significantly influence the patients' quality of life.

Adult ; Aged ; Female ; Forearm ; surgery ; Free Tissue Flaps ; Humans ; Male ; Middle Aged ; Mouth Neoplasms ; surgery ; Postoperative Period ; Quality of Life ; Reconstructive Surgical Procedures ; methods ; Skin Transplantation ; Surveys and Questionnaires