Anti-Bacterial Agents ; adverse effects ; Child, Preschool ; Clindamycin ; adverse effects ; Humans ; Infant ; Male ; Paraplegia ; chemically induced

OBJECTIVE To explore the process safety management of the details of its application,storage and disinfection.METHODS Poutine managements of every link of the EO disinfection Process such as the responsibility,operation procedure,regularly inspection,etc were carried out.RESULTS There were no accident occured since April,2004,when the EO disinfection machine began to work.Every monitoring sign was within the permitted scope.CONCLUSIONS Strictly management of every key procedure is the safety guarantee for the whole process of EO disinfection.

OBJECTIVE To explore the methods of management of ethylene oxide disinfection and evaluate its effects.METHODS The biological and chemical indicatons were used to monitor the effects in the disinfection process.The operating procedures were carried out strictly.RESULTS In 150 times disinfection processes 4.6% were not up to the standards in the two kinds of indicators.The main reason was incorrect operations in the whole process.CONCLUSIONS Strictly management and correct monitoring are very important to improve the disinfection quality for safety operation.

OBJECTIVE Employing biological and chemical monitoring methods in steam sterilization qualification tests,to assure the qualification of the steam sterilizers. METHODS BI PCD and B-D test pack for three consecutive testings in empty chamber were used to qualify steam sterilizers. RESULTS Biological and chemical monitoring methods were the most effective monitoring techniques for steam sterility assurance. CONCLUSIONS The qualification tests should be executed exactly with the introduction of steam sterilizer in OR sterile supply division to assure medical safety.

Objective To investigate the predictive values of hypoxia-induced factor(HIF)- 1α and vascular endothelial growth factor (VEGF) in patients with type 2 diabetic kidney disease, and its relationship between HIF-1α, VEGF and glomerular filtration rate (eGFR). Methods Seventy-six patients with type 2 diabetic kidney disease were divided into two groups:DKD1 group [eGFR>90 mL/(min·1.73 m2),n=48] and DKD2 group [60 mL/(min·1.73 m2)0.05). Serum levels of HIF-1α and VEGF were significantly higher in DKD2 group than those of DKD1 group (P<0.05). There were negative correlation between VEGF and HIF-1αlevels with eGFR level (r=-0.59, P<0.01;r=-0.55, P<0.01). ROC curve showed that serum VEGF and HIF-1αlevels were above the opportunity diagonal. The diagnostic sensitivities of HIF-1 to DKD1 and DKD2 were 92.1%and 87.4%. The diagnostic specificities of HIF-1 to DKD1 and DKD2 were 91.7%and 85.4%. The diagnostic sensitivities of VEGF to DKD1 and DKD2 were 96.1%and 86.2%, and specificities were 93.3%and 89.1%. Conclusion Serum levels of VEGF and HIF-1αchange with the severity of diabetic nephropathy are likely to be an early predictor for the progression of diabetic kidney disease.

Adult ; Ear Canal ; Foreign Bodies ; Humans ; Male ; Mandibular Condyle

Objective To analyze the clonal gene rearrangement and complementarity determining region 3 (CDR3) repertoire of TCR β-chain in fine needle aspiration biopsy (FNAB) specimens of lymph node metastasis in patients with breast cancer. Methods The TCR CDR3 region genes of 24 TCR Vβ subfamilies were amplified by utilizing RT-PCR technology, and the CDR3 lengths of TCR β-chain were analyzed with gene scan technology for 2 cases with lymph node reactive hyperplasia and 3 patients with lymph node metastasis of breast cancer. The clonality of T cells presumed by spectra typing was further confirmed by CDR3 sequencing. Results TCR β-chain presented specific repertoire skewing in metastatic lymph node,and only 3-5 TCR Vβ subfamily of T cells were identified, respectively. Clonal expanded T cells, including oligoclonal, polyclonal patterns, in one or more Vβ subfamilies were found in all cases. The oligoclonal expanded T cells had different CDR3 amino acid sequences. Conclusion There are characteristic T cells cloning proliferation and selected usage of TCR Vβ subfamily T cells could be found in metastatic lymph node.The sequences of CDR3 in different TCR clone proliferation are mostly different.

Xanthelasma palpebrum is the most common type of xanthoma that develops mainly on the inner epicanthus of upper eyelids with symmetrical distribution. The lesion tends to be permanent, progressive and cosmetically disfiguring. The recommended treatment has been surgical excision, local treatment with chemicals, and carbon dioxide laser. All of the mentioned treatment have some disadvantages including scarring, pigmentary change and need for local anesthesia. We describe the treatment of xanthelasma palpebrum with the pulsed dye laser which coagulates the hyperpermeable vessels so that the lipid leakage could be blocked and prevent recurrence and further progression.
Anesthesia, Local ; Cicatrix ; Eyelids ; Lasers, Dye* ; Lasers, Gas ; Recurrence ; Xanthomatosis

OBJECTIVE To investigate the biological and molecular biological characteristics of Klebsiella pneumoniae in burn patients in order to give the first hand information for preventing and controlling of hospital acquired infections.METHODS The identification was done by Bio-Merieux ATB expression.Antimicrobial susceptibility test was performed with K-B method.The plasmid DNA was extracted by Alkaline Lysis,and separated by electrophoresis on the gel.The ESBLs detection was based on NCCLS.RESULTS The K.pneumoniae from the burn patients and the environment were sensitive to CIP,FOX and IPM,but showed resistance to the rest 12 antibiotics.The plasmid DNA profile analysis showed 3 types,and the relative molecular mass was approximately 4.7?106,3.6?106 and 2.0?106.The molecular biological characteristics showed these pathogens were ESBLs-producing K.pneumoniae,which was different from the control bacteria.At the same time,the pathogens caused the original infection were detected,and they were accordingly Staphylococcus aureus,Streptococcus pyogenes,and Pseudomonas aeruginosa.CONCLUSIONS The outbreak in burn patients is caused by ESBLs-producing K.pneumoniae,which has the same antibiotic resistance spectrum and plasmid DNA profile.This ESBLs-producing K.pneumoniae has the same origin.The pathogen might be transmitted by the case history clips and the door knobs.It was suggested that something must be done to enhance the antisepsis administration in order to prevent the hospital acquired infection.

Wet age-related macular degeneration (AMD) is one of major causes of blindness in elder people.Intraocular injection of anti-vascular endothelial growth factor (VEGF) -A regimen has made big breakthrough for the treatment on choroidal neovascularization of wet AM D,while long-term follow-up and necessary retreatments are the key issues to remain obtained visual acuity.Multiple strategies of wet AMD have been used in following-up and retreating based on the visual acuity,optical coherence tomography (OCT),ophthalmoscope and fluorescein fundus angiography (FFA) in abroad.However,there also are major differences in the patient' s composition,treatment habits and distribution of medical sources in China from Western.So we suggest to standardize the follow-up and retreatment strategies about intravitreal injection of VEGF-A for wet AMD as to achieve a better effectiveness.OCT-guided individual follow-up and retreatment strategies should be very helpful for maintaining a long-term efficacy,minimizing the treatment time and reducing medical cost.