1.Application of critical-care pain observation tool for the evaluation of pain in elderly patients with mechanical ventilation
Meng CHUN ; Zhang YAN ; Liang YU
Chinese Journal of Geriatrics 2011;30(12):1008-1011
ObjectiveTo assess the degree of pain in elderly patients with mechanical ventilation in ICU using critical-care pain observation tool(CPOT) and to choose the correct sedative and analgesic method.Methods 110 elderly patients in ICU after neurosurgery were divided into three assessment stages,every stage had two record points and total six points (T1-T6):the first stage (intubation and unconsciousness,T1-T2),the second stage (intubation and consciousness,T3T4 ) and the third stage(extubation and consciousness,T5-T6 ).Among them T1,T3and T5were nonnocuity assessment points of every stage,while T2,T4 and T6 were nocuity assessment points of every stage.The assessment time was one minute at every point.After recorded at every point in second and third stages,patients were asked to use the pain intensity descriptive scale (PIDS) themselves.CPOT,heart rate and mean arterial pressure (MAP) from T1 to T6 were recorded as well as PIDS from T3 to T6 in second and third stages.Results In the three stages,CPOT〔(26.8 vs.0.54,3.36 vs.1.20,2.78 vs.0.68) scores〕,HR〔(95 vs.85,94 vs.82,94 vs.84)beat/min〕 and MAP〔(95 vs.85,95 vs.87,94 vs.87)mm Hg〕 at T2,T4and T6 were higher than T1 (t=-42.89,-55.95,-55.38),T3 (t =- 5.52,- 11.33,- 11.78) and T5 ( t =- 5.54,- 9.95,- 11.33 ) ( P< 0.05 ).The PIDS at T4 and T6were higher than at T3and T5in the second and third stages 〔(2.52 vs.1.69,2.12 vs.1.44)scores〕 (P<0.05).The correlation coefficient between CPOT and PIDS at T3 and T4 in the second stage were 0.49 and 0.58,respectively (P<0.05),and between CPOT and PIDS at T5 and T6 were 0.52 and 0.59 in the third stage,respectively (P < 0.05),and they both reached moderate correlation.ConclusionsCPOT may be an effective way to assess the degree of pain in elderly patients with mechanical ventilation at present.
2.Effects of moderate exercise on lymphocyte number and Th1/Th2 balance in rats under chronic psychological stress
Yan ZHAO ; Chun WANG ; Yu WANG
Chinese Journal of Tissue Engineering Research 2009;13(50):9933-9936
BACKGROUND: Previous studies have proved that stress can affect immunity function and disturb homeostasis of bodies. Moderate exercise can alleviate the psychological stress reaction to some degree.OBJECTIVE: To observe the effects of moderate exercise on lymphocyte number in spleen and mesenteric lymph nod,interferon-y (IFN-γ) and interleukine-4(IL-4) level in serum, as well as Th1/Th2 balance in rats under chronic psychological stress.DESIGN, TIME AND SETTING: A randomized control animal experiment was carried out in the laboratory of Sports Human Science in Chengdu Sports University from May to October in 2007.MATERIALS: A total of 40 healthy male SD rats were divided by random digits table into 4 groups: control group, psychological stress group, exercise group and exercise+psychological stress group, with 10 ones in each group.METHODS: Before experiment, rats in exercise group and exercise+psychological stress group underwent 3 days of adaptive swimming training, 30 min/(t·d). During experiment, they were made swimming with no weight loading for 8 weeks, 60 min/(t·d).From day 2 post exercise on, rats in exercise+psychological stress group began to undergo the 21 days of chronic restraint stress,6 hours per day. At the same time, the chronic restraint stress models were established in the psychological stress group. Rats in the exercise group were fed for 22 days since the 8~(th) day of exercise ended. Rats in the control group were only fed without any intervention.MAIN OUTCOME MEASURES: The lymphocyte number in spleen and mesenteric lymph node was measured, and ELISA was employed to measure IFN-y and IL-4 levels as well as the ratio of IFN-y/IL-4 in serum.RESULTS: Compared with the control group, the lymphocyte number, the level of IFN-y and the ratio of IFN-y/IL-4 were significantly decreased in psychological stress group (P < 0.01 ), and the Thlf'rh2 balance was broken. Compared with the psychological group, the exercise+psychological stress group had got significantly higher lymphocyte number, IFN-γ level and ratio of IFN-γ/IL-4 (P<0.05). The lymphocyte number in spleen and mesenteric lymph node in the exercise group showed not a difference of significance compared with the control group, but a tendency towards going up. There was no difference of significance in IFN-γ level and ratio of IFN-γ/IL-4 among the exercise+psychological stress group, the exercise group and the control group.CONCLUSION: Moderate exercise can increase lymphocyte number in spleen and mesenteric lymph node significantly,up-regulate the IFN-γ level so as to regulate the disbalance of Th1/Th2, and maintain immunity homeostasis.
3.Solid State Fermentation Bioreactor
Chun-Yan LIAO ; Yu-Guo ZHENG ;
Microbiology 1992;0(01):-
Solid state fermentation involves the growth of microorganisms on moist solid substrates in the absence of free flowing water. It has gained considerable attention of late years due to its several advantages over submerged fermentation. This paper mainly introduces the characteristics and mathematical models of solid state fermentation and summarizes the development situation of various bioreactors, the design standards and applications.
4.Preparation of total alkaloids from Sophora alopecuroides freeze-dried powders and investigation of its antitumor effects.
Jia-Fu YAN ; Yan TONG ; Chun PU ; Jin-Yu WANG
China Journal of Chinese Materia Medica 2014;39(7):1234-1237
OBJECTIVETo investigate molding technology of total alkaloids from Sophora alopecuroides freeze-dried powders and observe its inhibition effects on liver transplantation tumor in mice.
METHODWith color, clarity, water-soluble and formability as indexes, single factor tests were adopted to screen type and amount of filler, the concentration of total alkaloids in drug liquid, pH in order to determine optimum prescription of total alkaloids from S. alopecuroides freeze-dried powders, the lowest melting point was determined and freeze drying curve was drafted. Mice hepatoma H22 ascites tumor strain was sterile inoculated in right axillary subcutaneous of mice, and antitumor effect of total alkaloids from S. alopecuroides freeze-dried powders on liver transplantation tumor H22 in mice.
RESULTWhen selected 80 g x L(-1) as mannitol as filler, the concentration of total alkaloids in drug liquid was 25 g x L(-1) and pH 6.5-7.5, freeze-dried effect was optimum with fast reconstitute speed. Average inhibition rate of the big (120 mg x kg(-1)) and medium (60 mg x kg(-1)) dose group of total alkaloids from S. alopecuroides freeze-dried powders on liver transplantation tumor H22 in mice were 56.08% and 35.49%, respectively.
CONCLUSIONPreparation technology was reasonable, reproducible and stable, total alkaloids from S. alopecuroides freeze-dried powders had significant antitumor effect and showed a dose-effect relationship.
Alkaloids ; chemistry ; pharmacology ; Animals ; Antineoplastic Agents, Phytogenic ; chemistry ; pharmacology ; Cell Line, Tumor ; Color ; Freeze Drying ; Humans ; Liver Neoplasms ; drug therapy ; Mice ; Powders ; chemistry ; Sophora ; chemistry ; Tumor Burden ; drug effects
5.Cloning and Prokaryotic Expression of Human Recombinant Calreticulin
Chun-Yu CAO ; Yu HAN ; Yan-Lin WANG ;
China Biotechnology 2006;0(04):-
Objective: Clone, express and purify human recombinant calreticulin (CRT). Methods: Human CRT cDNA was amplified from total RNA of human lung cancer cell line A549 cells by RT-PCR. Then, PCR product was subcloned into prokaryotic expression vector pET-15b. After sequencing, this recombinant plasmid was transformed into E.coli. Rossetta. Recombinant CRT was expressed in host cells by IPTG induction. Resulted protein was purified by Ni-NTA resin under denature condition and dialyzed to recover its native structure. SDS-PAGE and Western blot method were used to identify the expression and purification of reconbinant CRT. Results: Human CRT cDNA was cloned from total RNA of A549 cells. CRT prokaryotic expression vector pET-15b-crt was constructed. Reconbinant CRT was induced to express in E.coli and purified by Ni-NTA affinity chromatograph. Conclusion: A method for prokaryotic expression and purification of human recombinant CRT was successfully established. This method laid a foundation for the subsequent CRT research.
6.Discussion on relationship between soft tissue tension and cervical physiological curvature change of patients with scapular muscle fasciitis.
Yong ZHAO ; Wei FANG ; An YAN ; Gang WANG ; Chun-Yu LIU
China Journal of Orthopaedics and Traumatology 2014;27(5):376-378
OBJECTIVETo discuss the relative association of soft tissue tension and cervical physiological curvature chang in patients with scapula muscle fasciitis.
METHODSFrom February 2012 to December 2012,29 patients with scapula muscle fasciitis were investigated. There were 10 males and 19 females with an average age of 27.77 years old (ranged from 22 to 40 years old). Routine AP and lateral cervical X-rays were done in all patients. Cervical lordosis was measured according to Borden's method and the pain point tension was measured by soft tissue tension meter. Finally,perform statistic analysis to bove data.
RESULTSLateral X-rays showed 9 cases were normal cervical lordosis, 18 cases were cervical lordosis decreased, 2 cases were cervical lordosis increased. The regression equation of cerical lordosis changes D(Y) and soft tissue tension displacement D0.5 kg (X) was Y = -15.069 + 3.673X.
CONCLUSIONThere is linear relationship between soft tissue tension and cervical physiological curvature change. With the soft tissue tension increases, the cervical lordosis trend to decrease.
Adult ; Biomechanical Phenomena ; Cervical Vertebrae ; pathology ; physiopathology ; Fasciitis ; pathology ; physiopathology ; Female ; Humans ; Male ; Mechanical Phenomena ; Scapula ; pathology ; physiopathology ; Young Adult
7.Levels of interleukin-23 in asthmatic children before and after glucocorticosteroid treatment.
Yan-chun LI ; Ji-rong LU ; Shan-yu LI
Chinese Journal of Pediatrics 2007;45(3):218-219
Adolescent
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Anti-Asthmatic Agents
;
therapeutic use
;
Asthma
;
blood
;
drug therapy
;
Child
;
Child, Preschool
;
Female
;
Glucocorticoids
;
therapeutic use
;
Humans
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Infant
;
Interleukin-23
;
blood
;
Male
9.Rhinocerebral mucormycosis: report of two cases.
Chun-yan HE ; Yu-lan JIN ; Hong-gang LIU
Chinese Journal of Pathology 2010;39(5):345-346
Adolescent
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Aged
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Aspergillosis
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microbiology
;
pathology
;
Aspergillus
;
isolation & purification
;
Brain Diseases
;
drug therapy
;
microbiology
;
pathology
;
surgery
;
Diabetes Complications
;
microbiology
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Diagnosis, Differential
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Female
;
Humans
;
Male
;
Mucorales
;
isolation & purification
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Mucormycosis
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drug therapy
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pathology
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surgery
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Nose Diseases
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drug therapy
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microbiology
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pathology
;
surgery
10.Clinical observation on acupoint pressure plus long-snake moxibustion for upper-limb spastic hemiplegia after cerebral infarction
Yu-Chun WANG ; Hai-Yan SUN ; Fu-Qing ZHANG
Journal of Acupuncture and Tuina Science 2021;19(3):187-192
Objective: To observe the clinical efficacy of acupoint pressure plus long-snake moxibustion for upper-limb spastic hemiplegia after cerebral infarction. Methods: A total of 100 patients were randomized into a control group and an observation group, with 50 cases in each group. Both groups were treated with the same conventional internal medicine and rehabilitation training. The control group was treated with additional acupoint pressure therapy, and the observation group was treated with long-snake moxibustion on the basis of the treatment given to the control group. The Ashworth grade, Fugl-Meyer assessment upper limb scale (FMA-UL) and Barthel index (BI) were evaluated, and the root mean square (RMS) values of biceps brachii and flexor carpi radialis on the affected side were measured before and after treatment. The efficacy was evaluated after treatment. Results: After treatment, the total effective rate of the observation group was significantly higher than that of the control group (P<0.05). After treatment, the Ashworth grade of the observation group was superior to that of the control group (P<0.05). The scores of FMA-UL and BI in both groups increased compared with those before treatment (all P<0.05), and the scores of FMA-UL and BI in the observation group were higher than those in the control group (both P<0.05). The RMS values of biceps brachii and flexor carpi radialis in both groups decreased compared with those before treatment (all P<0.05), and the RMS values of biceps brachii and flexor carpi radialis in the observation group were lower than those in the control group (both P<0.05). Conclusion: Based on conventional internal medicine and rehabilitation training, acupoint pressure plus long-snake moxibustion has great therapeutic efficacy for upper-limb spastic hemiplegia after cerebral infarction. It can improve the degree of spasticity of the affected upper limb, reduce the muscle tone of biceps brachii and flexor carpi radialis on the affected side, and enhance the mobility of the affected limb and the activities of daily living.