Objective:To investigate the direct effect of different concentrations of hydrogen sulfide(H_2S)on neonatal rat myocardial cells by hypoxia injury during different time point,and the indirect effect of reoxygenation injury in order to analyze the protective role of H_2S on myocardial cells subjected to hypoxia/reoxygenation injury. Methods:Primary cultured myocardial cells from neonatal rats were randomly divided into 9 groups:Normal control group,Hypoxia NaHS 0 μmol/L group,100 μmol/L group,200 μmol/L group,400 μmol/L group and those groups subjected to hypoxia for 24 h,48 h and 72 h. Hypoxia/reoxygenation NaHS 0 μmol/L group,100 μmol/L group,200 μmol/L group,400 μmol/L group and those groups subjected to hypoxia for 24 h,48 h,72 h and then reoxygenation for 2 h. The number of survival cells were counted and the activity of lactate dehydrogenase(LDH)were measured respectively at each time point. Results:At the same time point of 24 h,48 h,72 h hypoxia,the number of survival cells increased and the activity of LDH decreased in hypoxia NaHS treatment groups as compared with Hypoxia NaHS 0 μmol/L group(P<0.01). The protective role of Hypoxia NaHS 200 μand 400 μmol/L groups were better than NaHS 100 μmol/L group at 24 h of time poit(P<0.05～0.01). The number of survival cells by hypoxia/reoxygenation increased and the leakage of LDH from reoxygenation cells decreased in hypoxia/reoxygenation NaHS treatment groups as compared with hypoxia/reoxygenation NaHS 0 μmol/L group(P<0.01).Conclusion:NaHS 100 to 400 μmol/L had the protective effects on myocardial cells by hypoxia/reoxygenation injury. NaHS 200 to 400 μmol/L might offer the best protective effects on myocardial cells by hypoxia injury at 24 h.

Objective To observe the acute toxic reaction and death of mice one day after intragastric administration of Baicalin capsule so as to appraise its safety. Methods The ICR mice were intragastrically administered with Baicalin powder in capsule at maximum concentration. There was no death of mice found and no LD_ 50 detected after administration. Hence the maximum dosage was identified. Results The maximum dosage of Baicalin powder is 15g/kg. Conclusions The Baicalin capsule is relatively safe.

Objective:To investigate the feasibility of dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI)parameters in differentiating musculoskeletal tumors with different behaviours of pathological findings before therapy.Methods:A total of 34 subjects of musculoskeletal tumors were in-volved in this retrospective analysis.DCE-MRI was performed using a fat-saturated 3 D VIBE (volumetric interpolated breath-hold exam)imaging sequence with following parameters:FA,10 degree;TR/TE, 5.6/2.4 ms;slice thickness,4.0 mm with no intersection gap;field of view,310 mm ×213 mm;ma-trix,256 ×178;voxel size,1 .2 mm ×1 .2 mm ×4.0 mm;parallel imaging acceleration factor.The ac-tuation time for the DCE-MRI sequence was 255 s with a temporal resolution of 5 s and 40 image vo-lumes.Using pathological results as a gold standard,tumors were divided into benign,borderline and malignant tumors.Toft’s model was used for calculation of Ktrans (volume transfer constant),Ve (extra-vascular extracellular space distribute volume per unit tissue volume)and Kep(microvascular permeability reflux constant).Those parameters were compared between the lesions and the control tissues using paired t-tests.The one-way analysis of variance was used to assess the difference among benign,border-line and malignant tumors.P values <0.05 difference was statistically significant.Results:Based on the WHO Classification of Tumours of Soft Tissue and Bone(2012)criteria,34 patients were divided into three groups:1 1 for benign tumors,12 for borderline tumors,and 1 1 for malignancies.Compared with control tissues,Ktrans and Kepshowed no difference,but Ve was increased in benign tumors,Kep showed no diffe-rence,butKtransandVewereincreasedinborderlinetumors, Ktrans,KepandVewereincreasedin malignant tumors.Ktrans(P<0.001 )and Kep (P<0.01 )were significantly higher in malignant tumors than in benign and borderline tumors,but did not show any difference between benign tumors and border-line tumors.Ve was significantly higher in malignant tumors than in benign (P<0.05),but did not show any difference between malignant and borderline tumors,benign tumors and borderline tumors (P >0.05 ).Conclusion:DCE-MRI technique is useful to evaluate the pathological behaviour of musculoske-letal tumors.The quantitative analysis of DCE parameters in conjunction with conventional MR images can improve the accuracy of musculoskeletal tumor qualitative analysis.

Objective: To determine if fusion genes of HSV-tk gene and cytokine gene have synergy on the cell killing of the Hep-2 human laryngeal carcinoma cell line in vitro. Methods: Different fusion genes expressing vectors PL(TI) SN, PL(TT)SN and PL(TK)SN were generated by recombinant DNA technology. Hep-2 was infected by the recombinant retrovirus. The positive clones were obtained after G418 selection and were termed Hep/TI, Hep/TT and Hep/TK respectively. The integration and expression of fusion genes in Hep-2 cells were identified by RT-PCR and Southern blot. The growth state and GCV killing effect of fusion genes modified cells were used to investigate the expression of fusion genes and antitumour effect on Hep-2 cells. Results: RT-PCR and Southern blot analysis confirmed the integration and expression of fusion genes in Hep-2 cells. There was no significant difference in cell proliferation between the Hep/TI and Hep/ TK.but the growth of Hep/TT was restrained. After the treatment of GCV the Hep/TI, Hep/TT and Hep/TK all showed high sensitivity to GCV. The killing effect of GCV on Hep/TT was the most siginificant and bystander effects were observed siginificantly in vitro. Conclusion: The fusion genes of HSV-tk and cytokine gene have synergistic effects on killing Hep-2 cell after treatment of GCV in vitro,which might have therapeutic potentials for laryngocarcinoma.

Objective To investigate relationship between different degrees of asymptomatic carotid artery stenosis and cognitive impairment. Methods The cognitive function were evaluated with the montreal cognitive assessment scale (MoCA) in 80 patients with asymptomatic carotid artery stenosis and 56 controls, the total MoCA scores and subprojects scores were compared between the patients with different degrees of ACS and controls. Results The total MoCA score, visual space and executive function, attention and delayed recall had significant difference among the mild, moderate, severe ACS patients and control group (all P < 0.05). There was no statistical significance of the total MoCA score and the scores of all subprojects between the patients of mild ACS patients and the control group (all P > 0.05). The total MoCA score and the scores of visual space and executive function , attention and delayed recall in moderate and severe ACS patients were significantly lower than the control group and mild ACS patients (P < 0.05). Furthermore, the severe ACS patients, compared with the moderate ACS patients , performed worse on the cognitive tasks which involved visual space and executive function, attention and delayed recall (P < 0.05). Conclusions Cognitive function is impaired in the patients with moderate and severe ACS,the neuropsychological characteristics are mainly impaired in visual space and executive function, attention and delayed recall.

Objective To study the risk factors related to early neurological deterioration (END) in patients with recurrent cerebral infarction (RCI). Methods Three hundreds and twenty-six RCI patients admitted to our hospital from January 2014 to March 2015 were divided into END group (n = 66) and non-END group (n =260). The clinical and laboratory data were collected, and their cerebrovascular lesions were accessed according to the angiographic findings. Results (1) Single factor analysis showed the level of cholesterol was significantly higher in the END group than that of the non-END group (P < 0.05). The proportion of patients suffering from smoking, arterial occlusions, early onset stroke associated pneumonia (SAP) and hyponatremia was also higher in the END group (all P < 0.05). (2) Logistic regression analysis showed that arterial occlusions (OR = 2.137, 95%CI 1.093~4.181), SAP (OR=2.001, 95%CI 1.023~3.912) and hyponetremia (OR=2.467, 95%CI 1.217~ 5.000) were independent risk factors for END of RCI patients (all P < 0.05). Conclusion Arterial occlusions, SAP and hyponatremia may be used as predictors of the early development of RCI patients.

Objective To report 49 cases of adult interatrial septal defect with pulmonary hypertension operated and to evaluate their results.Method Most of those cases were repaired with conventional operative procedures,but 2 cases among them with severe pulmonary hypertension were repaired with one-way valvular patch.Those with mitral or tricuspid remarkable regurgitation,were repaired with annuloplastic procedure at the same time.Results There were no operative death,and followed up for one to five months,the heart size,ECG and the data of blood gas analysis were retuned into normal value.Heart function of 47 case was raised to Ⅰ class(NYHA),and 2 case to Ⅱ class.Conclusions Operation for adult interatrial septal defect especially with pulmonary hyperteasion is highly indicated.Treatment of mitral or tricuspid regurgitation remarkably at the same time is important for recovery of cardiac function.One-way valvular patch used to repair ASD with severe pulmonary hypertension could reduce complication and mortality,so it is an effective procedure adapted.

[Objective] To investigate the role of exenatide on angiogenesis in endometrial cancer Ishikawa xenografts in nude mice.[Method] We used the subcutaneous human endometrial cancer cell Ishikawa xenografts in nude mice model,and divided them into control group and exenatide-treated group.The harvested tumors were preserved for immunohistochemistry staining and western blot analysis.[Results] The micro-vessel density (CD31 positive) in exenatide-treated group was (13.2 ± 1.4)/400 power field,less than that in control group [(25.9 ± 5.8)/400 power field] (P ＜ 0.01).The expression level of VEGF was significantly lower in exenatide-treated group than that in control group (P ＜ 0.05).The mean density of tumor associated macrophages (F4/80 positive) is (31.4 ± 3.4)％ in exenatide-treated group and (72.1 ± 4.2)％ in control group with significant difference (P ＜ 0.01).[Conclusion] Exenatide weaks tumor angiogenesis within endometrial cancer Ishikawa xenografts in nude mice.

Protein transduction is an emerging technique in biological fields in recent years. Through the medium of protein transduction domain(PTD) composed by a small number of alkaline peptides, the functional proteins linked covalently with DNA, peptides, proteins or other macromolecules can across the membranes of mammalian cells, even the blood-brain barrier and transfer unconventionally that isn't like classical pathway. The distinct property of PTD that can take along fused protein entering into cells unconventionally offers a new vehicle to gene therapy for some diseases and has dulcet applied perspective.

Objective To provide a series of surgical approaches for treatment of bone lesions in ankle and foot. Methods Based on the anatomic investigations,vascularized cuboid bone,medial cuneiform bone,navicular bone and lateral part of calcaneum bone grafting were designed for repaired bone lesions in the area of ankle and foot,and applied to 55 clinic cases. Results Forty-eight cases among them were followed up from 1 year to 10 years,4 years and 6 month in average,the results were satisfactory. Conclusion The designed four types of vascularized tarsal bone flaps are easy and reliable for dissection because of their superficial pedicle.