Influenza viruses are global epidemic and diversely difficult to distinguish,which threaten human’s survival and development very much.In recentyears,the frequent outbreaks of influenza prompt the rapid development of Influenza virus detection.Compare with the traditional isolated culture and immunological detection,molecular diagnostic technology is of high detection speed,high sensitivity and specificity,that gradually play an important role in the current Influenza virus de-tection.In order to provide a theoretical basis for the rapid diagnosis of Influenza virus in the clinic,the article summarize the update progress of molecular biology and diagnostic techniques of Influenza viruses.

Cellular functions like proliferation,differentiation,migration,morphogenesis and apoptosis are modulated by the extracellular matrix.Integrins are the prototypic heterodimeric transmembrane matrix receptors with competing affinities for individual extracellular matrix ligands.The intracellular integrin domain clusters cytoplasmic proteins into focal adhesion plaques for bidirectional(outside-in and inside-out) signaling.Integrin-linked kinase(ILK) is an intracellular serine/threonine protein kinase that interacts with the cytoplasmic domains of ?-integrins.ILK organizes the connections of the extracellular matrix via integrins to the cytoskeleton and is involved in adhesion plaque signaling,and it plays a crucial role in the pathogenesis of chronic renal fibrosis.High glucose induces the upregulation of the synthesis and activity of ILK in cultivated mouse podocytes and mesangial cells.The ILK protein level is significantly increased in diabetic glomeruli.ILK activity is likely to provide the basis for an effective therapeutic method for diabetic nephropathy.This review gives an introduction of ILK structure and function,followed by a summary of our current understanding of ILK in diabetic nephropathy with a special focus on glomerular cell-matrix interaction.

Objective To explore the effects of insulin pump on pancreatic ?-cells in type 2 diabetes mellitus accompanied with hyperglycaemia.Methods We treated 46 cases of newly diagnosed type 2 diabetes mellitus with fasting blood glucose≥10.0 mmol/L by insulin pump for 2 weeks.Their blood sugar and insulin were determined before and after treatment by oral glucose tolerance test and insulin releasing test.We then calculated the function index,morning secretion of pancreatic ?-cells and insulin resistance index.The patients were followed up for 6 months and the blood glucose was monitored.Results After treatment,insulin,function index and the morning secretion were increased significantly,while the blood sugar and resistance index were decreased.In all the patients,16 could maintain ideal blood glucose only by alimentary control and exercise.Conclusion With the insulin pump in a short term,patients with type 2 diabetes mellitus can rapidly control blood glucose,obviously improve pancreatic ?-cells,and reduce insulin resistance.

Cardiovascular Diseases ; diagnosis ; genetics ; Humans ; MicroRNAs ; blood ; genetics

Dental Bonding ; Dental Implant-Abutment Design ; Humans ; Matrix Metalloproteinases

Aged ; Endocarditis ; etiology ; therapy ; Humans ; Kidney Transplantation ; adverse effects ; Male

Objective To investigate effect of guanosine diphosphate (GDP) on the mitochondrial respiratory oxygen consumption and the mitochondrial membrane potential (MMP) of rat brain and explore the relationship of the change of uncoupling proteins (UCPS) activity with the oxygen consumption and MMP. Methods The mitochondria of rat brain were isolated by centrifugation. Mitochondria oxidative respiratory consumption was measured by Clark electrode after the treatment of GDP at different concentrations so as to calculate mitochondrial state 3 respiration (ST3), mitochondrial state 4 respiration (ST4), respiratory control rate (RCR), and the rate of oxidative phosphorylation (OPR). MMP was detected by Rhodamine 123 method at the different concentrations of GDP. Results With the increase of GDP concentration form 0 to 1.0 mmol/L, the values of ST3, ST4 and OPR were reduced while RCR was elevated. But when the concentration increased to 1.4 mmol/L, the former 3 indexes begun to increase while the later declined. When the GDP concentration reached to 1 mmol/L, the inhibitory rate was only 35.1%, 51.3%, 14.2% to ST3, ST4 and OPR respectively, while RCR was increased to 133.2%. No matter the concentration was over 1 mmol/L or under 1 mmol/L, the ability of inhibition was attenuated. MMP reached to the highest point when GDP exerted the highest inhibitory rate on mitochondrial respiratory oxygen consumption. Conclusion GDP, an inhibitor of UCPS, can regulate the respiratory oxygen consumption and MMP of the isolated rat brain mitochondrial directly in a dose-effect fashion. The change of UCPS activity can affect the respiratory oxygen consumption and MMP.

Objective:To explore distinguishing value of serum aldosterone level for different types of atrial fibrilla-tion (AF) .Methods:A total of 153 AF patients were selected ,including 99 patients with paroxysmal AF (paroxys-mal AF group) and 54 patients with persistent AF (persistent AF group) .Serum aldosterone concentration was measured ,and its correlation with two types of AF was analyzed .Results:Compared with patients with paroxysmal AF ,there was significant rise in serum aldosterone level [ (234.3 ± 69.6) pg/ml vs .(302.7 ± 78.3) pg/ml , P<0.01] in patients with persistent AF .Correlation analysis indicated that aldosterone level was positively correlated with atrial diameter and left ventricular ejection fraction (r=0.896 ,0.831 ,P<0.01 both) .Conclusion:Serum al-dosterone level is helpful to distinguishing paroxysmal atrial fibrillation and persistent atrial fibrillation ,and posses-ses certain guiding significance for outcome .

Objective To evaluate the efficacy of supportive psychotherapy on patients with cardiac neurosis. Method 32 patients with cardiac neurosis were treated with a comprehensive measure consists of adequate drug therapy and supportive psychotherapy. Symptom Checklist 90 (SCL - 90) was rated. Result Among the 9 factors of SCL - 90, scores of somatization, obsessive - compulsive, interpersonal relationship, depression, anxiety and phobic anxiety factors were significanly higher than that of normal controls. After treatment, the scores of somatization, depression, anxiety and phobic anxiety were statistically reduced. Patient- reported improvement efficacy was 84. 38%.Conclusion Adequate drug therapy together with supportive psychotherapy has reliable efficacy on patients with cardiac neurosis.

Objective To evaluate development of postoperative complications of calcanens fracture by employing Evidence-based medicine method so as to provide basis for clinical treatment strategy. Methods Eligible literatures on surgical treatment of calcalneus fractures from 1989 to 2008 were retrieved through Medline, Cochrane Database, Embase Database and Science Direct. The retrieved literatures were organized and analyzed by using SPSS14.0 and RevMan5 software. Results A total of 1823 literatures on calcaneus fractures were originally obtained based on preset retrieval conditions. With screening and evaluation, 29 clinical reports involving 2 661 patients (2 800 feet) met the criteria of meta-analysis. The results of recta-analysis indicated that the incidence rates (IR) and confidence interval (CI) of the complications that included infection (IC =8.6%, 95% CI =7.53-9.61), wound-edge necrosis (IC =2.6%, 95% CI =2.05-3.24), subtalar arthritis (IC =2.0%, 95% CI = 1.42-2.44), peroneal tendinitis (IC = 1.3% , 95% CI = 0.84-1.66), vascular or nerve damage (IC = 1.2%, 95% CI 0.81-1.62), osteotitis of calcaneus (IC = 1.0% ,95% CI 0. 60-1.33), non-union (IC =0.2%, 95% CI = 0. 04-0. 39) , malreduetion (IC = 0. 1%) and painful implants (IC = 0.7% , 95% CI = 0.4-1.03) respectively. Conclusion The postoperative complications of calcaneus fracture are common clinical problems, to which professional care without delay can attain satisfactory outcome.