Cerebral Infarction ; drug therapy ; Drug Therapy, Combination ; Drugs, Chinese Herbal ; therapeutic use ; Humans ; Medicine, Chinese Traditional ; Neurology ; Parkinson Disease ; drug therapy ; Peripheral Nervous System Diseases ; drug therapy ; Phytotherapy

Objective To study computed tomography (CT) in evaluating prognosis for hypoxic ischemic encephalopathy (HIE) in infants. Methods Eighty-five infants diagnosed as HIE underwent CT one week,two months,three to six months and one to one and a half years after birth,respectively. At the same time,neonatal behavior neurological assessment (NBNA) was made at 12 to 14 days after birth. Development quotient (DQ) was used to evaluate their prognosis at age of 1 to 1.5 years. Results Rate of poor prognosis was 40.9% (9/22) in the infants with severe HIE by CT and 5.7% (3/52) in those with moderate HIE within one week after birth. Rate of poor prognosis was 60.0% in the infants with abnormal CT at age of two months. There was no significant difference in their prognosis for infants of moderate HIE with NBNA scores greater than or equal to 35 and less than 35,12 to 14 days after birth. And,72.7% (8/11) of the infants with severe HIE by CT with NBNA less than 35 showed little recovery at 12th-14th days after birth. Conclusions Combination of CT and clinical follow-up with NBNA score one month after birth could play an important role in the evaluation for therapy regime,length of treatment and prognosis in the infants with HIE.

Objective To observe the effects of interleukin-1 ?(IL-1?) on spontaneous electrical activity of neurons in paravenrticular nuclei,and discuss the possible mechanism.MethodsExtracellular recording technique was employed to observe the effects of IL-1? on spontaneous electrical activity of neurons in paravenricular nuclei in rat hypothalamic slices,and the influence of losartan on the electrical activity induced by IL-1? was examined.ResultsAfter exposure to IL-1?(1?10-7 mol/L),the discharge frequency was significantly increased in 46 of the 59 discharge units(78%) of neurons(P

Objective:To evaluate the application of analyzing neutrophil cell surface marker CD64 in diagnosis of premature infants infection.Methods:109 infants inpatient in neonatal department(including NICU)were enrolled in the study.CD64 was measured by FCM,which was compared with C-reactive protein(CRP)and IL-6.Results:There was a statistically significant difference in quantitation of CD64 on neutrophil cells(P

Objective To explore the clinical significance of monocyte chemoattractant protein-1(MCP-1)in children with Henoch-Schonlein purpura nephritis(HSPN).At the same time compare the association between serum and urine MCP-1,to investigate the impact of the both on them in children with HSPN.Methods Serum and urine MCP-1 were measured by enzyme linked immunosorbent assay in 50 children with Henoch-Schonlein purpura(HSP)(25 cases of them patients with renal injures),and 25 healthy children,the changes of serum and urine MCP-1 were compared;at the same time serum urea nitrogen,creatinine,urinary albumin,urine N-acetyl-D-glucosaminidase(NAG),urine ?2-MG,24 hours urinary levels of protein were investigated in children with HSPN by analyzing the correlation between these indicators and serum and urine MCP-1;urine MCP-1 in HSPN group were measured in recovery period,and were compared with urine MCP-1 in HSP group and HSPN group in acute period.Results 1.The expressions of urine MCP-1 was significantly higher in HSPN group than those in HSP group and healthy controls(P0.05).2.Urine MCP-1 levels were associated with proteinuria in children with HSPN,but serum MCP-1 levels had nothing to do with HSPN.3.There was a close correlation between urine MCP-1 expression and urinary albumin,urine NAG,urine ?2-MG and 24 hours urinary levels of protein,but the expression of urine MCP-1 levels were not correlated with the serum urea nitrogen and creatinine.4.There was statistical significance in urine MCP-1 in acute and recovery periods with HSPN group(P

Adolescent ; Child ; Child, Preschool ; Female ; Humans ; Male ; Mallory-Weiss Syndrome

Objective Based on the published articles,this paper aims to study the pattern and probability of lymph nodes metastasis in nasopharyngeal carcinoma (NPC) and establish a radiotherapy standard for selecting and delineation of clinical target volume of neck lymphatics for NPC patients with N0 or N1.Methods Clinical trials about NPC by MRI as the main or only diagnostic imaging way were collected and extracted from database,such as WanFang,CBM,PubMed,Cochrane Library.Statistical analyses were performed using Stata software,version 12.0.Results Twenty-one clinical trials from 963 original articles were included in this analysis.The most commonly involved regions included retropharyngeal (67％) and level Ⅱ lymph nodes (71％).The overall probability of levels Ⅲ,Ⅳ and Ⅴnodal involvement were 37％,14％ and 21％,respectively.Low-risk node groups included levels ⅠA,ⅠB,Ⅵ nodes,and the rates of lymph node metastasis were 0,2％,2％ and 1％,respectively.Nodal metastases followed an orderly pattern of top-down,and the probability of skip metastasis between levels varied between 0.5％ ～7.9％.Conclusion Lymph node metastasis in NPC follows a predictable and orderly pattern.The rarity of metastasis in certain nodal groups andskip metastasis suggest that reduced treatment volume is feasible in conformal radiotherapy for NPC patients with No or N1.

Aim To study the effect of Lacidophilus exopolysaccharides(LAEPS) on immunity. Methods Effects of LAEPS on immunity were investigated by delayed type hypersensitivity reaction, haemolytic plaque assay and macrophage function assay in mice. Results LAEPS ip 7～8 day promoted delayed type hypersensitivity reaction, increased haemolytic plaque and enhanced macrophage function in a dose-dependent manner.Conclusion LAEPS is able to enhances immunity.

Alzheimer disease (AD) is a common neurodegenerative disease in the elderly, but nowadays the pathogenesis of AD is unclear. Myeloid cell 2 trigger receptor (TREM2) is one of the most famous and most common rare mutations in neurodegenerative disease research, and its functional site mutation can significantly increase the incidence of AD. In this paper, we summary the structure, localization, and function and related signaling pathways of TREM2, review the latest epide?miological findings of TREM2 associated with the pathogenesis of AD, and speculate on the possible role of TREM2 in the progression of this disease, as well as the expression of TREM2 and the role of soluble TREM2 in AD brain are further elucidated. Based on the potential protective effect of TREM2 in the pathogenesis of AD, Therefore, targeting TREM2 may provide new opportunities and a reference for AD treatment. As the TREM2 variant appears to be widely involved in neurodegenerative diseases, there is an urgent need to further study the function of TREM2 in the brain and to find its ligands involved in TREM2-mediated signaling transduction and its specific role in AD pathogenesis.

BACKGROUND:Sema7A is a kind of cel surface protein, which can promote the fusion of osteoclasts and the migration of osteoblast at the same time, affecting the dynamic balance of bone. OBJECTIVE:To investigate whether Sema7A siRNA has ainhibitory effect on the osteoclast activation in the process of osteolysis which induced by titanium particles. METHODS:The precursor osteoclasts with the concentration of 4×109 RESULTS AND CONCLUSION:At 7 days of culture, the expression levels of interleukin-1, interleukin-1β, tumor necrosis factor α, matrix metaloproteinase-9 and the receptor activator of nuclear factor-κB in the positive control, /L were seeded on 96-wel plates containing glass cover slips, and divided into four groups: blank control, positive control, experiment and negative control groups. The cel culture medium was added into the control group. 20 μL un-transfected siRNA supernatant was added into the positive control group. 20 μL transfected Sema7A siRNA supernatant was added into the experiment group. 20 μL transfected control siRNA supernatant was added into the negative control group. The supernatant was obtained through the co-culture between titanium particles solution and monocyte-macrophage cel line RAW264.7of mouse for 24 hours. siRNA was transfected into mononuclear macrophage cel lines RAW264.7 of mice. negative control and experiment groups were higher than those in the control group (P < 0.05). The expression level of each factor in the experiment group was lower than that in the positive control and negative control groups (P < 0.05). At 8 days of culture, the proliferation activity of osteoclasts and the number of positive cels stained by tartrate-resistant acid phosphatase in the positive control, negative control and experiment groups were higher than those in the control group (P < 0.05). The proliferation activity of osteoclasts and the number of positive cels stained by tartrate-resistant acid phosphatase in the experiment group were lower than those in the control and negative groups (P < 0.05). These results demonstrate that Sema7A siRNA has a certain inhibitory effect on the osteoclast activation induced by titanium particles.