OBJECTIVE: How to promote the generation of neural stem cells (NSCs) and induce them differentiate into the goal cell type has been the focus of neuroscience. In this article, we review the adjustment of basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF) to the proliferation and differentiation of NSCs. DATA SOURCES: A computer-based online search of Medline database was undertaken to identify the related articles published in English from January 1998 to March 2006 by using the keywords of "bFGF, EGF, nerve stem cells". At the same time, Chinese relevant articles were searched in China National Knowledge Infrastruction published between January 2000 and March 2006 with the same keywords in Chinese. STUDY SELECTION: The data were selected firstly to look over the citations of every literature. Inclusive criteria: Articles related to the effect of bFGF and EGF on the proliferation and differentiation of NSCs. Exclusive criteria: Duplicate research or Meta analysis. DATA EXTRACTION: A total of 78 related articles were collected, of which 32 accorded with the inclusive criteria and 46 with duplicate or old contents literatures were eliminated. Among the 32 literatures, 8 involved in the research status of NSCs, 10 about the effect of bFGF on the proliferation and differentiation of NSCs and the mechanism, 4 involved in the effect of EGF on the proliferation and differentiation of NSCs and the mechanism, respectively, 8 related to the effect of bFGF and EGF together, and 5 related to the comparison of the effect of bFGF and EGF. DATA SYNTHESIS: ①NSCs are self-renewed, and can differentiate into different stem cells and mature tissue cells in different microenvironment. Many cytokines in the microenvironment play essential roles in the differentiation direction of NSCs. ②bFGF has strong improvement effect on the proliferation of NSCs and can activate the potential regeneration capacity of neuronal precursor cell in distinct areas of central nervous system, proliferation and differentiation of cells present bFGF concentration dependent. ③EGF can improve the growth of NSCs, and promote them differentiate into neuron and glial cells; its effect of facilitating cell proliferation also presents concentration dependent. ④To elevate the efficacy of proliferation and differentiation of NSCs, allied application of bFGF and EGF is usual in NSCs culture. ⑤There is not a unified cognition to the effect difference of bFGF and EGF. CONCLUSION: bFGF and EGF have powerful effect to improve the proliferation and differentiation of NSCs; therefore, both of them are important neural somatomedin. Moreover, their effects present concentration dependent.

S100B is a class of acid calcium-binding protein. It mainly exists in neuroglial cells in the central nervous system, and it is one of the signs of astrocyte activation. S100B may reflect the function of neuroglial cells and regulate the complex interactions between the neurons and neuroglial cells. Studies in recent years have demonstrated that S100B protein is involved in the pathological processes of acute ischemic brain injury. Serum S100B protein can be used as an important marker for identifying the severity of cerebral infarction, progress and outcome.

Female ; Humans ; Male ; Nasal Septum ; abnormalities ; surgery ; Otorhinolaryngologic Surgical Procedures ; Rhinitis, Allergic, Perennial ; complications ; surgery ; Treatment Outcome

Objective To explore the eorrelafion factors of health promotion lifestyle of the elderly los-ing their land and sea estate. Methods Using the health value scale, health promotion lifestyle profile and the general conditions scale to investigate the health value, health promotion lifestyle and general conditions of 85 old people losing their land and sea estate in Dalian development area. Results There was no correlation between health value and health promotion lifestyle of the eldly, age, education degree, number of children and previous employment were rehted with the health promotion lifestyle. Conclusions There was distance be-tween their thought and actual health behavior of the elderly, community management personnels and med-ical workers should strengthen the concrete intervention in lifestyle, not simply relying on propaganda of the importance of health.

Objective To evalute the feasibility of a double hand allogroft to reconstuct the limbs'function. Methods A brain dead donor was chosen under the test of blood group in ABO and Rh,HLA match,PRA and the mixed leukocyte culture.The level of the transplantated hands was 5 cm proximal to the wrists The procedure of the allotransplantation was similar to that of the autologous replantation.The immunosuppressants were taken two days before the operation,in the operation and after the operation. The circulation,the vital signs and the rejection were under the supervision. Results The progress was satisfactory. No rejection was found postoperation.After 10 weeks the callus in both ends of radius and ulna was obvious.After 5 months sensation recovered.After 7 months the internal fixation(plates and screws) was removed and tenolysis was done. Now the recipient could use engrafted hands to wash face,make the bed,wear clothes,brush teeth,peel oranges,eat with a spoon,tie the shoelace slowly,pick up a telephoneor and use the conroller of Television. Conclusions With the immunosuppressive agents,the double hands allograft can survive.The growth rate of bone and nerve is faster than that of autologous replantation.

AIM: To establish the GC-fingerprint chromatogram of Mylabris. METHODS: Mylabris from Guizhou Province was used under the conditions of Agilent Technoligies DB-1 capillary column and the program 160℃—2℃/min—210℃(10min), and the GC fingerprint chromatogram of Mylabris was set up. RESULTS: The methodological evaluation showed that this method had a good reproducibility, common peaks' relative areas of different samples were different. CONCLUSION: This method is reliable to evaluate the quality of Mylabris.

BACKGROUND:Among various seed cel s, synovial mesenchymal stem cel s have unique advantages in the repair of articular cartilage injury. OBJECTIVE:To review the progress of synovial mesenchymal stem cel s and its intra-articular injection in the treatment of articular cartilage injury. METHODS:The first author searched PubMed and CNKI by computer to retrieve articles published from January 2004 to December 2004 using the keywords of“synovial mesenchymal stem cel s;intra-articular injection;cartilage repair”in English and Chinese, respectively. Final y, 57 articles were included in result analysis. RESULTS AND CONCLUSION:It is easy to isolate and culture synovial mesenchymal stem cel s, which has great advantages in cartilage repair. What’s more, intra-articular injection therapy for articular cartilage injury is feasible and safe. Intra-articular injection of synovial mesenchymal stem cel s is a very promising treatment for cartilage damage, but there are stil many problems to be solved in the future.

Objective To analyse the clinical usage of Shenmai injection, promote the rational use of Shenmai injection. Methods 400 hospital medical records that used Shenmai injection in 2015 were collected and reviewed from hospital information system (HIS) by retrospective analysis method, aggregated and evaluated data by EXCEL. Results In 400 cases, 11 clinical departments used the Shenmai injection, mainly in cardiology of 347 cases(86.75%).The percentage of the non-standard clinical application of Shenmai injection was 84 cases (21.00%). The non-standardized rates of solvent dispensing was 287 cases (71.75%), and the specification incompatibility accounted for 105 cases (26.25%). There was no adverse drug reaction of Shenmai injection. Conclusion The clinical use of Shenmai injection is not yet standardized, which suggests that drugs should be strictly used in accordance with the instruction, in order to avoid risks of off-label use.

AIM: To establish a novel method for the overall quality control of Radix et rhizoma seu caulis Acanthopanacis senticosi on the ground of HPLC digitized fingerprint.METHODS: The chromatographic fingerprints were obtained by injecting 10 ?L of the sample solution each time on a Century SIL BDS column(20 cm?4.6 mm,5 ?m) with the gradient elution solvent system composed of 1% acetate acid water and 1% acetate acid acetonitrile.The flow rate was 1 mL/min,the colunm temperature was maintained at(30?0.15)℃ and the detection wavelength was set at 265 nm. RESULTS: 31 co-possessing peaks were selected as the fingerprint peaks of Radix et rhizoma seu caulis Acanthopanacis senticosi and the similarities between the chromatographic fingerprints and the herbal drugs were calculated taking chlorogenic acid peak as the reference peak.The chromatographic fingerprints also were evaluated by the chromatographic fingerprint index(F),the relative index(Fr). CONCLUSION: This method with good precision and reproducibility can be useful for the quality control of Radix et rhizoma seu caulis Acanthopanacis senticosi.

0.05).No sign of aseptic loosening or change in implant position was noted.[Conclusion]This short-term study showed that bipolar femoral head arthroplasty seems to be a suitable alternative for primary treatment of displaced femoral neck fractures in patients over 80 years.More attention should be paid to coexisting medical illness(e.g.diabetes mellitus,hypertension,and ischaemic heart disease)and prevention of complications.