1.Influence of Wnt signaling pathway on mouse NIT-1 β-cell cultured in vitro
Shuyan GUI ; Muxun ZHANG ; Lili ZHOU ; Yikai YU ; Gang YUAN
Chinese Journal of Endocrinology and Metabolism 2010;26(8):707-710
Objective To establish whether Wnt-signaling pathway plays a role in mice β-cell function and/or survival in vitro. Methods Mice NIT-1 beta cells were cultured in media with glucose concentration of 33.3 mmol/L and the cytokines interleukin-1β, interferon-γand tumor necrosis factor-α with or without the addition of purified Wnt3a protein in vitro. Subsequently, β-cell apoptosis by Tunnel and flow cytometry, and β-cell proliferation by BrdU were analyzed. Total RNA was extracted to measure gene expressions by real-time PCR.Results Incubations of NIT-1 cells with high glucose and cytokines resulted in an increase in β-cell apoptosis and decrease in β-cell proliferation (P<0.01). In contrast, treatment with Wnt3a protein protected β-cell from glucose and cytokines-induced apoptosis through up-regulating the expressions of above Pitx2、 TCF7L2. Conclusions Wnt-signaling regulates the proliferation of pancreatic β-cell, and protectes β-cell from glucotoxicity and cytokine toxicity with respect to proliferation and apoptosis.
2.Source Identification of Human Biological Materials and Its Prospect in Forensic Science
Kainan ZOU ; Cheng GUI ; Yu CAO ; Fan YANG ; Huaigu ZHOU
Journal of Forensic Medicine 2016;32(3):204-210
Source identification of human biological materials in crime scene plays an important role in reconstructing the crime process. Searching specific genetic markers to identify the source of different human biological materials is the emphasis and difficulty of the research work of legal medical experts in recent years. This paper reviews the genetic markers which are used for identifying the source of human biological materials and studied widely, such as DNA methylation, mRNA, microRNA, microflora and protein, etc. By comparing the principles and methods of source identification of human biological materials using different kinds of genetic markers, different source of human biological material owns suitable marker types and can be identified by detecting single genetic marker or combined multiple genetic markers. Though there is no uniform standard and method for identifying the source of human biological materials in forensic laboratories at present, the research and development of a series of mature and reliable methods for distinguishing different human biological materials play the role as forensic evi-dence which will be the future development direction.
3.Expression of Tpap gene in mouse testis
Aifa TANG ; Zhou YU ; Xiaoyan ZHANG ; Zhenming ZHANG ; Yaoting GUI ; Jiongxian YE ; Zhiming CAI
Chinese Journal of Tissue Engineering Research 2010;14(18):3335-3338
BACKGROUND: These serial processes for forming male gametes are basically controlled by the programmed expression of a number of stage-specific genes. However, many aspects of the mechanisms of spermatogenesis have remained elusive because of a lack of suitable in vitro or in vivo models.OBJECTIVE: To screen genes involved in spermatogenesis, and to analyze its expression characteristics. METHODS: Testes cDNA samples from Balb/C mice of different postnatal days (4,9,18,35, 54 days and 6 months, respectively) were hybridized with mouse whole genome Affymetrix chip to screen the testis-ralated genes. The characteristics of the selected genes were analyzed by various bioinformatics tools. RT-PCR was used here to identify the expression of the selected genes in mice testis.RESULTS AND CONCLUSION: The Affymetrix chip probe of mouse Tpap was graduated higher expression with developmental stages of mouse testis. The scaling hybridization signal intensities of the tested testis on days 4, 9,18, 35, 54, and 6 months of postnatal were 4.4 (Absent expression, A), 12.9 (A), 262.4 (Present expression, P), 1136.7 (P), 1617.5 (P) and 1128 (P),respectively. These results indicated that the expression of mouse Tpap wasn't detected on days 4 and 9, but was detected on days 18, 35, 54, and 6 months of mouse testis in our Affymetrix chip analysis. By combination with the RT-PCR analysis of mouse Tpap, we observed mouse Tpap began to express at the age of day 18 in mouse. Tpap is an age-dependent gene in mouse testis.The expression of Tpap corresponds to the appearance of spermatids of mice and indicates that Tpap may have an important role in male mammalian spermatogenesis.
4.The expression and significance of circulating microRNA of rats with hypobaric hypoxia-induced pulmonary hypertension
Jiaoyang XU ; Maling SI ; Wenhui SHI ; Yong FU ; Jiangwei LIU ; Jin ZHOU ; Wuzhong YU ; Junhao GUI
Journal of Xi'an Jiaotong University(Medical Sciences) 2016;37(4):556-559
Objective To investigate the expression of circulating microRNA (miRNA) of rats with hypobaric hypoxia‐induced pulmonary hypertension (HPH) .Methods Commercial rat miRNA microarray was employed to detect and analyze the circulating miRNA profile in the serum samples of Sprague‐Dawley rats with hypobaric hypoxia‐induced HPH and controls .Furthermore ,differentially expressed candidate circulating miRNAs between HPH and control groups were validated by Real‐time quantitative PCR based on the case‐control study ,and receiver operating characteristic curve (ROC ) analysis was used to test the performance of four differentially expressed circulating miRNAs in discriminating HPH and control groups .Results Compared with those in the control group ,13 upregulated miRNAs and 10 downregulated miRNAs were identified in hypobaric hypoxia‐induced HPH rats by using miRNA microarray . And differentially expressed miR‐451 , miR‐505 , let‐7d and miR‐214 were validated by using RT‐PCR .ROC analysis showed that the area under the curve of miR‐451 ,miR‐505 and let‐7d was 0 .979 ,0 .938 and 0 .993 in discriminating HPH and control groups ,respectively .Conclusion The aberrant expression of circulating miR‐451 ,miR‐505 and let‐7d in serum may be correlated with the pathogenesis of HPH .
5.Influence of affectivity trait on relationships between occupational stress and subjective physical health and job satisfaction.
Shan-fa YU ; Wen-hui ZHOU ; Gui-zhen GU
Chinese Journal of Industrial Hygiene and Occupational Diseases 2008;26(9):514-517
OBJECTIVETo explore the influence of affectivity trait on relationships between occupational stress and subjective physical health and job satisfaction.
METHODSSelf-reports for psychosocial work conditions and health and well-being in a sample of 878 workers from a thermal power plant in China were conducted using the job demand-control model, the effort-reward imbalance model, job satisfaction, depression symptom, and physical health complaints questionnaires. Logistic regression analysis was used by controlling for age, sex, and educational level.
RESULTSNegative and positive affectivity were correlated with occupational stressors or strains (P<0.01). In the most equation of job dissatisfaction, psychosomatic complaints and depressive symptoms as dependent variables respectively, odds ratios decreased greatly when negative affectivity or positive affectivity was controlled; Odds ratios decreased greater when negative affectivity and positive affectivity were controlled simultaneously. Furthermore in some equation no variable entered.
CONCLUSIONAffectivity trait has effect on relationships between occupational stress and subjective physical health and job satisfaction. According to the outcome category of study, the possible confounding role of affectivity should be controlled in the study of the relationships between occupational stress and health self-reports.
Adolescent ; Adult ; Affect ; Burnout, Professional ; Female ; Health Status ; Humans ; Job Satisfaction ; Logistic Models ; Male ; Middle Aged ; Occupational Health ; Surveys and Questionnaires ; Young Adult
6.Histological findings and its influencing factors of HBeAg-negative chronic hepatitis B virus-infected patients with persistently normal alanine aminotransferase levels
Yanhua YANG ; Qing XIE ; Honglian GUI ; Huijuan ZHOU ; Hui WANG ; Simin GUO ; Cuicui SHI ; Wei CAI ; Hong YU ; Qing GUO
Chinese Journal of Infectious Diseases 2008;26(12):719-724
Objective To identify the histological features as well as factors influencing the course of HBeAg-negative chronic hepatitis B virus ( HBV)-infected patients with persistently normal alanine amino-transferase (ALT) levels (PNAL). Methods Ninety-eight HBeAg-negative chronic HBV-infected patients with PNAL who underwent percutaneous liver biopsy were recruited from October 2003 to March 2008. The ALT level, HBV markers, HBV DNA level and liver histological changes were detected. Comparison of means was done by t test and single factor analysis of variance. Nonparametric statistics was done by Marm-Whitey U test and Kruskal-Wallis test. Analysis of independent risk factor was done using Logistic model. The dianostic value of ALT level to significant liver histological changes was evaluated by receiver performance curve. Results Twenty-two point four percent and 17.3% of subjects had the histological activity index (HAI)≥4and fibrosis (F) score≥3 respectively. Subgroup analysis showed that subjects with ALT>0.50 × upper limit of normal (ULN) had a significantly higher rate of HAI≥4 and F score≥3 than those with ALT≤0.50×ULN (HAI≥4:36.4% vs 11.1%, χ2 =8.881, P=0.003;F score≥3:27.3% vs 9.3%, χ2 =5.487, P= 0.019, respectively), and older subjects (more than 45 years old) had a higher proportion of HAI ≥4 than the younger (33.3% vs 13.4%, χ2 =4.923, P=0.027). Multivariate Logistic regression analysis revealed that a decade increase in age was the independent predictor of HAI≥4 (OR=2.410, P=0.023).Receive operating characteristic (ROC) curve showed that 87.0% and 90.7% of subjects with ALT<0.50× ULN had histological changes of HAI<4 and F score<3 respectively. The proportions of HAI≥4 and F score≥3 in subjects with HBV DNA<1×104 copy/mL were 14.9% and 12.8%, respectively. Conclusions Significant histological changes may be present in part of the subjects with persistently normal ALT and different HBV DNA levels, so that liver biopsy is very important, especially in those with age >45 years.Half time the ULN may serve as an appropriate cutoff value of normal ALT level for managing Chinese HBeAg-negative chronic HBV-int'ected patients.
7.Basal levels for gonadotropins in 318 children from birth to 6 years of age.
Zuang-jian XU ; Yu HU ; Gui-zhen WANG ; Yao-ming WANG ; Li-fen MAO ; Jing-yue ZHOU ; Xu-yan CHEN
Chinese Journal of Pediatrics 2003;41(2):148-148
Child
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Child, Preschool
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Female
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Follicle Stimulating Hormone
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blood
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Gonadotropins
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blood
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Humans
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Infant
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Infant, Newborn
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Male
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Sex Factors
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Time Factors
8.Inhibition of osthole for resorption of rats femur tissue in vitro.
Jian ZHOU ; Xue-mei REN ; Xiao-ni MA ; Yu-hai GAO ; Li-juan YAN ; Wen-gui SHI ; Ke-ming CHEN
China Journal of Orthopaedics and Traumatology 2015;28(9):832-837
OBJECTIVETo investigate osthole effect on femoral tissue resorption activity of rat in vitro.
METHODSSix SD rats weighted (80 ± 5) g were used to isolate and culture femoral tissue (diaphyses and metaphysis) in vitro. The cultured tissue were devided into control group, estradiol group and osthole group. The femoral tissue was treated with final concentration of 1 x 10(-5) mol/L osthole and 1 x 10(-8) mol/L estradiol culture in vitro at 48 hours after cultured. Tartrate-resistant acid phosphatase (StrACP) activity, glucose and Lactic acid content, StrACP, MCSF (Macrophage colony stimulating factor) and CTSK (Cathepsin K) mRNA was detected by Real-Time RT-PCR were detected.
RESULTSConcetration of Alkaline phosphatase activity were 2226 and 2498 in 1 x 10(-5) mol/L osthole and 1 x 10(-8) mol/L estradiol respectively. As compared with control group, the activity of StrACP of 1 x 10(-5) mol/L osthole and 1 x 10(-8) mol/L estradiol were inhibited at 6, 9, 12 days (P < 0.05); under treatment of in l x 10(-5) mol/L osthole, the content of Lactic acid were increased and the content of glucose were decreased at 3, 6, 9 days (P < 0.05); StrACP, MCSF and CTSK mRNA expression level were inhibited at 6, 9 days (P < 0.05).
CONCLUSIONOsthole can inhibit bone resorption and raise the level of nutrition metabolism of femurs tissue.
Acid Phosphatase ; metabolism ; Animals ; Bone Resorption ; prevention & control ; Coumarins ; pharmacology ; Estradiol ; pharmacology ; Femur ; drug effects ; Glucose ; analysis ; Lactic Acid ; analysis ; Male ; Rats ; Rats, Sprague-Dawley
9.FGF-21 protects H9c2 cardiomyoblasts against hydrogen peroxide-induced oxidative stress injury.
Miao-Miao HAN ; Wen-Fei WANG ; Ming-Yao LIU ; De-Shan LI ; Bing ZHOU ; Yin-Hang YU ; Gui-Ping REN
Acta Pharmaceutica Sinica 2014;49(4):470-475
Fibroblast growth factor-21 (FGF-21) is an important metabolism regulator, however, whether FGF-21 has effects on cardiovascular remains unclear. In this study, H2O2-induced injury in H9c2 cells was used as a cell model, the anti-apoptosis potential and mechanism of FGF-21 against oxidative injury were evaluated by MTT assay, flow cytometry assay and real-time PCR. The results showed that FGF-21 could increase the cell survival of H2O2-induced injury in H9c2 cells and prevent H9c2 cells from oxidative stress-induced apoptosis. Furthermore, FGF-21 can elevate SOD activity and regulate Bcl-2/Bax expression in H9c2 cells. The results suggest that FGF-21 have protective effect against the H2O2-induced apoptosis in H9c2 cells.
Animals
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Apoptosis
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drug effects
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Cell Proliferation
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drug effects
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Cells, Cultured
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Fibroblast Growth Factors
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pharmacology
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Hydrogen Peroxide
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toxicity
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Malondialdehyde
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metabolism
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Myocytes, Cardiac
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cytology
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drug effects
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metabolism
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Oxidative Stress
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drug effects
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Protective Agents
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pharmacology
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Proto-Oncogene Proteins c-bcl-2
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genetics
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metabolism
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RNA, Messenger
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metabolism
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Rats
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Reactive Oxygen Species
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metabolism
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Superoxide Dismutase
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metabolism
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bcl-2-Associated X Protein
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genetics
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metabolism
10.Apelin protects against cardiomyocyte apoptosis induced by glucose deprivation.
Zhi ZHANG ; Bo YU ; Gui-zhou TAO
Chinese Medical Journal 2009;122(19):2360-2365
BACKGROUNDApoptosis is a major cause of ischemic heart dysfunction. Apelin, the endogenous ligand for the G-protein-coupled APJ receptor, has been reported to exert cardioprotective effects during myocardial injury. The aim of this study was to investigate the effects of apelin on apoptosis of rat cardiomyocytes induced by glucose deprivation (GD) and study the related signaling pathway.
METHODSApelin and APJ mRNA expression were determined by RT-PCR in neonatal rat cardiomyocytes during different durations of GD. Cardiomyocyte apoptosis was detected by annexin V-FITC/propidium iodide (PI) staining after GD for 12 hours with or without apelin-13 (10 and 100 nmol/L) pretreatment. Protein levels of Akt and the mammalian target of rapamycin (mTOR) as well as cell apoptosis were detected in the presence or absence of LY294002 (a phosphatidylinositol 3-kinases (PI3K) inhibitor) or rapamycin (a mTOR inhibitor).
RESULTSApelin mRNA expression was up-regulated when cardiomyocytes were exposed to GD for 6, 12, 18, and 24 hours compared with the base level (P > 0.05, P < 0.01, P < 0.01, P < 0.01). However, when cardiomyocytes were exposed to GD for up to 36 hours, apelin mRNA expression was 17% lower than the base level (P < 0.05). APJ mRNA expression paralleled that of apelin. Apelin-13 pretreatment at 100 nmol/L significantly inhibited GD-induced cardiomyocyte apoptosis (P < 0.05) and increased Akt and mTOR phosphorylation (P < 0.01, P < 0.01). At the same time apelin-13 (100 nmol/L) up-regulated Bcl-2 protein expression and down-regulated Bax and cleaved caspase-3 expression (P < 0.01, P < 0.05, P < 0.05). The anti-apoptotic effect of apelin-13 was blocked by LY294002 (P < 0.01) but not by rapamycin.
CONCLUSIONSThe endogenous apelin-APJ system is compensatorily up-regulated and ultimately down-regulated following sustained myocardial ischemia. Apelin protects against ischemic cardiomyocyte apoptosis via activation of the PI3K/Akt pathway.
Animals ; Apelin ; Apelin Receptors ; Apoptosis ; Carrier Proteins ; genetics ; physiology ; Caspase 3 ; analysis ; Cell Survival ; Cells, Cultured ; Glucose ; deficiency ; Intercellular Signaling Peptides and Proteins ; Myocytes, Cardiac ; physiology ; Phosphatidylinositol 3-Kinases ; physiology ; Proto-Oncogene Proteins c-akt ; physiology ; Proto-Oncogene Proteins c-bcl-2 ; analysis ; RNA, Messenger ; analysis ; Rats ; Rats, Sprague-Dawley ; Receptors, G-Protein-Coupled ; physiology ; Signal Transduction ; bcl-2-Associated X Protein ; analysis