1.Clinical value of 34?E12 immunostaining for the differential dianosis of prostatic carcinoma
Danhua SHEN ; Dajun ZHONG ; Youzhi YU
Chinese Journal of Urology 2001;0(04):-
ObjectiveTo investigate the clinicopathological features of the needle biopsies of prostatic lesions and to evaluate the value of 34?E12 immunostaining for the diagnosis of prostatic carcinoma.MethodsThe clinical data,levels of serum PSA and HE slides of 103 cases of the needle biopsies of prostatic lesions were reviewed.Immunohistochemical stains for 34?E12 were performed.ResultsThe morphological features revealed 36 cases of benign prostatic hyperplasia(BPH),9 cases of low grade prostastic intraepithelial neoplasia (PIN I)and 34 cases of prostatic carcinoma(PC),24 cases of high grade PIN(PINⅡ and Ⅲ) have been suspected of carcinoma.With immunohistochemical stain,all cases of BPH were strongly reactive for 34?E12 while 32 cases of prostatic carcinoma were negative;14 of 24 cases of high grade PIN were negative for 34?E12 and could be diagnosed as PC.The rest 10 of high grade PIN were interruptedly positive in the basal layer for 34?E12.In the 30 cases of prostastic carcinoma,serum PSA levels of 25 cases was over 10 ng/ml and over 50 ng/ml in 15.ConclusionsThe pathologic diagnosis should based on gland architectures and cytologic features.34?E12 negative expression and elevation of serum PSA are also the important criteria for the diagnosis of prostatic carcinoma.
2.Application of Mitomycin C during Operation of Pterygium for Decreasing Recurrence
yu, CHENG ; yi-sheng, ZHONG ; xi, SHEN
Journal of Shanghai Jiaotong University(Medical Science) 2006;0(11):-
0.05).The recurrence of pterygium was related to the age.If the age increased five years,the risk of recurrence decreased 18.1%. Conclusion The application of MMC(during) the operation could decrease the recurrence rate of pterygium.The recurrence rate of pterygium was not related to the time of application of 0.02% MMC,and detainment for 3 min was enough during the operation.
4.Two cases with recurrent enlarged parotid gland.
Shen-xia YUAN ; Yu-zhong ZHAO ; Rong-fen LIU
Chinese Journal of Pediatrics 2008;46(11):874-875
Adolescent
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Child
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Female
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Humans
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Parotid Diseases
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pathology
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Parotid Gland
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pathology
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Recurrence
5.Practice and thinking of standardized training for ophthalmology residents
Xi SHEN ; Qin JIAO ; Yu CHENG ; Bing XIE ; Yisheng ZHONG
Chinese Journal of Medical Education Research 2013;(7):732-735
As one of the first resident standardized training bases, department of ophthalmology of Shanghai Ruijin Hospital participated in this reform process from 2010. Relevant rules and regulations (training management system , training scheme implementation system and evaluation system ) were strictly obeyed. When new problems emerged, under the guidance of department in charge, a series of regimens were formulated and improved gradually by Ophthalmology Professional Committee of Shanghai Resident Standardized Training Department. Based on reviewing and summarizing the work in the last 3 years, some thoughts and suggestions on the resident standardized training in future were put forward ,in-cluding how to better solve the“heavily used, lightly cultured” problem, the“disregarding medical ethics establishment”problem, the“disregarding assessment of teachers”problem and the“disregard-ing obtaining employment”problem.
6.Preparation and evaluation of sustained-release polylactic acid microspheres of bupivacaine in vitro
Yuan YU ; Shen GAO ; Yanqiang ZHONG ; Xueying DING
Academic Journal of Second Military Medical University 1982;0(01):-
Objective: To prepare sustained-release polylactic acid microspheres containing bupivacaine (BUP-PLA-MS) and to measure its dissolution in vitro. Methods: BUP-PLA-MS was prepared with polylactic acid (PLA) as carriers using the water-in-oil-in-water (W/O/W) emulsion solvent evaporation method. The powder particle characteristics of BUP-PLA-MS were evaluated comprehensively, and its dissolution characteristics in vitro were studied. Results: It was indicated that bupivacaine formed molecular dispersion within PLA matrix by differential thermal analysis(DTA). The in vitro release behavior of bupivacaine could be best described by Higuchi equation, with t 1/2 =22.76 h. Conclusion: Release of bupivacaine from microspheres is sustained in vitro.
7.Purification of Recombinant Fusion Protein Staphylokinase-Hirudin Expressed by Escherichia coli and Analysis of its Dimer
Gen-Shen ZHONG ; Ai-Ping YU ; Ji-De JIN ; Zhong-Hua JIANG ; Zu-Ze WU ;
China Biotechnology 2006;0(02):-
The recombinant fusion protein staphylokinase-hirudin(rSFH) was purified from the high density-fermented engineered E.coli by means of ion-exchange chromatography (IEC) and gel filtration (GF). The purity of rSFH reached to more than 98% determined by RP-HPLC and SDS-PAGE, and the yield was up to 0.7g per liter of fermentation broth. The analysis of homologous dimmer of rSFH appeared during the purification and calculation of the surface hydrophobic area had been carried out by means of hydrophobic chromatography and MALD-TOF. The influence of sodium chloride and temperature on the behavior of rSFH reversible dimerization was analyzed by high performance sized- exclusive chromatography(HPSEC). It is concluded that the hydrophobic interaction played an important role in the reversible dimerization of rSFH.
8.Construction of RNAi vectors for SmNAC1 transcription factors of Salvia miltiorrhiza using Gateway cloning technology.
Rong ZHAO ; Qi-Xian RONG ; Yu-Zhong LIU ; Ye SHEN ; Lu-Qi HUANG
China Journal of Chinese Materia Medica 2014;39(9):1569-1573
NAC transcription factors involved in plant growth and development, as well as responses to biotic and abiotic stress. RNAi Vectors for SmNAC transcription factors of Salvia miltiorrhiza was constructed by using Gateway cloning technology, in order to further study the function of SmNAC1 transcription factor. According to Gateway cloning technology, the specific fragments of SmNAC1 containing attB adapter was amplified by PCR using ultra-fideling phusion polymerase of NEB. By the BP recombination reaction, the PCR product containing attB was transferred to an donor vector (pENTR/SD/D-TOPO). Finally, SmNACi specific gene was cloned into pK7GWIWG2D plant expression vectors by LR recombination reaction. Experimental results showed that Gateway cloning technology provide a rapid and highly efficient way to clone the interested gene.
Cloning, Molecular
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methods
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Genetic Vectors
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genetics
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Plant Proteins
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genetics
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Polymerase Chain Reaction
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RNA Interference
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Reproducibility of Results
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Salvia miltiorrhiza
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genetics
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Transcription Factors
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genetics
9.Pain-alleviating effect of bupivacaine polylactic acid microspheres in rabbits
Qiang FU ; Xinhua WANG ; Zui ZOU ; Yuan YU ; Shen GAO ; Yanqiang ZHONG ; Hong ZHANG
Chinese Journal of Tissue Engineering Research 2006;10(25):181-183
BACKGROUND: Bupivacaine is widely used to alleviate post-operation pain and cure acute and chronic pain caused by inflammation or cancer.Its analgesic time cannot meet the request that drug is released slowly to prolong the analgesic time in clinic.OBJECTIVE: To detect the alleviating effect of bupivacaine polylactic acid microspheres taking high molecular polymer-polylactic acid as vector in rabbits with high performance liquid chromatograph (HPLC) and traditional skin test method.DESIGN: A completely randomized controlled animal experimental study.SETTING: School of Pharmacy, Second Military Medical University of Chinese PLAMATERIALS: Sixteen New Zealand rabbits, weighing (2.58±0.17)kg were used in this experiment.INTERVENTIONS: The experiment was carried out at the Department of pharmaceutics, School of Pharmacy, Second Military Medical University of Chinese PLA between September and November 2002. ① Animal models were established according to traditional skin test method. ② Totally 16 New Zealand rabbits were randomly divided into 2 groups: Group A and Group B, with 8 in each one. 5 mg/kg bupivacaine parenteral solution was injected subcutaneously in Group A, 5 mg/kg bupivacaine polylactic acid microspheres were implanted between subcutaneous tissue and sarcolemma in Group B. We took 1.5 mL blood from ear border vein at 5, 10, 20, 30,45 minutes, 1, 2, 3, 4, 6, 8, 12 and 24 hours after administration of bupivacaine parenteral solution respectively in Group A and another 1.5 mL at 0.5, 1, 2, 3, 4, 5, 6, 8, 12, 24, 3 6, 48 and 60 hours after admistration of bupivacaine microsphere powder for index detection. ③ HPLC method was used to detect the concentration and releasing effect of bupivacaine in blood serum.MAIN OUTCOME MEASURES: Concentration change of bupivacaine in blood serum and efficacy diameter of local anesthetic.RESULTS:All the 16 rabbits entered the stage of result analysis. ①Change of bupivacaine concentration: Plasma bupivacaine concentration in Group A reached the peaked quickly after subutaneous injection with the high concentration of 2.466 4 mg/L, then declined quickly. Plasma bupivacaine concentration in Group B was relative stable, reached a peak much slowly after subcutaneous implantation, with peak concentration of 0.778 1 mg/L, and the plasma bupivacaine concentration maintained a relative low level, the mean retention time was obviously prolonged (P < 0.05).② Alleviating effect of bupivacaine: The analgesic time was significantly longer in the bupivacaine microsphere group than in the bupivacaine parenteral solution group (P < 0.05).CONCLUSION:Bupivacaine polylactic acid microspheres have sustained release effects in rabbits.
10.Influence of UGT1A9 genetic polymorphisms on mycophenolic acid exposure in Chinese kidney transplant recipients
Zhihong LIU ; Bing SHEN ; Hua GONG ; Zhong WANG ; Qinghua WANG ; Jie ZHU ; Yu FAN
Chinese Journal of Organ Transplantation 2010;31(10):607-610
Objective To analyze the relationship between the genetic polymorphisms of uridinediphosphate glucuronosyltransferase 1A9 (UGT1A9) and mycophenolic acid (MPA)pharmacokinetics in Chinese kidney recipients.Methods Gene mutations (C-440T/T-331C,C-2152T,T-275A,T98C) were detected in 196 recipients by PCR-LDR.On the 28th day after transplantation,the plasma samples which were obtained at the time points of predose,0.5 h and 2 h after administration were measured by an immunoassay method (Emit Mycophenolic Acid Assay,Dade Behring).MPA-AUC0-12 was calculated based on these three data.Correlation between single nucleotide polymorphisms (SNPs) and MPA pharmacokinetics was analyzed.Results C-2152T,T-275A and T98C genotypes of UGT1A9 were not found in 196 recipients.The frequency of C-440T/T-331C gene mutation was 14.29% (28/196).The mean value of MPA-AUC0-12 was 40.6±11.8 wild genotype,respectively (P>0.05).Conclusion C-2152T,T-275A and T98C genotypes of UGT1A9 are scarce in Chinese kidney recipients.In this study,there is no distinct relationship between -440/-331 SNPs and MPA pharmacokinetics in Chinese allograft recipients.