The main results of the article are as follows:GS(10ug/ml,25ug/ml,50ug/ml)could augment NK activity of murine spleen cells in vitro(p

The initial study of the tumour DNA fingerprints using MYO minisatellite DNA probe wascarried out,and then,by means of DNA recombinant techniques,the fragment of MYO min-isatellite DNA probe obtained from plasmid pUC19-MYO was inserted into plasmid pGEM-4Z containing RNA polymerase promotor,thus a sub-clone refferred as pGEM-4Z-MYOwas constucted.That made an offer of the conditions of preparing RNA probe in order to in-cerase the sensitivities of DNA fingerprinting and laid a foundation for raised the efficiency of de-tecting the polymorphism of the minisatellite DNA.

A description of the implementation strategy and methodology of clinical medicine as a discipline in terms of building clinical technology features, making scientific research centering on clinical questions, building technical and academic branding, and building discipline bases.Authors also emphasized human resource cultivation, and held that the clinical discipline development should be carried out by means of organization and culture management for the discipline.

Arterio-Arterial Fistula ; complications ; Coronary Artery Disease ; complications ; Coronary Stenosis ; complications ; Female ; Humans ; Middle Aged

Objective:To investigate the non-thymoma thymic morphology in patients with Myasthenia Gravis(MG), and provide the theoretical basis for extended thymectomy.Methods:From September 2008 to March 2018, extended thymectomy had been performed for 150 patients with MG in our department. In order to achieve maximal benefit, combined thoracoscopic and mediastinoscopic approach had been adopted to completely remove both thymus and ectopic thymic tissue in the neck and the anterior mediastinum. Cervical and mediastinal tissue including fat from the thyroid isthmus to the diaphragm was swept away from above the phrenic nerve. The regions containing mediastinal fat were divided into 12 stations.Results:There was no mortality. Pathology reports revealed 126(84.0%) patients with MG had hyperplastic thymus, 17(11.3%)cases with involuted thymus and 7 (4.7%)cases with normal thymus. 98 (65.3%)cases had more than two superior horns, and 59(39.3%) patients had ectopic thymic tissue in the mediastinum and in the neck. Positive rates of ectopic thymic tissue in the 12 cervical-mediastinal fat stations were 7.8%, 10.0%, 8.8%, 3.8%, 3.0%, 2.5%, 0, 0, 0.7%, 13.3%, 12.3% and 9.1%, respectively. After a follow-up period of 2-12 years, the rate of complete stable remission was 44.7%(59/132), and the effective rate was 89.4%(118/132).Conclusion:It is very meaningful to study non-thymoma thymic morphology in patients with MG in order to guide the performance of extended thymectomy and improve its outcome. For the purpose of minimally invasion, one side of mediastinal pleural should be carefully protected.

Objective To investigate the effect and mechanism of Imatinib mesilate (Imatinib) on intimal hyperplasia of rabbit carotid arteries after balloon injury. Methods Thirty adult Newzealand rabbits were randomly divided into three groups:group A, B and C. Their right carotid arteries were injuried then administered with 0, 25 or 50 mg/kg of Imatinib dai?ly for 14 consecutive days when the rabbits were sacrificed. The carotid arteries were harvested and sectioned for HE-stain?ing and immunohistochemisty staining. Real-Time PCR was used to examine transcription levels of PDGF-B and PDGFR-βmRNA. The plasma level of PDGF-BB was assayed by ELISA. Results Arterial intimal hyperplasia and stenosis following balloon injury were seen in three groups. Thickness and area of neointima, ratio of thickness of intima to media, ratio of area of intima to media and mRNA level of PDGF-β are all higher in group A than those in group B than those in group C (P<0.01). By contrast, the mRNA transcription level of PDGFR-β increased significantly in group C than that in group A (1.236±0.356 vs 0.708±0.372;t=2.91;P<0.01). Plasma level of PDGF-BB increased in all three groups after balloon injury than that in the baseline (P<0.01). The transcription level of PDGF-BB is higher in group A than that in group B and in group C (ng/L:23.464±3.542, 19.504±2.454, 16.588±1.207, F=17.322, P<0.05). There was no difference between group B and C. There was positive correlation between mRNA transcription level of PDGF-B and plasma level of PDGF-BB ( r=0.806, P<0.01). Conclusion Vascular injury can cause intimal hyperplasia and increased PDGF-B mRNA transcription. Imatinib mesilate could inhibit the intimal hyperplasia through down regulating PDGF-B mRNA transcription.

Objective To investigate the effect of jejunal infusion of amino acids on secretion of gastrointestinal hormone in healthy dogs.Methods Six healthy adult dogs were treated with jejunal fistulas and femoral vein intubation.Twenty-four hours after the operation,solution of 8 different amino acid monomers (experimental group) or normal saline (control group) were infused into the jejunum of the dogs every 24hours.The levels of cholecystokinin (CCK),motilin,and gastrin in the peripheral plasma were measured using radioimmunoassay at the start of infusion (0 minute),and 30,60,90,and 120 minutes after infusion.Results Compared with the control group,the serum CCK level in the phenylalanine group was significantly higher 30 and 60 minutes after infusion [(1.25 ±0.19) ng/L vs.(0.66 ±0.14) ng/L,(1.23 ±0.12) ng/L vs.(0.80 ± 0.03) ng/L,both P ＜ 0.01],while that in the tryptophan group was significantly higher 30 minutes after infusion [(1.08 ±0.26) ng/L vs.(0.66 ±0.14) rig/L,P ＜0.01].The other measurement results showed no statistically significant differences.Conclusions Jejunal infusion of phenylalanine or tryptophan may stimulate the secretion of gastrointestinal hormone to some extent.Aromatic amino acids (phenylalanine and tryptophan) is more potent in triggering the release of CCK than aliphatic (leucine,isoleucine,and methionine) and charged amino acids (aspartic acid,arginine,and glutamate).The mechanism may be related to the properties of the amino acids.

Objective:To observe the effect of diammonium glycyrrhizinate injection on bronchus,lung tissue and amount of eosinophils(EOS)in rats with asthma and to discuss the mechanism of diammonium glycyrrhizinate intervention on airway inflammation in asthma.Methods:To divide 60 male SD rats into 6 groups as following:normal control group,model group,dexameth group,high dose of diammonium glycyrrhizinate group,middle dose of diammonium glycyrrhizinate group and low dose of diammonium glycyrrhizinate group(n=10).After setting up the model of rats with asthma,we detect the EOS in both rats'blood serum and bronchoalveolar lavage and observe the pathological change in bronchus and lung tissue in rats of each group.Results:Not only the amount of EOS of rats in model group is bigger than that in normal control group(P

One hundred and ten college students, 66 males and 44 females, were studied for the evaluation of vitamin C status by use of the ratio of reduced vitamin C to creatinine in a random urine sample. During the experimental period, the dietary source of creatinine was strictly abstained. After voiding in the morning, the volunteers were weighed and their urine were collected at the end of 1st, 2nd, 4th, 8th, 12th and 24th hr respectively. The contents of reduced vitamin C and creatinine in each collected urine sample were analysed, and then the total amounts in 24 hrs were calculated.The experimental results showed:1. The distribution of the creatinine excretion in 24 hrs was nearly constant.2. The percentage of reduced vitamin C excretion per hour to the total 24 hrs excretion was similar in subjects of different vitamin C status and was lowest in 12 hrs of night urine. A curvilinear regression was given for the percentage of reduced vitamin C excretion in relation to the time.3. The ratio of reduced vitamin C (as mg) to creatinine (as gm) and the ratio of reduced vitamin C to creatinine (as mg) per body weight (as kg) were calculated from their contents in 12 hrs urine in those who exc- reted reduced vitamin C in urine less than 80 mg in 24 hrs. The relationship between these two ratios and the excretions of reduced vitamin C in 24 hrs urine was studied and four equations of curvilinear regressions were given.4. According to these equations, the correlated values of vitamin C/ crcatinine and vitamin C/creatinine/body weight in random urine samples in respect of four different grades of vitamin C excretion of 24 hrs urine were recommended.It was shown to be a new simplified method for evaluating the nutritional status of vitamin C in human body. The practical aspect of its application was discussed.

Objective To understand the level of resilience and quality of life of burn patients during convalescence,to provide theoretical guidance for burn patients' psychological crisis prevention and intervention.Methods 255 cases of burn patients during convalescence were interviewed with general status questionnaire,CD-RISC,and burn specific health scale(BSHS).Results The resilience score of burn patients was at the middle level,the level of quality of life was at the lower-middle-level,significant positive correlation was found between the level of resilience and quality of life.Resilience level had a greater impact on the level of mental health in burn patients.The factors of physical strength and tenacity of resilience scale were positively correlated with mental health.Conclusions Intervention should be given to help people improve resilience,in order to reach the effect of potential development and prevention of behavior dysfunction,so that help them engender positive psychological adjustment.