1.Application of wireless transfer technology to medical instrument development
Xuefei YU ; Guangwen LU ; Zhe LI
Chinese Medical Equipment Journal 1989;0(02):-
In many medical instrumentation systems, there exists a long distance between manipulation platform and host unit. Wireless data transferring between the platform and the host could be a feasible way to set parameters. This paper introduces wireless transmitter and receiver circuit based on encoder chip PT2262 and decoder chip PT22720, and presents some typical applications in medical instrument development.
2.Relationship between microsatellite instability and deficiency of DNA mismatch repair system in lung carcinoma
Man LI ; Zhe YU ; Yinghua LI ; Jing ZHANG
Cancer Research and Clinic 2011;23(4):227-229
Objectiye To investigate the relationship between microsatellite instability (MSI) and mismatch repair gene (MMR) in lung carcinoma,and to analyze its action in lung carcinogenesis.Methods DNA was extracted from 50 cases of lung carcinoma and adjacent normal tissue.SSCP was used to detect MSI.Immunohistochemistry was performed to measure the expression of hMLH1 and hMSH2 protein in lung carcinoma.Results Of the 50 cases of lung carcinoma,14 with high MSI,21 with low MSI,and 15 with microsatellite stability,0 with MSI in normal group (P = 0.000).Lack of hMLH1 and hMLH2 staining was found in 37 of 50 (74 %) and 16 of 50 (32 %) lung carcinoma tissues with MSI,respectively; but both of hMLH1 and hMSH2 positive staining was found in the lung carcinoma tissues with MSS.Conclusion MSI appears to be associated with lung carcinogenesis,and inactivation of either hMLH1 or hMSH2 may be responsible for MSI,suggesting that MSI may become one of diagnosis indexes of lung carcinoma.
3.Preparation and adhesive characterization of colon adhesive pellets of sodium 4-amino salicylate
Lian YU ; Zhe ZHANG ; Chengchong LI ; Chuanmei ZHANG ; Aichen LI
Journal of China Pharmaceutical University 2009;40(6):510-513
Aim: To develop a new preparation consisting of colon adhesive pellets of sodium 4-amino salicylate for the treatment of ulcerative colitis, and to characterize the adhesive of the prepared pellets. Methods: Centrifu-gation-granulation was used to prepare sodium 4-amino salicylate pellets into which HPMC K4M and Carbomer 934P as matrix were incorporated. The pellets were coated with Surlease~((R)), and filled into enteric-coated cap-sules. The release of sodium 4-amino salicylate from the pellets was evaluated in the pH 7. 4 buffer. The adhesive property of the pellets was characterized by evaluating the transit rate of the pellets in the rat intestine in vivo, and by determing the remaining( %) of the pellets in the isolated rat colonic segment in vitro. The adhesive of the pel-lets in vivo was also verified by X-rays. Results: It was shown that the resultant pellets of round shape, good uni-formity in size, and favorable rigidity possessed the colon-adhesive and sustained-release properties. Conclusion:The simple, economical and practical approach was sucessfully utilized in the preparation of sodium 4-amino sa-licylate pellets with the remarked colonic adhesion and targeting.
4.Design of Electric Stimulator of Cell Culture
Shuyan GUO ; Xuefei YU ; Lei ZHENG ; Weiqi LI ; Zhe LI
Chinese Medical Equipment Journal 1993;0(05):-
Objective To design an instrument that can provide a series of pulse to stimulate cell by means of external electric field, the structure and function of organism can retrieve. Methods AVR MCU, produced by America ATMEL Co., is used as the core of the system. The program has been developed to adjust three pulse's parameters, including amplitude, frequency and pulse duration. The cooperation between DAC and OP completes the transformation from monopole pulse to bipolar pulse. The booster PB50 amplifies the current and voltage of the output. Results The Stimulator can provide bipolar pulse, amplitude: up to ?40V, frequency: 0.01Hz -10Hz, pulse duration: 0.4ms -24ms. Conclusion Cooperating with special electrode board, the instrument can provide effective electric field for cell simulating. At present the instrument has been used in the research of the endothelial progenitor cell.
5.Rhizopus arrihizus swollen spores induce mice dendritic cells to Th1 and Th17 differentiation
Limin YAO ; Shuzhen ZHANG ; Zhe WAN ; Ruoyu LI ; Jin YU
Chinese Journal of Microbiology and Immunology 2017;37(1):14-21
Objective To study the mechanism of adaptive immunity against Rhizopus arrihizus (R. arrihizus) infections. Methods Bone marrow derived dendritic cells (BMDCs) were separated from C57BL/6 mice and Card9-/- mice and then were cultured in vitro. Resting spores and swollen spores of R. arrihizus were in vitro co-cultured with BMDCs with or without Syk inhibition. Secretion of cytokines ( IL-23, IL-1βand IL-12) was analyzed by ELISA after 24 hours of culture. Na?ve T cells derived from C57BL/6 mice were in vitro co-cultured with spore-stimulated BMDCs for four days. Levels of IL-17A and IFN-γ in supernatants of cell culture were analyzed by ELISA. Flow cytometry was performed to analyze T cell differ-entiation. Confocal microscopy was used to observe the images of stained β-glucan on the surface of resting and swollen spores. Swollen spores were co-cultured with Dectin-1, Dectin-2, TLR2 and mannose receptor ( MMR) , and the binding results were analyzed by flow cytometry. Results Swollen spores but resting spores could induce the maturation of BMDCs and promote the secretion of cytokines (IL-23, IL-1βand IL-12). Co-culturing T cells with swollen spore-stimulated BMDCs enhanced their differentiation to Th17 and Th1. In addition, swollen spores promoted the secretion of Th1-related cytokine ( IFN-γ) and Th17-related cytokine (IL-17A). Adding Syk inhibitor to Card9-/-BMDCs or wild type BMDCs significantly inhibited the secretion of cytokines and T cell differentiation, especially in the Card9-/- group. β-glucan was overserved on the surface of swollen spores, but not on resting spores. On the surface of swollen spores existed pathogen associated molecular patterns ( PAMPs) that could bind with Dectin-1 and TLR2. Conclusion Swollen spores of R. arrihizus could active BMDCs to secrete cytokines of IL-23, IL-1β and IL-12 and trigger T cell responses in vitro. The possible mechanism might be associated with β-glucan exposed on the surface of swollen spores that binds with Dectin-1. The responses between BMDCs and R. arrihizus are Syk-Card9-dependent.
6.Clinical observation on the effect of omeprazole combined with octreotide in treatment of patients with nonvariceal upper gastrointestinal hemorrhage
Wang FANG ; Liu LI ; Hong ZHE ; Yu JIAN
Chinese Journal of Primary Medicine and Pharmacy 2011;18(24):3350-3351
ObjectiveTo explore the clinical effect of omeprazole combined with octreotide in treatment of patients with non-variceal upper gastrointestinal hemorrhage.Methods96 patients with non-variceal upper gastrointestinal hemorrhage were randomly divided into two groups.All the cases were received basic treatment of fluid infusion,transfusion and nutritional support.The control group( n =48) was treated with omeprazole alone,and the treatment group(n =48) was treated with omeprazole and octreotide.The course of treatment was 3 days.The vital signs,24h urine output,the number of cases of rebleeding in 72 h and adverse drug reactions was observed and recorded.ResultsThe total effective rate in treatment group and control group was 91.7% and 72.9%,respectively.The difference between the two groups was statistically significant( x2 =5.79,P <0.05 ).The time of hemostasis and blood transfusion volume in treatment group were significantly less than those in control group(t =7.69,9.91,all P <0.05).The rebleeding rates after 72 hours of hemostasis between the two groups(8.3% vs 25.0% ) was significantly different ( x2 =4.80,P < 0.05 ).In the course of treatment,the side effects weren' t found in both groups.ConclusionOmeprazole combined with octreotide was more effective and safe than omeprazole alone in fast stopping bleeding and reducing rebleeding rate.
8.Development of programming cardiac trigger apparatus and its application to myocardial contrast echocardiography
Zhe LI ; Daogang ZHA ; Guangwen LU ; Xuefei YU ; Hao CHEN
Chinese Medical Equipment Journal 1989;0(04):-
This paper introduces a programming cardiac trigger apparatus used for the study of quantitative measurement of myocardial blood flow with myocardial contrast echocardiography.The design of hardware circuit based on MCU and the scheme of software based on Keil C51 are mainly discussed.With stable and reliable working,this apparatus provides a kind of technical support for the study of quantitative measurement of myocardial blood flow with continuous intravenous injection of sonicated microbubbles.
9.Change Rules of Quantity and Species of Diatoms in Hunhe River in Shenyang
Yu DU ; Zhe ZHOU ; Hongyang CAI ; Pengfei LI ; Junling XIA
Journal of Forensic Medicine 2013;(5):337-339,343
Objection To observe the change rules of quantity and species of diatom sin Hunhe River in Shenyang and to provide technology and scientific evidence for drow ning identification and the location of drow ning in forensic investigation. Methods In 2011, different locations for collecting water sam pleswere chosen in Hunhe River in Shenyang. Water sam pleswere collected and variation of quantity and species of diatom swere observed every month. And variation of dom inant species of diatom swas ob-served every week. Results The quantity, speciesand dom inant species of diatom sin Hunhe River in Shenyang varied with different tim eand locations. The quantity and species of diatom swere lowest from Decem ber to February and gradually increased, reaching peak in May and second peak in October, and then gradually decreased. The dom inant species of diatom s varied significantly adjacent two weeksat same location from April to N ovem ber, but had little changesat different locationsin same week from July to August. Conclusion The change rules of quantity and species of diatom sare com plicated and affected by various factors such as environm ent and hydrology. The change rules of speciesand quantity of diatom s should be considered in forensic investigation of drow ning identification and the lo-cation of drow ning.
10.Study and application on hepatocytes of bioartificial liver support system
Yu LI ; Zhe XU ; Yan ZHANG ; Xuefan BAI
Journal of Medical Postgraduates 2003;0(10):-
The study on bioartificial liver support system was widely accepted in recent years. It's mainly containing material-hepatocytes. This summary is concerning about the research and application of hepatocytes in this field.