1.Role of EGFR mutation status in patients with advanced lung adenocarcinoma treated with pemetrexed
Qiong ZHANG ; Zhe YANG ; Honghai DAI ; Yu WANG ; Junqing HAN
Chinese Journal of Clinical Oncology 2013;(22):1378-1381
Objective:To assess the role of EGFR mutations on pemetrexed response in patients with advanced lung adenocarcino-ma. Method: Forty pulmonary adenocarcinoma patients with evaluable lesions were retrospectively screened .They had been treated with pemetrexed-included chemotherapy and had EGFR gene test results. The evaluation endpoints were overall response rate,disease control rate and progression free survival. Result:No significant statistical difference was seen in overall response rate(ORR) (44.4%VS 31.8%, respectively) and disease control rate(DCR) (88.9%VS 81.8%, respectively ) between EGFR wild group and EGFR muta-tion group, but patients in EGFR wild group had longer progression free survival(PFS) ( 8.9 months VS 5.3 months;P=0.046). Conclu-sion:EGFR mutation status can influence the efficacy of pemetrexed.
2.New dicyclopeptides from Dianthus chinensis.
Jing HAN ; Zhe WANG ; Yu-Qing ZHENG ; Guang-Zhi ZENG ; Wen-Jun HE ; Ning-Hua TAN
Acta Pharmaceutica Sinica 2014;49(5):656-660
One new dicyclopeptide cyclo-(L-N-methyl Glu-L-N-methyl Glu) (1), together with one new natural dicyclopeptide cyclo-(L-methyl Glu ester-L-methyl Glu ester) (2), and two known dicyclopeptides cyclo-(L-methyl Glu ester-L-Glu) (3), and cyclo-(L-Glu-L-Glu) (4), were isolated from the aerial parts of Dianthus chinensis L. Their structures were determined by spectroscopic analyses and chemical methods.
Dianthus
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chemistry
;
Magnetic Resonance Spectroscopy
;
Molecular Structure
;
Plant Components, Aerial
;
chemistry
;
Plants, Medicinal
;
chemistry
3.Pulmonany Carcinoma :the Analysis of Therapeutic Effect of Dual Interventional Therapy
Xiufen LIANG ; Jincheng LIU ; Guozheng YU ; Huimin NIU ; Jun HAN ; Yamin LIU ; Zhe LI ; Wenjuan ZHANG ; Wei REN
Journal of Practical Radiology 2001;0(08):-
Objective To evaluate the therapeutic effect of pulmonary carcinoma by bronchical arterial infusion (BAI) and bronchical arterial infusion with the addition of pulmonary arterial infusion (PAI).Methods Totally 69 cases with pulmonany cancinoma were treated by intervention therapy .Of them ,38 cases were undergone the bronchical arteriography and arterial infusion and 31 cases were undergone the brochical pulmonany arterial dual infusion.Results The bronchical arteryies taken part in the blood supply of neoplasmin all cases ,while the pulmonany artery only in 77% of cases .The effective ratio of BAI +PAI was 87%, and BAI was 65.7%,there were markly different (?
4.Relationship between sperm motility parameters and sperm morphology.
Yu-han MA ; Rui-zhi LIU ; Zong-ge XU ; Hong-guo ZHANG ; Zhe LI
National Journal of Andrology 2006;12(7):590-593
OBJECTIVETo evaluate the relationship between sperm motility parameters and sperm morphology.
METHODSSeven hundred and eighty-three semen samples were tested. Sperm motility parameters were analyzed by computer-aided sperm analysis (CASA) , and sperm morphology assessed by automated sperm morphology analyzer (ASMA). The cases were classified based on the World Health Organization criteria. Morphologically 241 of the samples were normal and the other 542 abnormal.
RESULTSVCL, WOB, VAP of the morphologically abnormal group were significantly higher than those of the normal group (P < 0.05, P < 0.001), while MAD, LIN, STR of the abnormal group were significantly lower (P < 0.05, P < 0.001). There were significant positive correlations between the morphologically normal sperm rates and MAD, LIN, WOB, STR, and a significant negative correlation between the morphologically normal sperm rate and ALH.
CONCLUSIONMorphological abnormality of sperm is often accompanied with weak motility, which is probably attributed more to some factors that coact on both sperm motility and morphology than to the influence of sperm morphological abnormality on sperm motility.
Adult ; Humans ; Infertility, Male ; pathology ; physiopathology ; Male ; Sperm Count ; Sperm Motility ; Spermatozoa ; pathology
5.3-dimensional position changes of periorbital structures after midface distraction osteogenesis.
Zhe-Yuan YU ; Xiong-Zheng MU ; Jia-Yi HAN
Chinese Journal of Plastic Surgery 2008;24(6):421-425
OBJECTIVETo evaluate the 3-D position changes of periorbital structures after midface distraction osteogenesis in patients with Crouzon syndrome.
METHODSThe CT data of 8 cases who had accepted the midface distraction osteogenesis following Le Fort III osteotomy were retrospectively analyzed. The patients were averagely 11.9 years old, and the CT was performed before and one year after operation. After 3-D image reconstruction, a right-hand coordinate system based on the preoperational Frankfurt Plane was then established. The pre- and post-operative positions of the superior orbit point (SOr), inferior orbit point (IOr), median orbit point (MOr), lateral orbit point (LOr), anterior ocularis point (AO), ocularis eyeball point (PO) and the four insertion ocularis rectus were documented and compared. The positions of these marked points in normal controls were also documented and compared with those in patients.
RESULTSAfter midface distraction osteogenesis, the position of AO was not changed significantly on the y-axis and z-axis, but the distance between two AO points on x-axis was reduced by 3.40 mm; IOr moved averagely 12.24 mm on y-axis and 4.25 mm on z-axis, MOr moved averagely 10.11 mm on y-axis and 2.80 mm on z-axis, LOr moved averagely 9.86 mm on y-axis and 2.31 mm on z-axis. The Inferior Rectus attachment moved averagely 3.63 mm on y-axis and 2.98 mm on z-axis. No other significant change was observed on other marked points.
CONCLUSIONSMidface distraction osteogenesis following Le Fort III osteotomy can significantly move the medial, lateral and inferior peri-orbital bone structure anteriorly and inferiorly. The eyeballs have no markedly sagittal position changes after distraction except slight medial, downwards movements and anterior-upwards rotations.
Adolescent ; Child ; Facial Bones ; diagnostic imaging ; surgery ; Female ; Humans ; Imaging, Three-Dimensional ; Male ; Orbit ; diagnostic imaging ; Osteogenesis, Distraction ; methods ; Osteotomy, Le Fort ; methods ; Tomography, X-Ray Computed
6.Experimental Study on the Up-Regulation of NF-kappa B and MMP-9 mRNA Level in HUVECs Stimulated by Serum from Children with Coronary Artery Lesions of Kawasaki Disease
Li-Sha LIU ; Zhe-Qian LI ; Cheng TAN ; Han-Qing YU
Journal of Modern Laboratory Medicine 2018;33(1):131-132,136
Objective To investigate the relationship between nuclear factor kappa B(NF-κB) and matrix metalloproteinase-9 (MMP-9) mRNA in human umbilical vein endothelial cells(HUVECs) stimulated by the serum from children with coronary artery lesions of Kawasaki disease (KD).Methods HUVECs were cultured and were divided into 4 groups:normal serum group,general fever group,Non-CALs group and CALs group.Co-Immunoprecipitation (ChIP) was used to detect the relationship between NF-κB and MMP-9,and RT-PCR was used to detect the mRNA level of MMP-9.Results Compared with control groups,NF-κB p65 could bind the promoter of MMP 9 in HUVECs cultured with 10 % serum from KD patients with coronary artery lesions.The mRNA level of MMP 9 was also up-regulated.Conclusion NF-κB p65 can promote the transcription of MMP-9 in HUVECs induced by the serum from KD patients with coronary artery lesions.
7.Identification of a novel COL2A1 variant in a pedigree affected with spondyloepiphyseal dysplasia congenita.
Yuxian WANG ; Han XIAO ; Zhe WANG ; Na ZHAO ; Yu XUE
Chinese Journal of Medical Genetics 2019;36(7):694-696
OBJECTIVE:
To explore the molecular basis for a pedigree affected with spondyloepiphyseal dysplasia congenita (SEDC).
METHODS:
The proband was subjected to whole exome sequencing. Suspected variant was verified by Sanger sequencing.
RESULTS:
All patients from the pedigree were found to carry a novel missense variant c.1394G>C (p.Gly465Ala) of the COL2A1 gene. The variant was not reported previously. Provean, Polyphen-2 and Mutation Taster software predicted that the variant is highly likely to be pathogenic.
CONCLUSION
The c.1394G>C (p.Gly465Ala) variant of the COL2A1 gene probably underlies the SEDC in this pedigree.
Asian Continental Ancestry Group
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Collagen Type II
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genetics
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Humans
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Osteochondrodysplasias
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congenital
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genetics
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Pedigree
8.Assessment of coronary artery stenosis with intracoronary Doppler guide wire and modified continuity equation method ; A comparison with dipyridamole stress Thallium-201 SPECT.
Seung Jea TAHK ; Yu Zi LI ; Jong Hoon KOH ; Myeong Ho YOON ; So Yeon CHOI ; Yo Han CHO ; Zhe Xun LIAN ; Joon Han SHIN ; Han Soo KIM ; Byung Il CHOI
Korean Circulation Journal 1999;29(2):161-173
BACKGROUND: Previous studies of animal and human experiments have shown excellent correlation between true or angiographic stenosis severity and stenosis severity calculated from intracoronary Doppler flow measurements and continuity equation method. However, there remains practical problems to be solved on its clinical application. To minimize these problems, the concept of modified continuity equation method, calculating the percent area stenosis by comparing the maximal in-stenosis flow velocity to the distal reference flow velocity, was introduced and compared with dipyridamole stress thallium-201 SPECT. METHODS: In this prospective study, 102 patients (mean age 57+/-10 years, 69 men, 33 women) with coronary artery stenoses ranging from 23-89% in percent diameter stenosis, who received coronary angiography, dipyridamole stress thallium-201 SPECT, and successful intracoronary flow velocity measurements were included. Modified continuity equation method and distal coronary flow velocity reserve were compared to the result of dipyridamole stress thallium-201 SPECT and quantitative coronary angiography. RESULTS: Measurements of adequate intralesional and stenosis distal flow velocities were successful in 102 out of 106 stenoses (96%). Minimal luminal area and percent area stenosis calculated from modified continuity equation method showed significant correlations with those of quantitative coronary angiography. Modified continuity equation method significantly underestimated the severity of stenosis than quantitative coronary angiography did. The test accuracy in relation to the result of dipyridamole stress thallium-201 SPECT were 91% in modified continuity equation method, 80% in quantitative coronary angiography and 63% in distal coronary flow velocity reserve. CONCLUSION: Application of intracoronary Doppler guide wire and modified continuity equation method appears to provide useful on-site implications for the anatomic and functional assessment of coronary artery stenosis. The modified continuity equation method would be one of the promising concepts for clinical decision making during coronary interventions.
Animals
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Constriction, Pathologic
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Coronary Angiography
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Coronary Stenosis*
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Coronary Vessels*
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Decision Making
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Dipyridamole*
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Humans
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Male
;
Phenobarbital
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Prospective Studies
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Tomography, Emission-Computed, Single-Photon*
9.Relationships between the gene polymorphisms of drug metabolizing enzymes and the outcome of the first induction chemotherapy in patients with de novo acute myeloid leukemia.
Na WANG ; Jun-Ling HAN ; Ying-Chang MI ; Zhi-Jian XIAO ; Si-Zhou FENG ; Yu-Ling ZHOU ; Jian-Xiang WANG ; Ming-Zhe HAN
Journal of Experimental Hematology 2011;19(2):327-331
The objective of this study was to investigate the correlation between the gene polymorphisms of drug metabolizing enzymes and the outcome of the first induction chemotherapy in patients with de novo acute myeloid leukemia (AML). 113 de novo AML patients were enrolled in this study. The genotypes of 11 single nucleotide polymorphisms (SNP) in drug metabolizing enzymes were detected by the SNPstream(®) Genotyping System. The correlation between the distribution of genotypes and the complete remission rate of first induction chemotherapy was analyzed by logical regression. The results showed that patients with variant genotype of CYP2D6 (rs16947) had a lower complete remission (CR) rate, as compared to those with wild type (p = 0.033, OR = 0.32, 95%CI 0.112 - 0.915); meanwhile the patients with variant genotype of GSTO2 (rs156697) had a higher CR rate as compared to those with wild type (p = 0.011, OR = 3.023, 95%CI 1.289 - 7.089). Combined analysis of the above polymorphisms, showed that patients with variant genotype of CYP2D6 and wild genotype of GSTO2 (V + W) had lower CR rates in comparison to patients with wild genotypes of both polymorphisms (p = 0.017, OR = 0.183, 95%CI 0.045 - 0.735). It is concluded that CYP2D6 (rs16947) and GSTO2 (rs156697) polymorphisms are independent factors influencing CR rates of the first induction chemotherapy in de novo AML patients.
Adolescent
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Adult
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Aged
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Antineoplastic Agents
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therapeutic use
;
Child
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Cytochrome P-450 CYP2D6
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genetics
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Female
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Genotype
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Glutathione Transferase
;
genetics
;
Humans
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Leukemia, Myeloid, Acute
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drug therapy
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enzymology
;
genetics
;
Male
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Middle Aged
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Polymorphism, Single Nucleotide
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Remission Induction
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Treatment Outcome
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Young Adult
10.Comparison between two diagnostic criteria for PBC-AIH overlap syndrome.
Jiang-Yi ZHU ; Xin-Min ZHOU ; Jian-Hong WANG ; Rui-An WANG ; Xiao-Feng HUANG ; Zhe-Yi HAN ; Yu CHEN ; Ying HAN
Chinese Journal of Hepatology 2011;19(2):132-133
Not Abstract.