Objective To observe the clinical efficacy of triple needling and centro-square needling in treating hemifacial spasm. Method Sixty-eight patients with facial spasm were randomized into a treatment group and a control group, 34 in each group. The treatment group was intervened by centro-square needling at topical Ashi points and triple needling at Yifeng (TE 17) on the affected side; the control group was intervened by regular acupuncture. The two groups were treated once a day, 10 times as a treatment course, for 3 courses in total. Cohen Albert grading scale was adopted to evaluate the spasm intensity before and after the intervention, and the clinical efficacies were then compared.Result After intervention, there was a significant difference in comparing the spasm intensity between the two groups (P<0.05). The significant improvement rate was 88.2% in the treatment group versus 50.0% in the control group, and the difference was statistically significant (P<0.05).Conclusion Triple needling plus centro-square needling is an effective method in treating hemifacial spasm. This method uses less points, but it produces a high efficacy, strong pertinence, and is easy-to-operate.

Objective To analyze the factors influencing augmentation index (AI) by examining the AI changes in patients who received continuous ambulatory peritoneal dialysis (CAPD) therapy, and evaluate the artery elastic function situation. Methods The height, weight, blood pressure, blood indicators were detected in 46 CAPD patients (CAPD group) and 42 controls (control group). AI was managed by OMRON HEM-90OOAI, and the influencing factor was analyzed. Results Systolic blood pressure(SBP),diastolic blood pressure(DBP), pulse pressure, central aortic pressure and AIp75 (AI value, when pulse was 75 beats/min) in CAPD group were significantly higher than those in control group. Within CAPD patients,the AIp75, in female was significantly higher than that in nude [(96.67±8.95 )% vs (89.89±6.46)% ,P =0.022]. Simple correlation showed positive relationship in gender, age, SBP, DBP, pulse pressure and central aortic pressure with AI; and negative relationship in height, weight and weight index with AI; while there was no relationship between AI and all kinds of chemical examination indicators or dialysis years.Multiple linear regression analysis evidenced that gender,age, height, DBP and central aortic pressure of CAPD patients had linear regression relation with AI. Conclusions It indicates a decreased artery elasticity and an increased stiffness occurs in CAPD patients. AI has tight correlation with age, height, DBP and central aortic pressure, and is an non-invasive factor thai partially reflects the arterial elasticity. Central aortic pressure is relevant with aortic elasticity and rigidity closely. Measuring central aortic pressure is very imporrant for preventing cardiovascular accident.

BACKGROUND:Articular injections of opioids are widely adopted for pain management of total knee arthroplasty, with both peri-articular and intra-articular administration routes. Recent studies have indicated that commonly used anesthetics, steroids and non-steroidal anti-inflammatory drugs are associated with potential adverse effects. Peripheral opioids are relatively safe with lower complication rates, but great controversy exits regarding the analgesic effects. OBJECTIVE:To introduce the current clinical application status and basic research progress in peri-articular and intra-articular opioid injections for postoperative analgesia of knee arthroplasties. METHODS:A computer-based search in PubMed and Embase databases was conducted for clinical and basic research articles related to pain management of knee arthroplasties using peri-articular and intra-articular injections of opioids published from January 1967 to May 2015, using the keywords of“opioids;peri-articular injection;intra-articular injection;analgesia;knee arthroplasty”in English. Irrelevant, poorly related and repetitive studies were excluded. RESULTS AND CONCLUSION:Peri-articular and intra-articular opioids injection plans vary greatly between different institutions, which does not support reliable quantitative data synthesis for a meta-analysis. Controversy exists regarding its efficacy, but there are studies supporting the usage of opioids for providing effective analgesia in a dose-dependent manner. The peri-articular administration route is associated with lower complication rates including nausea and vomiting compared to systematic injections. Applications of opioids help to avoid potential chondrocyte and stem cel cytotoxicity caused by other anesthetics, steroids and non-steroidal anti-inflammatory drugs, which is important for pain management of total knee arthroplasties. Future investigations are required to promote articular analgesic effects and time duration by exploring more advanced drug combinations and dosage forms.

Objective To investigate the clinical efficacy and safety of 5-aminolaevulinic acid-photodynamic therapy(ALA-PDT) in treatment of anorectal condyloma acuminatum.Methods 95 cases who suffered from male multiple condyloma acuminatum were randomly divided into two groups.There were 50 cases in the treatment group and 45 cases in the control group.There were no statistically significant between two groups in age,sex,course of disease,skin lesions,the size and the distribution.The treatment group was treated by photodynamic therapy combined with recombinant human interferon α-2b Gel while the control group was treated only by photodynamic therapy.The treatment time lasted 7-10 days later if the Lesion was not completely removed.Totally,1-4 treatment sessions were given.Then the rate of recovery and recurernce of the two groups were compared.Results The recovery rate in treatment group was 94.0％ (47/50) and 88.9％ (40/45) were found in control group.There were no significant difference in the two groups (x2 =0.290,P ＞ 0.05) ;The recurernce rate of the treatment group was 2.0％ (1/50) and the control group was 13.3％ (6/45),the two groups had significant differences (x2 =4.457,P =0.035).Conclusion ALA-PDT combined with recombinant human interferon α-2b Gel in the topical treatment of anorectal condyloma acuminatum is safe and effective,whcih may serve a therapeutic option for anorectal condyloma acuminatum.

ObjectiveTo screen for proteins related to invasive and metastatic potential of ovarian cancer.MethodsThe nude mice were intraperitoneally injected with the human ovarian cancer 3AO cell line.The metastatic tumors were isolated and cultured in vitro.The cell lines were established from tumors and transplanted intraperitoneally for several passages.Invasive potential of cell lines was evaluated by cell adhesion and matrigel invasion assays.Lysates from different cell lines with high or low invasive potential were analyzed by surface enhanced laser desorption/ionization-time of flight-mass spectrometry(SELDI-TOF-MS)and protein-chip array technology.The proteomic fingerprint patterns were compared. ResultsThe subclones 3AOF3 and 3AOF4 were isolated from repeated passaging in vivo and vitro,and showed higher invasive capacity than 3AO cells.By comparing their proteomic fingerprints,9 distinct proteins (3494,3831,8121,8293,9123,9332,10 184,15 436 and 17 134 at m/z value) were found,which were expressed only in 3AO cells.On the contrary,1 distinct protein (4360 at m/z value) was expressed only in 3AO F3-4 cells,and 2 distinct proteins (1842 and 3145 at m/z value) were expressed only in 3AO F2-4 cells.Additionally,6 distinct proteins (1236,5476,5649,6575,6897 and 7016 at m/z value) and a mass cluster (13 794 and 14 027 at m/z value)were expressed in all 3AO and 3AOF1-4 cells,while the amount of protein was different.ConclusionBy comparing the proteomic fingerprints of isogenic ovarian cancer cells with different invasive potential,a group of proteins are identified which are correlated with high invasive and metastatic potential.

Objective To observe the effect of continuous blood purification ( CBP ) in treatment of patients with severe viral encephalitis (SVE) combined with status epilepticus (SE).Methods Twenty-four SVE patients combined with SE were collected.Of which 15 cases received conventional treatment was control group, while 9 cases received CBP on the basis of conventional therapy was treatment group.The two groups were observed for curative effects.Results Compared with the control group, the remission time of fever, consciousness recovery, status epilepticus and mechanical ventilation were significantly shortened in treatment group ( all P<0.05 ) .The curative effects between the two groups were no significantly different.Conclusion For SVE patients with SE, the CBP treatment combined with conventional treatment can quickly relieve symptoms and improve the prognosis.

Objective To determine the optimum administration modality of netilmicin (NTM) by comparing the blood concentration, bactericidal activity, clinical effect and adverse reactions at two different regimen. MethodSerum concentration was determined by TDx (Fluorescence Polarization Immunoassy Technology). Serum bactericidal activity (SBA) was determined by microdilution method. ResultAll trough concentrations were

[Objective]To study the clinical characteristics of osteochondrosis of lumbar posterior vertebral edge(OLPV) and evaluate the efficiency of surgical treatment. [Method]From August 2000 to August 2003,41 cases of OLPV patients were treated,all cases underwent decompression of posterior approach and selective resection of the compressive things.Furthermore,1 case was treated with trans-vertebral pedicle internal fixation and inter-transverse process bone graft because of excessive decompression and preoperative lumbar instability.[Result]Low back and leg pain disappeared or obviously being relieved in all cases,and the results of histological analysis suggested the diagnosis of OLPV in all cases.Forty-one cases were followed from 5 to 8 years,average 6.8 years.No lumbar instability or spondylolisthesis was found in any case.Six cases suffered slight low back pain occasionally without obvious impaired quality of life.The preoperative JOA score was 14.73?2.06,when followed by 3 months,the score changed into 27.14?4.13,there was statistical significance between them(P

[Objective]To analyze the dynamic changing of inflammatory markers in second stage revision for infected TKA and to explore the sensitive indicators. [Methods]From June 2004 to April 2008,20 cases of infected TKA were admitted and treated by the two stages knee revision.The inflammatory markers included full blood count,erythrocyte sedimentation rate(ESR).C-reactive protein(CRP) were examined weekly,and to evaluate the dynamic changes and the most sensitive indicators.[Results]CRP was the most sensitive index for infection control,and its maximum presented in the first week post antibiotic bone cement implantation,followed by rapid reduction in the second week and there was statistical difference compared to the CRP level before surgery(t=105.84,P

[Objective]To investigate the morbidity,classification and treatment of multiple fracture around the knee in adults,and to analyze the significance of AO classification in the treatment.[Method]Multiple fractures in 167 patients from 2003 to 2007 were retrospectively analyzed and grouped according to their ages,occurring years,mechanism of injury and treatment.AO/OTA classification was assessed from the preoperative X-ray result,and healing situation was evaluated according to postoperative X-ray.[Result]All 167 multiple fractures around the knee accounted 23.69% for all lower limb fractures,with 89.2% of male and 10.8 of female.For the patient's age analysis,30-40 years old age group accounted for the largest proportion (29.34%).For the injury mechanism,traffic accident accounted for the largest proportion (53.29%).Distal femur + proximal tibia fracture accounted for 44.3%.patella + distal femur fracture accounted for 31.1%.Proximal tibia + patella accounted for 19.8%.Distal femur + proximal tibia + patella accounted for 4.8%.According to AO classification,A3 fracture was the most common in distal femoral fracture group (25.4%).B1 fracture was the most common in proximal tibia fracture group (21.7).C3 fracture was the most common in patella fracture (31.2).Tthe final good-to-excellent rate was 85.63%.[Conclusion]Distal femoral fracture combined with proximal tibia fracture was the most common in multiple fractures around the knee.The common fracture type is supracondylar fracture in femur,transverse patella and tibia.Application of nail,plate,wire and tension band,et al.can achieve good fixation.