Objective To explore the effect of laparoscopy and laparotomy surgery on immune function related indicators in ovarian benign tumor patients. Methods From January 2015 to January 2016, 86 cases of ovarian benign tumor patients were set as study objects. All the patients were randomly divided into control group and observation group, 43 cases in each. Patients in control group were given traditional laparotomy surgery, while patients in observation group were given laparoscopic surgery. The changes of immune function related indexes before and after operation were recorded, before operation, day 1 and day 3 postoperative, number of peripheral white blood cells (WBC), neutrophil ratio (NGR), immunoglobulin (IgA, IgG, IgM) levels, C reactive protein (CRP) content and the distribution of T lymphocyte subsets (CD3+, CD4+ and CD3+/CD4+ ratio) distribution were respectively determined, and the incidence of postoperative complications was recorded, and were further statistical analyzed. Results There was no significant difference in immunoglobulin IgA, IgG and IgM between the twogroups before operation (P > 0.05). Postoperative, only the IgG content of the two groups was significantly lower than that before the operation (P < 0.05 ); The levels of WBC, NGR and CRP in the control group patients in the昀rst day and the third day after surgery were significantly increased compared with preoperative period, while CRP in the observation group was higher than that before the operation, there was significant difference between the two groups (P < 0.05). There was no significant difference in CD3+, CD4+ and CD3+/CD4+ ratios between the two groups before operation (P > 0.05), the difference between the two groups was significant (P < 0.05). The complication rate of the observation group after operation was 6.98%, the control group was 16.28%. Conclusion Clinical use of laparoscopic treatment of benign ovarian tumors relative to the traditional laparotomy can effectively reduce the immune system of patients with immune function, with high safety, contribute to the recovery of patients, it is worthy of clinical promoting.

OBJECTIVE:To observe the clinical efficacy and safety of noninvasive mechanical ventilation combined with aero-sol inhalation drug in the treatment of AECOPD complicating with respiratory failure. METHODS:90 patients diagnosed as AECO-PD complicating with respiratory failure were selected and randomly divided into observation group and control group,with 45 cas-es in each group. Both groups received routine treatment as oxygen supply at low flow rate,anti-infection,relieving asthma,venti-lation,cardiotonic,spasmolysis and nutritional support. Control group received noninvasive mechanical ventilation;observation group was additionally given aerosol inhalation of Budesonide atomized liquid+terbutaline+ipratropium bromide,bid. Both groups received 7 d of treatment. Clinical efficacy of 2 groups were compared as well as pH value,PaCO2,PaO2,HR,RR and PEF lev-els before and after treatment. The occurrence of ADR was observed. RESULTS:Total effective rate of observation group was 93.3%,which was significantly higher than 73.3% of control group,with statistical significance(P<0.05). There was no statisti-cal significance in blood gas analysis indexes,HR,RR and REF between 2 groups before treatment (P>0.05). pH value,PaO2 and PEF of 2 groups increased after treatment,while PaO2,HR and RR decreased;the observation group was better than the con-trol group,with statistical significance(P<0.05). No obvious ADR was found in 2 groups. CONCLUSIONS:Noninvasive me-chanical ventilation combined with aerosol inhalation drug is effective in the treatment of AECOPD complicating with respiratory failure,and can effectively improve blood gas indexes,HR,RR and PEF with good safety.

Objective:To explore the effective treatment to the depression after silent cerebral infarction(SCI).Methods:Seventy-three patients suffering from depression after SCI were treated for three moths with a mono-blind experiment.Results:There was significant difference between the effect of antidepressant and the effect of nonantideressant( P

AIM: To investigate the changes of serum angiogenic factors including vascular endothelial growth factor (VEGF) and basic fibroblast growth factor ( bFGF) in Mongolia patients with diabetic retinopathy ( DR) and to explore their roles in the progress of DR.
METHODS: Eighty - three patients with diabetes were divided into three groups according to the retinopathy, no DR group ( NDR, 25 cases), background DR ( NPDR, 31 cases) and proliferative DR group (PDR, 27 cases), and 36 age - matched healthy people ( control group) were selected. Serum concentrations of VEGF and bFGF were measured using ELISA method.
RESULTS: In Mongolia patients with diabetes, the serum concentrations of VEGF and bFGF were higher than those in control group; the serum concentrations of VEGF and bFGF in PDR group was higher than those of NDR and NPDR group (P< 0. 05); the concentrations of VEGF and bFGF in NPDR group was higher than that of NDR group (P<0. 05).
CONCLUSION: High level of VEGF and bFGF may be the important pathogenic factors for DR in Mongolia patients.

Objective To determine the clinical features of Klebsiella pneumoniae pneumonia and antimicrobial resistance of Klebsiella pneumoniae, providing the basis for rational use of antibiotics. Methods Eighty-six strains of Klebsiella pneumoniae were isolated from the sputum specimen which collected from the inpatients in our hospital from Dec 2008 to Dec 2010,which were identified with Bio Merieum Vitek 2. A total of 86 children were enrolled in the study, which were divided into hospital infection/colonization group (n = 68) and community infection group (n = 18). Clinical information were recorded. Extended spectum β-lactamase (ESBLs) and antibiotic susceptibility of Klebsiella pneumoniae were determined. Results Klebsiella pneumoniae pneumonia in children mainly occured in infants, especially in infants with basic diseases.Clinical manifestations of Klebsiella pneumoniae pneumonia had no much difference from common pneumonia. The detection rate of ESBLs was high(76. 7%). ESBLs were found in 64 strains of the hospital infection/colonization group (94. 1% ,64/68) ,while in 2 strains of the community infection group (11. 1% ,2/18).There was a significant difference between the two groups (P ＜0. 01). The drug resistance of hospital infection/colonization group was significantly higher than that of community infection group. The strains of hospital infection/colonization group were sensitive with cefotan, piperacillin/tazobactam, carberpenem, amikacin and ciprofioxacin only, while were highly resistant to penicillins, cephalosporins and monoamine antibiotics.The average hospital stay of the hospital infection/colonization group was (15.0 ± 7. 1) d, while the other was (8. 2 ± 3. 8) d. There was a very significant difference between the two groups in average length of stay (P＜0.01). Conclusion Klebsieila pneumoniae pneumonia in children mainly occurs in infants with basic diseases. The symptoms are untypical. Klebsiella pneurmoniae multi-drug resistance is a serious problem,which must be paid attention to. We should make a difference between the two groups when antibiotics were prescribed.

Objective To observe the clinical efficacy and adverse reactions of docetaxel and carboplatin 21 day regimen (TC)as second -or third -line in treatment of patients with advanced triple -negative breast cancer (ATNBC).Methods 37 ATNBC patients with anthracyclines resistance were given docetaxel 70mg/m2 ,the first day of intravenous drip,carboplatin AUC =5,through intravenous drip on the first day,21 days for a course of treatment, until disease progression or not tolerated or most received six cycles of chemotherapy.Results 147 cycles of chemo-therapy were completed in 37 patients,and the median chemotherapy cycle was 4 cycles.The efficacy and adverse reactions could be evaluated in all patients.Among them,complete remission (CR)in 0 patient,partial remission (PR)in 12 patients (32.43%),stable (SD)in 11 patients (29.73%),and progression (PD)in 14 patients (41.18%).The effective rate(CR +PR)was 32.43%.The disease control rate (CR +PR +SD)was 62.15%,median TTP was 3.9 months (95%CI:4 -8months),and median OS was 10 months.Until the end of follow -up,there were 3 patients with no progress.The main adverse reactions were hematological toxicity (51.53%)and gastrointestinal reaction (56.76%).Conclusion TC regimen is effective and safe in the treatment of patients with ATNBC.

Objective To summarize the nursing experience of a child with serious skin problems at the puncturing site of peripherally inserted central catheter (PICC) for methotrexate (MTX) infusion.MethodsA pediatric patient from the Hematology Ward of Beijing University People''s Hospital was found to be with serious skin problems at the puncturing site of PICC for MTX infusion.Local irradiation with ultraviolet, disinfection of local skin with iodine volts and 0.9％ sodium chloride injection, coverage of the broken skin with alginate dressing, and fixation of PICC with sterile gauzes were applied.Results After 22 days of nursing, the pain and itching at the broken skin around PICC disappeared.The skin returned normal, without exudates and prolapse of catheter.Conclusions After MTX use via PICC, the children can easily become irritable due to local symptoms and their own feelings after skin allergy.Meanwhile, prolonged treatment and nursing may make the child cry and increase the stress of parents.Caregivers needs to explain the related factors of allergy to the children and their families, and give the correct nursing intervention, so as to improve local allergy and facilitate the retention of PICC.

Objective Via observing concentration changes of interleukin(IL)-1β, IL-6 and IL-18 in hippocampus of pilocarpine treated rats after intranasal delivery of transforming growth factor-β1(TGF-β1), to explore the possi-ble anti-epileptogenic and neuro-protective role of TGF-β1 in the epiletogenesis progression. Methods Three hours after pilocarpine induced status epilepticus, rats were treated with recombinant human TGF-β1 or the same volume of PBS by intranasal administration. Animals were executed, harvested at 24, 48, 72 h after SE individual-ly. Using the methods of the enzyme-linked immunosorbent assay (ELISA) and immunohistochemisty (IHC), the concentrations and distribution of IL-1β, IL-6, IL-18 were detected in hippocampus. Results TGF-β1 could re-duce the expression of IL-1β and IL-6 in the hippocampus of pilocarpine treated rats effectively (P<0.05 or P<0.01), however, TGF-β1 did not affect the expression of IL-18 significantly( P >0.05). Conclusion By the supplementation of intranasal TGF-β1, classical pro-inflammatory cytokine ( IL-1β, IL-6) are remarkably sup-pressed, TGF-β1 may exert antiepileptogenic and neuropretective effects.

Objective To study the clinical significance of hypersensitive C‐reactive protein(hs‐CRP) in acute coronary syndrome (ACS) .Methods 45 patients with ACS were selected as the ACS group ,including 26 cases of acute myocardial infarction (AMI group) and 19 cases of unstable angina pectoris (UAP group) ;30 patients with stable angina pectoris(SAP) were selected as the SAP group and 30 health people with healthy physical examination in the outpatient department as the control group .The latex‐en‐hanced immunoturbidimetry was adopted to detect the serum hs‐CRP levels in all selected subjects and the detection results were compared .Results The serum hs‐CRP level of ACS and SAP groups were significantly higher than that of the control group with statistical difference (P< 0 .01) .The hs‐CRP level of AMI and UAP groups was higher than that of the SAP group with statistical difference (P< 0 .05) .Conclusion hs‐CRP is an independent risk factor for ACS and one of the most powerful predictors ,able to quickly and accurately conduct early diagnosis ,risk stratification ,condition monitoring and prognosis judgment in the patients with ACS .

In recent years,China's science research has entered the high-speed development period,which leads to the increase of the investment of science research funds.At the same time,the safety and effectiveness of the science research management and the funds has caused public concern.So the science management of research work including the use of science research funds is of great significance.This paper will analyze the risk of key positions in science research management and put forward corresponding countermeasures,combining the writer's practical experience in hospital.