Humans ; Occupational Diseases ; diagnosis

OBJECTIVE: To discuss the biosafety and biocompatibility of biomaterials. METHODS: A computer-based online search of CNKI, CBM and VIP as well as manual search were performed to collect articles about random cell control experiments and animal experiments of biological material biocompatibility published between 1990 and 2008. A total of 28 Chinese literatures were collected, and 7 were included mainly involving the cytotoxicity test methods and blood compatibility of the experimental medium, experimental grouping, experimental materials, methods of observation, experimental results, and experimental conclusion. In addition, biocompatibility of the biological material was analyzed to summarize the biocompatibility of the biological safety. RESULTS: Based on experiments of biosafety and biocompatibility of biomaterials, the cytotoxicity test and blood compatibility of various biomaterials, such as collagen, chitosan, magnetic nanoparticle, metal vascular stent, sulphurated siliastic medical grade silicon rubber, polyurethane, ceramic coatings by micro-arc oxidation, are essential for the biological safety. The experimental results have shown that the biomaterials have good biocompatibility. CONCLUSION: A variety of biological materials present good biocompatibility, including collagen, chitosan, magnetic nanoparticle, metal vascular stent, sulphurated siliastic medical grade silicon rubber, polyurethane, ceramic coatings by micro-arc oxidation, based on the evaluation criteria.

Background and purpose: MicroRNA(miRNA) is a class of small non-coding RNA playing an important regulatory role in many diseases. In this study, we explore the levels of miR-192 and zinc ifnger E-box binding homeobox 2 (ZEB2) in CRC, the clinical signiifcance of miR-192 and the correlation between the expression of miR-192 and ZEB2 protein. Methods: The expression levels of miR-192 and ZEB2 mRNA in 30 colorectal carcinoma samples, 30 corresponding cancer-adjacent tissue samples (from the edge of tumor≥5 cm), 25 colorectal adenoma samples, and 15 normal tissue samples were quantiifed using quantitative real-time polymerase chain reaction (qRT-PCR). ZEB2 protein expression was determined using Western blot. The relationship between the miR-192 and clinicopathological factors, miR-192 and ZEB2 protein expression was analyzed. Results:Signiifcant upregulation of miR-192 expression and reduction of ZEB2 mRNA and protein expression were identiifed in CRC tissues, compared to cancer-adjacent tissues, colorectal adenoma samples, and normal tissues (P<0.05). Low miR-192 levels were signiifcantly associated with lymph node (P=0.021) and distant metastasis (P=0.023). An inverse relationship between miR-192 and ZEB2 protein expression was identified in CRC group (r=-0.365, P<0.05). Conclusion: MiR-192 downexpression was correlated with CRC metastasis. MiR-192 may play a role in the development and progression of CRC through ZEB2.

Objective To explore the recurrence-prevention effect of oral contraceptive and levonorgestrel-releasing intrauterine system (Mirena) after hysteroscopic endometrial polypectomy.Methods One hundred and seventy-four patients who received hysteroscopic endometrial polypectomy were enrolled in this research.The patients were informed and divided into three groups according to their selection:51 cases were given oral contraceptive since one month until 12 months after the surgery (oral contraceptive group); 60 cases were received Mirena since one month after the surgery (Mirena group) ; and 64 cases were received no treatment after the surgery (control group).The groups were followed up at 3,6,12 months after the surgery and compared the recurrence rate,menstruation,level of hemoglobin and complication rate.Results The recurrence rate in oral contraceptive group,Mirena group and control group was 3.9％(2/51),3.3％ (2/60) and 19.0％ (12/63),respectively.The recurrence rate in control group was higher than that in oral contraceptive group and Mirena group,and there was significant difference (P ＜ 0.05).The proportion of low menstruation volume in control group was lower than that in oral contraceptive group and Mirena group,and there was significant difference (P ＜ 0.05).The level of hemoglobin in control group was lower than that in oral contraceptive group and Mirena group [(124.55 ±9.33) g/L vs.(133.71 ± 11.03),(135.89 ±6.88) g/L],and there was significant difference (P ＜ 0.05).The incidence of complication in Mirena group [18.3％ (11/60)] was less than that in oral contraceptive group[35.3％(18/51)],and there was significant difference (P ＜ 0.05).Conclusions Oral contraceptive and Mirena after hysteroscopy for endometrial polys significantly decrease the recurrence rate.The complication rate of Mirena is lower and it is a safe and effective way to treat and prevent the endometrial polyp.

Venous valve plays an important role in regulating the venous flow,especially in preventing the blood backflow.It has heen observed that when deep vein thrombosis and dissolved,the valves in the lesions had been destroied or dysfunction.The mechanism of damage of vein valve has not been fully clarified,but as we know,it's common that the inflammatory reaction,due to many unfavorable factors,can cause thrombosis.On the other hand,deep vein thrombosis could bring out the inflammatory reaction as well.So the author will review the development of researching of the destruction of venous valves in recent years.

Objective: To study the antifungal activity of triazole alcohols by introduction of n-butyl and triazole as side chains. Methods: A total of 16 compounds of 1-(1H-1, 2, 4-triazole-1-yl)-2-(2, 4-difluorophenyl)-3-substituted-2-propanol were synthesized and characterized by 1HNMR and LC-MS. The in vitro antifungal activities of the compounds were determined by tests with 8 human pathogenic fungi. Results: It was found that all the 16 title compounds exhibited antifungal activities, and compounds 7b, 7d, 7e and 7i had antifungal activities stronger than fluconazole, similar to itraconazole. Conclusion: Introduction of n-butyl and triazole side chain can confer antifungal activities to the compounds.

Objective To evaluate the value of DSA image mosaic in arteriography in lower extremities. Methods 22 suspected cases were examined by bolus chase, and then the images were mosaiced to a full picture after processing by Paste to judge the image effects of vessels. Results This method could display the whole image by one injection of contrast media. Vary lesions were found in the 22 cases. Conclusion This method can display the lower extremities lesions directly, which is better than traditional DSA.

Objective To compare the safety and efficiency of non -ionic monomer diagnostic opaque, iodixanol (Visipaque) and that of non -ionic disomer diagnostic opaque, iohexol (Omnipaque). Methods By comparing retrospectively the DSA opacification of 60 patients from 2006 to 2007, patients′ indispositions, adverse reactions and pictorial efficiency were recorded respectively. Results In the control group, iodixanol showed less indisposition caused by injection than iohexol and there′ s no obvious difference of indisposition. Both images met diagnostic requirements. Conclusion Both iodixanol and iohexol are safe, efficient and well -tolerant diagnostic opaque. Iodixanol is better than iohexol in indisposition caused by injection.

Objective Experimental research demonstrated that oxidative damage leads to formation of cataract in rats and its machanism is the decline of activities of superoxide dismutase(SOD), glutathione peroxidase(GSH-PX) and catalase(CAT) . Aaspirin can improve the antioxidative ability of lens. The purpose of this study was to observe the inhibition of aspirin on D-Galactose-induced cataractous lenses of rats. Methods Galactose cataract model was established in 40 cleaning Wistar rals by intraperitoneal injection of 20 mL/kg 80% D-Galactose for 10 days. The models were divided into model group (20 rats) and aspirin group(20 rats). 150 mg/kg of aspirin was administered immediately by gastrogavaging in aspirin group for 20 days. Other 20 normal Wistar rats were as control group. At day 3, 6, 10, 14, 20, the transparency of rat lenses was observed under the slit lamp microscopy. At day 5 after experiment, the ultrastructure of the lenses was examined and evaluated under the scanning electron microscopy. The activities of SOD, GSH-PX and CAT were detected by Coomassie Brilliant Blue color comparator, respectively. The use of experimental animal followed the Regulations for the Administration of Affair Concerning Experimental Animals by State Science and Technology Commission. Results All lenses were transparent in the rats of control group. The degree of lens opacity was more mild in asprin group compared with model group. 25. 00%, 41. 67%, 58. 33%, 83. 33% of lenses in aspirin group showed swelling at day 6, 10, 14, 20, respectively, but 65% lenses were opacity in model group on day 3 and 100% lenses were nuclear cataracts in 6 days. The structure of lenses was normal in control group, but the process number, fiber thickness and fiber density of lens were significantly increased in model group compared with control group (P ＜0. 05), and only process number was increased in asprin group. The activities of SOD, GSH-PX and CAT in lens of model group were obviously lower than in normal control group(P＜0. 05), but those in asprin group were significantly increased in comparison with model group(P ＜0. 05). Conclusion Aspirin could protect lenses of rats against oxidative damage by elevating activities of SOD, GSH-PX and CAT in lens and inhibiting the generation and development of galactose-induced cataract at early stage of cataract.

As pattern recognition receptors of innate immunity,Toll-like receptors (TLRs) play an important role in the identification of pathogens,regulation of adaptive immunity and signal transduction.With more exploration of the mechanism of microbial keratitis and the acute or chronic rejection after penetrating keratoplasty,the induction and regulation of TLRs on the corneal immune response is increasingly concerned.Up to now,more and more studies of immune signal pathway of TLRs in mediating inflammatory response of bacterial keratitis,fungal keratitis,viral keratitis and graft rejection have been documented.To regulate the expression of TLRs is undoubtedly available for lightening and arresting the immuno-inflammatory response.Here,the current knowledge about TLRs,their signal transduction pathway and their regulation on corneal immunity are summarized,aiming to discuss their significant effects and the potential therapeutic targets.